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Protein

Glutamine--fructose-6-phosphate aminotransferase [isomerizing]

Gene

nodM

Organism
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the production of the root hair deformation (HAD) factor specifically on soybean.By similarity

Catalytic activityi

L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei2Nucleophile; for GATase activityBy similarity1
Active sitei603For Fru-6P isomerization activityBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Keywords - Biological processi

Nodulation

Protein family/group databases

MEROPSiC44.971.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine--fructose-6-phosphate aminotransferase [isomerizing] (EC:2.6.1.16)
Short name:
GFAT
Alternative name(s):
Nodulation protein M
Gene namesi
Name:nodM
Ordered Locus Names:blr1632
OrganismiBradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Taxonomic identifieri224911 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
Proteomesi
  • UP000002526 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001354342 – 608Glutamine--fructose-6-phosphate aminotransferase [isomerizing]Add BLAST607

Interactioni

Protein-protein interaction databases

STRINGi224911.blr1632.

Structurei

3D structure databases

ProteinModelPortaliP94323.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 217Glutamine amidotransferase type-2Add BLAST216
Domaini284 – 423SIS 1Add BLAST140
Domaini456 – 598SIS 2Add BLAST143

Sequence similaritiesi

Contains 2 SIS domains.Curated

Keywords - Domaini

Glutamine amidotransferase, Repeat

Phylogenomic databases

eggNOGiENOG4105C46. Bacteria.
COG0449. LUCA.
HOGENOMiHOG000258896.
InParanoidiP94323.
KOiK00820.
OMAiRDSTICV.
OrthoDBiPOG091H0061.
PhylomeDBiP94323.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
HAMAPiMF_00164. GlmS. 1 hit.
InterProiIPR017932. GATase_2_dom.
IPR005855. GlmS_trans.
IPR029055. Ntn_hydrolases_N.
IPR001347. SIS.
[Graphical view]
PANTHERiPTHR10937:SF0. PTHR10937:SF0. 1 hit.
PfamiPF01380. SIS. 2 hits.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01135. glmS. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS51464. SIS. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P94323-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCGIVGILGR GPVVDKLVAS LRRLEYRGYD SAGLATLEGV RIERRRAEGK
60 70 80 90 100
LRNLEEQLRY CPPSGHAGIG HTRWATHGKP TESNAHPHAT ENVAVVHNGI
110 120 130 140 150
IENFRELRAE LERNGAGFNS ETDTEVVAHL VDSYLKNGYS PQDAVQASLP
160 170 180 190 200
RLRGAFALAF LFKANDDLLI GACKGSPLAI GHGRGEVYLG SDAIALAPLT
210 220 230 240 250
DTVTYLEDGD WAVLTRATCV IYGADGSIVQ RETSKSGVSA LLVDKANYRH
260 270 280 290 300
FMAKEIHEQP TVAGKTLAHY LDVAAKRVAL PLALPFDFNC IQRISITACG
310 320 330 340 350
TASYAGHIAK YWFERLARLP CDVDVASEFR YREAPLRRGD LAIVISQSGE
360 370 380 390 400
TADTLAALRY AKGKGLHTIS VVNVPTSTIA RESESVLPTL AGPEIGVAST
410 420 430 440 450
KAFICQLMVL GVLAVRAAKE RGKLSEIDES QLVRELIEVP RLIAAALLVE
460 470 480 490 500
PQIEKLARYI AGARTVLYLG RGTSAPLALE GALKLKEISY IHSEGYAAGE
510 520 530 540 550
LKHGPIALID EAVPVVVIAP YDEVFEKTVS NMQEVAARGG KIILITDAKG
560 570 580 590 600
ASEAMVDTLL TIVLPAMVAS FTPLVYAIPV QLLAYHTAVA RGADVDQPRN

LAKSVTVE
Length:608
Mass (Da):65,390
Last modified:January 23, 2007 - v3
Checksum:i32C12A4AB7FD1D76
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti331Y → N in AAB39494 (PubMed:9612946).Curated1
Sequence conflicti335 – 341PLRRGDL → LAPRQF in AAB39494 (PubMed:9612946).Curated7
Sequence conflicti346S → L in AAB39494 (PubMed:9612946).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000040 Genomic DNA. Translation: BAC46897.1.
U67278 Genomic DNA. Translation: AAB39494.1.
RefSeqiNP_768272.1. NC_004463.1.
WP_011084447.1. NZ_CP011360.1.

Genome annotation databases

EnsemblBacteriaiBAC46897; BAC46897; BAC46897.
GeneIDi1053361.
KEGGibja:blr1632.
PATRICi21186808. VBIBraJap65052_1665.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000040 Genomic DNA. Translation: BAC46897.1.
U67278 Genomic DNA. Translation: AAB39494.1.
RefSeqiNP_768272.1. NC_004463.1.
WP_011084447.1. NZ_CP011360.1.

3D structure databases

ProteinModelPortaliP94323.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224911.blr1632.

Protein family/group databases

MEROPSiC44.971.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAC46897; BAC46897; BAC46897.
GeneIDi1053361.
KEGGibja:blr1632.
PATRICi21186808. VBIBraJap65052_1665.

Phylogenomic databases

eggNOGiENOG4105C46. Bacteria.
COG0449. LUCA.
HOGENOMiHOG000258896.
InParanoidiP94323.
KOiK00820.
OMAiRDSTICV.
OrthoDBiPOG091H0061.
PhylomeDBiP94323.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
HAMAPiMF_00164. GlmS. 1 hit.
InterProiIPR017932. GATase_2_dom.
IPR005855. GlmS_trans.
IPR029055. Ntn_hydrolases_N.
IPR001347. SIS.
[Graphical view]
PANTHERiPTHR10937:SF0. PTHR10937:SF0. 1 hit.
PfamiPF01380. SIS. 2 hits.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01135. glmS. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS51464. SIS. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNODM_BRADU
AccessioniPrimary (citable) accession number: P94323
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 106 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.