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Reviewed, UniProtKB/Swiss-Prot P94428 (GABD_BACSU)

Last modified November 3, 2009. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Succinate-semialdehyde dehydrogenase [NADP+]
      Short name=SSDH
    EC=1.2.1.16
Gene names
Name: gabD
Synonyms: ycnH
Ordered Locus Names: BSU03910
OrganismBacillus subtilis [Complete proteome] [HAMAP]
Taxonomic identifier1423 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

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Protein attributes

Sequence length462 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Catalytic activity

Succinate semialdehyde + NAD(P)+ + H2O = succinate + NAD(P)H.

Pathway

Amino-acid degradation; 4-aminobutanoate degradation.

Induction

Transcriptionally regulated by gabR in the presence of GABA. Up-regulated under low pH conditions and ethanol. Expression from the gabD promoter was induced by growth in the presence of glutamate. Ref.3 Ref.4

Disruption phenotype

Cells lacking this gene are unable to use GABA as sole nitrogen source. Ref.3

Miscellaneous

Expressed from the major gabT promoter and from an additional gabD promoter.

Sequence similarities

Belongs to the aldehyde dehydrogenase family.

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Ontologies

Keywords
   LigandNADP
   Molecular functionOxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processoxidation reduction

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionsuccinate-semialdehyde dehydrogenase [NAD(P)+] activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 462462Succinate-semialdehyde dehydrogenase [NADP+]
PRO_0000377373

Regions

Nucleotide binding210 – 2156NAD or NADP By similarity

Sites

Active site2321 By similarity
Active site2661 By similarity

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Sequences

Sequence LengthMass (Da)Tools
P94428-1 [UniParc].

Last modified May 1, 1997. Version 1.
Checksum: 56CFCFF6E0960110

FASTA46250,262
        10         20         30         40         50         60 
MPDQLTVYNP ATGEEIKTIP QQSATEVEEA IERSHQAFKT WSKTSANERT SLLKKWYELI 

        70         80         90        100        110        120 
VEHKEELADL ITKENGKPYQ EAVGEVLYGA GYIEWFAEEA KRVYGRTVPA PTTGKRIVVT 

       130        140        150        160        170        180 
RQPVGPVAAI TPWNFPNAMI TRKAAPALAA GCTFIIKPAP DTPLSAYELA RLAYEAGIPK 

       190        200        210        220        230        240 
DVLQVVIGDG EEIGNVFTSS PKIRKITFTG STPVGKILMK NSADTVKHVS MELGGHAPLI 

       250        260        270        280        290        300 
VDEDADIDLA VEQAMASKYR NAGQTCVCAN RLIVHESIKD EFAAKLSEQV SKLKVGNGLE 

       310        320        330        340        350        360 
EGVNVGPIIN KRGFEKIVSQ IDDAVEKGAK VIAGGTYDRN DDKGCYFVNP TVLTDVDTSM 

       370        380        390        400        410        420 
NIMHEETFGP VAPIVTFSDI DEAIQLANDT PYGLAAYFFT ENYRRGIYIS ENLEYGIIGW 

       430        440        450        460 
NDGGPSAVQA PFGGMKESGI GREGGSEGIE PYLETKYLSI GL

« Hide

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References

« Hide 'large scale' references
[1]"The 25 degrees-36 degrees region of the Bacillus subtilis chromosome: determination of the sequence of a 146 kb segment and identification of 113 genes."
Yamane K., Kumano M., Kurita K.
Microbiology 142:3047-3056(1996) [PubMed: 8969502] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[2]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed: 9384377] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[3]"GabR, a member of a novel protein family, regulates the utilization of gamma-aminobutyrate in Bacillus subtilis."
Belitsky B.R., Sonenshein A.L.
Mol. Microbiol. 45:569-583(2002) [PubMed: 12123465] [Abstract]
Cited for: FUNCTION IN THE UTILIZATION OF GABA, INDUCTION, DISRUPTION PHENOTYPE.
Strain: 168 / SMY.
[4]"Acid and base stress and transcriptomic responses in Bacillus subtilis."
Wilks J.C., Kitko R.D., Cleeton S.H., Lee G.E., Ugwu C.S., Jones B.D., BonDurant S.S., Slonczewski J.L.
Appl. Environ. Microbiol. 75:981-990(2009) [PubMed: 19114526] [Abstract]
Cited for: INDUCTION AT LOW PH.
Strain: 168 / JH642.

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Cross-references

Sequence databases

D50453 Genomic DNA. Translation: BAA09022.1.
AL009126 Genomic DNA. Translation: CAB12199.1.
PIRD69764.
RefSeqNP_388273.1.

3D structure databases

HSSPHSSP built from PDB template 1BXS based on UniProtKB P51977.
ModBaseSearch...

Genome annotation databases

GeneID939976.
GenomeReviewsGene locus BSU03910 in contig AL009126_GR.
KEGGbsu:BSU03910.
NMPDRfig|224308.1.peg.392.

Organism-specific databases

SubtiListBG12044. gabD. [Micado]
CMRSearch...

Phylogenomic databases

HOGENOMP94428.
OMAIADPATH.

Enzyme and pathway databases

BioCycBSUB224308:BSU0392-MON.

Family and domain databases

InterProIPR016160. Ald_DH_CS.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH.
IPR010102. Succ_semiAld_DH.
[Graphical view]
Gene3DG3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHERPTHR11699. Aldehyde_dehyd. 1 hit.
PfamPF00171. Aldedh. 1 hit.
[Graphical view]
TIGRFAMsTIGR01780. SSADH. 1 hit.
PROSITEPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. False negative.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameGABD_BACSU
AccessionPrimary (citable) accession number: P94428
Secondary accession number(s): Q797N5
Entry history
Integrated into UniProtKB/Swiss-Prot: June 16, 2009
Last sequence update: May 1, 1997
Last modified: November 3, 2009
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents