Reviewed, UniProtKB/Swiss-Prot P94417 (AK3_BACSU)

Names and origin

Protein names

Recommended name:
    Probable aspartokinase
    EC=2.7.2.4
Alternative name(s):
    Aspartate kinase

Gene names

Name:	yclM
Ordered Locus Names:	BSU03790

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	454 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-aspartate = ADP + 4-phospho-L-aspartate.
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; tetrahydrodipicolinate from L-aspartate: step 1/4. Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 1/3. Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 1/5.
Sequence similarities	Belongs to the aspartokinase family. Contains 2 ACT domains.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Diaminopimelate biosynthesis Lysine biosynthesis
Domain	Repeat
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	diaminopimelate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW amino acid binding Inferred from electronic annotation. Source: InterPro aspartate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 454	454	Probable aspartokinase		PRO_0000066672
Regions
	Domain	310 – 381	72	ACT 1
	Domain	385 – 454	70	ACT 2

Sequences

	Sequence		Length	Mass (Da)
	P94417-1 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: A9031ACB65A1D11C	FASTA	454	49,825
	10 20 30 40 50 60 MKVVKFGGSS LASGAQLDKV FHIVTSDPAR KAVVVSAPGK HYAEDTKVTD LLIACAEQYL 70 80 90 100 110 120 ATGSAPELAE AVVERYALIA NELQLGQSII EKIRDDLFTL LEGDKSNPEQ YLDAVKASGE 130 140 150 160 170 180 DNNAKLIAAY FRYKGVKAEY VNPKDAGLFV TNEPGNAQVL PESYQNLYRL RERDGLIIFP 190 200 210 220 230 240 GFFGFSKDGD VITFSRSGSD ITGSILANGL QADLYENFTD VDAVYSVNPS FVENPKEISE 250 260 270 280 290 300 LTYREMRELS YAGFSVFHDE ALIPAFRAGI PVQIKNTNNP SAEGTRVVSK RDNTNGPVVG 310 320 330 340 350 360 IASDTGFCSI YISKYLMNRE IGFGRRALQI LEEHGLTYEH VPSGIDDMTI ILRQGQMDAA 370 380 390 400 410 420 TERSVIKRIE EDLHADEVIV EHHLALIMVV GEAMRHNVGT TARAAKALSE AQVNIEMINQ 430 440 450 GSSEVSMMFG VKEAEERKAV QALYQEFFAG VLIS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The 25 degrees-36 degrees region of the Bacillus subtilis chromosome: determination of the sequence of a 146 kb segment and identification of 113 genes." Yamane K., Kumano M., Kurita K. Microbiology 142:3047-3056(1996) [PubMed: 8969502] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[2]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D50453 Genomic DNA. Translation: BAA09011.1. AL009126 Genomic DNA. Translation: CAB12187.1.
PIR	A69763.
RefSeq	NP_388261.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	938276.
GenomeReviews	Gene locus BSU03790 in contig AL009126_GR.
KEGG	bsu:BSU03790.
NMPDR	fig|224308.1.peg.380.
Organism-specific databases
SubtiList	BG12033. yclM. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	P94417.
OMA	P94417. YLMNREI.
Enzyme and pathway databases
BioCyc	BSUB224308:BSU0380-MON. MetaCyc:MON-6563.
BRENDA	2.7.2.4. 150.
Family and domain databases
InterPro	IPR002912. ACT_bd. IPR001048. Asp/Glu/Uridylate_kinase. IPR001341. Asp_kin_reg. IPR018042. Aspartate_kinase_CS. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit.
Pfam	PF00696. AA_kinase. 1 hit. PF01842. ACT. 2 hits. [Graphical view]
TIGRFAMs	TIGR00657. asp_kinases. 1 hit.
PROSITE	PS00324. ASPARTOKINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AK3_BACSU

Accession

Primary (citable) accession number: P94417

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1997
Last modified:	June 16, 2009
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)