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Protein

Peptide deformylase 1

Gene

defA

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions (By similarity).By similarity

Catalytic activityi

Formyl-L-methionyl peptide + H2O = formate + methionyl peptide.

Cofactori

Fe2+By similarityNote: Binds 1 Fe2+ ion.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi90IronBy similarity1
Metal bindingi132IronBy similarity1
Active sitei133By similarity1
Metal bindingi136IronBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciBSUB:BSU15720-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Peptide deformylase 1 (EC:3.5.1.88)
Short name:
PDF 1
Alternative name(s):
Polypeptide deformylase 1
Gene namesi
Name:defA
Synonyms:def, yloK
Ordered Locus Names:BSU15720
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000827391 – 160Peptide deformylase 1Add BLAST160

Proteomic databases

PaxDbiP94462.

Interactioni

Protein-protein interaction databases

IntActiP94462. 1 interactor.
STRINGi224308.Bsubs1_010100008681.

Structurei

3D structure databases

ProteinModelPortaliP94462.
SMRiP94462.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the polypeptide deformylase family.Curated

Phylogenomic databases

eggNOGiENOG4108Z02. Bacteria.
COG0242. LUCA.
HOGENOMiHOG000243509.
InParanoidiP94462.
KOiK01462.
OMAiMYREGCL.
PhylomeDBiP94462.

Family and domain databases

CDDicd00487. Pep_deformylase. 1 hit.
Gene3Di3.90.45.10. 1 hit.
HAMAPiMF_00163. Pep_deformylase. 1 hit.
InterProiIPR023635. Peptide_deformylase.
[Graphical view]
PANTHERiPTHR10458. PTHR10458. 1 hit.
PfamiPF01327. Pep_deformylase. 1 hit.
[Graphical view]
PIRSFiPIRSF004749. Pep_def. 1 hit.
PRINTSiPR01576. PDEFORMYLASE.
SUPFAMiSSF56420. SSF56420. 1 hit.
TIGRFAMsiTIGR00079. pept_deformyl. 1 hit.

Sequencei

Sequence statusi: Complete.

P94462-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVKKVVTHP AEVLETPAET VTVFDKKLKK LLDDMYDTML EMDGVGLAAP
60 70 80 90 100
QIGILKRAAV VEIGDDRGRI DLVNPEILEK SGEQTGIEGC LSFPNVYGDV
110 120 130 140 150
TRADYVKVRA FNRQGKPFIL EARGFLARAV QHEMDHLDGV LFTSKISKYY
160
TEDELADMEG
Length:160
Mass (Da):17,775
Last modified:May 1, 1997 - v1
Checksum:iC73DBC266FDD98FC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y10304 Genomic DNA. Translation: CAA71349.1.
Y13937 Genomic DNA. Translation: CAA74262.1.
AL009126 Genomic DNA. Translation: CAB13445.1.
PIRiF69613.
RefSeqiNP_389454.1. NC_000964.3.
WP_003232077.1. NZ_JNCM01000035.1.

Genome annotation databases

EnsemblBacteriaiCAB13445; CAB13445; BSU15720.
GeneIDi938819.
KEGGibsu:BSU15720.
PATRICi18974951. VBIBacSub10457_1667.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y10304 Genomic DNA. Translation: CAA71349.1.
Y13937 Genomic DNA. Translation: CAA74262.1.
AL009126 Genomic DNA. Translation: CAB13445.1.
PIRiF69613.
RefSeqiNP_389454.1. NC_000964.3.
WP_003232077.1. NZ_JNCM01000035.1.

3D structure databases

ProteinModelPortaliP94462.
SMRiP94462.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP94462. 1 interactor.
STRINGi224308.Bsubs1_010100008681.

Proteomic databases

PaxDbiP94462.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB13445; CAB13445; BSU15720.
GeneIDi938819.
KEGGibsu:BSU15720.
PATRICi18974951. VBIBacSub10457_1667.

Phylogenomic databases

eggNOGiENOG4108Z02. Bacteria.
COG0242. LUCA.
HOGENOMiHOG000243509.
InParanoidiP94462.
KOiK01462.
OMAiMYREGCL.
PhylomeDBiP94462.

Enzyme and pathway databases

BioCyciBSUB:BSU15720-MONOMER.

Family and domain databases

CDDicd00487. Pep_deformylase. 1 hit.
Gene3Di3.90.45.10. 1 hit.
HAMAPiMF_00163. Pep_deformylase. 1 hit.
InterProiIPR023635. Peptide_deformylase.
[Graphical view]
PANTHERiPTHR10458. PTHR10458. 1 hit.
PfamiPF01327. Pep_deformylase. 1 hit.
[Graphical view]
PIRSFiPIRSF004749. Pep_def. 1 hit.
PRINTSiPR01576. PDEFORMYLASE.
SUPFAMiSSF56420. SSF56420. 1 hit.
TIGRFAMsiTIGR00079. pept_deformyl. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDEF1_BACSU
AccessioniPrimary (citable) accession number: P94462
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 1, 1997
Last modified: November 2, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.