Reviewed, UniProtKB/Swiss-Prot P94111 (STS1_ARATH)

Names and origin

Protein names

Recommended name:
    Strictosidine synthase 1
      Short name=SS-1
    EC=4.3.3.2

Gene names

Name:	SS1
Ordered Locus Names:	At1g74020
ORF Names:	F2P9.11

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	335 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the stereospecific condensation of tryptamine with secologanin to form strictosidine, the key intermediate of indole alkaloid biosynthesis By similarity.
Catalytic activity	3-alpha-(S)-strictosidine + H2O = tryptamine + secologanin.
Pathway	Alkaloid biosynthesis; 3alpha(S)-strictosidine biosynthesis; 3alpha(S)-strictosidine from secologanin and tryptamine: step 1/1.
Subcellular location	Vacuole By similarity.
Sequence similarities	Belongs to the strictosidine synthase family.

Ontologies

Keywords
Biological process	Alkaloid metabolism
Cellular component	Vacuole
Domain	Signal
Molecular function	Lyase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alkaloid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW biosynthetic process Inferred from electronic annotation. Source: InterPro response to jasmonic acid stimulus Inferred from expression pattern. Source: TAIR response to wounding Inferred from expression pattern. Source: TAIR
Cellular component	plasma membrane Inferred from direct assay. Source: TAIR vacuole Inferred from direct assay. Source: TAIR
Molecular function	strictosidine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 335	313	Strictosidine synthase 1		PRO_0000033330
Amino acid modifications
	Glycosylation	80	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	19	1	A → P in AAB40594. Ref.1
	Sequence conflict	19	1	A → P in AAB40593. Ref.1
	Sequence conflict	327	1	L → I in AAB40594. Ref.1
	Sequence conflict	327	1	L → I in AAB40593. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P94111-1 [UniParc]. Last modified June 6, 2002. Version 2. Checksum: C1382BF040A72849	FASTA	335	35,293
	10 20 30 40 50 60 MTSFCSMISL LLLLSLSSAV FSDDASFQKL PVPETRSGPE AFAFDSTGKG FYTGVSGGKI 70 80 90 100 110 120 LKYLPETGYV DFAQITESSN SSWCDGTIGT ALAGRCGRPA GIAFNEKTGD LYVADAPLGL 130 140 150 160 170 180 HVISPAGGLA TKITDSVDGK PFKFLDGLDV DPTTGVVYFT SFSSRFSPIQ VLIALGLKDA 190 200 210 220 230 240 TGKLYKYDPS TKVVTVLMEG LSGSAGCAVS SDGSFVLVSQ FTKSNIKRYW IKGPKAGSSE 250 260 270 280 290 300 DFTNSVSNPD NIKRIGSTGN FWVASVVNKI IVPTNPSAVK VNSNGEVLQT IPLKDKFGDT 310 320 330 LLSEVNEFEG NLYIGTLTGP FAGILKLEKG SCPAT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Jain A.K., Nessler C.L. Submitted (DEC-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Strain: cv. Landsberg erecta.
[2]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U43945 mRNA. Translation: AAB40594.1. U43713 Genomic DNA. Translation: AAB40593.1. AC016662 Genomic DNA. Translation: AAG52513.1. AY034970 mRNA. Translation: AAK59475.1. AY062976 mRNA. Translation: AAL34150.1.
IPI	IPI00544988.
PIR	A96768.
RefSeq	NP_177542.1.
UniGene	At.24431
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	P94111.
Genome annotation databases
GeneID	843740.
GenomeReviews	Gene locus AT1G74020 in contig CT485782_GR.
KEGG	ath:AT1G74020.
NMPDR	fig|3702.1.peg.6910.
Organism-specific databases
TAIR	At1g74020.
Phylogenomic databases
OMA	GANSANF.
Enzyme and pathway databases
BRENDA	4.3.3.2. 302.
Gene expression databases
ArrayExpress	P94111.
Genevestigator	P94111.
GermOnline	AT1G74020. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR011042. 6-blade_b-propeller_TolB-like. IPR018119. Strictosidine_synth_cons-reg. IPR004141. Strictosidine_synthase. [Graphical view]
Gene3D	G3DSA:2.120.10.30. 6-blade_b-propeller_TolB-like. 1 hit.
PANTHER	PTHR10426. Str_synth. 1 hit.
Pfam	PF03088. Str_synth. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

STS1_ARATH

Accession

Primary (citable) accession number: P94111
Secondary accession number(s): Q9C9C3

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	June 6, 2002
Last modified:	November 3, 2009
This is version 65 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)