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P94147

- MTA1_THAGE

UniProt

P94147 - MTA1_THAGE

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Protein

Modification methylase AgeI

Gene

ageIM

Organism
Thalassobius gelatinovorus (Ruegeria gelatinovora)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence ACCGGT, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the AgeI endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei80 – 801PROSITE-ProRule annotation

GO - Molecular functioni

  1. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
  2. DNA binding Source: InterPro

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3276. M.AgeI.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase AgeI (EC:2.1.1.37)
Short name:
M.AgeI
Alternative name(s):
Cytosine-specific methyltransferase AgeI
Gene namesi
Name:ageIM
OrganismiThalassobius gelatinovorus (Ruegeria gelatinovora)
Taxonomic identifieri53501 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeThalassobius

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 429429Modification methylase AgeIPRO_0000087856Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP94147.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 429429SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERiPTHR10629. PTHR10629. 1 hit.
PfamiPF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 2 hits.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P94147-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKTIDLFCGA GGLGEGFRQA GFSALYANDH ETPALATYKE NHPDAVCSTD
60 70 80 90 100
SIETVDPKKI REDLGVAPGQ VDVVMGGPPC QGFSTYGQRR DDDARNQLYV
110 120 130 140 150
PYFGFVEEFR PKAFLIENVV GLLSMSGGAV LADMVARAEA LGYAADVVTL
160 170 180 190 200
DACEYGVPQH RRRVFIFGAA DGQRIDPPQP SHVNGKRSGV VLNDQPSLFF
210 220 230 240 250
DGPSIQPALT VRDAISDLPD EVLVPRDTQK PMEYPEPPKT EYQRLMRGNS
260 270 280 290 300
TELTHHSAKR MLGIRRLRLA MLHPGDYGTK IEERLADGGL NDELIDLMMG
310 320 330 340 350
GAGMRDAAEC RTQDREKEAA LREVLKGGHT TPAKVMEFLD SQGFANKYRR
360 370 380 390 400
LRWDAPSHTV VAHMARDCSD FVHPGIDRFV SVREAARFQS FPDTYRFPGS
410 420
QFRQFRQIGN AVPPLLGRAM AETIKVAIS
Length:429
Mass (Da):47,359
Last modified:May 1, 1997 - v1
Checksum:i97C5EC5414B02FD3
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D78259 Genomic DNA. Translation: BAA11333.1.
AF247972 Genomic DNA. Translation: AAF71525.1.
PIRiJC4656.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D78259 Genomic DNA. Translation: BAA11333.1 .
AF247972 Genomic DNA. Translation: AAF71525.1 .
PIRi JC4656.

3D structure databases

ProteinModelPortali P94147.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 3276. M.AgeI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
InterProi IPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
PANTHERi PTHR10629. PTHR10629. 1 hit.
Pfami PF00145. DNA_methylase. 1 hit.
[Graphical view ]
PRINTSi PR00105. C5METTRFRASE.
SUPFAMi SSF53335. SSF53335. 2 hits.
TIGRFAMsi TIGR00675. dcm. 1 hit.
PROSITEi PS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and nucleotide sequence of the AgeI methylase gene from Agrobacterium gelatinovorum IAM 12617, a marine bacterium."
    Suzuki T., Sugimoto E., Tahara Y., Yamada Y.
    Biosci. Biotechnol. Biochem. 60:444-447(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 25655 / DSM 5887 / IAM 12617 / JCM 20688 / NBRC 15761 / NCIMB / B6.
  2. "Method for cloning and producing AgeI restriction endonuclease in E.coli."
    Xu S.-Y., Maunus R.E., Lunnen K.D., Allen R.
    Patent number EP0959131, 26-APR-1999
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 25655 / DSM 5887 / IAM 12617 / JCM 20688 / NBRC 15761 / NCIMB / B6.

Entry informationi

Entry nameiMTA1_THAGE
AccessioniPrimary (citable) accession number: P94147
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 8, 2000
Last sequence update: May 1, 1997
Last modified: October 1, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3