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P94147

- MTA1_THAGE

UniProt

P94147 - MTA1_THAGE

Protein

Modification methylase AgeI

Gene

ageIM

Organism
Thalassobius gelatinovorus (Ruegeria gelatinovora)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 63 (01 Oct 2014)
      Sequence version 1 (01 May 1997)
      Previous versions | rss
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    Functioni

    This methylase recognizes the double-stranded sequence ACCGGT, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the AgeI endonuclease.

    Catalytic activityi

    S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei80 – 801PROSITE-ProRule annotation

    GO - Molecular functioni

    1. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
    2. DNA binding Source: InterPro

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Protein family/group databases

    REBASEi3276. M.AgeI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Modification methylase AgeI (EC:2.1.1.37)
    Short name:
    M.AgeI
    Alternative name(s):
    Cytosine-specific methyltransferase AgeI
    Gene namesi
    Name:ageIM
    OrganismiThalassobius gelatinovorus (Ruegeria gelatinovora)
    Taxonomic identifieri53501 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeThalassobius

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 429429Modification methylase AgeIPRO_0000087856Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP94147.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 429429SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
    Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    InterProiIPR018117. C5_DNA_meth_AS.
    IPR001525. C5_MeTfrase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PANTHERiPTHR10629. PTHR10629. 1 hit.
    PfamiPF00145. DNA_methylase. 1 hit.
    [Graphical view]
    PRINTSiPR00105. C5METTRFRASE.
    SUPFAMiSSF53335. SSF53335. 2 hits.
    TIGRFAMsiTIGR00675. dcm. 1 hit.
    PROSITEiPS00094. C5_MTASE_1. 1 hit.
    PS00095. C5_MTASE_2. 1 hit.
    PS51679. SAM_MT_C5. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P94147-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKTIDLFCGA GGLGEGFRQA GFSALYANDH ETPALATYKE NHPDAVCSTD    50
    SIETVDPKKI REDLGVAPGQ VDVVMGGPPC QGFSTYGQRR DDDARNQLYV 100
    PYFGFVEEFR PKAFLIENVV GLLSMSGGAV LADMVARAEA LGYAADVVTL 150
    DACEYGVPQH RRRVFIFGAA DGQRIDPPQP SHVNGKRSGV VLNDQPSLFF 200
    DGPSIQPALT VRDAISDLPD EVLVPRDTQK PMEYPEPPKT EYQRLMRGNS 250
    TELTHHSAKR MLGIRRLRLA MLHPGDYGTK IEERLADGGL NDELIDLMMG 300
    GAGMRDAAEC RTQDREKEAA LREVLKGGHT TPAKVMEFLD SQGFANKYRR 350
    LRWDAPSHTV VAHMARDCSD FVHPGIDRFV SVREAARFQS FPDTYRFPGS 400
    QFRQFRQIGN AVPPLLGRAM AETIKVAIS 429
    Length:429
    Mass (Da):47,359
    Last modified:May 1, 1997 - v1
    Checksum:i97C5EC5414B02FD3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D78259 Genomic DNA. Translation: BAA11333.1.
    AF247972 Genomic DNA. Translation: AAF71525.1.
    PIRiJC4656.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D78259 Genomic DNA. Translation: BAA11333.1 .
    AF247972 Genomic DNA. Translation: AAF71525.1 .
    PIRi JC4656.

    3D structure databases

    ProteinModelPortali P94147.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 3276. M.AgeI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    InterProi IPR018117. C5_DNA_meth_AS.
    IPR001525. C5_MeTfrase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    PANTHERi PTHR10629. PTHR10629. 1 hit.
    Pfami PF00145. DNA_methylase. 1 hit.
    [Graphical view ]
    PRINTSi PR00105. C5METTRFRASE.
    SUPFAMi SSF53335. SSF53335. 2 hits.
    TIGRFAMsi TIGR00675. dcm. 1 hit.
    PROSITEi PS00094. C5_MTASE_1. 1 hit.
    PS00095. C5_MTASE_2. 1 hit.
    PS51679. SAM_MT_C5. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and nucleotide sequence of the AgeI methylase gene from Agrobacterium gelatinovorum IAM 12617, a marine bacterium."
      Suzuki T., Sugimoto E., Tahara Y., Yamada Y.
      Biosci. Biotechnol. Biochem. 60:444-447(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 25655 / DSM 5887 / IAM 12617 / JCM 20688 / NBRC 15761 / NCIMB / B6.
    2. "Method for cloning and producing AgeI restriction endonuclease in E.coli."
      Xu S.-Y., Maunus R.E., Lunnen K.D., Allen R.
      Patent number EP0959131, 26-APR-1999
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 25655 / DSM 5887 / IAM 12617 / JCM 20688 / NBRC 15761 / NCIMB / B6.

    Entry informationi

    Entry nameiMTA1_THAGE
    AccessioniPrimary (citable) accession number: P94147
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 8, 2000
    Last sequence update: May 1, 1997
    Last modified: October 1, 2014
    This is version 63 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3