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P94453 (ALF_GEOSE) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Fructose-bisphosphate aldolase

Short name=FBP aldolase
Short name=FBPA
EC=4.1.2.13
Alternative name(s):
Fructose-1,6-bisphosphate aldolase
Gene names
Name:fba
OrganismGeobacillus stearothermophilus (Bacillus stearothermophilus)
Taxonomic identifier1422 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

Protein attributes

Sequence length287 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3-phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis By similarity.

Catalytic activity

D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.

Cofactor

Binds 2 zinc ions per subunit. One is catalytic and the other provides a structural contribution By similarity.

Pathway

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.

Sequence similarities

Belongs to the class II fructose-bisphosphate aldolase family.

Ontologies

Keywords
   Biological processGlycolysis
   LigandMetal-binding
Zinc
   Molecular functionLyase
   PTMPhosphoprotein
Gene Ontology (GO)
   Biological_processfructose 1,6-bisphosphate metabolic process

Inferred from electronic annotation. Source: InterPro

glycolytic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionfructose-bisphosphate aldolase activity

Inferred from electronic annotation. Source: UniProtKB-EC

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 287287Fructose-bisphosphate aldolase
PRO_0000178704

Regions

Region210 – 2123Dihydroxyacetone phosphate binding By similarity
Region231 – 2344Dihydroxyacetone phosphate binding By similarity

Sites

Active site851Proton donor By similarity
Metal binding861Zinc 1; catalytic By similarity
Metal binding1071Zinc 2 By similarity
Metal binding1371Zinc 2 By similarity
Metal binding1811Zinc 1; catalytic By similarity
Metal binding2091Zinc 1; catalytic By similarity
Binding site501Glyceraldehyde 3-phosphate By similarity
Binding site1821Dihydroxyacetone phosphate; via amide nitrogen By similarity

Amino acid modifications

Modified residue2121Phosphothreonine By similarity
Modified residue2341Phosphothreonine By similarity

Sequences

Sequence LengthMass (Da)Tools
P94453 [UniParc].

Last modified July 15, 1999. Version 3.
Checksum: 7D9831D2CEDD07C3

FASTA28730,818
        10         20         30         40         50         60 
MSLVSMKEML NEALRGKYAV GQFNINNLEW TQAILAAAEE EKSPVILGVS EGAARYMGGF 

        70         80         90        100        110        120 
KTVVNMVKGL MEDMNITVPV AIHLDHGSSF EKCKAAIDAG FTSVMIDASH HPFEENVRIT 

       130        140        150        160        170        180 
SQVVEYAHAR GVSVEAELGI VGGQEDDVVG EGVIYADPKE CEELVKRTGI DCLAPALGSV 

       190        200        210        220        230        240 
HGPYKGEPKL GFAEMEKIRD LTGIPLVLHG GTGIPTEQIQ RAISLGTSKI NVNTENQIAF 

       250        260        270        280 
TKAVRELLAK DPNVYDPRKI IGPGRDAIKA TVIGKMREFG SSGKAAQ 

« Hide

References

[1]Desai S.Y., Littlechild J.A.
Submitted (FEB-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Y11135 Genomic DNA. Translation: CAA72018.1.

3D structure databases

ProteinModelPortalP94453.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00109; UER00183.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
InterProIPR013785. Aldolase_TIM.
IPR011289. Fruc_bis_ald_class-2.
IPR000771. Ketose_bisP_aldolase_II.
[Graphical view]
PfamPF01116. F_bP_aldolase. 1 hit.
[Graphical view]
PIRSFPIRSF001359. F_bP_aldolase_II. 1 hit.
TIGRFAMsTIGR00167. cbbA. 1 hit.
TIGR01859. fruc_bis_ald_. 1 hit.
PROSITEPS00602. ALDOLASE_CLASS_II_1. 1 hit.
PS00806. ALDOLASE_CLASS_II_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameALF_GEOSE
AccessionPrimary (citable) accession number: P94453
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 15, 1999
Last modified: June 11, 2014
This is version 69 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways