Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Citrate synthase

Gene

gltA

Organism
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Acetyl-CoA + H2O + oxaloacetate = citrate + CoA.PROSITE-ProRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei310 – 3101PROSITE-ProRule annotation
Active sitei368 – 3681PROSITE-ProRule annotation

GO - Molecular functioni

  1. citrate (Si)-synthase activity Source: InterPro

GO - Biological processi

  1. cellular carbohydrate metabolic process Source: InterPro
  2. tricarboxylic acid cycle Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Tricarboxylic acid cycle

Enzyme and pathway databases

BioCyciBJAP224911:GJEJ-4872-MONOMER.
UniPathwayiUPA00223; UER00717.

Names & Taxonomyi

Protein namesi
Recommended name:
Citrate synthase (EC:2.3.3.16)
Gene namesi
Name:gltA
Ordered Locus Names:blr4839
OrganismiBradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Taxonomic identifieri224911 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
ProteomesiUP000002526 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 434434Citrate synthasePRO_0000169939Add
BLAST

Proteomic databases

PRIDEiP94325.

Interactioni

Protein-protein interaction databases

STRINGi224911.blr4839.

Structurei

3D structure databases

ProteinModelPortaliP94325.
SMRiP94325. Positions 9-433.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the citrate synthase family.Curated

Phylogenomic databases

eggNOGiCOG0372.
HOGENOMiHOG000021224.
InParanoidiP94325.
KOiK01647.
OrthoDBiEOG6P8TP4.
PhylomeDBiP94325.

Family and domain databases

Gene3Di1.10.230.10. 1 hit.
1.10.580.10. 1 hit.
InterProiIPR016142. Citrate_synth-like_lrg_a-sub.
IPR016143. Citrate_synth-like_sm_a-sub.
IPR002020. Citrate_synthase-like.
IPR016141. Citrate_synthase-like_core.
IPR019810. Citrate_synthase_AS.
IPR024176. Citrate_synthase_bac-typ.
IPR010953. Citrate_synthase_typ-I.
[Graphical view]
PANTHERiPTHR11739. PTHR11739. 1 hit.
PfamiPF00285. Citrate_synt. 1 hit.
[Graphical view]
PIRSFiPIRSF001369. Citrate_synth. 1 hit.
PRINTSiPR00143. CITRTSNTHASE.
SUPFAMiSSF48256. SSF48256. 1 hit.
TIGRFAMsiTIGR01798. cit_synth_I. 1 hit.
PROSITEiPS00480. CITRATE_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P94325-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDAKASNKTA TLTVGNKNYD LPIHSGSVGP DVIDIGKLYG QSGLFTYDPG
60 70 80 90 100
FTSTASCQSK ITYIDGDAGV LEYRGYPIEQ LAENGDFLET CYLLLYGNLP
110 120 130 140 150
TAAQKKDFDD RVIHHTMVHE QMARFFQGFR RDAHPMAVMV ASVGALAAFY
160 170 180 190 200
HDSTDINDPK QRMIASMRMI AKIPTLAAMA YKYTIGQPFV YPKNSLKFAE
210 220 230 240 250
NFLHMCFAVP CEEYKINPVL ADALDKIFIL HADHEQNAST STVRIAGSSG
260 270 280 290 300
ANPFACIAAG IACLWGPAHG GANEAALAML AEIGSVDKIP EFIAKVKDKN
310 320 330 340 350
SEVRLMGFGH RVYKNYDPRA KIMQKMCHAV LKETGHGDDP MLKVAMELEK
360 370 380 390 400
IALSDQYFID RKLYPNVDFY SGITLKAMGF PVSMFTVLFA VARTVGWISQ
410 420 430
WSEMIEDPQQ KIGRPRQLYT GVTRRDYVAI KDRK
Length:434
Mass (Da):48,193
Last modified:February 27, 2003 - v2
Checksum:iDEB33EC2B4BACDB4
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti210 – 2101P → S in AAB39736 (Ref. 1) Curated
Sequence conflicti281 – 2811A → G in AAB39736 (Ref. 1) Curated
Sequence conflicti346 – 3461M → L in AAB39736 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U76375 Genomic DNA. Translation: AAB39736.1.
BA000040 Genomic DNA. Translation: BAC50104.1.
RefSeqiNP_771479.1. NC_004463.1.

Genome annotation databases

EnsemblBacteriaiBAC50104; BAC50104; BAC50104.
GeneIDi1052140.
KEGGibja:blr4839.
PATRICi21193340. VBIBraJap65052_4919.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U76375 Genomic DNA. Translation: AAB39736.1.
BA000040 Genomic DNA. Translation: BAC50104.1.
RefSeqiNP_771479.1. NC_004463.1.

3D structure databases

ProteinModelPortaliP94325.
SMRiP94325. Positions 9-433.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224911.blr4839.

Proteomic databases

PRIDEiP94325.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAC50104; BAC50104; BAC50104.
GeneIDi1052140.
KEGGibja:blr4839.
PATRICi21193340. VBIBraJap65052_4919.

Phylogenomic databases

eggNOGiCOG0372.
HOGENOMiHOG000021224.
InParanoidiP94325.
KOiK01647.
OrthoDBiEOG6P8TP4.
PhylomeDBiP94325.

Enzyme and pathway databases

UniPathwayiUPA00223; UER00717.
BioCyciBJAP224911:GJEJ-4872-MONOMER.

Family and domain databases

Gene3Di1.10.230.10. 1 hit.
1.10.580.10. 1 hit.
InterProiIPR016142. Citrate_synth-like_lrg_a-sub.
IPR016143. Citrate_synth-like_sm_a-sub.
IPR002020. Citrate_synthase-like.
IPR016141. Citrate_synthase-like_core.
IPR019810. Citrate_synthase_AS.
IPR024176. Citrate_synthase_bac-typ.
IPR010953. Citrate_synthase_typ-I.
[Graphical view]
PANTHERiPTHR11739. PTHR11739. 1 hit.
PfamiPF00285. Citrate_synt. 1 hit.
[Graphical view]
PIRSFiPIRSF001369. Citrate_synth. 1 hit.
PRINTSiPR00143. CITRTSNTHASE.
SUPFAMiSSF48256. SSF48256. 1 hit.
TIGRFAMsiTIGR01798. cit_synth_I. 1 hit.
PROSITEiPS00480. CITRATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of the Bradyrhizobium japonicum gltA gene encoding a central enzyme of the TCA cycle."
    Levier K., Guerinot M.L.
    Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 61A152.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110.

Entry informationi

Entry nameiCISY_BRADU
AccessioniPrimary (citable) accession number: P94325
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2000
Last sequence update: February 27, 2003
Last modified: January 6, 2015
This is version 88 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.