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P94316 (DHE2_BACFR) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
NAD-specific glutamate dehydrogenase
Short name=NAD-GDH
EC=1.4.1.2
Alternative name(s):
NADH-dependent glutamate dehydrogenase
Gene names
Name:gdhB
Ordered Locus Names:BF3635
OrganismBacteroides fragilis (strain YCH46) [Complete proteome] [HAMAP]
Taxonomic identifier295405 [NCBI]
Taxonomic lineageBacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length445 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

L-glutamate + H2O + NAD+ = 2-oxoglutarate + NH3 + NADH.

Subunit structure

Homohexamer By similarity.

Induction

By high peptide concentrations.

Sequence similarities

Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

Ontologies

Keywords
   LigandNAD
   Molecular functionOxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processcellular amino acid metabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular functionglutamate dehydrogenase (NAD+) activity

Inferred from electronic annotation. Source: EC

nucleotide binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 445445NAD-specific glutamate dehydrogenase
PRO_0000182765

Regions

Nucleotide binding235 – 2417NAD Potential

Sites

Active site1241 By similarity

Experimental info

Sequence conflict441S → A in AAC26399. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P94316 [UniParc].

Last modified November 23, 2004. Version 2.
Checksum: E7462E4E6963217B

FASTA44548,390
        10         20         30         40         50         60 
MNIEKIMSSL EAKHPGESEY LQAVKEVLLS IEDIYNQHPE FEKSKIIERL VEPDRIFTFR 

        70         80         90        100        110        120 
VTWVDDKGEV QTNLGYRVQF NNAIGPYKGG IRFHASVNLS ILKFLGFEQT FKNALTTLPM 

       130        140        150        160        170        180 
GGGKGGSDFS PRGKSDAEIM RFCQAFMLEL WRHLGPDMDV PAGDIGVGGR EVGYMFGMYK 

       190        200        210        220        230        240 
KLTREFTGTF TGKGLEFGGS LIRPEATGFG GLYFVNQMLQ TKGIDIKGKT VAISGFGNVA 

       250        260        270        280        290        300 
WGAATKATEL GAKVVTISGP DGYIYDPNGI SGEKIDYMLE LRASGNDIVA PYADEFPGST 

       310        320        330        340        350        360 
FVAGKRPWEV KADIALPCAT QNELNGEDAK NLIDNNVLCV GEISNMGCTP EAIDLFIEHK 

       370        380        390        400        410        420 
TMYAPGKAVN AGGVATSGLE MSQNAMHLSW SAAEVDEKLH SIMHGIHAQC VKYGTEPDGY 

       430        440 
INYVKGANIA GFMKVAHAMM GQGII

« Hide

References

« Hide 'large scale' references
[1]"The NADH-dependent glutamate dehydrogenase enzyme of Bacteroides fragilis Bf1 is induced by peptides in the growth medium."
Abrahams G.L., Abratt V.R.
Microbiology 144:1659-1667(1998) [PubMed: 9639936] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: BF1.
[2]"Genomic analysis of Bacteroides fragilis reveals extensive DNA inversions regulating cell surface adaptation."
Kuwahara T., Yamashita A., Hirakawa H., Nakayama H., Toh H., Okada N., Kuhara S., Hattori M., Hayashi T., Ohnishi Y.
Proc. Natl. Acad. Sci. U.S.A. 101:14919-14924(2004) [PubMed: 15466707] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: YCH46.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U78108 Genomic DNA. Translation: AAC26399.1.
AP006841 Genomic DNA. Translation: BAD50378.1.
RefSeqYP_100912.1. NC_006347.1.

3D structure databases

ProteinModelPortalP94316.
SMRP94316. Positions 2-444.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3084717.
GenomeReviewsGene locus BF3635 in contig AP006841_GR.
KEGGbfr:BF3635.
NMPDRfig|295405.3.peg.3689.
PATRIC21052818. VBIBacFra17906_3488.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG590661.
OMAGNVAWGA.
ProtClustDBPRK14030.

Enzyme and pathway databases

BioCycBFRA295405:BF3635-MONOMER.

Family and domain databases

InterProIPR006095. Glu/Leu/Phe/Val_DH.
IPR006096. Glu/Leu/Phe/Val_DH_C.
IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
IPR014362. Glu_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
Gene3DG3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KOK00262.
PfamPF00208. ELFV_dehydrog. 1 hit.
PF02812. ELFV_dehydrog_N. 1 hit.
[Graphical view]
PIRSFPIRSF000185. Glu_DH. 1 hit.
PRINTSPR00082. GLFDHDRGNASE.
SMARTSM00839. ELFV_dehydrog. 1 hit.
[Graphical view]
PROSITEPS00074. GLFV_DEHYDROGENASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDHE2_BACFR
AccessionPrimary (citable) accession number: P94316
Secondary accession number(s): Q64Q53
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 23, 2004
Last modified: January 25, 2012
This is version 73 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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