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P93846

- CP51_SORBI

UniProt

P93846 - CP51_SORBI

Protein

Obtusifoliol 14-alpha demethylase

Gene

CYP51

Organism
Sorghum bicolor (Sorghum) (Sorghum vulgare)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 91 (01 Oct 2014)
      Sequence version 1 (01 May 1997)
      Previous versions | rss
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    Functioni

    Catalyzes the 14-alpha demethylation of obtusifoliol to 4 alpha-methyl-5 alpha-ergosta-8,14,24(28)-trien-3 beta-ol.

    Catalytic activityi

    A 14-alpha-methylsteroid + 3 O2 + 3 NADPH = a Delta(14)-steroid + formate + 3 NADP+ + 4 H2O.

    Cofactori

    Heme group.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi434 – 4341Iron (heme axial ligand)By similarity

    GO - Molecular functioni

    1. heme binding Source: InterPro
    2. iron ion binding Source: InterPro
    3. methyltransferase activity Source: UniProtKB-KW
    4. sterol 14-demethylase activity Source: UniProtKB-EC

    GO - Biological processi

    1. sterol biosynthetic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Methyltransferase, Monooxygenase, Oxidoreductase, Transferase

    Keywords - Biological processi

    Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

    Keywords - Ligandi

    Heme, Iron, Metal-binding, NADP

    Enzyme and pathway databases

    UniPathwayiUPA00770; UER00754.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Obtusifoliol 14-alpha demethylase (EC:1.14.13.70)
    Alternative name(s):
    CYPLI
    Cytochrome P450 51
    Cytochrome P450-LIA1
    Gene namesi
    Name:CYP51
    OrganismiSorghum bicolor (Sorghum) (Sorghum vulgare)
    Taxonomic identifieri4558 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogoneaeSorghum

    Organism-specific databases

    GrameneiP93846.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum Source: EnsemblPlants/Gramene
    2. Golgi apparatus Source: EnsemblPlants/Gramene
    3. integral component of membrane Source: UniProtKB-KW
    4. plasma membrane Source: EnsemblPlants/Gramene

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 492492Obtusifoliol 14-alpha demethylasePRO_0000052013Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi4558.Sb08g002250.1.

    Structurei

    3D structure databases

    ProteinModelPortaliP93846.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei12 – 3221HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG2124.
    HOGENOMiHOG000042780.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002403. Cyt_P450_E_grp-IV.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00465. EP450IV.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P93846-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDLADIPQQQ RLMAGLALVV ATVIFLKLLL SFRSGGGKKR LPPTIPGAPV    50
    VGGLVKFMRG PIPMIREQYA ALGSVFTVPI ITRRITFLIG PEVSAHFFKG 100
    NEAEMSQQEV YRFNVPTFGP GVVFDVDYSV RQEQFRFFTE ALRANKLRSY 150
    VDQMVAEAEE YFSKWGESGT VDLKYELEHL IILTASRCLL GREVREKLFD 200
    DVSALFHDLD NGIQPISVLF PYLPIPAHKR RDKARARLAE IFATIIKSRK 250
    ASGQSEEDML QCFIDSKYKN GRPTTEGEVT GLLIAALFAG QHTSSITSTW 300
    TGAYMLRFKQ YFAEAVEEQK DVMKRHGDKI DHDILAEMDV LYRCIKEALR 350
    LHPPLIMLLR QSHSDFTVTT KEGKEYDIPK GHIVATSPSF ANRLPHIYKN 400
    PDSYDPDRFG PGREEDKAAG AFSYISFGGG RHGCLGEPFA YLQIKAIWTH 450
    LLRNFEFELV SPFPENDWNA MVVGIKGEVM VNYKRRKLVV DN 492
    Length:492
    Mass (Da):55,653
    Last modified:May 1, 1997 - v1
    Checksum:i0C86160DFD812ECE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U74319 mRNA. Translation: AAC49659.1.
    PIRiT14820.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U74319 mRNA. Translation: AAC49659.1 .
    PIRi T14820.

    3D structure databases

    ProteinModelPortali P93846.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 4558.Sb08g002250.1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Organism-specific databases

    Gramenei P93846.

    Phylogenomic databases

    eggNOGi COG2124.
    HOGENOMi HOG000042780.

    Enzyme and pathway databases

    UniPathwayi UPA00770 ; UER00754 .

    Family and domain databases

    Gene3Di 1.10.630.10. 1 hit.
    InterProi IPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002403. Cyt_P450_E_grp-IV.
    [Graphical view ]
    Pfami PF00067. p450. 1 hit.
    [Graphical view ]
    PRINTSi PR00465. EP450IV.
    PR00385. P450.
    SUPFAMi SSF48264. SSF48264. 1 hit.
    PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and expression in Escherichia coli of the obtusifoliol 14 alpha-demethylase of Sorghum bicolor (L.) Moench, a cytochrome P450 orthologous to the sterol 14 alpha-demethylases (CYP51) from fungi and mammals."
      Bak S., Kahn R.A., Olsen C.E., Halkier B.A.
      Plant J. 11:191-201(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
      Strain: cv. SS1000.
      Tissue: Etiolated seedling.

    Entry informationi

    Entry nameiCP51_SORBI
    AccessioniPrimary (citable) accession number: P93846
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: May 1, 1997
    Last modified: October 1, 2014
    This is version 91 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3