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P94286

- CTA1_BACCI

UniProt

P94286 - CTA1_BACCI

Protein

Cycloisomaltooligosaccharide glucanotransferase

Gene
N/A
Organism
Bacillus circulans
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 59 (01 Oct 2014)
      Sequence version 1 (01 May 1997)
      Previous versions | rss
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    Functioni

    Produces cycloisomaltooligosaccharide from dextran containing 7, 8 or 9 glucose units. The enzyme is specific for (1->6)-alpha-D-glucans (dextrans) and, without activity toward (1->4)-alpha-D-glucans, such as amylose. It also has no activity on oligosaccharides, such as amylopectin and pullulan, containing (1->6)-alpha-D-glucosidic linkages at branch points.1 Publication

    Catalytic activityi

    Cyclizes part of a (1->6)-alpha-D-glucan chain by formation of a (1->6)-alpha-D-glucosidic bond.1 Publication

    Kineticsi

    1. KM=0.063 mM for dextran T-401 Publication

    Vmax=0.872 pmol/min/µg enzyme with dextran T-40 as substrate1 Publication

    pH dependencei

    Optimum pH is 5.5.2 Publications

    GO - Molecular functioni

    1. carbohydrate binding Source: InterPro
    2. transferase activity, transferring glycosyl groups Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Protein family/group databases

    CAZyiCBM35. Carbohydrate-Binding Module Family 35.
    GH66. Glycoside Hydrolase Family 66.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cycloisomaltooligosaccharide glucanotransferase (EC:2.4.1.248)
    Short name:
    CITase
    OrganismiBacillus circulans
    Taxonomic identifieri1397 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi183 – 1831D → N: Has 1% of wild-type activity. 1 Publication
    Mutagenesisi308 – 3081D → N: No activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3838Sequence AnalysisAdd
    BLAST
    Chaini39 – 972934Cycloisomaltooligosaccharide glucanotransferasePRO_0000012241Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.1 Publication

    Structurei

    Secondary structure

    1
    972
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi44 – 507
    Beta strandi52 – 543
    Beta strandi59 – 679
    Beta strandi70 – 723
    Beta strandi74 – 8411
    Beta strandi87 – 9812
    Beta strandi103 – 1119
    Beta strandi114 – 12512
    Helixi127 – 1293
    Beta strandi131 – 1388
    Helixi142 – 1443
    Beta strandi148 – 1514
    Helixi160 – 17415
    Beta strandi178 – 1814
    Beta strandi185 – 1873
    Beta strandi201 – 2044
    Beta strandi210 – 2123
    Helixi213 – 22513
    Beta strandi229 – 24214
    Helixi244 – 2463
    Helixi250 – 2523
    Beta strandi253 – 2575
    Beta strandi265 – 2673
    Beta strandi269 – 2724
    Beta strandi274 – 2785
    Helixi283 – 30018
    Beta strandi303 – 3086
    Beta strandi315 – 3184
    Helixi326 – 3283
    Helixi330 – 34415
    Helixi346 – 3483
    Beta strandi350 – 3578
    Helixi365 – 3717
    Beta strandi375 – 3806
    Beta strandi383 – 3853
    Helixi388 – 40114
    Beta strandi407 – 4104
    Turni413 – 4164
    Beta strandi420 – 4245
    Helixi425 – 4273
    Beta strandi428 – 4325
    Beta strandi434 – 4363
    Beta strandi439 – 4413
    Beta strandi447 – 4515
    Beta strandi457 – 47721
    Beta strandi480 – 4823
    Beta strandi484 – 4907
    Beta strandi493 – 5008
    Beta strandi504 – 5074
    Beta strandi509 – 51911
    Beta strandi521 – 5299
    Beta strandi538 – 54710
    Helixi550 – 56213
    Beta strandi566 – 5683
    Turni572 – 5743
    Beta strandi579 – 5813
    Beta strandi586 – 5883
    Helixi591 – 60616
    Helixi608 – 6125
    Beta strandi621 – 6233
    Beta strandi626 – 6283
    Beta strandi633 – 6364
    Beta strandi641 – 6488
    Beta strandi650 – 65910
    Beta strandi665 – 6695
    Beta strandi677 – 68610
    Beta strandi692 – 6987
    Helixi703 – 7053
    Beta strandi708 – 7103
    Beta strandi713 – 7175
    Beta strandi720 – 73819

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3WNKX-ray2.30A39-738[»]
    3WNLX-ray2.60A39-738[»]
    3WNMX-ray2.25A39-738[»]
    3WNNX-ray2.25A/B39-738[»]
    3WNOX-ray1.90A/B39-738[»]
    3WNPX-ray2.80A/B39-738[»]
    ProteinModelPortaliP94286.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini421 – 546126CBM6 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini748 – 871124CBM6 2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 66 family.Curated
    Contains 2 CBM6 (carbohydrate binding type-6) domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Signal

    Family and domain databases

    Gene3Di2.60.120.260. 2 hits.
    InterProiIPR005084. CMB_fam6.
    IPR008979. Galactose-bd-like.
    IPR025092. Glyco_hydro_66.
    [Graphical view]
    PfamiPF03422. CBM_6. 2 hits.
    PF13199. Glyco_hydro_66. 1 hit.
    [Graphical view]
    SUPFAMiSSF49785. SSF49785. 2 hits.
    PROSITEiPS51175. CBM6. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P94286-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVRFMYALRK RRLSLLLAMS LLVMCVASVV SPPPQALASG SGGIERVFTD    50
    KARYNPGDAV SIRVQAKNGT GSSWSGAARL EIFHLENSVY TSSQSLSLTN 100
    GQSTTLTFTW TAPSTDFRGY FVRIDAGTLG QGATAIDVSS DFTKYPRYGY 150
    ISEFESGETA LESKAKVDQL AQDYHINAWQ FYDWMWRHDK MIKRTGGSID 200
    STWLDLFNRE ISWSTLQNQI DAVHDVNGKA MAYAMIYASR ENYSPLGISP 250
    TWGIYEDSSH TNQFDVDFGD GSTYLYMSDP QNPNWQNYIH AEYIDSINTA 300
    GFDGIHVDQM GQRSNVYDYN GNSIDLSTRF SPFLDQAKSV LSANNPARDN 350
    LTYNIVDGTV NGWAVNDVSK NADLDFLYSE IWYLSDSYNQ LKNYIEQLRA 400
    NGGNKAVVLA AYMNYADNAG TRYEAESASM TNVSTNTNHA GYTGSGFVDQ 450
    FASTGDKVSF AINAPEAGDY SLVFRYGNNT GANSTLNLYV DGNFVQKLYF 500
    FNQSSWGTWK HDAWYQVPLT QGAHTVELRY ESGNVGAVNL DSLTLGTFDE 550
    HSVRLADAMM SASGATHIEL GDDNQMLPHE YYPNRSKTMR SSLKNAMKDH 600
    YNFITAYENL LFDSDVVPND TGSQFVNLTG VSASGDGSAN TVWYINKRTS 650
    DYNIVHLINL LGNDNQWRNT ASQPSFQTNL PAKIYIGADE TISDVYLASP 700
    DLSGGETQEL AFTSGTDAGG KYVSFTVPEL KYWNMIYMKR TFSVPANDIY 750
    EAETAIKSNV STNTNHAGYT GSGFVDGFSS TNDGVSFVVK STASDDYALR 800
    FRYANGGSDA TRDVYVDGKL AGTVSFKSTG SWSTWSYGEI TARLEPGHHT 850
    IVLWQTSGNT GAINLDHLDL DKTYIWQFDR QIVSVPAGYR ITFRTGLPGW 900
    VHWGVNGWTG VTDTPLRSNG SLDGNLDHET SIGPFATGTA VDVTFLWDDN 950
    NNGILEPSTD RWEGTDFGIN VS 972
    Length:972
    Mass (Da):107,431
    Last modified:May 1, 1997 - v1
    Checksum:i2827BEA61782CC22
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D61382 Genomic DNA. Translation: BAA09604.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D61382 Genomic DNA. Translation: BAA09604.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3WNK X-ray 2.30 A 39-738 [» ]
    3WNL X-ray 2.60 A 39-738 [» ]
    3WNM X-ray 2.25 A 39-738 [» ]
    3WNN X-ray 2.25 A/B 39-738 [» ]
    3WNO X-ray 1.90 A/B 39-738 [» ]
    3WNP X-ray 2.80 A/B 39-738 [» ]
    ProteinModelPortali P94286.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi CBM35. Carbohydrate-Binding Module Family 35.
    GH66. Glycoside Hydrolase Family 66.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 2.60.120.260. 2 hits.
    InterProi IPR005084. CMB_fam6.
    IPR008979. Galactose-bd-like.
    IPR025092. Glyco_hydro_66.
    [Graphical view ]
    Pfami PF03422. CBM_6. 2 hits.
    PF13199. Glyco_hydro_66. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49785. SSF49785. 2 hits.
    PROSITEi PS51175. CBM6. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and sequence analysis of the cycloisomaltooligosaccharide glucanotransferase gene from Bacillus circulans T-3040 and expression in Escherichia coli cells."
      Oguma T., Kurokawa T., Tobe K., Kobayashi M.
      J. Appl. Glycosci. 42:415-419(1995)
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: T-3040.
    2. "Purification and properties of a novel enzyme from Bacillus spp. T-3040, which catalyzes the conversion of dextran to cyclic isomaltooligosaccharides."
      Oguma T., Tobe K., Kobayashi M.
      FEBS Lett. 345:135-138(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBUNIT, PH DEPENDENCE.
      Strain: T-3040.
    3. Erratum
      Oguma T., Tobe K., Kobayashi M.
      FEBS Lett. 349:442-442(1994)
    4. "Identification of catalytic amino acids of cyclodextran glucanotransferase from Bacillus circulans T-3040."
      Yamamoto T., Terasawa K., Kim Y.M., Kimura A., Kitamura Y., Kobayashi M., Funane K.
      Biosci. Biotechnol. Biochem. 70:1947-1953(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF ASP-183 AND ASP-308, BIOPHYSICOCHEMICAL PROPERTIES.
      Strain: T-3040.

    Entry informationi

    Entry nameiCTA1_BACCI
    AccessioniPrimary (citable) accession number: P94286
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: May 1, 1997
    Last modified: October 1, 2014
    This is version 59 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3