P94370 (SIGY_BACSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA polymerase sigma factor SigY

Gene names

Name:	sigY
Synonyms:	yxlB
Ordered Locus Names:	BSU38700

Organism

Bacillus subtilis (strain 168) [Reference proteome] [HAMAP]

Taxonomic identifier

224308 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus subtilis group › Bacillus subtilis › Bacillus subtilis subsp. subtilis

Protein attributes

Sequence length	178 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. Positively regulates the expression of the sigY-yxlCDEFG operon upon nitrogen starvation. Also positively regulates ybgB. Ref.3
Induction	Positively autoregulated. Induced upon nitrogen starvation. Ref.3 Ref.4
Miscellaneous	Negatively regulated by both YxlD and YxlE.
Sequence similarities	Belongs to the sigma-70 factor family. ECF subfamily.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Ligand	DNA-binding
Molecular function	Sigma factor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA-dependent transcription, initiation Inferred from electronic annotation. Source: InterPro
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro sigma factor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 178	178	RNA polymerase sigma factor SigY		PRO_0000094018
Regions
	DNA binding	146 – 165	20	H-T-H motif By similarity
	Motif	48 – 61	14	Polymerase core binding Potential

Sequences

	Sequence		Length	Mass (Da)
	P94370 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 2D1F42C3DDEABC6A	FASTA	178	21,358
	10 20 30 40 50 60 MDTQEEQRLI QQAKEGNDEA FTALFHYHYS FLYKYLLKLS LHPDLSEELV QETFLKGYIH 70 80 90 100 110 120 LRSFQGRSKF STWLISIASR LYLDHQKKRK REWKRNQTVT EETIRKIKWD VSAKGAEWSE 130 140 150 160 170 TLDLFSKLDP KLRTPVLLRH YYGYTYAEIG VMLQIKEGTV KSRVHKGLQQ IRKEWDDE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing of a 65 kb region of the Bacillus subtilis genome containing the lic and cel loci, and creation of a 177 kb contig covering the gnt-sacXY region." Yoshida K., Shindo K., Sano H., Seki S., Fujimura M., Yanai N., Miwa Y., Fujita Y. Microbiology 142:3113-3123(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168 / BGSC1A1.
[2]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[3]	"Regulation of the Bacillus subtilis extracytoplasmic function protein sigma(Y) and its target promoters." Cao M., Salzberg L., Tsai C.S., Mascher T., Bonilla C., Wang T., Ye R.W., Marquez-Magana L., Helmann J.D. J. Bacteriol. 185:4883-4890(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION AS A REGULATOR, INDUCTION, REGULATION BY YXLD AND YXLE. Strain: 168 / CU1065.
[4]	"Organization and expression of the Bacillus subtilis sigY operon." Tojo S., Matsunaga M., Matsumoto T., Kang C.-M., Yamaguchi H., Asai K., Sadaie Y., Yoshida K., Fujita Y. J. Biochem. 134:935-946(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION BY NITROGEN STARVATION. Strain: 168.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D83026 Genomic DNA. Translation: BAA11733.1. AL009126 Genomic DNA. Translation: CAB15896.1.
PIR	B69707.
RefSeq	NP_391749.1. NC_000964.3.
3D structure databases
ProteinModelPortal	P94370.
SMR	P94370. Positions 2-177.
ModBase	Search...
Protein-protein interaction databases
IntAct	P94370. 1 interaction.
STRING	224308.BSU38700.
Proteomic databases
PaxDb	P94370.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAB15896; CAB15896; BSU38700.
GeneID	937400.
KEGG	bsu:BSU38700.
PATRIC	18979788. VBIBacSub10457_4056.
Organism-specific databases
GenoList	BSU38700. [Micado]
Phylogenomic databases
eggNOG	COG1595.
HOGENOM	HOG000094755.
KO	K03088.
OMA	HYYGYTY.
ProtClustDB	PRK09638.
Enzyme and pathway databases
BioCyc	BSUB:BSU38700-MONOMER.
Family and domain databases
Gene3D	1.10.10.10. 1 hit.
InterPro	IPR014284. RNA_pol_sigma-70_dom. IPR000838. RNA_pol_sigma70_ECF_CS. IPR007627. RNA_pol_sigma70_r2. IPR007630. RNA_pol_sigma70_r4. IPR013325. RNA_pol_sigma_r2. IPR013324. RNA_pol_sigma_r3_r4. IPR011991. WHTH_DNA-bd_dom. [Graphical view]
Pfam	PF04542. Sigma70_r2. 1 hit. PF04545. Sigma70_r4. 1 hit. [Graphical view]
SUPFAM	SSF88946. Sigma_r2. 1 hit. SSF88659. Sigma_r3_r4. 1 hit.
TIGRFAMs	TIGR02937. sigma70-ECF. 1 hit.
PROSITE	PS01063. SIGMA70_ECF. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SIGY_BACSU

Accession

Primary (citable) accession number: P94370

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	May 1, 1997
Last modified:	May 1, 2013
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program