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Protein

GTP cyclohydrolase FolE2

Gene

folE2

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Converts GTP to 7,8-dihydroneopterin triphosphate.1 Publication

Catalytic activityi

GTP + H2O = formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate.1 Publication

Pathway:i7,8-dihydroneopterin triphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 7,8-dihydroneopterin triphosphate from GTP.
Proteins known to be involved in this subpathway in this organism are:
  1. GTP cyclohydrolase 1 (mtrA), GTP cyclohydrolase FolE2 (folE2), GTP cyclohydrolase 1 (folE)
This subpathway is part of the pathway 7,8-dihydroneopterin triphosphate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 7,8-dihydroneopterin triphosphate from GTP, the pathway 7,8-dihydroneopterin triphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei183 – 1831May be catalytically importantBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BioCyciBSUB:BSU03340-MONOMER.
UniPathwayiUPA00848; UER00151.

Protein family/group databases

TCDBi9.B.10.1.1. the putative tripartite zn(2+) transporter (tzt) family.

Names & Taxonomyi

Protein namesi
Recommended name:
GTP cyclohydrolase FolE2 (EC:3.5.4.16)
Alternative name(s):
GTP cyclohydrolase 1B
Gene namesi
Name:folE2
Synonyms:yciA
Ordered Locus Names:BSU03340
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001570 Componenti: Chromosome

Organism-specific databases

GenoListiBSU03340. [Micado]

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 304304GTP cyclohydrolase FolE2PRO_0000147701Add
BLAST

Proteomic databases

PaxDbiP94398.

Expressioni

Inductioni

Repressed by zinc, via zur.1 Publication

Interactioni

Protein-protein interaction databases

IntActiP94398. 1 interaction.
MINTiMINT-8366745.

Structurei

3D structure databases

ProteinModelPortaliP94398.
SMRiP94398. Positions 44-298.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GTP cyclohydrolase IV family.Curated

Phylogenomic databases

eggNOGiCOG1469.
HOGENOMiHOG000280679.
InParanoidiP94398.
KOiK09007.
OMAiGAHNQRG.
OrthoDBiEOG6X6RBH.
PhylomeDBiP94398.

Family and domain databases

HAMAPiMF_01527_B. GTP_cyclohydrol_B.
InterProiIPR022838. GTP_cyclohydrolase_FolE2.
IPR003801. GTP_cyclohydrolase_FolE2/MptA.
[Graphical view]
PfamiPF02649. GCHY-1. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00294. TIGR00294. 1 hit.

Sequencei

Sequence statusi: Complete.

P94398-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNQHTLLPKK TERLQYFGSV SPIKGEKPVE KEKMKDLQNI RKDYFFDIQH
60 70 80 90 100
VGVANVSHPV TITSAMMPAE QTTAANFTMT CNLPRNQKGI NMSRLTELLQ
110 120 130 140 150
VYHQNGWILS FSSLQQFTKE LAENMDTSSA TVEVRFPWFF ERKSPKLEKA
160 170 180 190 200
GLMHADIFMS VTYRKDQPFK QRAGISAKVT TLCPCSKEIS EYSAHNQRGT
210 220 230 240 250
VSIWADIHPA ASLPSDVKAD LLHAAESNAS ARLHPVLKRP DEKAVTETAY
260 270 280 290 300
ENPRFVEDLA RLIAADLFEL EWVSAFEIEC RNEESIHLHD AYAKLCFSKE

VDKI
Length:304
Mass (Da):34,564
Last modified:June 16, 2009 - v2
Checksum:i90A28F90FE9BDAB3
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti202 – 2098SIWADIHP → KHLGRIFTR in BAA08968 (PubMed:8969502).Curated
Sequence conflicti222 – 2221L → P in BAA08968 (PubMed:8969502).Curated
Sequence conflicti290 – 2956DAYAKL → RCLCEV in BAA08968 (PubMed:8969502).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50453 Genomic DNA. Translation: BAA08968.1.
AL009126 Genomic DNA. Translation: CAB12128.2.
PIRiH69759.
RefSeqiNP_388216.2. NC_000964.3.
WP_003246378.1. NZ_JNCM01000030.1.

Genome annotation databases

EnsemblBacteriaiCAB12128; CAB12128; BSU03340.
GeneIDi938325.
KEGGibsu:BSU03340.
PATRICi18972227. VBIBacSub10457_0342.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50453 Genomic DNA. Translation: BAA08968.1.
AL009126 Genomic DNA. Translation: CAB12128.2.
PIRiH69759.
RefSeqiNP_388216.2. NC_000964.3.
WP_003246378.1. NZ_JNCM01000030.1.

3D structure databases

ProteinModelPortaliP94398.
SMRiP94398. Positions 44-298.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP94398. 1 interaction.
MINTiMINT-8366745.

Protein family/group databases

TCDBi9.B.10.1.1. the putative tripartite zn(2+) transporter (tzt) family.

Proteomic databases

PaxDbiP94398.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB12128; CAB12128; BSU03340.
GeneIDi938325.
KEGGibsu:BSU03340.
PATRICi18972227. VBIBacSub10457_0342.

Organism-specific databases

GenoListiBSU03340. [Micado]

Phylogenomic databases

eggNOGiCOG1469.
HOGENOMiHOG000280679.
InParanoidiP94398.
KOiK09007.
OMAiGAHNQRG.
OrthoDBiEOG6X6RBH.
PhylomeDBiP94398.

Enzyme and pathway databases

UniPathwayiUPA00848; UER00151.
BioCyciBSUB:BSU03340-MONOMER.

Family and domain databases

HAMAPiMF_01527_B. GTP_cyclohydrol_B.
InterProiIPR022838. GTP_cyclohydrolase_FolE2.
IPR003801. GTP_cyclohydrolase_FolE2/MptA.
[Graphical view]
PfamiPF02649. GCHY-1. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00294. TIGR00294. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The 25 degrees-36 degrees region of the Bacillus subtilis chromosome: determination of the sequence of a 146 kb segment and identification of 113 genes."
    Yamane K., Kumano M., Kurita K.
    Microbiology 142:3047-3056(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168.
  2. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.
  3. "From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later."
    Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.
    Microbiology 155:1758-1775(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: SEQUENCE REVISION TO 202-209; 222 AND 290-295.
  4. "Functional analysis of the Bacillus subtilis Zur regulon."
    Gaballa A., Wang T., Ye R.W., Helmann J.D.
    J. Bacteriol. 184:6508-6514(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
    Strain: 168.
  5. Cited for: FUNCTION, CATALYTIC ACTIVITY.

Entry informationi

Entry nameiGCH4_BACSU
AccessioniPrimary (citable) accession number: P94398
Secondary accession number(s): Q797Q1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: June 16, 2009
Last modified: June 24, 2015
This is version 87 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Was originally thought to be a zinc uptake system.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.