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Protein

Probable phosphopantothenoylcysteine decarboxylase

Gene

HAL3B

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in plant growth and salt and osmotic tolerance. Catalyzes the decarboxylation of 4'-phosphopantothenoylcysteine to 4'-phosphopantetheine, a key step in coenzyme A biosynthesis. The enzyme is also able to decarboxylate pantothenoylcysteine to pantothenoylcysteamine.1 Publication

Catalytic activityi

N-((R)-4'-phosphopantothenoyl)-L-cysteine = pantotheine 4'-phosphate + CO2.

Cofactori

FMNBy similarityNote: Binds 1 FMN per subunit.By similarity

Pathwayi: coenzyme A biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes CoA from (R)-pantothenate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Pantothenate kinase 2 (PANK2), Pantothenate kinase 1 (PANK1), Pantothenate kinase 2 (AXX17_At4g36860), Pantothenate kinase 2 (PANK2)
  2. Phosphopantothenate--cysteine ligase 2 (PPCS2), Phosphopantothenate--cysteine ligase 1 (PPCS1)
  3. Probable phosphopantothenoylcysteine decarboxylase (HAL3B), Phosphopantothenoylcysteine decarboxylase (HAL3A)
  4. Phosphopantetheine adenylyltransferase (COAD)
  5. Dephospho-CoA kinase (COAE)
This subpathway is part of the pathway coenzyme A biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CoA from (R)-pantothenate, the pathway coenzyme A biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei132FMN; via carbonyl oxygenBy similarity1
Binding sitei134Substrate; via amide nitrogenBy similarity1
Active sitei167Proton donorBy similarity1
Binding sitei175Substrate; via amide nitrogenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi20 – 22FMNBy similarity3
Nucleotide bindingi45 – 47FMNBy similarity3
Nucleotide bindingi98 – 101FMNBy similarity4

GO - Molecular functioni

  • FMN binding Source: TAIR
  • phosphopantothenoylcysteine decarboxylase activity Source: UniProtKB-EC

GO - Biological processi

  • coenzyme A biosynthetic process Source: TAIR
  • hyperosmotic salinity response Source: TAIR
  • regulation of growth Source: UniProtKB-KW

Keywordsi

Molecular functionDecarboxylase, Lyase
Biological processCoenzyme A biosynthesis, Growth regulation, Stress response
LigandFlavoprotein, FMN

Enzyme and pathway databases

BioCyciARA:AT1G48605-MONOMER.
ReactomeiR-ATH-196783. Coenzyme A biosynthesis.
UniPathwayiUPA00241; UER00354.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable phosphopantothenoylcysteine decarboxylase (EC:4.1.1.36)
Alternative name(s):
AtCoaC2
Halotolerance protein Hal3b
Short name:
AtHal3b
Gene namesi
Name:HAL3B
Synonyms:COAC2
Ordered Locus Names:At1g48605
ORF Names:T1N15.24, T1N15.28, T1N15_21
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G48605.
TAIRilocus:1006230763. AT1G48605.

Pathology & Biotechi

Disruption phenotypei

No visible phenotype under normal growth conditions, but homozygous double mutants hal3a-1 and hal3b are embryonic lethal.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001820331 – 201Probable phosphopantothenoylcysteine decarboxylaseAdd BLAST201

Proteomic databases

PaxDbiP94063.

Expressioni

Tissue specificityi

Expressed in roots, shoots, leaves, flowers, developing siliques and seeds.1 Publication

Inductioni

By salt stress.1 Publication

Interactioni

Subunit structurei

Homotrimer.By similarity

Protein-protein interaction databases

STRINGi3702.AT1G48605.1.

Structurei

3D structure databases

ProteinModelPortaliP94063.
SMRiP94063.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni164 – 166Substrate bindingBy similarity3

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0672. Eukaryota.
COG0452. LUCA.
HOGENOMiHOG000187629.
InParanoidiP94063.
KOiK01598.
OMAiCKSHTFG.
OrthoDBiEOG09360KXU.
PhylomeDBiP94063.

Family and domain databases

Gene3Di3.40.50.1950. 1 hit.
InterProiView protein in InterPro
IPR036551. Flavin_trans-like.
IPR003382. Flavoprotein.
PfamiView protein in Pfam
PF02441. Flavoprotein. 1 hit.
SUPFAMiSSF52507. SSF52507. 1 hit.

Sequencei

Sequence statusi: Complete.

P94063-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNMEVDTVTR KPRILLAASG SVASIKFSNL CHCFSEWAEV KAVASKSSLN
60 70 80 90 100
FVDKPSLPQN VTLYTDEDEW SSWNKIGDPV LHIELRRWAD VMIIAPLSAN
110 120 130 140 150
TLAKIAGGLC DNLLTCIVRA WDYSKPLFVA PAMNTLMWNN PFTERHLVLL
160 170 180 190 200
DELGITLIPP IKKKLACGDY GNGAMAEPSL IYSTVRLFWE SQARKQRDGT

S
Length:201
Mass (Da):22,415
Last modified:July 1, 1997 - v2
Checksum:i7962655E408FA64C
GO

Sequence cautioni

The sequence AAF79709 differs from that shown. Reason: Erroneous gene model prediction. The predicted gene At1g48610 has been split into 2 genes: At1g48605 and At1g48610.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U80192 Genomic DNA. Translation: AAB53106.1.
AC020889 Genomic DNA. Translation: AAF79709.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE32325.1.
RefSeqiNP_973994.1. NM_202265.3.
UniGeneiAt.47622.

Genome annotation databases

EnsemblPlantsiAT1G48605.1; AT1G48605.1; AT1G48605.
GeneIDi2745818.
GrameneiAT1G48605.1; AT1G48605.1; AT1G48605.
KEGGiath:AT1G48605.

Similar proteinsi

Entry informationi

Entry nameiHAL3B_ARATH
AccessioniPrimary (citable) accession number: P94063
Secondary accession number(s): Q9LP62
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: July 1, 1997
Last modified: October 25, 2017
This is version 118 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Caution

Was previously called At1g48610.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families