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P94126 (GLNA_AZOC5) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamine synthetase

EC=6.3.1.2
Alternative name(s):
Glutamate--ammonia ligase
Gene names
Name:glnA
Ordered Locus Names:AZC_1601
OrganismAzorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / ORS 571) [Complete proteome] [HAMAP]
Taxonomic identifier438753 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesXanthobacteraceaeAzorhizobium

Protein attributes

Sequence length469 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

Enzyme regulation

The activity of this enzyme is controlled by adenylation under conditions of abundant glutamine. The fully adenylated enzyme complex is inactive By similarity.

Subunit structure

Oligomer of 12 subunits arranged in the form of two hexagons By similarity.

Subcellular location

Cytoplasm.

Sequence similarities

Belongs to the glutamine synthetase family.

Ontologies

Keywords
   Biological processNitrogen fixation
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processglutamine biosynthetic process

Inferred from electronic annotation. Source: InterPro

nitrogen fixation

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

glutamate-ammonia ligase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 469469Glutamine synthetase
PRO_0000153230

Amino acid modifications

Modified residue3981O-AMP-tyrosine By similarity

Experimental info

Sequence conflict58 – 603GWK → AE in CAA71265. Ref.1
Sequence conflict2791G → R in CAA71265. Ref.1
Sequence conflict4131G → C in CAA71265. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P94126 [UniParc].

Last modified February 26, 2008. Version 2.
Checksum: 302A2DA5C9DB35CA

FASTA46952,386
        10         20         30         40         50         60 
MKTAKEVLDF IKSNDVKYVD LRFTDPRGKW QHVTFDITMV DEDFFAEGQA FDGSSIAGWK 

        70         80         90        100        110        120 
AINESDMLLM PDVTTACVDP FFSETTLSVV CDVLEPTTGE PYGRDPRGIA KKAMAYLQST 

       130        140        150        160        170        180 
GIGDTVFFGP EAEFFIFDDV KFKADPYNTG FKLDSIELPT NGDTDYEGGN LGHRIKTKGG 

       190        200        210        220        230        240 
YFPVPPLDSA QDMRSEMLAS MAKMGAKVEK HHHEVASAQH ELGLKFGQLV TMADHLQVYK 

       250        260        270        280        290        300 
YCIHQVANIY GKTATFMPKP VYGDNGSGMH VHQSIWKDGK PLFAGDKYAD LSQECLWYIG 

       310        320        330        340        350        360 
GVIKHAKSLN AFTNPLTNSY KRLVPGYEAP VLLAYSARNR SASCRIPYTN NPKAKRVEVR 

       370        380        390        400        410        420 
FPDPGANPYL AFSALFMAGM DGILNKIDPG SAMDKDLYDL PPAELKQIPT VCGSLREALE 

       430        440        450        460 
SLAKDHDYLL KGDVFQKDFI ESYIDLKMQE VARFEMTPHP VEFEMYYSV 

« Hide

References

« Hide 'large scale' references
[1]"Characterization of Azorhizobium caulinodans glnB and glnA genes: involvement of the P(II) protein in symbiotic nitrogen fixation."
Michel-Reydellet N., Desnoues N., Elmerich C., Kaminski P.A.
J. Bacteriol. 179:3580-3587(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Complete genome sequence of the nitrogen-fixing bacterium Azorhizobium caulinodans ORS571."
Lee K.B., Backer P.D., Aono T., Liu C.T., Suzuki S., Suzuki T., Kaneko T., Yamada M., Tabata S., Kupfer D.M., Najar F.Z., Wiley G.B., Roe B., Binnewies T., Ussery D., Vereecke D., Gevers D., Holsters M., Oyaizu H.
Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 43989 / DSM 5975 / ORS 571.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Y10213 Genomic DNA. Translation: CAA71265.1.
AP009384 Genomic DNA. Translation: BAF87599.1.
RefSeqYP_001524517.1. NC_009937.1.

3D structure databases

ProteinModelPortalP94126.
SMRP94126. Positions 3-469.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING438753.AZC_1601.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAF87599; BAF87599; AZC_1601.
GeneID5691233.
KEGGazc:AZC_1601.
PATRIC21019821. VBIAzoCau17976_1676.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0174.
HOGENOMHOG000005157.
KOK01915.
OMAQKYKYVI.
OrthoDBEOG6B360N.

Enzyme and pathway databases

BioCycACAU438753:GJF3-1620-MONOMER.

Family and domain databases

Gene3D3.10.20.70. 1 hit.
3.30.590.10. 1 hit.
InterProIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR004809. Gln_synth_I.
IPR001637. Gln_synth_I_adenylation_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMSSF54368. SSF54368. 1 hit.
TIGRFAMsTIGR00653. GlnA. 1 hit.
PROSITEPS00180. GLNA_1. 1 hit.
PS00182. GLNA_ADENYLATION. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGLNA_AZOC5
AccessionPrimary (citable) accession number: P94126
Secondary accession number(s): A8HYD0
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: February 26, 2008
Last modified: May 14, 2014
This is version 79 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families