P94283 (SYFB_BORBU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phenylalanine--tRNA ligase beta chain
EC=6.1.1.20

Alternative name(s):
Phenylalanyl-tRNA synthetase beta chain
Short name=PheRS

Gene names

Name:	pheT
Ordered Locus Names:	BB_0514

Organism

Borrelia burgdorferi (Lyme disease spirochete)

Taxonomic identifier

139 [NCBI]

Taxonomic lineage

Bacteria › Spirochaetes › Spirochaetales › Spirochaetaceae › Borrelia › Borrelia burgdorferi group

Protein attributes

Sequence length	566 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP MF_00284
Subunit structure	Tetramer of two alpha and two beta chains By similarity.
Subcellular location	Cytoplasm HAMAP MF_00284.
Sequence similarities	Belongs to the phenylalanyl-tRNA synthetase beta chain family. Type 2 subfamily. Contains 1 B5 domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	phenylalanyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: InterPro phenylalanine-tRNA ligase activity Inferred from electronic annotation. Source: EC tRNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 566	566	Phenylalanine--tRNA ligase beta chain HAMAP MF_00284		PRO_0000126996
Regions
	Domain	287 – 362	76	B5

Sequences

	Sequence		Length	Mass (Da)
	P94283 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 9D48C8B5D6D3B74B	FASTA	566	65,174
	10 20 30 40 50 60 MPKLEIYKNL FLDKIGKNFT NLEISELLEP FKAEFDGFDE SSGKIKIEFN DTNRPDLWSY 70 80 90 100 110 120 LGLARQIKTY FFGEMPYYDF FSKKGDFKKF YGEILVDGKM SQIRPFIFGF LAKGLIINDK 130 140 150 160 170 180 MLETLIQFQE KLCQNYGQKR RRIAMGMYNS NFIKFPISYI ASSPNHKFVP FGMDYELSLL 190 200 210 220 230 240 EINEKHPKGL EYSHIIKNFD KFPLLLDDNN NVVSYPPIIN SNNIGSLKVG DTDLFVEVTG 250 260 270 280 290 300 IDFEATLLAL SIAACDFYDM GFEILPVKTV FREPFNLDFK ELVCPYYFQE EVEFNVENIN 310 320 330 340 350 360 RLLGSNLTLE RICLSLKKMG VNSYSKDFKN YIVPPFYRND FLHEVDVIED VMIGEGLSSF 370 380 390 400 410 420 NPELPKAFAV GRLSPLEEFS RNVRNLMVGM GFQEMIYNYM GSKKDFIDRM NINDQNFLKV 430 440 450 460 470 480 SNPMTENYEY IRASIIPNLL KSESVSSNFP YPHKIFEIGK VALKNLDTTE GTSTFTNLAF 490 500 510 520 530 540 LMSGKEISFN EINSIVATLF YYLNIEINLI ESKTTFYING RGADIVIEGF NIGGFGEISP 550 560 YVLNNFGIFI PCSVFEVNIN KLMSRS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Phenylalanyl-tRNA synthetase genes (alpha and beta subunits) and thioredoxin reductase gene of Borrelia burgdorferi." Barbour A.G., Hinnebusch J. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 35210 / B31 / CIP 102532 / DSM 4680.
[2]	"Genomic sequence of a Lyme disease spirochaete, Borrelia burgdorferi." Fraser C.M., Casjens S., Huang W.M., Sutton G.G., Clayton R.A., Lathigra R., White O., Ketchum K.A., Dodson R.J., Hickey E.K., Gwinn M.L., Dougherty B.A., Tomb J.-F., Fleischmann R.D., Richardson D.L., Peterson J.D., Kerlavage A.R., Quackenbush J. , Salzberg S.L., Hanson M., van Vugt R., Palmer N., Adams M.D., Gocayne J.D., Weidman J.F., Utterback T.R., Watthey L., McDonald L.A., Artiach P., Bowman C., Garland S.A., Fujii C., Cotton M.D., Horst K., Roberts K.M., Hatch B., Smith H.O., Venter J.C. Nature 390:580-586(1997) [PubMed: 9403685] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35210 / B31 / CIP 102532 / DSM 4680.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U82978 Genomic DNA. Translation: AAB41019.1. AE000783 Genomic DNA. Translation: AAC66870.1.
PIR	A70164.
RefSeq	NP_212648.1. NC_001318.1.
3D structure databases
ProteinModelPortal	P94283.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBORT00000008496; EBBORP00000007826; EBBORG00000008495.
GeneID	1195360.
GenomeReviews	Gene locus BB_0514 in contig AE000783_GR.
KEGG	bbu:BB0514.
NMPDR	fig|224326.1.peg.898.
PATRIC	20557652. VBIBorBur75917_0905.
TIGR	BB_0514.
Phylogenomic databases
GeneTree	EBGT00050000007386.
HOGENOM	HBG497667.
OMA	SECCKIT.
PhylomeDB	P94283.
ProtClustDB	PRK09616.
Enzyme and pathway databases
BioCyc	BBUR224326:BB_0514-MONOMER.
Family and domain databases
HAMAP	MF_00284. Phe_tRNA_synth_beta2. [Tree]
InterPro	IPR005146. B3/B4_tRNA-bd. IPR009061. DNA-bd_dom_put. IPR004531. Phe-tRNA-synth_IIc_bsu_arc. IPR022918. Phe_tRNA_synth_beta2_bac/arc. IPR002319. Phenylalanyl-tRNA_Synthase. IPR005147. tRNA_synthase_B5-dom. [Graphical view]
Gene3D	G3DSA:3.30.56.20. B5. 1 hit.
KO	K01890.
Pfam	PF03484. B5. 1 hit. PF01409. tRNA-synt_2d. 1 hit. [Graphical view]
SMART	SM00873. B3_4. 1 hit. SM00874. B5. 1 hit. [Graphical view]
SUPFAM	SSF46955. Putativ_DNA_bind. 2 hits.
TIGRFAMs	TIGR00471. PheT_arch. 1 hit.
PROSITE	PS51483. B5. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYFB_BORBU

Accession

Primary (citable) accession number: P94283

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1997
Last modified:	January 25, 2012
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program