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Protein

Pyruvate carboxylase

Gene
N/A
Organism
Geobacillus stearothermophilus (Bacillus stearothermophilus)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Protein predictedi

Functioni

Catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second.UniRule annotation

Catalytic activityi

ATP + pyruvate + HCO3- = ADP + phosphate + oxaloacetate.UniRule annotation

Cofactori

biotinUniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei121ATPUniRule annotation1
Binding sitei205ATPUniRule annotation1
Binding sitei240ATPUniRule annotation1
Active sitei296UniRule annotation1
Metal bindingi543Divalent metal cationUniRule annotation1
Binding sitei615SubstrateUniRule annotation1
Metal bindingi712Divalent metal cation; via carbamate groupUniRule annotation1
Metal bindingi741Divalent metal cationUniRule annotation1
Metal bindingi743Divalent metal cationUniRule annotation1
Binding sitei876SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigaseUniRule annotationImported
LigandATP-bindingUniRule annotation, BiotinUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding, PyruvateImported

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate carboxylaseUniRule annotation (EC:6.4.1.1UniRule annotation)
OrganismiGeobacillus stearothermophilus (Bacillus stearothermophilus)Imported
Taxonomic identifieri1422 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

Structurei

3D structure databases

ProteinModelPortaliP94448.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 457Biotin carboxylationInterPro annotationAdd BLAST453
Domaini125 – 321ATP-graspInterPro annotationAdd BLAST197
Domaini534 – 802Pyruvate carboxyltransferaseInterPro annotationAdd BLAST269
Domaini1071 – 1146Lipoyl-bindingInterPro annotationAdd BLAST76

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
3.30.1490.20. 1 hit.
InterProiView protein in InterPro
IPR013785. Aldolase_TIM.
IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR005481. BC-like_N.
IPR011764. Biotin_carboxylation_dom.
IPR005482. Biotin_COase_C.
IPR000089. Biotin_lipoyl.
IPR003379. Carboxylase_cons_dom.
IPR005479. CbamoylP_synth_lsu-like_ATP-bd.
IPR016185. PreATP-grasp_dom.
IPR000891. PYR_CT.
IPR005930. Pyruv_COase.
IPR011054. Rudment_hybrid_motif.
IPR011053. Single_hybrid_motif.
PfamiView protein in Pfam
PF02785. Biotin_carb_C. 1 hit.
PF00289. Biotin_carb_N. 1 hit.
PF00364. Biotin_lipoyl. 1 hit.
PF02786. CPSase_L_D2. 1 hit.
PF00682. HMGL-like. 1 hit.
PF02436. PYC_OADA. 1 hit.
PIRSFiPIRSF001594. Pyruv_carbox. 1 hit.
SMARTiView protein in SMART
SM00878. Biotin_carb_C. 1 hit.
SUPFAMiSSF51230. SSF51230. 1 hit.
SSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01235. pyruv_carbox. 1 hit.
PROSITEiView protein in PROSITE
PS50975. ATP_GRASP. 1 hit.
PS50979. BC. 1 hit.
PS50968. BIOTINYL_LIPOYL. 1 hit.
PS00866. CPSASE_1. 1 hit.
PS00867. CPSASE_2. 1 hit.
PS50991. PYR_CT. 1 hit.

Sequencei

Sequence statusi: Complete.

P94448-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTRRIRKVL VANRGEIAIR VFRACTELGI RTVAIYSKED VGSYHRYKAD
60 70 80 90 100
EAYLVGEGKK PIEAYLDIEG IIEIAKAHDV DAIHPGYGFF SENIQFAKRC
110 120 130 140 150
REEGIIFFGP NENHLDMFGD KVKARHAAVN AGIPVIPGSD GPVDGLEDVV
160 170 180 190 200
AFAEAHGYPI IIKAALGGGG RGMRFVRSKS EVKEAFERAK SEAKAAFGSD
210 220 230 240 250
EVYVEKLIEN PKHIEVQILG DYEGNIVHLY ERDCSVQRRH QKVVEVAPSV
260 270 280 290 300
SLSDELRQRI CEAAVQLMRS VGYVNAGTVE FLVSGDEFYF IEVNPRIQVE
310 320 330 340 350
HTITEMITGI DIVQSQILIA DGCSLHSHEV GIPKQEDIRI NGYAIQSRVT
360 370 380 390 400
TEDPLNNFMP DTGKIMAYRS GGGFGVRLDA GNGFQGAVIT PYYDSLLVKL
410 420 430 440 450
STWALTFEQA ARKMLRNLRT FRIRVFKTNI PFLENVVQHP KFLSGEYDTS
460 470 480 490 500
FIDTTPELFV FPRRKDRGTK MLTYIGTVTV NGFPGIGKKK KPVFDKPRLP
510 520 530 540 550
KLSEAEPIPA GTKQILDKHG PEGLVRWIQE QPRVLLTDTT FRDAHQSLLA
560 570 580 590 600
TRVRTVDLVR AAEPSARLLP NLFSLEMWGG ATFDVAYRFL KEDPWDRLLK
610 620 630 640 650
LRDAFPNVLF QMLLRSANAV GYKNYPDNVI REFVEKSAHA GIHVFRIFDS
660 670 680 690 700
LNWVKGMTVA IDAVRQSGKI AEAAICYTGD ILDPSRSKYN LDYYKALAKE
710 720 730 740 750
LEQAGAHILA IKDMAGLLKP QAAHVLISGL KETVDIPIYL HTHDTSGNGI
760 770 780 790 800
YTYAKAIEAG VDIVDVAISS MAGLTSQPSA NTLYYALEGT ERAPEVDIYG
810 820 830 840 850
LEQLARYWED VRKFYQEFES GMNAPHTEVY MHEMPGGQYS NLQQQAKAVG
860 870 880 890 900
LGDRWDEVKE MYRRVNDLFG DIVKVTPSSK VVGDMALYMV QNNLTEQDIF
910 920 930 940 950
ERGETLNFPD SVVELFEGYL GQPHGGFPKE LQRIILKGRE PITVRPGELL
960 970 980 990 1000
EPVDFEQMKK ELYDKLGREV TDFDAIAYAL YPKVFLEYAE TVEKYGDVSV
1010 1020 1030 1040 1050
LDTPTFLYGM RLGEEIEVEI ERGKTLIVKL VSIGQPQADG TRVVYFELNG
1060 1070 1080 1090 1100
QPREVVIRDE SIKTAVVEHI KADRTNPNHI AATMPGTVVK VLVEKGEKVD
1110 1120 1130 1140
KGDHLMVTEA MKMETTVQAP FAGVVKDIYV KSGDAIQAGD LLMELSK
Length:1,147
Mass (Da):128,337
Last modified:May 1, 1997 - v1
Checksum:iDFD2F003F838F591
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D83706 Genomic DNA. Translation: BAA12072.1.

Similar proteinsi

Entry informationi

Entry nameiP94448_GEOSE
AccessioniPrimary (citable) accession number: P94448
Entry historyiIntegrated into UniProtKB/TrEMBL: May 1, 1997
Last sequence update: May 1, 1997
Last modified: June 7, 2017
This is version 90 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)