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Protein

1-pyrroline-5-carboxylate dehydrogenase 2

Gene

putC

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Important for the use of proline as a sole carbon and energy source or a sole nitrogen source.1 Publication

Catalytic activityi

L-glutamate 5-semialdehyde + NAD+ + H2O = L-glutamate + NADH.UniRule annotation

Pathwayi: L-proline degradation into L-glutamate

This protein is involved in step 2 of the subpathway that synthesizes L-glutamate from L-proline.UniRule annotation1 Publication
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Proline dehydrogenase 1 (fadM), Proline dehydrogenase 2 (putB)
  2. 1-pyrroline-5-carboxylate dehydrogenase (rocA), 1-pyrroline-5-carboxylate dehydrogenase 2 (putC)
This subpathway is part of the pathway L-proline degradation into L-glutamate, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate from L-proline, the pathway L-proline degradation into L-glutamate and in Amino-acid degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei286UniRule annotation1
Active sitei320UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
LigandNAD

Enzyme and pathway databases

BioCyciBSUB:BSU03210-MONOMER
UniPathwayiUPA00261; UER00374

Names & Taxonomyi

Protein namesi
Recommended name:
1-pyrroline-5-carboxylate dehydrogenase 2Curated (EC:1.2.1.88UniRule annotation)
Short name:
P5C dehydrogenase 2Curated
Alternative name(s):
L-glutamate gamma-semialdehyde dehydrogenaseUniRule annotation
Gene namesi
Name:putC1 Publication
Synonyms:ycgN
Ordered Locus Names:BSU03210
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Pathology & Biotechi

Disruption phenotypei

Deletion of the putBCP operon abolishes L-proline utilization.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000565121 – 5151-pyrroline-5-carboxylate dehydrogenase 2Add BLAST515

Proteomic databases

PaxDbiP94391
PRIDEiP94391

Expressioni

Inductioni

The expression of the putBCP operon is induced in a PutR-dependent fashion by very low concentrations of L-proline in the growth medium. CodY represses the operon by displacing PutR from DNA.3 Publications

Interactioni

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100001793

Structurei

3D structure databases

ProteinModelPortaliP94391
SMRiP94391
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the aldehyde dehydrogenase family. RocA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C26 Bacteria
COG1012 LUCA
HOGENOMiHOG000271511
InParanoidiP94391
KOiK00294
OMAiNQPTTGM
PhylomeDBiP94391

Family and domain databases

CDDicd07124 ALDH_PutA-P5CDH-RocA, 1 hit
Gene3Di3.40.309.10, 1 hit
3.40.605.10, 2 hits
HAMAPiMF_00733 RocA, 1 hit
InterProiView protein in InterPro
IPR016161 Ald_DH/histidinol_DH
IPR016163 Ald_DH_C
IPR016160 Ald_DH_CS_CYS
IPR029510 Ald_DH_CS_GLU
IPR016162 Ald_DH_N
IPR015590 Aldehyde_DH_dom
IPR005932 RocA
PfamiView protein in Pfam
PF00171 Aldedh, 1 hit
SUPFAMiSSF53720 SSF53720, 1 hit
TIGRFAMsiTIGR01237 D1pyr5carbox2, 1 hit
PROSITEiView protein in PROSITE
PS00070 ALDEHYDE_DEHYDR_CYS, 1 hit
PS00687 ALDEHYDE_DEHYDR_GLU, 1 hit

Sequencei

Sequence statusi: Complete.

P94391-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTPYKHEPF TNFQDQNNVE AFKKALATVS EYLGKDYPLV INGERVETEA
60 70 80 90 100
KIVSINPADK EEVVGRVSKA SQEHAEQAIQ AAAKAFEEWR YTSPEERAAV
110 120 130 140 150
LFRAAAKVRR RKHEFSALLV KEAGKPWNEA DADTAEAIDF MEYYARQMIE
160 170 180 190 200
LAKGKPVNSR EGEKNQYVYT PTGVTVVIPP WNFLFAIMAG TTVAPIVTGN
210 220 230 240 250
TVVLKPASAT PVIAAKFVEV LEESGLPKGV VNFVPGSGAE VGDYLVDHPK
260 270 280 290 300
TSLITFTGSR EVGTRIFERA AKVQPGQQHL KRVIAEMGGK DTVVVDEDAD
310 320 330 340 350
IELAAQSIFT SAFGFAGQKC SAGSRAVVHE KVYDQVLERV IEITESKVTA
360 370 380 390 400
KPDSADVYMG PVIDQGSYDK IMSYIEIGKQ EGRLVSGGTG DDSKGYFIKP
410 420 430 440 450
TIFADLDPKA RLMQEEIFGP VVAFCKVSDF DEALEVANNT EYGLTGAVIT
460 470 480 490 500
NNRKHIERAK QEFHVGNLYF NRNCTGAIVG YHPFGGFKMS GTDSKAGGPD
510
YLALHMQAKT ISEMF
Length:515
Mass (Da):56,424
Last modified:June 16, 2009 - v2
Checksum:i937CAAFF1F97A009
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti18N → Y in BAA08955 (PubMed:8969502).Curated1
Sequence conflicti239A → S in BAA08955 (PubMed:8969502).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50453 Genomic DNA Translation: BAA08955.1
AL009126 Genomic DNA Translation: CAB12115.2
PIRiA69759
RefSeqiNP_388203.2, NC_000964.3
WP_003234655.1, NZ_JNCM01000030.1

Genome annotation databases

EnsemblBacteriaiCAB12115; CAB12115; BSU03210
GeneIDi938333
KEGGibsu:BSU03210
PATRICifig|224308.179.peg.335

Similar proteinsi

Entry informationi

Entry nameiROCA2_BACSU
AccessioniPrimary (citable) accession number: P94391
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 28, 2003
Last sequence update: June 16, 2009
Last modified: May 23, 2018
This is version 117 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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