Reviewed, UniProtKB/Swiss-Prot P94316 (DHE2_BACFR)

Names and origin

Protein names

Recommended name:
    NAD-specific glutamate dehydrogenase
      Short name=NAD-GDH
    EC=1.4.1.2
Alternative name(s):
    NADH-dependent glutamate dehydrogenase

Gene names

Name:	gdhB
Ordered Locus Names:	BF3635

Organism

Bacteroides fragilis [Complete proteome] [HAMAP]

Taxonomic identifier

817 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	445 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	L-glutamate + H2O + NAD+ = 2-oxoglutarate + NH3 + NADH.
Subunit structure	Homohexamer By similarity.
Induction	By high peptide concentrations.
Sequence similarities	Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular amino acid metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro glutamate dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 445	445	NAD-specific glutamate dehydrogenase		PRO_0000182765
Regions
	Nucleotide binding	235 – 241	7	NAD Potential
Sites
	Active site	124	1	By similarity
Experimental info
	Sequence conflict	44	1	S → A in AAC26399. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P94316-1 [UniParc]. Last modified November 23, 2004. Version 2. Checksum: E7462E4E6963217B	FASTA	445	48,390
	10 20 30 40 50 60 MNIEKIMSSL EAKHPGESEY LQAVKEVLLS IEDIYNQHPE FEKSKIIERL VEPDRIFTFR 70 80 90 100 110 120 VTWVDDKGEV QTNLGYRVQF NNAIGPYKGG IRFHASVNLS ILKFLGFEQT FKNALTTLPM 130 140 150 160 170 180 GGGKGGSDFS PRGKSDAEIM RFCQAFMLEL WRHLGPDMDV PAGDIGVGGR EVGYMFGMYK 190 200 210 220 230 240 KLTREFTGTF TGKGLEFGGS LIRPEATGFG GLYFVNQMLQ TKGIDIKGKT VAISGFGNVA 250 260 270 280 290 300 WGAATKATEL GAKVVTISGP DGYIYDPNGI SGEKIDYMLE LRASGNDIVA PYADEFPGST 310 320 330 340 350 360 FVAGKRPWEV KADIALPCAT QNELNGEDAK NLIDNNVLCV GEISNMGCTP EAIDLFIEHK 370 380 390 400 410 420 TMYAPGKAVN AGGVATSGLE MSQNAMHLSW SAAEVDEKLH SIMHGIHAQC VKYGTEPDGY 430 440 INYVKGANIA GFMKVAHAMM GQGII

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The NADH-dependent glutamate dehydrogenase enzyme of Bacteroides fragilis Bf1 is induced by peptides in the growth medium." Abrahams G.L., Abratt V.R. Microbiology 144:1659-1667(1998) [PubMed: 9639936] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: BF1.
[2]	"Genomic analysis of Bacteroides fragilis reveals extensive DNA inversions regulating cell surface adaptation." Kuwahara T., Yamashita A., Hirakawa H., Nakayama H., Toh H., Okada N., Kuhara S., Hattori M., Hayashi T., Ohnishi Y. Proc. Natl. Acad. Sci. U.S.A. 101:14919-14924(2004) [PubMed: 15466707] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: YCH46.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U78108 Genomic DNA. Translation: AAC26399.1. AP006841 Genomic DNA. Translation: BAD50378.1.
RefSeq	YP_100912.1.
3D structure databases
SMR	P94316. Positions 2-444.
ModBase	Search...
Genome annotation databases
GeneID	3084717.
GenomeReviews	Gene locus BF3635 in contig AP006841_GR.
KEGG	bfr:BF3635.
NMPDR	fig|295405.3.peg.3689.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG590661.
OMA	WEVKADI.
Enzyme and pathway databases
BioCyc	BFRA295405:BF3635-MONOMER.
BRENDA	1.4.1.2. 868.
Family and domain databases
InterPro	IPR006095. Glu/Leu/Phe/Val_DH. IPR006096. Glu/Leu/Phe/Val_DH_C. IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom. IPR014362. Glu_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR11606:SF2. GLFV_DH. 1 hit.
Pfam	PF00208. ELFV_dehydrog. 1 hit. PF02812. ELFV_dehydrog_N. 1 hit. [Graphical view]
PIRSF	PIRSF000185. Glu_DH. 1 hit.
PRINTS	PR00082. GLFDHDRGNASE.
SMART	SM00839. ELFV_dehydrog. 1 hit. [Graphical view]
PROSITE	PS00074. GLFV_DEHYDROGENASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHE2_BACFR

Accession

Primary (citable) accession number: P94316
Secondary accession number(s): Q64Q53

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 23, 2004
Last modified:	January 19, 2010
This is version 60 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)