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P94323

- NODM_BRADU

UniProt

P94323 - NODM_BRADU

Protein

Glutamine--fructose-6-phosphate aminotransferase [isomerizing]

Gene

nodM

Organism
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Involved in the production of the root hair deformation (HAD) factor specifically on soybean.By similarity

    Catalytic activityi

    L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei2 – 21Nucleophile; for GATase activityBy similarity
    Active sitei603 – 6031For Fru-6P isomerization activityBy similarity

    GO - Molecular functioni

    1. carbohydrate binding Source: InterPro
    2. glutamine-fructose-6-phosphate transaminase (isomerizing) activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. carbohydrate biosynthetic process Source: InterPro
    2. glutamine metabolic process Source: UniProtKB-HAMAP
    3. nodulation Source: UniProtKB-KW

    Keywords - Molecular functioni

    Aminotransferase, Transferase

    Keywords - Biological processi

    Nodulation

    Enzyme and pathway databases

    BioCyciBJAP224911:GJEJ-1651-MONOMER.

    Protein family/group databases

    MEROPSiC44.971.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamine--fructose-6-phosphate aminotransferase [isomerizing] (EC:2.6.1.16)
    Short name:
    GFAT
    Alternative name(s):
    Nodulation protein M
    Gene namesi
    Name:nodM
    Ordered Locus Names:blr1632
    OrganismiBradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
    Taxonomic identifieri224911 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
    ProteomesiUP000002526: Chromosome

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 608607Glutamine--fructose-6-phosphate aminotransferase [isomerizing]PRO_0000135434Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi224911.blr1632.

    Structurei

    3D structure databases

    ProteinModelPortaliP94323.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini2 – 217216Glutamine amidotransferase type-2Add
    BLAST
    Domaini284 – 423140SIS 1Add
    BLAST
    Domaini456 – 598143SIS 2Add
    BLAST

    Sequence similaritiesi

    Contains 2 SIS domains.Curated

    Keywords - Domaini

    Glutamine amidotransferase, Repeat

    Phylogenomic databases

    eggNOGiCOG0449.
    HOGENOMiHOG000258896.
    KOiK00820.
    OrthoDBiEOG6KT2Q1.
    PhylomeDBiP94323.

    Family and domain databases

    Gene3Di3.60.20.10. 1 hit.
    HAMAPiMF_00164. GlmS.
    InterProiIPR017932. GATase_2_dom.
    IPR005855. GlmS_trans.
    IPR029055. Ntn_hydrolases_N.
    IPR001347. SIS.
    [Graphical view]
    PANTHERiPTHR10937:SF0. PTHR10937:SF0. 1 hit.
    PfamiPF01380. SIS. 2 hits.
    [Graphical view]
    SUPFAMiSSF56235. SSF56235. 1 hit.
    TIGRFAMsiTIGR01135. glmS. 1 hit.
    PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
    PS51464. SIS. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P94323-1 [UniParc]FASTAAdd to Basket

    « Hide

    MCGIVGILGR GPVVDKLVAS LRRLEYRGYD SAGLATLEGV RIERRRAEGK    50
    LRNLEEQLRY CPPSGHAGIG HTRWATHGKP TESNAHPHAT ENVAVVHNGI 100
    IENFRELRAE LERNGAGFNS ETDTEVVAHL VDSYLKNGYS PQDAVQASLP 150
    RLRGAFALAF LFKANDDLLI GACKGSPLAI GHGRGEVYLG SDAIALAPLT 200
    DTVTYLEDGD WAVLTRATCV IYGADGSIVQ RETSKSGVSA LLVDKANYRH 250
    FMAKEIHEQP TVAGKTLAHY LDVAAKRVAL PLALPFDFNC IQRISITACG 300
    TASYAGHIAK YWFERLARLP CDVDVASEFR YREAPLRRGD LAIVISQSGE 350
    TADTLAALRY AKGKGLHTIS VVNVPTSTIA RESESVLPTL AGPEIGVAST 400
    KAFICQLMVL GVLAVRAAKE RGKLSEIDES QLVRELIEVP RLIAAALLVE 450
    PQIEKLARYI AGARTVLYLG RGTSAPLALE GALKLKEISY IHSEGYAAGE 500
    LKHGPIALID EAVPVVVIAP YDEVFEKTVS NMQEVAARGG KIILITDAKG 550
    ASEAMVDTLL TIVLPAMVAS FTPLVYAIPV QLLAYHTAVA RGADVDQPRN 600
    LAKSVTVE 608
    Length:608
    Mass (Da):65,390
    Last modified:January 23, 2007 - v3
    Checksum:i32C12A4AB7FD1D76
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti331 – 3311Y → N in AAB39494. (PubMed:9612946)Curated
    Sequence conflicti335 – 3417PLRRGDL → LAPRQF in AAB39494. (PubMed:9612946)Curated
    Sequence conflicti346 – 3461S → L in AAB39494. (PubMed:9612946)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000040 Genomic DNA. Translation: BAC46897.1.
    U67278 Genomic DNA. Translation: AAB39494.1.
    RefSeqiNP_768272.1. NC_004463.1.

    Genome annotation databases

    EnsemblBacteriaiBAC46897; BAC46897; BAC46897.
    GeneIDi1053361.
    KEGGibja:blr1632.
    PATRICi21186808. VBIBraJap65052_1665.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000040 Genomic DNA. Translation: BAC46897.1 .
    U67278 Genomic DNA. Translation: AAB39494.1 .
    RefSeqi NP_768272.1. NC_004463.1.

    3D structure databases

    ProteinModelPortali P94323.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224911.blr1632.

    Protein family/group databases

    MEROPSi C44.971.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAC46897 ; BAC46897 ; BAC46897 .
    GeneIDi 1053361.
    KEGGi bja:blr1632.
    PATRICi 21186808. VBIBraJap65052_1665.

    Phylogenomic databases

    eggNOGi COG0449.
    HOGENOMi HOG000258896.
    KOi K00820.
    OrthoDBi EOG6KT2Q1.
    PhylomeDBi P94323.

    Enzyme and pathway databases

    BioCyci BJAP224911:GJEJ-1651-MONOMER.

    Family and domain databases

    Gene3Di 3.60.20.10. 1 hit.
    HAMAPi MF_00164. GlmS.
    InterProi IPR017932. GATase_2_dom.
    IPR005855. GlmS_trans.
    IPR029055. Ntn_hydrolases_N.
    IPR001347. SIS.
    [Graphical view ]
    PANTHERi PTHR10937:SF0. PTHR10937:SF0. 1 hit.
    Pfami PF01380. SIS. 2 hits.
    [Graphical view ]
    SUPFAMi SSF56235. SSF56235. 1 hit.
    TIGRFAMsi TIGR01135. glmS. 1 hit.
    PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
    PS51464. SIS. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110.
    2. "The Bradyrhizobium japonicum noeD gene: a negatively acting, genotype-specific nodulation gene for soybean."
      Lohrke S.M., Day B., Kolli V.S., Hancock R., Yuen J.P., de Souza M.L., Stacey G., Carlson R., Tong Z., Hur H.G., Orf J.H., Sadowsky M.J.
      Mol. Plant Microbe Interact. 11:476-488(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 328-608.
      Strain: JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110.

    Entry informationi

    Entry nameiNODM_BRADU
    AccessioniPrimary (citable) accession number: P94323
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 97 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3