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P94323

- NODM_BRADU

UniProt

P94323 - NODM_BRADU

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Protein
Glutamine--fructose-6-phosphate aminotransferase [isomerizing]
Gene
nodM, blr1632
Organism
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Involved in the production of the root hair deformation (HAD) factor specifically on soybean By similarity.UniRule annotation

Catalytic activityi

L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21Nucleophile; for GATase activity By similarity
Active sitei603 – 6031For Fru-6P isomerization activity By similarity

GO - Molecular functioni

  1. carbohydrate binding Source: InterPro
  2. glutamine-fructose-6-phosphate transaminase (isomerizing) activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. carbohydrate biosynthetic process Source: InterPro
  2. glutamine metabolic process Source: UniProtKB-HAMAP
  3. nodulation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Keywords - Biological processi

Nodulation

Enzyme and pathway databases

BioCyciBJAP224911:GJEJ-1651-MONOMER.

Protein family/group databases

MEROPSiC44.971.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine--fructose-6-phosphate aminotransferase [isomerizing] (EC:2.6.1.16)
Short name:
GFAT
Alternative name(s):
Nodulation protein M
Gene namesi
Name:nodM
Ordered Locus Names:blr1632
OrganismiBradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Taxonomic identifieri224911 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
ProteomesiUP000002526: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 608607Glutamine--fructose-6-phosphate aminotransferase [isomerizing]UniRule annotation
PRO_0000135434Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi224911.blr1632.

Structurei

3D structure databases

ProteinModelPortaliP94323.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 217216Glutamine amidotransferase type-2
Add
BLAST
Domaini284 – 423140SIS 1
Add
BLAST
Domaini456 – 598143SIS 2
Add
BLAST

Sequence similaritiesi

Contains 2 SIS domains.

Keywords - Domaini

Glutamine amidotransferase, Repeat

Phylogenomic databases

eggNOGiCOG0449.
HOGENOMiHOG000258896.
KOiK00820.
OrthoDBiEOG6KT2Q1.
PhylomeDBiP94323.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
HAMAPiMF_00164. GlmS.
InterProiIPR017932. GATase_2_dom.
IPR005855. GlmS_trans.
IPR029055. Ntn_hydrolases_N.
IPR001347. SIS.
[Graphical view]
PANTHERiPTHR10937:SF0. PTHR10937:SF0. 1 hit.
PfamiPF01380. SIS. 2 hits.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01135. glmS. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS51464. SIS. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P94323-1 [UniParc]FASTAAdd to Basket

« Hide

MCGIVGILGR GPVVDKLVAS LRRLEYRGYD SAGLATLEGV RIERRRAEGK    50
LRNLEEQLRY CPPSGHAGIG HTRWATHGKP TESNAHPHAT ENVAVVHNGI 100
IENFRELRAE LERNGAGFNS ETDTEVVAHL VDSYLKNGYS PQDAVQASLP 150
RLRGAFALAF LFKANDDLLI GACKGSPLAI GHGRGEVYLG SDAIALAPLT 200
DTVTYLEDGD WAVLTRATCV IYGADGSIVQ RETSKSGVSA LLVDKANYRH 250
FMAKEIHEQP TVAGKTLAHY LDVAAKRVAL PLALPFDFNC IQRISITACG 300
TASYAGHIAK YWFERLARLP CDVDVASEFR YREAPLRRGD LAIVISQSGE 350
TADTLAALRY AKGKGLHTIS VVNVPTSTIA RESESVLPTL AGPEIGVAST 400
KAFICQLMVL GVLAVRAAKE RGKLSEIDES QLVRELIEVP RLIAAALLVE 450
PQIEKLARYI AGARTVLYLG RGTSAPLALE GALKLKEISY IHSEGYAAGE 500
LKHGPIALID EAVPVVVIAP YDEVFEKTVS NMQEVAARGG KIILITDAKG 550
ASEAMVDTLL TIVLPAMVAS FTPLVYAIPV QLLAYHTAVA RGADVDQPRN 600
LAKSVTVE 608
Length:608
Mass (Da):65,390
Last modified:January 23, 2007 - v3
Checksum:i32C12A4AB7FD1D76
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti331 – 3311Y → N in AAB39494. 1 Publication
Sequence conflicti335 – 3417PLRRGDL → LAPRQF in AAB39494. 1 Publication
Sequence conflicti346 – 3461S → L in AAB39494. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000040 Genomic DNA. Translation: BAC46897.1.
U67278 Genomic DNA. Translation: AAB39494.1.
RefSeqiNP_768272.1. NC_004463.1.

Genome annotation databases

EnsemblBacteriaiBAC46897; BAC46897; BAC46897.
GeneIDi1053361.
KEGGibja:blr1632.
PATRICi21186808. VBIBraJap65052_1665.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000040 Genomic DNA. Translation: BAC46897.1 .
U67278 Genomic DNA. Translation: AAB39494.1 .
RefSeqi NP_768272.1. NC_004463.1.

3D structure databases

ProteinModelPortali P94323.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224911.blr1632.

Protein family/group databases

MEROPSi C44.971.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAC46897 ; BAC46897 ; BAC46897 .
GeneIDi 1053361.
KEGGi bja:blr1632.
PATRICi 21186808. VBIBraJap65052_1665.

Phylogenomic databases

eggNOGi COG0449.
HOGENOMi HOG000258896.
KOi K00820.
OrthoDBi EOG6KT2Q1.
PhylomeDBi P94323.

Enzyme and pathway databases

BioCyci BJAP224911:GJEJ-1651-MONOMER.

Family and domain databases

Gene3Di 3.60.20.10. 1 hit.
HAMAPi MF_00164. GlmS.
InterProi IPR017932. GATase_2_dom.
IPR005855. GlmS_trans.
IPR029055. Ntn_hydrolases_N.
IPR001347. SIS.
[Graphical view ]
PANTHERi PTHR10937:SF0. PTHR10937:SF0. 1 hit.
Pfami PF01380. SIS. 2 hits.
[Graphical view ]
SUPFAMi SSF56235. SSF56235. 1 hit.
TIGRFAMsi TIGR01135. glmS. 1 hit.
PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
PS51464. SIS. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110.
  2. "The Bradyrhizobium japonicum noeD gene: a negatively acting, genotype-specific nodulation gene for soybean."
    Lohrke S.M., Day B., Kolli V.S., Hancock R., Yuen J.P., de Souza M.L., Stacey G., Carlson R., Tong Z., Hur H.G., Orf J.H., Sadowsky M.J.
    Mol. Plant Microbe Interact. 11:476-488(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 328-608.
    Strain: JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110.

Entry informationi

Entry nameiNODM_BRADU
AccessioniPrimary (citable) accession number: P94323
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 96 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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