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Protein

Peptide deformylase 1

Gene

defA

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions (By similarity).By similarity

Catalytic activityi

Formyl-L-methionyl peptide + H2O = formate + methionyl peptide.

Cofactori

Fe2+By similarityNote: Binds 1 Fe2+ ion.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi90IronBy similarity1
Metal bindingi132IronBy similarity1
Active sitei133By similarity1
Metal bindingi136IronBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processProtein biosynthesis
LigandIron, Metal-binding

Enzyme and pathway databases

BioCyciBSUB:BSU15720-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Peptide deformylase 1 (EC:3.5.1.88)
Short name:
PDF 1
Alternative name(s):
Polypeptide deformylase 1
Gene namesi
Name:defA
Synonyms:def, yloK
Ordered Locus Names:BSU15720
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000827391 – 160Peptide deformylase 1Add BLAST160

Proteomic databases

PaxDbiP94462
PRIDEiP94462

Interactioni

Protein-protein interaction databases

IntActiP94462, 1 interactor
STRINGi224308.Bsubs1_010100008681

Structurei

3D structure databases

ProteinModelPortaliP94462
SMRiP94462
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the polypeptide deformylase family.Curated

Phylogenomic databases

eggNOGiENOG4108Z02 Bacteria
COG0242 LUCA
HOGENOMiHOG000243509
InParanoidiP94462
KOiK01462
OMAiVCIQHEI
PhylomeDBiP94462

Family and domain databases

CDDicd00487 Pep_deformylase, 1 hit
Gene3Di3.90.45.10, 1 hit
HAMAPiMF_00163 Pep_deformylase, 1 hit
InterProiView protein in InterPro
IPR023635 Peptide_deformylase
IPR036821 Peptide_deformylase_sf
PANTHERiPTHR10458 PTHR10458, 1 hit
PfamiView protein in Pfam
PF01327 Pep_deformylase, 1 hit
PIRSFiPIRSF004749 Pep_def, 1 hit
PRINTSiPR01576 PDEFORMYLASE
SUPFAMiSSF56420 SSF56420, 1 hit
TIGRFAMsiTIGR00079 pept_deformyl, 1 hit

Sequencei

Sequence statusi: Complete.

P94462-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVKKVVTHP AEVLETPAET VTVFDKKLKK LLDDMYDTML EMDGVGLAAP
60 70 80 90 100
QIGILKRAAV VEIGDDRGRI DLVNPEILEK SGEQTGIEGC LSFPNVYGDV
110 120 130 140 150
TRADYVKVRA FNRQGKPFIL EARGFLARAV QHEMDHLDGV LFTSKISKYY
160
TEDELADMEG
Length:160
Mass (Da):17,775
Last modified:May 1, 1997 - v1
Checksum:iC73DBC266FDD98FC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y10304 Genomic DNA Translation: CAA71349.1
Y13937 Genomic DNA Translation: CAA74262.1
AL009126 Genomic DNA Translation: CAB13445.1
PIRiF69613
RefSeqiNP_389454.1, NC_000964.3
WP_003232077.1, NZ_JNCM01000035.1

Genome annotation databases

EnsemblBacteriaiCAB13445; CAB13445; BSU15720
GeneIDi938819
KEGGibsu:BSU15720
PATRICifig|224308.179.peg.1712

Similar proteinsi

Entry informationi

Entry nameiDEF1_BACSU
AccessioniPrimary (citable) accession number: P94462
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 1, 1997
Last modified: May 23, 2018
This is version 122 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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