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Protein

Glutamine--fructose-6-phosphate aminotransferase [isomerizing]

Gene

nodM

Organism
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the production of the root hair deformation (HAD) factor specifically on soybean.By similarity

Catalytic activityi

L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21Nucleophile; for GATase activityBy similarity
Active sitei603 – 6031For Fru-6P isomerization activityBy similarity

GO - Molecular functioni

  1. carbohydrate binding Source: InterPro
  2. glutamine-fructose-6-phosphate transaminase (isomerizing) activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. carbohydrate biosynthetic process Source: InterPro
  2. glutamine metabolic process Source: UniProtKB-HAMAP
  3. nodulation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Keywords - Biological processi

Nodulation

Enzyme and pathway databases

BioCyciBJAP224911:GJEJ-1651-MONOMER.

Protein family/group databases

MEROPSiC44.971.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine--fructose-6-phosphate aminotransferase [isomerizing] (EC:2.6.1.16)
Short name:
GFAT
Alternative name(s):
Nodulation protein M
Gene namesi
Name:nodM
Ordered Locus Names:blr1632
OrganismiBradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Taxonomic identifieri224911 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
ProteomesiUP000002526: Chromosome

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 608607Glutamine--fructose-6-phosphate aminotransferase [isomerizing]PRO_0000135434Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi224911.blr1632.

Structurei

3D structure databases

ProteinModelPortaliP94323.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 217216Glutamine amidotransferase type-2Add
BLAST
Domaini284 – 423140SIS 1Add
BLAST
Domaini456 – 598143SIS 2Add
BLAST

Sequence similaritiesi

Contains 2 SIS domains.Curated

Keywords - Domaini

Glutamine amidotransferase, Repeat

Phylogenomic databases

eggNOGiCOG0449.
HOGENOMiHOG000258896.
InParanoidiP94323.
KOiK00820.
OrthoDBiEOG6KT2Q1.
PhylomeDBiP94323.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
HAMAPiMF_00164. GlmS.
InterProiIPR017932. GATase_2_dom.
IPR005855. GlmS_trans.
IPR029055. Ntn_hydrolases_N.
IPR001347. SIS.
[Graphical view]
PANTHERiPTHR10937:SF0. PTHR10937:SF0. 1 hit.
PfamiPF01380. SIS. 2 hits.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01135. glmS. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS51464. SIS. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P94323-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MCGIVGILGR GPVVDKLVAS LRRLEYRGYD SAGLATLEGV RIERRRAEGK
60 70 80 90 100
LRNLEEQLRY CPPSGHAGIG HTRWATHGKP TESNAHPHAT ENVAVVHNGI
110 120 130 140 150
IENFRELRAE LERNGAGFNS ETDTEVVAHL VDSYLKNGYS PQDAVQASLP
160 170 180 190 200
RLRGAFALAF LFKANDDLLI GACKGSPLAI GHGRGEVYLG SDAIALAPLT
210 220 230 240 250
DTVTYLEDGD WAVLTRATCV IYGADGSIVQ RETSKSGVSA LLVDKANYRH
260 270 280 290 300
FMAKEIHEQP TVAGKTLAHY LDVAAKRVAL PLALPFDFNC IQRISITACG
310 320 330 340 350
TASYAGHIAK YWFERLARLP CDVDVASEFR YREAPLRRGD LAIVISQSGE
360 370 380 390 400
TADTLAALRY AKGKGLHTIS VVNVPTSTIA RESESVLPTL AGPEIGVAST
410 420 430 440 450
KAFICQLMVL GVLAVRAAKE RGKLSEIDES QLVRELIEVP RLIAAALLVE
460 470 480 490 500
PQIEKLARYI AGARTVLYLG RGTSAPLALE GALKLKEISY IHSEGYAAGE
510 520 530 540 550
LKHGPIALID EAVPVVVIAP YDEVFEKTVS NMQEVAARGG KIILITDAKG
560 570 580 590 600
ASEAMVDTLL TIVLPAMVAS FTPLVYAIPV QLLAYHTAVA RGADVDQPRN

LAKSVTVE
Length:608
Mass (Da):65,390
Last modified:January 23, 2007 - v3
Checksum:i32C12A4AB7FD1D76
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti331 – 3311Y → N in AAB39494. (PubMed:9612946)Curated
Sequence conflicti335 – 3417PLRRGDL → LAPRQF in AAB39494. (PubMed:9612946)Curated
Sequence conflicti346 – 3461S → L in AAB39494. (PubMed:9612946)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000040 Genomic DNA. Translation: BAC46897.1.
U67278 Genomic DNA. Translation: AAB39494.1.
RefSeqiNP_768272.1. NC_004463.1.

Genome annotation databases

EnsemblBacteriaiBAC46897; BAC46897; BAC46897.
GeneIDi1053361.
KEGGibja:blr1632.
PATRICi21186808. VBIBraJap65052_1665.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000040 Genomic DNA. Translation: BAC46897.1.
U67278 Genomic DNA. Translation: AAB39494.1.
RefSeqiNP_768272.1. NC_004463.1.

3D structure databases

ProteinModelPortaliP94323.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224911.blr1632.

Protein family/group databases

MEROPSiC44.971.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAC46897; BAC46897; BAC46897.
GeneIDi1053361.
KEGGibja:blr1632.
PATRICi21186808. VBIBraJap65052_1665.

Phylogenomic databases

eggNOGiCOG0449.
HOGENOMiHOG000258896.
InParanoidiP94323.
KOiK00820.
OrthoDBiEOG6KT2Q1.
PhylomeDBiP94323.

Enzyme and pathway databases

BioCyciBJAP224911:GJEJ-1651-MONOMER.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
HAMAPiMF_00164. GlmS.
InterProiIPR017932. GATase_2_dom.
IPR005855. GlmS_trans.
IPR029055. Ntn_hydrolases_N.
IPR001347. SIS.
[Graphical view]
PANTHERiPTHR10937:SF0. PTHR10937:SF0. 1 hit.
PfamiPF01380. SIS. 2 hits.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01135. glmS. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS51464. SIS. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110.
  2. "The Bradyrhizobium japonicum noeD gene: a negatively acting, genotype-specific nodulation gene for soybean."
    Lohrke S.M., Day B., Kolli V.S., Hancock R., Yuen J.P., de Souza M.L., Stacey G., Carlson R., Tong Z., Hur H.G., Orf J.H., Sadowsky M.J.
    Mol. Plant Microbe Interact. 11:476-488(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 328-608.
    Strain: JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110.

Entry informationi

Entry nameiNODM_BRADU
AccessioniPrimary (citable) accession number: P94323
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 23, 2007
Last modified: January 7, 2015
This is version 99 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.