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Protein

Modification methylase AgeI

Gene

ageIM

Organism
Thalassobius gelatinovorus (Ruegeria gelatinovora)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence ACCGGT, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the AgeI endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei80PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processRestriction system
LigandS-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase AgeI (EC:2.1.1.37)
Short name:
M.AgeI
Alternative name(s):
Cytosine-specific methyltransferase AgeI
Gene namesi
Name:ageIM
OrganismiThalassobius gelatinovorus (Ruegeria gelatinovora)
Taxonomic identifieri53501 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeThalassobius

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000878561 – 429Modification methylase AgeIAdd BLAST429

Structurei

3D structure databases

ProteinModelPortaliP94147
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 429SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd BLAST429

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation

Family and domain databases

InterProiView protein in InterPro
IPR018117 C5_DNA_meth_AS
IPR001525 C5_MeTfrase
IPR031303 C5_meth_CS
IPR029063 SAM-dependent_MTases
PfamiView protein in Pfam
PF00145 DNA_methylase, 1 hit
PRINTSiPR00105 C5METTRFRASE
SUPFAMiSSF53335 SSF53335, 2 hits
TIGRFAMsiTIGR00675 dcm, 1 hit
PROSITEiView protein in PROSITE
PS00094 C5_MTASE_1, 1 hit
PS00095 C5_MTASE_2, 1 hit
PS51679 SAM_MT_C5, 1 hit

Sequencei

Sequence statusi: Complete.

P94147-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTIDLFCGA GGLGEGFRQA GFSALYANDH ETPALATYKE NHPDAVCSTD
60 70 80 90 100
SIETVDPKKI REDLGVAPGQ VDVVMGGPPC QGFSTYGQRR DDDARNQLYV
110 120 130 140 150
PYFGFVEEFR PKAFLIENVV GLLSMSGGAV LADMVARAEA LGYAADVVTL
160 170 180 190 200
DACEYGVPQH RRRVFIFGAA DGQRIDPPQP SHVNGKRSGV VLNDQPSLFF
210 220 230 240 250
DGPSIQPALT VRDAISDLPD EVLVPRDTQK PMEYPEPPKT EYQRLMRGNS
260 270 280 290 300
TELTHHSAKR MLGIRRLRLA MLHPGDYGTK IEERLADGGL NDELIDLMMG
310 320 330 340 350
GAGMRDAAEC RTQDREKEAA LREVLKGGHT TPAKVMEFLD SQGFANKYRR
360 370 380 390 400
LRWDAPSHTV VAHMARDCSD FVHPGIDRFV SVREAARFQS FPDTYRFPGS
410 420
QFRQFRQIGN AVPPLLGRAM AETIKVAIS
Length:429
Mass (Da):47,359
Last modified:May 1, 1997 - v1
Checksum:i97C5EC5414B02FD3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D78259 Genomic DNA Translation: BAA11333.1
AF247972 Genomic DNA Translation: AAF71525.1
PIRiJC4656
RefSeqiWP_058263201.1, NZ_FOFW01000005.1

Similar proteinsi

Entry informationi

Entry nameiMTA1_THAGE
AccessioniPrimary (citable) accession number: P94147
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 8, 2000
Last sequence update: May 1, 1997
Last modified: March 28, 2018
This is version 73 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health