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Protein

Glutamine synthetase

Gene

glnA

Organism
Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / JCM 20966 / NBRC 14845 / NCIMB 13405 / ORS 571)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the ATP-dependent biosynthesis of glutamine from glutamate and ammonia.By similarity

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.By similarity

Cofactori

Mg2+By similarityNote: Binds 2 Mg2+ ions per subunit.By similarity

Enzyme regulationi

The activity of this enzyme could be controlled by adenylation under conditions of abundant glutamine.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi131Magnesium 1By similarity1
Metal bindingi133Magnesium 2By similarity1
Binding sitei209ATPBy similarity1
Metal bindingi214Magnesium 2By similarity1
Metal bindingi221Magnesium 2By similarity1
Binding sitei266L-glutamate; via carbonyl oxygenBy similarity1
Metal bindingi270Magnesium 1; via pros nitrogenBy similarity1
Binding sitei274ATPBy similarity1
Binding sitei322L-glutamateBy similarity1
Binding sitei328L-glutamateBy similarity1
Binding sitei340ATPBy similarity1
Binding sitei340L-glutamateBy similarity1
Binding sitei345ATPBy similarity1
Binding sitei353ATPBy similarity1
Metal bindingi358Magnesium 1By similarity1
Binding sitei360L-glutamateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi272 – 274ATPBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processNitrogen fixation
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciACAU438753:GJF3-1620-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetaseBy similarity (EC:6.3.1.2By similarity)
Short name:
GSBy similarity
Alternative name(s):
Glutamate--ammonia ligaseCurated
Glutamine synthetase I betaBy similarity
Short name:
GSI betaBy similarity
Gene namesi
Name:glnABy similarity
Ordered Locus Names:AZC_1601
OrganismiAzorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / JCM 20966 / NBRC 14845 / NCIMB 13405 / ORS 571)
Taxonomic identifieri438753 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesXanthobacteraceaeAzorhizobium
Proteomesi
  • UP000000270 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001532301 – 469Glutamine synthetaseAdd BLAST469

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei398O-AMP-tyrosineBy similarity1

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Oligomer of 12 subunits arranged in the form of two hexameric ring.By similarity

Protein-protein interaction databases

STRINGi438753.AZC_1601.

Structurei

3D structure databases

ProteinModelPortaliP94126.
SMRiP94126.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni265 – 266L-glutamate bindingBy similarity2

Sequence similaritiesi

Belongs to the glutamine synthetase family.Curated

Phylogenomic databases

eggNOGiENOG4105C5F. Bacteria.
COG0174. LUCA.
HOGENOMiHOG000005157.
KOiK01915.
OMAiKVLNQVG.
OrthoDBiPOG091H02IE.

Family and domain databases

Gene3Di3.10.20.70. 1 hit.
3.30.590.10. 1 hit.
InterProiView protein in InterPro
IPR008147. Gln_synt_b-grasp.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR004809. Gln_synth_I.
IPR001637. Gln_synth_I_adenylation_site.
IPR027302. Gln_synth_N_conserv_site.
PfamiView protein in Pfam
PF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
SMARTiView protein in SMART
SM01230. Gln-synt_C. 1 hit.
SUPFAMiSSF54368. SSF54368. 1 hit.
TIGRFAMsiTIGR00653. GlnA. 1 hit.
PROSITEiView protein in PROSITE
PS00180. GLNA_1. 1 hit.
PS00182. GLNA_ADENYLATION. 1 hit.
PS00181. GLNA_ATP. 1 hit.

Sequencei

Sequence statusi: Complete.

P94126-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTAKEVLDF IKSNDVKYVD LRFTDPRGKW QHVTFDITMV DEDFFAEGQA
60 70 80 90 100
FDGSSIAGWK AINESDMLLM PDVTTACVDP FFSETTLSVV CDVLEPTTGE
110 120 130 140 150
PYGRDPRGIA KKAMAYLQST GIGDTVFFGP EAEFFIFDDV KFKADPYNTG
160 170 180 190 200
FKLDSIELPT NGDTDYEGGN LGHRIKTKGG YFPVPPLDSA QDMRSEMLAS
210 220 230 240 250
MAKMGAKVEK HHHEVASAQH ELGLKFGQLV TMADHLQVYK YCIHQVANIY
260 270 280 290 300
GKTATFMPKP VYGDNGSGMH VHQSIWKDGK PLFAGDKYAD LSQECLWYIG
310 320 330 340 350
GVIKHAKSLN AFTNPLTNSY KRLVPGYEAP VLLAYSARNR SASCRIPYTN
360 370 380 390 400
NPKAKRVEVR FPDPGANPYL AFSALFMAGM DGILNKIDPG SAMDKDLYDL
410 420 430 440 450
PPAELKQIPT VCGSLREALE SLAKDHDYLL KGDVFQKDFI ESYIDLKMQE
460
VARFEMTPHP VEFEMYYSV
Length:469
Mass (Da):52,386
Last modified:February 26, 2008 - v2
Checksum:i302A2DA5C9DB35CA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti58 – 60GWK → AE in CAA71265 (PubMed:9171403).Curated3
Sequence conflicti279G → R in CAA71265 (PubMed:9171403).Curated1
Sequence conflicti413G → C in CAA71265 (PubMed:9171403).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y10213 Genomic DNA. Translation: CAA71265.1.
AP009384 Genomic DNA. Translation: BAF87599.1.
RefSeqiWP_012170129.1. NC_009937.1.

Genome annotation databases

EnsemblBacteriaiBAF87599; BAF87599; AZC_1601.
KEGGiazc:AZC_1601.
PATRICi21019821. VBIAzoCau17976_1676.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y10213 Genomic DNA. Translation: CAA71265.1.
AP009384 Genomic DNA. Translation: BAF87599.1.
RefSeqiWP_012170129.1. NC_009937.1.

3D structure databases

ProteinModelPortaliP94126.
SMRiP94126.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi438753.AZC_1601.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAF87599; BAF87599; AZC_1601.
KEGGiazc:AZC_1601.
PATRICi21019821. VBIAzoCau17976_1676.

Phylogenomic databases

eggNOGiENOG4105C5F. Bacteria.
COG0174. LUCA.
HOGENOMiHOG000005157.
KOiK01915.
OMAiKVLNQVG.
OrthoDBiPOG091H02IE.

Enzyme and pathway databases

BioCyciACAU438753:GJF3-1620-MONOMER.

Family and domain databases

Gene3Di3.10.20.70. 1 hit.
3.30.590.10. 1 hit.
InterProiView protein in InterPro
IPR008147. Gln_synt_b-grasp.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR004809. Gln_synth_I.
IPR001637. Gln_synth_I_adenylation_site.
IPR027302. Gln_synth_N_conserv_site.
PfamiView protein in Pfam
PF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
SMARTiView protein in SMART
SM01230. Gln-synt_C. 1 hit.
SUPFAMiSSF54368. SSF54368. 1 hit.
TIGRFAMsiTIGR00653. GlnA. 1 hit.
PROSITEiView protein in PROSITE
PS00180. GLNA_1. 1 hit.
PS00182. GLNA_ADENYLATION. 1 hit.
PS00181. GLNA_ATP. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGLN1B_AZOC5
AccessioniPrimary (citable) accession number: P94126
Secondary accession number(s): A8HYD0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: February 26, 2008
Last modified: April 12, 2017
This is version 96 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.