P94135 (TFDF2_CUPPJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Maleylacetate reductase 2
EC=1.3.1.32

Alternative name(s):
Chloromaleylacetate reductase
Maleylacetate reductase II

Gene names

Name:	tfdFII
Ordered Locus Names:	Reut_D6471

Encoded on

Plasmid pJP4 Ref.1 Ref.2 Ref.4
Plasmid pReut1 Ref.3

Organism

Cupriavidus pinatubonensis (strain JMP134 / LMG 1197) (Alcaligenes eutrophus) (Ralstonia eutropha) [Complete proteome] [HAMAP]

Taxonomic identifier

264198 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus › Cupriavidus pinatubonensis

Protein attributes

Sequence length	359 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a major role in the degradation of chloroaromatic compounds by channeling maleylacetate and some of its substituted derivatives into the 3-oxoadipate pathway. This enzyme converts maleylacetate and 2-chloromaleylacetate with similar efficiencies. NADH is preferred to NADPH as the cosubstrate.
Catalytic activity	3-oxoadipate + NAD(P)+ = 2-maleylacetate + NAD(P)H.
Enzyme regulation	Inhibited by p-chloromercuribenzoate and by 3-oxoadipate, and, in a temperature-dependent manner, by manganese.
Pathway	Aromatic compound metabolism; 3-chlorocatechol degradation.
Subunit structure	Homodimer Probable.
Sequence similarities	Belongs to the iron-containing alcohol dehydrogenase family.
Biophysicochemical properties	pH dependence: Optimum pH is 7.5.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing Plasmid
Gene Ontology (GO)
Biological_process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	maleylacetate reductase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 359	359	Maleylacetate reductase 2		PRO_0000087852

Sequences

	Sequence		Length	Mass (Da)
	P94135 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: FC951B482B2575BF	FASTA	359	37,504
	10 20 30 40 50 60 MTGDLNEFVA HFWPVRVVFG AGSTERIPAE VKRLGARRAL VLCTPDQRDL AQRVLGDLGD 70 80 90 100 110 120 LGAGFHDGAV MHVPEASVTR AAQAARDADA DLLVAVGGGS TIGLAKALAL HHGMRFVALP 130 140 150 160 170 180 TTYAGSEMTP IWGLTADGAK RTGRDPRVLP STVLYDPHHL TSLPPEVTGP SGMNAIAHAV 190 200 210 220 230 240 ESMYAPDRNP ITMLLAEESI RAMAQGLPVA VDSPGDLDAR TRTLYAAWLA GTVLGMVSMG 250 260 270 280 290 300 LHHKLCHVLG GRFNLPHAPM HAVLLPHVAA FNEVAAPAEL GRVAAALGAP GPGGAGAALH 310 320 330 340 350 ALLRFTCTER SLAAIGMPAQ GIYDAAEHAL ADAYANPRQA SREDIARLLR AAFTGEMPA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	van der Meer J.R. Submitted (NOV-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Genetic organization of the catabolic plasmid pJP4 from Ralstonia eutropha JMP134 (pJP4) reveals mechanisms of adaptation to chloroaromatic pollutants and evolution of specialized chloroaromatic degradation pathways." Trefault N., De la Iglesia R., Molina A.M., Manzano M., Ledger T., Perez-Pantoja D., Sanchez M.A., Stuardo M., Gonzalez B. Environ. Microbiol. 6:655-668(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Complete sequence of plasmid 1 of Ralstonia eutropha JMP134." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Goltsman E., Martinez M., Schmutz J., Larimer F., Land M., Lykidis A., Richardson P. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JMP134 / LMG 1197.
[4]	"Purification and characterization of maleylacetate reductase from Alcaligenes eutrophus JMP134(pJP4)." Seibert V., Stadler-Fritzsche K., Schlomann M. J. Bacteriol. 175:6745-6754(1993) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-32, CHARACTERIZATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U16782 Genomic DNA. Translation: AAC44727.1. AY365053 Genomic DNA. Translation: AAR31044.1. CP000093 Genomic DNA. Translation: AAZ65769.1.
RefSeq	YP_025392.1. NC_005912.1. YP_293626.1. NC_007337.1.
3D structure databases
ProteinModelPortal	P94135.
ModBase	Search...
Protein-protein interaction databases
STRING	264198.Reut_D6471.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAZ65769; AAZ65769; Reut_D6471.
GeneID	2847409. 3607895.
KEGG	reu:Reut_D6471.
PATRIC	20225322. VBIRalEut24049_0540.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1454.
HOGENOM	HOG000243333.
OMA	AGTISNI.
ProtClustDB	CLSK881605.
Enzyme and pathway databases
BioCyc	CPIN264198:GIW3-6557-MONOMER.
UniPathway	UPA00083.
Family and domain databases
InterPro	IPR001670. ADH_Fe. [Graphical view]
Pfam	PF00465. Fe-ADH. 1 hit. [Graphical view]
PROSITE	PS00913. ADH_IRON_1. False negative. PS00060. ADH_IRON_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TFDF2_CUPPJ

Accession

Primary (citable) accession number: P94135
Secondary accession number(s): Q46M61

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	May 1, 1997
Last modified:	May 1, 2013
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program