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Protein

RNA polymerase sigma factor RpoD

Gene

sigA

Organism
Bradyrhizobium japonicum
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is the primary sigma factor during exponential growth.SAAS annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi679 – 698H-T-H motifUniRule annotationAdd BLAST20

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-bindingUniRule annotationSAAS annotation, Sigma factorUniRule annotationSAAS annotation
Biological processTranscription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
RNA polymerase sigma factor RpoDUniRule annotation
Alternative name(s):
Sigma-70UniRule annotation
Gene namesi
Name:sigAImported
Synonyms:rpoDUniRule annotation
ORF Names:AF336_39140Imported
OrganismiBradyrhizobium japonicumImported
Taxonomic identifieri375 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
Proteomesi
  • UP000037708 Componenti: Unassembled WGS sequence

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Interacts transiently with the RNA polymerase catalytic core.UniRule annotationSAAS annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini509 – 522RNA_pol_sigma70InterPro annotationAdd BLAST14
Domaini678 – 704RNA_pol_sigma70InterPro annotationAdd BLAST27

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni485 – 555Sigma-70 factor domain-2UniRule annotationAdd BLAST71
Regioni564 – 640Sigma-70 factor domain-3UniRule annotationAdd BLAST77
Regioni653 – 706Sigma-70 factor domain-4UniRule annotationAdd BLAST54

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili471 – 491Sequence analysisAdd BLAST21

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi509 – 512Interaction with polymerase core subunit RpoCUniRule annotation4

Sequence similaritiesi

Belongs to the sigma-70 factor family. RpoD/SigA subfamily.SAAS annotation

Keywords - Domaini

Coiled coilSequence analysis

Phylogenomic databases

eggNOGiENOG4105DG1 Bacteria
COG0568 LUCA
HOGENOMiHOG000270272

Family and domain databases

Gene3Di1.10.10.10, 2 hits
HAMAPiMF_00963 Sigma70_RpoD_SigA, 1 hit
InterProiView protein in InterPro
IPR014284 RNA_pol_sigma-70_dom
IPR000943 RNA_pol_sigma70
IPR009042 RNA_pol_sigma70_r1_2
IPR007627 RNA_pol_sigma70_r2
IPR007624 RNA_pol_sigma70_r3
IPR007630 RNA_pol_sigma70_r4
IPR007631 RNA_pol_sigma_70_non-ess
IPR007127 RNA_pol_sigma_70_r1_1
IPR013325 RNA_pol_sigma_r2
IPR013324 RNA_pol_sigma_r3/r4-like
IPR012760 RNA_pol_sigma_RpoD_C
IPR028630 Sigma70_RpoD
IPR036388 WH-like_DNA-bd_sf
PfamiView protein in Pfam
PF04546 Sigma70_ner, 1 hit
PF03979 Sigma70_r1_1, 1 hit
PF00140 Sigma70_r1_2, 1 hit
PF04542 Sigma70_r2, 1 hit
PF04539 Sigma70_r3, 1 hit
PF04545 Sigma70_r4, 1 hit
PRINTSiPR00046 SIGMA70FCT
SUPFAMiSSF88659 SSF88659, 2 hits
SSF88946 SSF88946, 2 hits
TIGRFAMsiTIGR02393 RpoD_Cterm, 1 hit
TIGR02937 sigma70-ECF, 1 hit
PROSITEiView protein in PROSITE
PS00715 SIGMA70_1, 1 hit
PS00716 SIGMA70_2, 1 hit

Sequencei

Sequence statusi: Complete.

P94321-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATKAKTLQA KDKEKDDKAA DAPEKDSQDA PSPLLDLSDA AVKKMIKQAK
60 70 80 90 100
KRGFVTFDQL NEVLPSDQTS PEQIEDIMSM LSDMGINVTE ADDSEGEEDK
110 120 130 140 150
DEGGEDETDN ELVEVTQKAV TEVKKSEPGE RTDDPVRMYL REMGTVELLS
160 170 180 190 200
REGEIAIAKR IEAGREAMIA GLCESPLSFQ AIIIWRDELN EGKIFLRDII
210 220 230 240 250
DLEATYAGPE AKGGMNTAMI GGPTGENGEA TAEGGEAVAV TGAAPAHVAP
260 270 280 290 300
PAAPPAPTPF RAAPAAGNGA EAEKDPGEAA AEADMDEDDE FENQMSLAAI
310 320 330 340 350
EAELKPKVVE IFDKIAESYK KLRKLQEQDI QNQLESTSHG PSLSPHQERK
360 370 380 390 400
YRKLKDEIIV EVKSLRLNQA RIDSLVEQLY DINKRLVSHE GRLMRLADSH
410 420 430 440 450
GVAREDFLRN YTGSELDPRW LNRVSKLSAK GWKNFVHHEK DRIKDLRHEV
460 470 480 490 500
HQLAALTGLE IVEFRKIVHS VQKGEREARQ AKKEMVEANL RLVISIAKKY
510 520 530 540 550
TNRGLQFLDL IQEGNIGLMK AVDKFEYRRG YKFSTYATWW IRQAITRSIA
560 570 580 590 600
DQARTIRIPV HMIETINKIV RTSRQMLNEI GREPTPEELA EKLGMPLEKV
610 620 630 640 650
RKVLKIAKEP LSLETPVGDE EDSHLGDFIE DKNAILPIDA AIQSNLRETT
660 670 680 690 700
TRVLASLTPR EERVLRMRFG IGMNTDHTLE EVGQQFSVTR ERIRQIEAKA
710
LRKLKHPSRS RKLRSFLDN
Length:719
Mass (Da):80,652
Last modified:May 1, 1997 - v1
Checksum:i4B0A6B87F8D43162
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X99588 Genomic DNA Translation: CAA67902.1
LGUJ01000044 Genomic DNA Translation: KOY04978.1
RefSeqiWP_011090084.1, NZ_LGUJ01000044.1

Genome annotation databases

EnsemblBacteriaiKOY04978; KOY04978; AF336_39140
PATRICifig|375.38.peg.7052

Similar proteinsi

Entry informationi

Entry nameiP94321_BRAJP
AccessioniPrimary (citable) accession number: P94321
Secondary accession number(s): D4AFB0, E7FHX8, Q79U39
Entry historyiIntegrated into UniProtKB/TrEMBL: May 1, 1997
Last sequence update: May 1, 1997
Last modified: April 25, 2018
This is version 128 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

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