P94286 (CTA1_BACCI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Cycloisomaltooligosaccharide glucanotransferase Short name=CITase EC=2.4.1.248
Organism	Bacillus circulans
Taxonomic identifier	1397 [NCBI]
Taxonomic lineage	Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	972 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Produces cycloisomaltooligosaccharide from dextran containing 7, 8 or 9 glucose units. The enzyme is specific for (1->6)-alpha-D-glucans (dextrans) and, without activity toward (1->4)-alpha-D-glucans, such as amylose. It also has no activity on oligosaccharides, such as amylopectin and pullulan, containing (1->6)-alpha-D-glucosidic linkages at branch points. Ref.2
Catalytic activity	Cyclizes part of a (1->6)-alpha-D-glucan chain by formation of a (1->6)-alpha-D-glucosidic bond. Ref.2
Subunit structure	Monomer. Ref.2
Sequence similarities	Belongs to the glycosyl hydrolase 66 family. Contains 2 CBM6 (carbohydrate binding type-6) domains.
Biophysicochemical properties	Kinetic parameters: KM=0.063 mM for dextran T-40 Ref.4 Vmax=0.872 pmol/min/µg enzyme with dextran T-40 as substrate pH dependence: Optimum pH is 5.5.

Ontologies

Keywords
Domain	Repeat Signal
Molecular function	Glycosyltransferase Transferase
Gene Ontology (GO)
Molecular function	carbohydrate binding Inferred from electronic annotation. Source: InterPro transferase activity, transferring glycosyl groups Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 38	38	Potential
	Chain	39 – 972	934	Cycloisomaltooligosaccharide glucanotransferase		PRO_0000012241
Regions
	Domain	421 – 546	126	CBM6 1
	Domain	748 – 871	124	CBM6 2
Experimental info
	Mutagenesis	183	1	D → N: Has 1% of wild-type activity. Ref.4
	Mutagenesis	308	1	D → N: No activity. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P94286 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 2827BEA61782CC22	FASTA	972	107,431
	10 20 30 40 50 60 MVRFMYALRK RRLSLLLAMS LLVMCVASVV SPPPQALASG SGGIERVFTD KARYNPGDAV 70 80 90 100 110 120 SIRVQAKNGT GSSWSGAARL EIFHLENSVY TSSQSLSLTN GQSTTLTFTW TAPSTDFRGY 130 140 150 160 170 180 FVRIDAGTLG QGATAIDVSS DFTKYPRYGY ISEFESGETA LESKAKVDQL AQDYHINAWQ 190 200 210 220 230 240 FYDWMWRHDK MIKRTGGSID STWLDLFNRE ISWSTLQNQI DAVHDVNGKA MAYAMIYASR 250 260 270 280 290 300 ENYSPLGISP TWGIYEDSSH TNQFDVDFGD GSTYLYMSDP QNPNWQNYIH AEYIDSINTA 310 320 330 340 350 360 GFDGIHVDQM GQRSNVYDYN GNSIDLSTRF SPFLDQAKSV LSANNPARDN LTYNIVDGTV 370 380 390 400 410 420 NGWAVNDVSK NADLDFLYSE IWYLSDSYNQ LKNYIEQLRA NGGNKAVVLA AYMNYADNAG 430 440 450 460 470 480 TRYEAESASM TNVSTNTNHA GYTGSGFVDQ FASTGDKVSF AINAPEAGDY SLVFRYGNNT 490 500 510 520 530 540 GANSTLNLYV DGNFVQKLYF FNQSSWGTWK HDAWYQVPLT QGAHTVELRY ESGNVGAVNL 550 560 570 580 590 600 DSLTLGTFDE HSVRLADAMM SASGATHIEL GDDNQMLPHE YYPNRSKTMR SSLKNAMKDH 610 620 630 640 650 660 YNFITAYENL LFDSDVVPND TGSQFVNLTG VSASGDGSAN TVWYINKRTS DYNIVHLINL 670 680 690 700 710 720 LGNDNQWRNT ASQPSFQTNL PAKIYIGADE TISDVYLASP DLSGGETQEL AFTSGTDAGG 730 740 750 760 770 780 KYVSFTVPEL KYWNMIYMKR TFSVPANDIY EAETAIKSNV STNTNHAGYT GSGFVDGFSS 790 800 810 820 830 840 TNDGVSFVVK STASDDYALR FRYANGGSDA TRDVYVDGKL AGTVSFKSTG SWSTWSYGEI 850 860 870 880 890 900 TARLEPGHHT IVLWQTSGNT GAINLDHLDL DKTYIWQFDR QIVSVPAGYR ITFRTGLPGW 910 920 930 940 950 960 VHWGVNGWTG VTDTPLRSNG SLDGNLDHET SIGPFATGTA VDVTFLWDDN NNGILEPSTD 970 RWEGTDFGIN VS

References

[1]	"Cloning and sequence analysis of the cycloisomaltooligosaccharide glucanotransferase gene from Bacillus circulans T-3040 and expression in Escherichia coli cells." Oguma T., Kurokawa T., Tobe K., Kobayashi M. J. Appl. Glycosci. 42:415-419(1995) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: T-3040.
[2]	"Purification and properties of a novel enzyme from Bacillus spp. T-3040, which catalyzes the conversion of dextran to cyclic isomaltooligosaccharides." Oguma T., Tobe K., Kobayashi M. FEBS Lett. 345:135-138(1994) [PubMed: 7515357] [Abstract] Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBUNIT, PH DEPENDENCE. Strain: T-3040.
[3]	Erratum Oguma T., Tobe K., Kobayashi M. FEBS Lett. 349:442-442(1994)
[4]	"Identification of catalytic amino acids of cyclodextran glucanotransferase from Bacillus circulans T-3040." Yamamoto T., Terasawa K., Kim Y.M., Kimura A., Kitamura Y., Kobayashi M., Funane K. Biosci. Biotechnol. Biochem. 70:1947-1953(2006) [PubMed: 16926507] [Abstract] Cited for: MUTAGENESIS OF ASP-183 AND ASP-308, BIOPHYSICOCHEMICAL PROPERTIES. Strain: T-3040.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D61382 Genomic DNA. Translation: BAA09604.1.
3D structure databases
ProteinModelPortal	P94286.
ModBase	Search...
Protein family/group databases
CAZy	CBM35. Carbohydrate-Binding Module Family 35. GH66. Glycoside Hydrolase Family 66.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR005084. CMB_fam6. IPR008979. Galactose-bd-like. [Graphical view]
Pfam	PF03422. CBM_6. 2 hits. [Graphical view]
SUPFAM	SSF49785. Gal_bind_like. 2 hits.
PROSITE	PS51175. CBM6. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CTA1_BACCI

Accession

Primary (citable) accession number: P94286

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	May 1, 1997
Last modified:	November 16, 2011
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program