P94136 (TFDE2_CUPPJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Carboxymethylenebutenolidase 2
EC=3.1.1.45

Alternative name(s):
Carboxymethylenebutenolidase II
Dienelactone hydrolase II
Short name=DLH II

Gene names

Name:	tfdEII
Ordered Locus Names:	Reut_D6472

Encoded on

Plasmid pJP4 Ref.1 Ref.2
Plasmid pReut1 Ref.3

Organism

Cupriavidus pinatubonensis (strain JMP134 / LMG 1197) (Alcaligenes eutrophus) (Ralstonia eutropha) [Complete proteome] [HAMAP]

Taxonomic identifier

264198 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus

Protein attributes

Sequence length	235 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Ring cleavage of cyclic ester dienelactone to produce maleylacetate.
Catalytic activity	4-carboxymethylenebut-2-en-4-olide + H2O = 4-oxohex-2-enedioate.
Pathway	Aromatic compound metabolism; 3-chlorocatechol degradation.
Subunit structure	Monomer By similarity.
Miscellaneous	Carboxymethylenebutenolidase is specific for dienelactone and has no activity toward enol-lactones By similarity.
Sequence similarities	Belongs to the dienelactone hydrolase family.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Molecular function	Hydrolase Serine esterase
Technical term	Complete proteome Plasmid
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	carboxylesterase activity Inferred from electronic annotation. Source: UniProtKB-KW carboxymethylenebutenolidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 235	235	Carboxymethylenebutenolidase 2		PRO_0000161575
Sites
	Active site	117	1	By similarity
	Active site	173	1	By similarity
	Active site	204	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P94136 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 3129DDA5AE48E9F3	FASTA	235	25,400
	10 20 30 40 50 60 MCHDTAPALF PRTASTGSID GAICALCYAG ATRGPRLLVL PDIYGCNAFY RGYAAYLAEQ 70 80 90 100 110 120 GAGEVLLVDP FAAFGELATV TREAAFQRRH RLADRAYVEE LIDFIDGQRI EGVVGFCLGG 130 140 150 160 170 180 LFVFELARQQ VVSRLVAYYP FPQGLENRDP LDVPFDYLPA LRSRHTVIVG DDDALLGTQN 190 200 210 220 230 LQRLQAQARA NDAIDLHIMN GAGHGFLADL ESPDTARAAV AKRGLRIGTT TLLGG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	van der Meer J.R. Submitted (NOV-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Genetic organization of the catabolic plasmid pJP4 from Ralstonia eutropha JMP134 (pJP4) reveals mechanisms of adaptation to chloroaromatic pollutants and evolution of specialized chloroaromatic degradation pathways." Trefault N., De la Iglesia R., Molina A.M., Manzano M., Ledger T., Perez-Pantoja D., Sanchez M.A., Stuardo M., Gonzalez B. Environ. Microbiol. 6:655-668(2004) [PubMed: 15186344] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Complete sequence of plasmid 1 of Ralstonia eutropha JMP134." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Goltsman E., Martinez M., Schmutz J., Larimer F., Land M., Lykidis A., Richardson P. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JMP134 / LMG 1197.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U16782 Genomic DNA. Translation: AAC44728.1. AY365053 Genomic DNA. Translation: AAR31045.1. CP000093 Genomic DNA. Translation: AAZ65770.1.
RefSeq	YP_025393.1. NC_005912.1. YP_293627.1. NC_007337.1.
3D structure databases
ProteinModelPortal	P94136.
ModBase	Search...
Protein-protein interaction databases
STRING	P94136.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2847410. 3607896.
GenomeReviews	Gene locus Reut_D6472 in contig CP000093_GR.
KEGG	reu:Reut_D6472.
PATRIC	20225324. VBIRalEut24049_0541.
Organism-specific databases
CMR	Search...
Phylogenomic databases
ProtClustDB	CLSK757840.
Enzyme and pathway databases
BioCyc	REUT264198:REUT_D6472-MONOMER.
Family and domain databases
InterPro	IPR002925. Dienelactn_hydro. [Graphical view]
KO	K01061.
Pfam	PF01738. DLH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TFDE2_CUPPJ

Accession

Primary (citable) accession number: P94136
Secondary accession number(s): Q46M60

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	May 1, 1997
Last modified:	January 25, 2012
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program