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P94147

- MTA1_THAGE

UniProt

P94147 - MTA1_THAGE

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Protein
Modification methylase AgeI
Gene
ageIM
Organism
Thalassobius gelatinovorus (Ruegeria gelatinovora)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence ACCGGT, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the AgeI endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei80 – 801 By similarity

GO - Molecular functioni

  1. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
  2. DNA binding Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3276. M.AgeI.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase AgeI (EC:2.1.1.37)
Short name:
M.AgeI
Alternative name(s):
Cytosine-specific methyltransferase AgeI
Gene namesi
Name:ageIM
OrganismiThalassobius gelatinovorus (Ruegeria gelatinovora)
Taxonomic identifieri53501 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeThalassobius

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 429429Modification methylase AgeI
PRO_0000087856Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP94147.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 429429SAM-dependent MTase C5-type
Add
BLAST

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERiPTHR10629. PTHR10629. 1 hit.
PfamiPF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 2 hits.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P94147-1 [UniParc]FASTAAdd to Basket

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MKTIDLFCGA GGLGEGFRQA GFSALYANDH ETPALATYKE NHPDAVCSTD    50
SIETVDPKKI REDLGVAPGQ VDVVMGGPPC QGFSTYGQRR DDDARNQLYV 100
PYFGFVEEFR PKAFLIENVV GLLSMSGGAV LADMVARAEA LGYAADVVTL 150
DACEYGVPQH RRRVFIFGAA DGQRIDPPQP SHVNGKRSGV VLNDQPSLFF 200
DGPSIQPALT VRDAISDLPD EVLVPRDTQK PMEYPEPPKT EYQRLMRGNS 250
TELTHHSAKR MLGIRRLRLA MLHPGDYGTK IEERLADGGL NDELIDLMMG 300
GAGMRDAAEC RTQDREKEAA LREVLKGGHT TPAKVMEFLD SQGFANKYRR 350
LRWDAPSHTV VAHMARDCSD FVHPGIDRFV SVREAARFQS FPDTYRFPGS 400
QFRQFRQIGN AVPPLLGRAM AETIKVAIS 429
Length:429
Mass (Da):47,359
Last modified:May 1, 1997 - v1
Checksum:i97C5EC5414B02FD3
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D78259 Genomic DNA. Translation: BAA11333.1.
AF247972 Genomic DNA. Translation: AAF71525.1.
PIRiJC4656.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D78259 Genomic DNA. Translation: BAA11333.1 .
AF247972 Genomic DNA. Translation: AAF71525.1 .
PIRi JC4656.

3D structure databases

ProteinModelPortali P94147.
ModBasei Search...

Protein family/group databases

REBASEi 3276. M.AgeI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
InterProi IPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
PANTHERi PTHR10629. PTHR10629. 1 hit.
Pfami PF00145. DNA_methylase. 1 hit.
[Graphical view ]
PRINTSi PR00105. C5METTRFRASE.
SUPFAMi SSF53335. SSF53335. 2 hits.
TIGRFAMsi TIGR00675. dcm. 1 hit.
PROSITEi PS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and nucleotide sequence of the AgeI methylase gene from Agrobacterium gelatinovorum IAM 12617, a marine bacterium."
    Suzuki T., Sugimoto E., Tahara Y., Yamada Y.
    Biosci. Biotechnol. Biochem. 60:444-447(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 25655 / DSM 5887 / IAM 12617 / JCM 20688 / NBRC 15761 / NCIMB / B6.
  2. "Method for cloning and producing AgeI restriction endonuclease in E.coli."
    Xu S.-Y., Maunus R.E., Lunnen K.D., Allen R.
    Patent number EP0959131, 26-APR-1999
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 25655 / DSM 5887 / IAM 12617 / JCM 20688 / NBRC 15761 / NCIMB / B6.

Entry informationi

Entry nameiMTA1_THAGE
AccessioniPrimary (citable) accession number: P94147
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 8, 2000
Last sequence update: May 1, 1997
Last modified: September 3, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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