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P94282 (SYFA_BORBU) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phenylalanine--tRNA ligase alpha chain

EC=6.1.1.20
Alternative name(s):
Phenylalanyl-tRNA synthetase alpha chain
Short name=PheRS
Gene names
Name:pheS
Ordered Locus Names:BB_0513
OrganismBorrelia burgdorferi (Lyme disease spirochete)
Taxonomic identifier139 [NCBI]
Taxonomic lineageBacteriaSpirochaetesSpirochaetalesSpirochaetaceaeBorreliaBorrelia burgdorferi group

Protein attributes

Sequence length528 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP MF_00282

Cofactor

Binds 2 magnesium ions per tetramer By similarity. HAMAP MF_00282

Subunit structure

Tetramer of two alpha and two beta chains By similarity.

Subcellular location

Cytoplasm HAMAP MF_00282.

Sequence similarities

Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 2 subfamily.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 528528Phenylalanine--tRNA ligase alpha chain HAMAP MF_00282
PRO_0000126804

Experimental info

Sequence conflict1461T → S in AAB41018. Ref.2
Sequence conflict407 – 41812IVIED…SIKTL → LLLRMVLALKLC Ref.2

Sequences

Sequence LengthMass (Da)Tools
P94282 [UniParc].

Last modified May 30, 2000. Version 2.
Checksum: C5C97D529BBD6D07

FASTA52859,952
        10         20         30         40         50         60 
MKVIFMKADL NLIKTLHPLE IKVIVNNEEE DDISASIIIE KLGFNEGQAN KTIEWLNSKG 

        70         80         90        100        110        120 
IIEEIYRKLN VFYKATERGL GALRDGFVEE KIINLVSQKA VLASNLALEL DIDVKEVRKA 

       130        140        150        160        170        180 
FGNLLKEGIL SLDLDKQIII NCLDGTETNY QKVRVLLERA KNSDLLRESL TTEELLLISN 

       190        200        210        220        230        240 
FAKKKGADSV FFKIIEKLDL KFRLSSFGLE VKNFLMKSKL TGDELTKLTP EILKNKTYEN 

       250        260        270        280        290        300 
KKFRAYNIHI PSAKTFIGRA NSYLDYISKI KDKLVGLGFQ EFDGPLVETE FFNNDALFMP 

       310        320        330        340        350        360 
QFHPSRDIKD VYYISDPSMQ ESLPEPYFSN VKLAHETGYA TGSRGWRYSF SEDLSKRLVL 

       370        380        390        400        410        420 
RTHGTVLSAK QLINAKNPSR YFGVIRCFRY DQVDATHGVD FYQTEGIVIE DGVSIKTLLG 

       430        440        450        460        470        480 
LLEIFAKELA GATEIKYVPA YFPFTEPSIE IHVKHPVLGW FELGGSGIFR PEVTKPLGID 

       490        500        510        520 
LPVIAWGIGI DRMALMHLGL NDLRDLFTYD IGDVILRRGK IDAKVRNL 

« Hide

References

« Hide 'large scale' references
[1]"Genomic sequence of a Lyme disease spirochaete, Borrelia burgdorferi."
Fraser C.M., Casjens S., Huang W.M., Sutton G.G., Clayton R.A., Lathigra R., White O., Ketchum K.A., Dodson R.J., Hickey E.K., Gwinn M.L., Dougherty B.A., Tomb J.-F., Fleischmann R.D., Richardson D.L., Peterson J.D., Kerlavage A.R., Quackenbush J. expand/collapse author list , Salzberg S.L., Hanson M., van Vugt R., Palmer N., Adams M.D., Gocayne J.D., Weidman J.F., Utterback T.R., Watthey L., McDonald L.A., Artiach P., Bowman C., Garland S.A., Fujii C., Cotton M.D., Horst K., Roberts K.M., Hatch B., Smith H.O., Venter J.C.
Nature 390:580-586(1997) [PubMed: 9403685] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 35210 / B31 / CIP 102532 / DSM 4680.
[2]"Phenylalanyl-tRNA synthetase genes (alpha and beta subunits) and thioredoxin reductase gene of Borrelia burgdorferi."
Barbour A.G., Hinnebusch J.
Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 6-418.
Strain: ATCC 35210 / B31 / CIP 102532 / DSM 4680.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE000783 Genomic DNA. Translation: AAC66871.1.
U82978 Genomic DNA. Translation: AAB41018.1.
PIRH70163.
RefSeqNP_212647.1. NC_001318.1.

3D structure databases

ProteinModelPortalP94282.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBBORT00000008705; EBBORP00000008035; EBBORG00000008704.
GeneID1195359.
GenomeReviewsGene locus BB_0513 in contig AE000783_GR.
KEGGbbu:BB0513.
NMPDRfig|224326.1.peg.897.
PATRIC20557650. VBIBorBur75917_0904.
TIGRBB_0513.

Phylogenomic databases

GeneTreeEBGT00050000007094.
HOGENOMHBG497668.
OMAWFELGGS.
PhylomeDBP94282.
ProtClustDBPRK04172.

Enzyme and pathway databases

BioCycBBUR224326:BB_0513-MONOMER.

Family and domain databases

HAMAPMF_00282. Phe_tRNA_synth_alpha2.
[Tree]
InterProIPR006195. aa-tRNA-synth_II.
IPR004529. Phe-tRNA-synth_IIc_asu.
IPR022917. Phe_tRNA_synth_alpha2_bac/arc.
IPR002319. Phenylalanyl-tRNA_Synthase.
[Graphical view]
KOK01889.
PfamPF01409. tRNA-synt_2d. 1 hit.
[Graphical view]
TIGRFAMsTIGR00468. PheS. 1 hit.
PROSITEPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYFA_BORBU
AccessionPrimary (citable) accession number: P94282
Secondary accession number(s): O51466
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 30, 2000
Last modified: January 25, 2012
This is version 87 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

SIMILARITY comments

Index of protein domains and families