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P94325 (CISY_BRADU) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Citrate synthase

EC=2.3.3.16
Gene names
Name:gltA
Ordered Locus Names:blr4839
OrganismBradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110) [Reference proteome] [HAMAP]
Taxonomic identifier224911 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium

Protein attributes

Sequence length434 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Acetyl-CoA + H2O + oxaloacetate = citrate + CoA.

Pathway

Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 1/2.

Sequence similarities

Belongs to the citrate synthase family.

Ontologies

Keywords
   Biological processTricarboxylic acid cycle
   Molecular functionTransferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcellular carbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

tricarboxylic acid cycle

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: InterPro

   Molecular_functioncitrate (Si)-synthase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 434434Citrate synthase
PRO_0000169939

Sites

Active site3101 By similarity
Active site3681 By similarity

Experimental info

Sequence conflict2101P → S in AAB39736. Ref.1
Sequence conflict2811A → G in AAB39736. Ref.1
Sequence conflict3461M → L in AAB39736. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P94325 [UniParc].

Last modified February 28, 2003. Version 2.
Checksum: DEB33EC2B4BACDB4

FASTA43448,193
        10         20         30         40         50         60 
MDAKASNKTA TLTVGNKNYD LPIHSGSVGP DVIDIGKLYG QSGLFTYDPG FTSTASCQSK 

        70         80         90        100        110        120 
ITYIDGDAGV LEYRGYPIEQ LAENGDFLET CYLLLYGNLP TAAQKKDFDD RVIHHTMVHE 

       130        140        150        160        170        180 
QMARFFQGFR RDAHPMAVMV ASVGALAAFY HDSTDINDPK QRMIASMRMI AKIPTLAAMA 

       190        200        210        220        230        240 
YKYTIGQPFV YPKNSLKFAE NFLHMCFAVP CEEYKINPVL ADALDKIFIL HADHEQNAST 

       250        260        270        280        290        300 
STVRIAGSSG ANPFACIAAG IACLWGPAHG GANEAALAML AEIGSVDKIP EFIAKVKDKN 

       310        320        330        340        350        360 
SEVRLMGFGH RVYKNYDPRA KIMQKMCHAV LKETGHGDDP MLKVAMELEK IALSDQYFID 

       370        380        390        400        410        420 
RKLYPNVDFY SGITLKAMGF PVSMFTVLFA VARTVGWISQ WSEMIEDPQQ KIGRPRQLYT 

       430 
GVTRRDYVAI KDRK 

« Hide

References

« Hide 'large scale' references
[1]"Cloning of the Bradyrhizobium japonicum gltA gene encoding a central enzyme of the TCA cycle."
Levier K., Guerinot M.L.
Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 61A152.
[2]"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110."
Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S.
DNA Res. 9:189-197(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U76375 Genomic DNA. Translation: AAB39736.1.
BA000040 Genomic DNA. Translation: BAC50104.1.
RefSeqNP_771479.1. NC_004463.1.

3D structure databases

ProteinModelPortalP94325.
SMRP94325. Positions 9-433.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224911.blr4839.

Proteomic databases

PRIDEP94325.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAC50104; BAC50104; BAC50104.
GeneID1052140.
KEGGbja:blr4839.
PATRIC21193340. VBIBraJap65052_4919.

Phylogenomic databases

eggNOGCOG0372.
HOGENOMHOG000021224.
KOK01647.
OrthoDBEOG6P8TP4.
PhylomeDBP94325.

Enzyme and pathway databases

BioCycBJAP224911:GJEJ-4872-MONOMER.
UniPathwayUPA00223; UER00717.

Family and domain databases

Gene3D1.10.230.10. 1 hit.
1.10.580.10. 1 hit.
InterProIPR016142. Citrate_synth-like_lrg_a-sub.
IPR016143. Citrate_synth-like_sm_a-sub.
IPR002020. Citrate_synthase-like.
IPR016141. Citrate_synthase-like_core.
IPR019810. Citrate_synthase_AS.
IPR024176. Citrate_synthase_bac-typ.
IPR010953. Citrate_synthase_typ-II.
[Graphical view]
PANTHERPTHR11739. PTHR11739. 1 hit.
PfamPF00285. Citrate_synt. 1 hit.
[Graphical view]
PIRSFPIRSF001369. Citrate_synth. 1 hit.
PRINTSPR00143. CITRTSNTHASE.
SUPFAMSSF48256. SSF48256. 1 hit.
TIGRFAMsTIGR01798. cit_synth_I. 1 hit.
PROSITEPS00480. CITRATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCISY_BRADU
AccessionPrimary (citable) accession number: P94325
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: February 28, 2003
Last modified: July 9, 2014
This is version 84 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways