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P94147 (MTA1_THAGE) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Modification methylase AgeI

Short name=M.AgeI
EC=2.1.1.37
Alternative name(s):
Cytosine-specific methyltransferase AgeI
Gene names
Name:ageIM
OrganismThalassobius gelatinovorus (Ruegeria gelatinovora)
Taxonomic identifier53501 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeThalassobius

Protein attributes

Sequence length429 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This methylase recognizes the double-stranded sequence ACCGGT, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the AgeI endonuclease.

Catalytic activity

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.

Contains 1 SAM-dependent MTase C5-type domain.

Ontologies

Keywords
   Biological processRestriction system
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA (cytosine-5-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

DNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 429429Modification methylase AgeI
PRO_0000087856

Regions

Domain1 – 429429SAM-dependent MTase C5-type

Sites

Active site801 By similarity

Sequences

Sequence LengthMass (Da)Tools
P94147 [UniParc].

Last modified May 1, 1997. Version 1.
Checksum: 97C5EC5414B02FD3

FASTA42947,359
        10         20         30         40         50         60 
MKTIDLFCGA GGLGEGFRQA GFSALYANDH ETPALATYKE NHPDAVCSTD SIETVDPKKI 

        70         80         90        100        110        120 
REDLGVAPGQ VDVVMGGPPC QGFSTYGQRR DDDARNQLYV PYFGFVEEFR PKAFLIENVV 

       130        140        150        160        170        180 
GLLSMSGGAV LADMVARAEA LGYAADVVTL DACEYGVPQH RRRVFIFGAA DGQRIDPPQP 

       190        200        210        220        230        240 
SHVNGKRSGV VLNDQPSLFF DGPSIQPALT VRDAISDLPD EVLVPRDTQK PMEYPEPPKT 

       250        260        270        280        290        300 
EYQRLMRGNS TELTHHSAKR MLGIRRLRLA MLHPGDYGTK IEERLADGGL NDELIDLMMG 

       310        320        330        340        350        360 
GAGMRDAAEC RTQDREKEAA LREVLKGGHT TPAKVMEFLD SQGFANKYRR LRWDAPSHTV 

       370        380        390        400        410        420 
VAHMARDCSD FVHPGIDRFV SVREAARFQS FPDTYRFPGS QFRQFRQIGN AVPPLLGRAM 


AETIKVAIS 

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References

[1]"Cloning and nucleotide sequence of the AgeI methylase gene from Agrobacterium gelatinovorum IAM 12617, a marine bacterium."
Suzuki T., Sugimoto E., Tahara Y., Yamada Y.
Biosci. Biotechnol. Biochem. 60:444-447(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 25655 / DSM 5887 / IAM 12617 / JCM 20688 / NBRC 15761 / NCIMB / B6.
[2]"Method for cloning and producing AgeI restriction endonuclease in E.coli."
Xu S.-Y., Maunus R.E., Lunnen K.D., Allen R.
Patent number EP0959131, 26-APR-1999
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 25655 / DSM 5887 / IAM 12617 / JCM 20688 / NBRC 15761 / NCIMB / B6.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D78259 Genomic DNA. Translation: BAA11333.1.
AF247972 Genomic DNA. Translation: AAF71525.1.
PIRJC4656.

3D structure databases

ProteinModelPortalP94147.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE3276. M.AgeI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.50.150. 1 hit.
InterProIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERPTHR10629. PTHR10629. 1 hit.
PfamPF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSPR00105. C5METTRFRASE.
SUPFAMSSF53335. SSF53335. 2 hits.
TIGRFAMsTIGR00675. dcm. 1 hit.
PROSITEPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMTA1_THAGE
AccessionPrimary (citable) accession number: P94147
Entry history
Integrated into UniProtKB/Swiss-Prot: December 8, 2000
Last sequence update: May 1, 1997
Last modified: June 11, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries