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P94391 (ROCA2_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1-pyrroline-5-carboxylate dehydrogenase 2
Short name=P5C dehydrogenase 2
EC=1.5.1.12
Gene names
Name:ycgN
Ordered Locus Names:BSU03210
OrganismBacillus subtilis
Taxonomic identifier1423 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length515 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

(S)-1-pyrroline-5-carboxylate + NAD(P)+ + 2 H2O = L-glutamate + NAD(P)H. HAMAP MF_00733

Pathway

Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 2/2. HAMAP MF_00733

Sequence similarities

Belongs to the aldehyde dehydrogenase family. RocA subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 5155151-pyrroline-5-carboxylate dehydrogenase 2 HAMAP MF_00733
PRO_0000056512

Sites

Active site2861 By similarity
Active site3201 By similarity

Experimental info

Sequence conflict181N → Y in BAA08955. Ref.1
Sequence conflict2391A → S in BAA08955. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P94391 [UniParc].

Last modified June 16, 2009. Version 2.
Checksum: 937CAAFF1F97A009

FASTA51556,424
        10         20         30         40         50         60 
MTTPYKHEPF TNFQDQNNVE AFKKALATVS EYLGKDYPLV INGERVETEA KIVSINPADK 

        70         80         90        100        110        120 
EEVVGRVSKA SQEHAEQAIQ AAAKAFEEWR YTSPEERAAV LFRAAAKVRR RKHEFSALLV 

       130        140        150        160        170        180 
KEAGKPWNEA DADTAEAIDF MEYYARQMIE LAKGKPVNSR EGEKNQYVYT PTGVTVVIPP 

       190        200        210        220        230        240 
WNFLFAIMAG TTVAPIVTGN TVVLKPASAT PVIAAKFVEV LEESGLPKGV VNFVPGSGAE 

       250        260        270        280        290        300 
VGDYLVDHPK TSLITFTGSR EVGTRIFERA AKVQPGQQHL KRVIAEMGGK DTVVVDEDAD 

       310        320        330        340        350        360 
IELAAQSIFT SAFGFAGQKC SAGSRAVVHE KVYDQVLERV IEITESKVTA KPDSADVYMG 

       370        380        390        400        410        420 
PVIDQGSYDK IMSYIEIGKQ EGRLVSGGTG DDSKGYFIKP TIFADLDPKA RLMQEEIFGP 

       430        440        450        460        470        480 
VVAFCKVSDF DEALEVANNT EYGLTGAVIT NNRKHIERAK QEFHVGNLYF NRNCTGAIVG 

       490        500        510 
YHPFGGFKMS GTDSKAGGPD YLALHMQAKT ISEMF

« Hide

References

« Hide 'large scale' references
[1]"The 25 degrees-36 degrees region of the Bacillus subtilis chromosome: determination of the sequence of a 146 kb segment and identification of 113 genes."
Yamane K., Kumano M., Kurita K.
Microbiology 142:3047-3056(1996) [PubMed: 8969502] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[2]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed: 9384377] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[3]"From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later."
Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.
Microbiology 155:1758-1775(2009) [PubMed: 19383706] [Abstract]
Cited for: SEQUENCE REVISION TO 18 AND 239.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		D50453 Genomic DNA. Translation: BAA08955.1.
AL009126 Genomic DNA. Translation: CAB12115.2.
PIRA69759.
RefSeqNP_388203.2. NC_000964.3.

3D structure databases

ProteinModelPortalP94391.
SMRP94391. Positions 1-515.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBBACT00000003974; EBBACP00000003974; EBBACG00000003966.
GeneID938333.
GenomeReviewsGene locus BSU03210 in contig AL009126_GR.
KEGGbsu:BSU03210.
NMPDRfig|224308.1.peg.322.
PATRIC18972201. VBIBacSub10457_0329.

Organism-specific databases

GenoListBSU03210.

Phylogenomic databases

GeneTreeEBGT00070000031871.
HOGENOMHBG752218.
PhylomeDBP94391.
ProtClustDBPRK03137.

Enzyme and pathway databases

BioCycBSUB:BSU03210-MONOMER.

Family and domain databases

HAMAPMF_00733. RocA.
[Tree]
InterProIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR005932. Delta1-pyrroline-5-COlate_DH-2.
[Graphical view]
Gene3DG3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit.
G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
KOK00294.
PfamPF00171. Aldedh. 1 hit.
[Graphical view]
SUPFAMSSF53720. Aldehyde_DH/Histidinol_DH. 1 hit.
TIGRFAMsTIGR01237. D1pyr5carbox2. 1 hit.
PROSITEPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameROCA2_BACSU
AccessionPrimary (citable) accession number: P94391
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2003
Last sequence update: June 16, 2009
Last modified: January 25, 2012
This is version 79 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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