Reviewed, UniProtKB/Swiss-Prot P94210 (DHLO_AGRVI)

Names and origin

Protein names

Recommended name:
    Protein ocs
Including the following 2 domains:
    1- Recommended name:
            D-lysopine dehydrogenase
              EC=1.5.1.16
        Alternative name(s):
            Lysopine synthase
    2- Recommended name:
            D-octopine dehydrogenase
              EC=1.5.1.11
        Alternative name(s):
            Octopine synthase

Gene names

Name:

ocs

Encoded on

Plasmid pTiTM4

Organism

Agrobacterium vitis (Rhizobium vitis)

Taxonomic identifier

373 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium

Protein attributes

Sequence length	358 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Reductive condensation of pyruvate and arginine, lysine, histidine, or octopine to form octopine, lysopine, histopine, or octopinic acid, respectively. NADPH is the preferred cofactor, but NADH can also be used.
Catalytic activity	N(2)-(D-1-carboxyethyl)-L-arginine + NAD+ + H2O = L-arginine + pyruvate + NADH. N(2)-(D-1-carboxyethyl)-L-lysine + NADP+ + H2O = L-lysine + pyruvate + NADPH.
Sequence similarities	Belongs to the lysopine/nopaline/octopine/opine/vitopine dehydrogenases family.

Ontologies

Keywords
Disease	Crown gall tumor
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	Plasmid
Gene Ontology (GO)
Biological process	glycerol-3-phosphate catabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	D-lysopine dehydrogenase activity Inferred from electronic annotation. Source: EC D-octopine dehydrogenase activity Inferred from electronic annotation. Source: EC NAD or NADH binding Inferred from electronic annotation. Source: InterPro oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 358	358	Protein ocs		PRO_0000179980

Sequences

	Sequence		Length	Mass (Da)
	P94210-1 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 83F7D389BD16498F	FASTA	358	38,860
	10 20 30 40 50 60 MAKVAILGAG NLALTLAGDL ARRLGQTPSI WAPISNRSSF NDVRCLGSLE LVGPDYGGDF 70 80 90 100 110 120 QPRLEDDLGT AISGAAFIFL TVPTLGQQGI LRELAKFNLS NSVLVALPGS ATSLACKQTL 130 140 150 160 170 180 VPTFAPIAVI ESTTSPYACR RVKARVLMLG VKATFEVATT QPLSEEVKGR FEVLFPNPPQ 190 200 210 220 230 240 WYQHPASIFF SNTNPVAHPA GILAARDSIE QGILPVPKFY RQFVPQAITR VIAIDEERLS 250 260 270 280 290 300 IVDALGLESE TDFSYSKKWY GGHACNAREF YETYEGYAEI ETPKTMNHRY LTEDVKHILV 310 320 330 340 350 LWVEIAEAIG VQVPEMKSVV QEASDVLNEN LLRTGRGLSS LNLGGANANA IVRALNGV

References

[1]	Otten L., de Ruffray P. Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: CG474.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U83987 Genomic DNA. Translation: AAB41877.1.
3D structure databases
SMR	P94210. Positions 2-335.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.5.1.11. 97084. 1.5.1.16. 97084.
Family and domain databases
InterPro	IPR008927. 6-PGluconate_DH_C-like. IPR013328. DH_multihelical. IPR016040. NAD(P)-bd_dom. IPR011128. NAD-dep_Gly3P_DH_N. IPR003421. Opine_DH. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:1.10.1040.10. Opine_DH. 1 hit.
Pfam	PF01210. NAD_Gly3P_dh_N. 1 hit. PF02317. Octopine_DH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHLO_AGRVI

Accession

Primary (citable) accession number: P94210

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	May 1, 1997
Last modified:	January 19, 2010
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)