Reviewed, UniProtKB/Swiss-Prot P94390 (PROD2_BACSU)

Names and origin

Protein names

Recommended name:
    Proline dehydrogenase 2
      Short name=PRODH 2
    EC=1.5.99.8
Alternative name(s):
    Proline oxidase 2

Gene names

Name:	ycgM
Ordered Locus Names:	BSU03200

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	303 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidation of L-proline to delta-1-pyrroline-5-carboxylate (P5C) By similarity.
Catalytic activity	L-proline + acceptor = (S)-1-pyrroline-5-carboxylate + reduced acceptor.
Cofactor	FAD By similarity.
Pathway	Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 1/2.
Sequence similarities	Belongs to the proline dehydrogenase family.

Ontologies

Keywords
Biological process	Proline metabolism
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamate biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW proline catabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	proline dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 303	303	Proline dehydrogenase 2		PRO_0000361669

Sequences

	Sequence		Length	Mass (Da)
	P94390-1 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: D2FBAD6E05947B97	FASTA	303	35,046
	10 20 30 40 50 60 MITRDFFLFL SKSGFLNKMA RNWGSRVAAG KIIGGNDFNS SIPTIRQLNS QGLSVTVDHL 70 80 90 100 110 120 GEFVNSAEVA RERTEECIQT IATIADQELN SHVSLKMTSL GLDIDMDLVY ENMTKILQTA 130 140 150 160 170 180 EKHKIMVTID MEDEVRCQKT LDIFKDFRKK YEHVSTVLQA YLYRTEKDID DLDSLNPFLR 190 200 210 220 230 240 LVKGAYKESE KVAFPEKSDV DENYKKIIRK QLLNGHYTAI ATHDDKMIDF TKQLAKEHGI 250 260 270 280 290 300 ANDKFEFQML YGMRSQTQLS LVKEGYNMRV YLPYGEDWYG YFMRRLAERP SNIAFAFKGM TKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The 25 degrees-36 degrees region of the Bacillus subtilis chromosome: determination of the sequence of a 146 kb segment and identification of 113 genes." Yamane K., Kumano M., Kurita K. Microbiology 142:3047-3056(1996) [PubMed: 8969502] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[2]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D50453 Genomic DNA. Translation: BAA08954.1. AL009126 Genomic DNA. Translation: CAB12114.1.
PIR	H69758.
RefSeq	NP_388202.1.
3D structure databases
SMR	P94390. Positions 3-289.
ModBase	Search...
Genome annotation databases
GeneID	938338.
GenomeReviews	Gene locus BSU03200 in contig AL009126_GR.
KEGG	bsu:BSU03200.
Organism-specific databases
SubtiList	BG12011. ycgM. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	HBG631970.
OMA	QWYGYFM.
Enzyme and pathway databases
BioCyc	SUBTI:BSU03200-MONOMER.
Family and domain databases
InterPro	IPR015590. Aldehyde_DH. IPR002872. Proline_DH. IPR008219. Proline_DH_prd. [Graphical view]
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit.
Pfam	PF01619. Pro_dh. 1 hit. [Graphical view]
PIRSF	PIRSF000196. Pro_dehydrog. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PROD2_BACSU

Accession

Primary (citable) accession number: P94390
Secondary accession number(s): Q797Q5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 10, 2009
Last sequence update:	May 1, 1997
Last modified:	January 19, 2010
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)