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Protein

DNA repair protein RAD51 homolog 1

Gene

RAD51

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Unwinds duplex DNA (By similarity). Component of the meiotic recombination pathway. Seems to play a role in mediating chromosome homology search, chromosome pairing and synapsis at early stages and probably chromosome crossing-over at later stages in meiosis. Probably is involved in the repair of meiotic double strand breaks (DBSs) generated by AtSPO11-1 and in homologous recombination. Its function is dispensable for vegetative growth and root mitosis.By similarity1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi130 – 137ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

  • chromosome organization involved in meiotic cell cycle Source: GO_Central
  • DNA metabolic process Source: TAIR
  • DNA recombinase assembly Source: GO_Central
  • double-strand break repair Source: TAIR
  • double-strand break repair via synthesis-dependent strand annealing Source: TAIR
  • mitotic recombination Source: GO_Central
  • mitotic recombination-dependent replication fork processing Source: InterPro
  • reciprocal meiotic recombination Source: GO_Central
  • regulation of transcription, DNA-templated Source: TAIR
  • response to gamma radiation Source: TAIR
  • response to radiation Source: TAIR
  • strand invasion Source: GO_Central

Keywordsi

Molecular functionDNA-binding
Biological processDNA damage, DNA recombination, DNA repair
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-5693616 Presynaptic phase of homologous DNA pairing and strand exchange

Names & Taxonomyi

Protein namesi
Recommended name:
DNA repair protein RAD51 homolog 11 Publication
Alternative name(s):
Rad51-like protein 11 Publication
Short name:
AtRAD511 Publication
Gene namesi
Name:RAD511 Publication
Ordered Locus Names:At5g20850Imported
ORF Names:F22D1.20Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G20850
TAIRilocus:2147092 AT5G20850

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001229261 – 342DNA repair protein RAD51 homolog 1Add BLAST342

Proteomic databases

PaxDbiP94102

Expressioni

Tissue specificityi

Detected in various tissues. Higher expression in reproductive tissues than in vegetative tissues, with the highest expression level in young flower buds. At cellular level, is expressed at low levels in flower primordia, then at higher levels in young anthers and at highest levels in both females and males meiocytes. Not detected in gametophytes.4 Publications

Developmental stagei

Cell cycle regulated, peaking at the S phase. It is also expressed at high levels in exponentially growing cells in suspension cultures.1 Publication

Inductioni

By genotoxic stress and by DNA damage (gamma-radiation, UV-B). Regulated by ATM in response to DNA double strand breaks (DSBs).4 Publications

Gene expression databases

ExpressionAtlasiP94102 baseline and differential
GenevisibleiP94102 AT

Interactioni

Subunit structurei

Self-associates and interacts with XRCC3 (PubMed:12139010). Binds to RAD54/CHR25 (PubMed:17227544). Interacts with BRCA2A and BRCA2B (PubMed:16415210). Can form a tripartite complex with both BRCA2B and DSS1(I) (PubMed:16415210).3 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi17483, 7 interactors
DIPiDIP-31780N
IntActiP94102, 11 interactors
STRINGi3702.AT5G20850.1

Structurei

3D structure databases

ProteinModelPortaliP94102
SMRiP94102
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini51 – 80HhHAdd BLAST30
Domaini100 – 314FtsKPROSITE-ProRule annotationAdd BLAST215

Sequence similaritiesi

Belongs to the RecA family. RAD51 subfamily.Curated

Phylogenomic databases

eggNOGiKOG1433 Eukaryota
COG0468 LUCA
HOGENOMiHOG000227426
InParanoidiP94102
KOiK04482
OMAiYNTDHQT
OrthoDBiEOG09360EJO
PhylomeDBiP94102

Family and domain databases

CDDicd01123 Rad51_DMC1_radA, 1 hit
InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR011941 DNA_recomb/repair_Rad51
IPR013632 DNA_recomb/repair_Rad51_C
IPR016467 DNA_recomb/repair_RecA-like
IPR010995 DNA_repair_Rad51/TF_NusA_a-hlx
IPR027417 P-loop_NTPase
IPR033925 Rad51_DMC1_RadA
IPR020588 RecA_ATP-bd
IPR020587 RecA_monomer-monomer_interface
PfamiView protein in Pfam
PF08423 Rad51, 1 hit
PIRSFiPIRSF005856 Rad51, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SUPFAMiSSF47794 SSF47794, 1 hit
SSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR02239 recomb_RAD51, 1 hit
PROSITEiView protein in PROSITE
PS50162 RECA_2, 1 hit
PS50163 RECA_3, 1 hit

Sequencei

Sequence statusi: Complete.

P94102-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTMEQRRNQ NAVQQQDDEE TQHGPFPVEQ LQAAGIASVD VKKLRDAGLC
60 70 80 90 100
TVEGVAYTPR KDLLQIKGIS DAKVDKIVEA ASKLVPLGFT SASQLHAQRQ
110 120 130 140 150
EIIQITSGSR ELDKVLEGGI ETGSITELYG EFRSGKTQLC HTLCVTCQLP
160 170 180 190 200
MDQGGGEGKA MYIDAEGTFR PQRLLQIADR FGLNGADVLE NVAYARAYNT
210 220 230 240 250
DHQSRLLLEA ASMMIETRFA LLIVDSATAL YRTDFSGRGE LSARQMHLAK
260 270 280 290 300
FLRSLQKLAD EFGVAVVITN QVVAQVDGSA LFAGPQFKPI GGNIMAHATT
310 320 330 340
TRLALRKGRA EERICKVISS PCLPEAEARF QISTEGVTDC KD
Length:342
Mass (Da):37,304
Last modified:May 1, 1997 - v1
Checksum:i08067F8D85A2E77D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43652 Genomic DNA Translation: AAC49555.1
AJ001100 Genomic DNA Translation: CAA04529.1
U43528 mRNA Translation: AAB37762.1
AF296834 Genomic DNA No translation available.
CP002688 Genomic DNA Translation: AED92897.1
RefSeqiNP_568402.1, NM_122092.3
UniGeneiAt.110

Genome annotation databases

EnsemblPlantsiAT5G20850.1; AT5G20850.1; AT5G20850
GeneIDi832208
GrameneiAT5G20850.1; AT5G20850.1; AT5G20850
KEGGiath:AT5G20850

Similar proteinsi

Entry informationi

Entry nameiRAD51_ARATH
AccessioniPrimary (citable) accession number: P94102
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 23, 2004
Last sequence update: May 1, 1997
Last modified: April 25, 2018
This is version 137 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health