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Protein

Protein STRICTOSIDINE SYNTHASE-LIKE 12

Gene

SSL12

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the stereospecific condensation of tryptamine with secologanin to form strictosidine, the key intermediate of indole alkaloid biosynthesis.By similarity

Catalytic activityi

3-alpha-(S)-strictosidine + H2O = tryptamine + secologanin.

Pathway: 3alpha(S)-strictosidine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 3alpha(S)-strictosidine from secologanin and tryptamine.
Proteins known to be involved in this subpathway in this organism are:
  1. Protein STRICTOSIDINE SYNTHASE-LIKE 11 (SSL11), Protein STRICTOSIDINE SYNTHASE-LIKE 12 (SSL12)
This subpathway is part of the pathway 3alpha(S)-strictosidine biosynthesis, which is itself part of Alkaloid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 3alpha(S)-strictosidine from secologanin and tryptamine, the pathway 3alpha(S)-strictosidine biosynthesis and in Alkaloid biosynthesis.

GO - Molecular functioni

GO - Biological processi

  • alkaloid metabolic process Source: UniProtKB-KW
  • biosynthetic process Source: InterPro
  • cellular response to potassium ion starvation Source: UniProtKB
  • response to jasmonic acid Source: TAIR
  • response to wounding Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Alkaloid metabolism

Enzyme and pathway databases

BioCyciARA:AT1G74020-MONOMER.
UniPathwayiUPA00311; UER00447.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein STRICTOSIDINE SYNTHASE-LIKE 121 Publication (EC:4.3.3.2)
Short name:
AtSSL121 Publication
Alternative name(s):
Strictosidine synthase 1
Short name:
SS-1
Strictosidine synthase 131 Publication
Short name:
AtSS131 Publication
Gene namesi
Name:SSL121 Publication
Synonyms:SS1, SS131 Publication
Ordered Locus Names:At1g74020Imported
ORF Names:F2P9.11Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G74020.

Subcellular locationi

GO - Cellular componenti

  • Golgi apparatus Source: TAIR
  • plasma membrane Source: TAIR
  • plasmodesma Source: TAIR
  • vacuolar membrane Source: TAIR
  • vacuole Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence AnalysisAdd
BLAST
Chaini23 – 335313Protein STRICTOSIDINE SYNTHASE-LIKE 12PRO_0000033330Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi80 – 801N-linked (GlcNAc...)PROSITE-ProRule annotation

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiP94111.
PRIDEiP94111.

Expressioni

Inductioni

Upon potassium (K) starvation.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi3702.AT1G74020.1.

Structurei

3D structure databases

ProteinModelPortaliP94111.
SMRiP94111. Positions 38-325.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the strictosidine synthase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG3386.
HOGENOMiHOG000223915.
KOiK01757.
OMAiEVNEFEG.
PhylomeDBiP94111.

Family and domain databases

Gene3Di2.120.10.30. 1 hit.
InterProiIPR011042. 6-blade_b-propeller_TolB-like.
IPR018119. Strictosidine_synth_cons-reg.
IPR004141. Strictosidine_synthase.
[Graphical view]
PANTHERiPTHR10426:SF22. PTHR10426:SF22. 1 hit.
PfamiPF03088. Str_synth. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P94111-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSFCSMISL LLLLSLSSAV FSDDASFQKL PVPETRSGPE AFAFDSTGKG
60 70 80 90 100
FYTGVSGGKI LKYLPETGYV DFAQITESSN SSWCDGTIGT ALAGRCGRPA
110 120 130 140 150
GIAFNEKTGD LYVADAPLGL HVISPAGGLA TKITDSVDGK PFKFLDGLDV
160 170 180 190 200
DPTTGVVYFT SFSSRFSPIQ VLIALGLKDA TGKLYKYDPS TKVVTVLMEG
210 220 230 240 250
LSGSAGCAVS SDGSFVLVSQ FTKSNIKRYW IKGPKAGSSE DFTNSVSNPD
260 270 280 290 300
NIKRIGSTGN FWVASVVNKI IVPTNPSAVK VNSNGEVLQT IPLKDKFGDT
310 320 330
LLSEVNEFEG NLYIGTLTGP FAGILKLEKG SCPAT
Length:335
Mass (Da):35,293
Last modified:June 6, 2002 - v2
Checksum:iC1382BF040A72849
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti19 – 191A → P in AAB40594 (Ref. 1) Curated
Sequence conflicti19 – 191A → P in AAB40593 (Ref. 1) Curated
Sequence conflicti327 – 3271L → I in AAB40594 (Ref. 1) Curated
Sequence conflicti327 – 3271L → I in AAB40593 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43945 mRNA. Translation: AAB40594.1.
U43713 Genomic DNA. Translation: AAB40593.1.
AC016662 Genomic DNA. Translation: AAG52513.1.
CP002684 Genomic DNA. Translation: AEE35538.1.
AY034970 mRNA. Translation: AAK59475.1.
AY062976 mRNA. Translation: AAL34150.1.
PIRiA96768.
RefSeqiNP_177542.1. NM_106061.3.
UniGeneiAt.24431.
At.74858.

Genome annotation databases

EnsemblPlantsiAT1G74020.1; AT1G74020.1; AT1G74020.
GeneIDi843740.
KEGGiath:AT1G74020.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43945 mRNA. Translation: AAB40594.1.
U43713 Genomic DNA. Translation: AAB40593.1.
AC016662 Genomic DNA. Translation: AAG52513.1.
CP002684 Genomic DNA. Translation: AEE35538.1.
AY034970 mRNA. Translation: AAK59475.1.
AY062976 mRNA. Translation: AAL34150.1.
PIRiA96768.
RefSeqiNP_177542.1. NM_106061.3.
UniGeneiAt.24431.
At.74858.

3D structure databases

ProteinModelPortaliP94111.
SMRiP94111. Positions 38-325.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT1G74020.1.

Proteomic databases

PaxDbiP94111.
PRIDEiP94111.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G74020.1; AT1G74020.1; AT1G74020.
GeneIDi843740.
KEGGiath:AT1G74020.

Organism-specific databases

TAIRiAT1G74020.

Phylogenomic databases

eggNOGiCOG3386.
HOGENOMiHOG000223915.
KOiK01757.
OMAiEVNEFEG.
PhylomeDBiP94111.

Enzyme and pathway databases

UniPathwayiUPA00311; UER00447.
BioCyciARA:AT1G74020-MONOMER.

Miscellaneous databases

PROiP94111.

Family and domain databases

Gene3Di2.120.10.30. 1 hit.
InterProiIPR011042. 6-blade_b-propeller_TolB-like.
IPR018119. Strictosidine_synth_cons-reg.
IPR004141. Strictosidine_synthase.
[Graphical view]
PANTHERiPTHR10426:SF22. PTHR10426:SF22. 1 hit.
PfamiPF03088. Str_synth. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Jain A.K., Nessler C.L.
    Submitted (DEC-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Strain: cv. Landsberg erecta.
  2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Animal and plant members of a gene family with similarity to alkaloid-synthesizing enzymes."
    Fabbri M., Delp G., Schmidt O., Theopold U.
    Biochem. Biophys. Res. Commun. 271:191-196(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  6. Cited for: INDUCTION BY POTASSIUM STARVATION.
    Strain: cv. Wassilewskija-2.
  7. "Phylogenetic and transcriptional analysis of a strictosidine synthase-like gene family in Arabidopsis thaliana reveals involvement in plant defence responses."
    Sohani M.M., Schenk P.M., Schultz C.J., Schmidt O.
    Plant Biol. 11:105-117(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY.
    Strain: cv. Columbia.

Entry informationi

Entry nameiSSL12_ARATH
AccessioniPrimary (citable) accession number: P94111
Secondary accession number(s): Q9C9C3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: June 6, 2002
Last modified: June 24, 2015
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.