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Protein

DNA repair protein RAD51 homolog 1

Gene

RAD51

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Unwinds duplex DNA (By similarity). Component of the meiotic recombination pathway. Seems to play a role in mediating chromosome homology search, chromosome pairing and synapsis at early stages and probably chromosome crossing-over at later stages in meiosis. Probably is involved in the repair of meiotic double strand breaks (DBSs) generated by AtSPO11-1 and in homologous recombination. Its function is dispensable for vegetative growth and root mitosis.By similarity1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi130 – 1378ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • chromosome organization involved in meiotic cell cycle Source: GO_Central
  • DNA metabolic process Source: TAIR
  • DNA recombinase assembly Source: GO_Central
  • double-strand break repair Source: TAIR
  • double-strand break repair via synthesis-dependent strand annealing Source: TAIR
  • homologous recombination-dependent replication fork processing Source: InterPro
  • mitotic recombination Source: GO_Central
  • reciprocal meiotic recombination Source: GO_Central
  • regulation of transcription, DNA-templated Source: TAIR
  • response to gamma radiation Source: TAIR
  • response to radiation Source: TAIR
  • strand invasion Source: GO_Central
Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA repair protein RAD51 homolog 11 Publication
Alternative name(s):
Rad51-like protein 11 Publication
Short name:
AtRAD511 Publication
Gene namesi
Name:RAD511 Publication
Ordered Locus Names:At5g20850Imported
ORF Names:F22D1.20Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G20850.

Subcellular locationi

GO - Cellular componenti

  • chromosome Source: TAIR
  • condensed nuclear chromosome Source: GO_Central
  • nucleoplasm Source: GO_Central
  • nucleus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 342342DNA repair protein RAD51 homolog 1PRO_0000122926Add
BLAST

Proteomic databases

PaxDbiP94102.

Expressioni

Tissue specificityi

Detected in various tissues. Higher expression in reproductive tissues than in vegetative tissues, with the highest expression level in young flower buds. At cellular level, is expressed at low levels in flower primordia, then at higher levels in young anthers and at highest levels in both females and males meiocytes. Not detected in gametophytes.4 Publications

Developmental stagei

Cell cycle regulated, peaking at the S phase. It is also expressed at high levels in exponentially growing cells in suspension cultures.1 Publication

Inductioni

By genotoxic stress and by DNA damage (gamma-radiation, UV-B). Regulated by ATM in response to DNA double strand breaks (DSBs).4 Publications

Gene expression databases

GenevisibleiP94102. AT.

Interactioni

Subunit structurei

Self-associates and interacts with XRCC3 (PubMed:12139010). Binds to RAD54/CHR25 (PubMed:17227544). Interacts with BRCA2A and BRCA2B (PubMed:16415210). Can form a tripartite complex with both BRCA2B and DSS1(I) (PubMed:16415210).3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
BRCA2AQ7Y1C54EBI-307687,EBI-307680
BRCA2BQ7Y1C45EBI-307687,EBI-307707
CHR25Q0PCS33EBI-307687,EBI-1768899
DMC1Q390094EBI-307687,EBI-307715
MND1Q8GYD22EBI-307687,EBI-1554720

Protein-protein interaction databases

BioGridi17483. 7 interactions.
DIPiDIP-31780N.
IntActiP94102. 11 interactions.
STRINGi3702.AT5G20850.1.

Structurei

3D structure databases

ProteinModelPortaliP94102.
SMRiP94102. Positions 27-342.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini51 – 8030HhHAdd
BLAST
Domaini100 – 314215FtsKPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the RecA family. RAD51 subfamily.Curated
Contains 1 FtsK domain.Curated
Contains 1 HhH domain.Curated

Phylogenomic databases

eggNOGiKOG1433. Eukaryota.
COG0468. LUCA.
HOGENOMiHOG000227426.
InParanoidiP94102.
KOiK04482.
OMAiLGRFLRM.
PhylomeDBiP94102.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR011941. DNA_recomb/repair_Rad51.
IPR013632. DNA_recomb/repair_Rad51_C.
IPR016467. DNA_recomb/repair_RecA-like.
IPR010995. DNA_repair_Rad51/TF_NusA_a-hlx.
IPR027417. P-loop_NTPase.
IPR020588. RecA_ATP-bd.
IPR020587. RecA_monomer-monomer_interface.
[Graphical view]
PfamiPF08423. Rad51. 1 hit.
[Graphical view]
PIRSFiPIRSF005856. Rad51. 1 hit.
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF47794. SSF47794. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR02239. recomb_RAD51. 1 hit.
PROSITEiPS50162. RECA_2. 1 hit.
PS50163. RECA_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P94102-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTMEQRRNQ NAVQQQDDEE TQHGPFPVEQ LQAAGIASVD VKKLRDAGLC
60 70 80 90 100
TVEGVAYTPR KDLLQIKGIS DAKVDKIVEA ASKLVPLGFT SASQLHAQRQ
110 120 130 140 150
EIIQITSGSR ELDKVLEGGI ETGSITELYG EFRSGKTQLC HTLCVTCQLP
160 170 180 190 200
MDQGGGEGKA MYIDAEGTFR PQRLLQIADR FGLNGADVLE NVAYARAYNT
210 220 230 240 250
DHQSRLLLEA ASMMIETRFA LLIVDSATAL YRTDFSGRGE LSARQMHLAK
260 270 280 290 300
FLRSLQKLAD EFGVAVVITN QVVAQVDGSA LFAGPQFKPI GGNIMAHATT
310 320 330 340
TRLALRKGRA EERICKVISS PCLPEAEARF QISTEGVTDC KD
Length:342
Mass (Da):37,304
Last modified:May 1, 1997 - v1
Checksum:i08067F8D85A2E77D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43652 Genomic DNA. Translation: AAC49555.1.
AJ001100 Genomic DNA. Translation: CAA04529.1.
U43528 mRNA. Translation: AAB37762.1.
AF296834 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED92897.1.
RefSeqiNP_568402.1. NM_122092.2.
UniGeneiAt.110.

Genome annotation databases

EnsemblPlantsiAT5G20850.1; AT5G20850.1; AT5G20850.
GeneIDi832208.
GrameneiAT5G20850.1; AT5G20850.1; AT5G20850.
KEGGiath:AT5G20850.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43652 Genomic DNA. Translation: AAC49555.1.
AJ001100 Genomic DNA. Translation: CAA04529.1.
U43528 mRNA. Translation: AAB37762.1.
AF296834 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED92897.1.
RefSeqiNP_568402.1. NM_122092.2.
UniGeneiAt.110.

3D structure databases

ProteinModelPortaliP94102.
SMRiP94102. Positions 27-342.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi17483. 7 interactions.
DIPiDIP-31780N.
IntActiP94102. 11 interactions.
STRINGi3702.AT5G20850.1.

Proteomic databases

PaxDbiP94102.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G20850.1; AT5G20850.1; AT5G20850.
GeneIDi832208.
GrameneiAT5G20850.1; AT5G20850.1; AT5G20850.
KEGGiath:AT5G20850.

Organism-specific databases

TAIRiAT5G20850.

Phylogenomic databases

eggNOGiKOG1433. Eukaryota.
COG0468. LUCA.
HOGENOMiHOG000227426.
InParanoidiP94102.
KOiK04482.
OMAiLGRFLRM.
PhylomeDBiP94102.

Miscellaneous databases

PROiP94102.

Gene expression databases

GenevisibleiP94102. AT.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR011941. DNA_recomb/repair_Rad51.
IPR013632. DNA_recomb/repair_Rad51_C.
IPR016467. DNA_recomb/repair_RecA-like.
IPR010995. DNA_repair_Rad51/TF_NusA_a-hlx.
IPR027417. P-loop_NTPase.
IPR020588. RecA_ATP-bd.
IPR020587. RecA_monomer-monomer_interface.
[Graphical view]
PfamiPF08423. Rad51. 1 hit.
[Graphical view]
PIRSFiPIRSF005856. Rad51. 1 hit.
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF47794. SSF47794. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR02239. recomb_RAD51. 1 hit.
PROSITEiPS50162. RECA_2. 1 hit.
PS50163. RECA_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequences of nuclear tRNA(Cys) genes from Nicotiana and Arabidopsis and expression in HeLa cell extract."
    Urban C., Smith K.N., Beier H.
    Plant Mol. Biol. 32:549-552(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Landsberg erecta.
  2. "Isolation and characterisation of the RAD51 and DMC1 homologs from Arabidopsis thaliana."
    Doutriaux M.P., Couteau F., Bergounioux C., White C.
    Mol. Gen. Genet. 257:283-291(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.
    Strain: cv. Columbia.
  3. "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
    Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.
    , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
    Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Elevated UV-B radiation reduces genome stability in plants."
    Ries G., Heller W., Puchta H., Sandermann H., Seidlitz H.K., Hohn B.
    Nature 406:98-101(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  6. "Molecular cloning and characterization of RAD51-like genes from Arabidopsis thaliana."
    Osakabe K., Yoshioka T., Ichikawa H., Toki S.
    Plant Mol. Biol. 50:71-81(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION, SUBUNIT, TISSUE SPECIFICITY, INTERACTION WITH XRCC3.
    Strain: cv. Columbia.
    Tissue: Flower bud.
  7. "AtATM is essential for meiosis and the somatic response to DNA damage in plants."
    Garcia V., Bruchet H., Camescasse D., Granier F., Bouchez D., Tissier A.
    Plant Cell 15:119-132(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  8. "The Arabidopsis AtRAD51 gene is dispensable for vegetative development but required for meiosis."
    Li W., Chen C., Markmann-Mulisch U., Timofejeva L., Schmelzer E., Ma H., Reiss B.
    Proc. Natl. Acad. Sci. U.S.A. 101:10596-10601(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.
  9. "Isolation and characterization of the RAD54 gene from Arabidopsis thaliana."
    Osakabe K., Abe K., Yoshioka T., Osakabe Y., Todoriki S., Ichikawa H., Hohn B., Toki S.
    Plant J. 48:827-842(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RAD54/CHR25, TISSUE SPECIFICITY.
    Strain: cv. Columbia.
    Tissue: Flower bud.
  10. "Interaction between Arabidopsis Brca2 and its partners Rad51, Dmc1, and Dss1."
    Dray E., Siaud N., Dubois E., Doutriaux M.P.
    Plant Physiol. 140:1059-1069(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH BRCA2A AND BRCA2B.

Entry informationi

Entry nameiRAD51_ARATH
AccessioniPrimary (citable) accession number: P94102
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 23, 2004
Last sequence update: May 1, 1997
Last modified: May 11, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.