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P93846 (CP51_SORBI) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Obtusifoliol 14-alpha demethylase

EC=1.14.13.70
Alternative name(s):
CYPLI
Cytochrome P450 51
Cytochrome P450-LIA1
Gene names
Name:CYP51
OrganismSorghum bicolor (Sorghum) (Sorghum vulgare)
Taxonomic identifier4558 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogoneaeSorghum

Protein attributes

Sequence length492 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the 14-alpha demethylation of obtusifoliol to 4 alpha-methyl-5 alpha-ergosta-8,14,24(28)-trien-3 beta-ol.

Catalytic activity

A 14-alpha-methylsteroid + 3 O2 + 3 NADPH = a Delta(14)-steroid + formate + 3 NADP+ + 4 H2O.

Cofactor

Heme group.

Pathway

Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 1/6.

Subcellular location

Membrane; Single-pass membrane protein Potential.

Sequence similarities

Belongs to the cytochrome P450 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 492492Obtusifoliol 14-alpha demethylase
PRO_0000052013

Regions

Transmembrane12 – 3221Helical; Potential

Sites

Metal binding4341Iron (heme axial ligand) By similarity

Sequences

Sequence LengthMass (Da)Tools
P93846 [UniParc].

Last modified May 1, 1997. Version 1.
Checksum: 0C86160DFD812ECE

FASTA49255,653
        10         20         30         40         50         60 
MDLADIPQQQ RLMAGLALVV ATVIFLKLLL SFRSGGGKKR LPPTIPGAPV VGGLVKFMRG 

        70         80         90        100        110        120 
PIPMIREQYA ALGSVFTVPI ITRRITFLIG PEVSAHFFKG NEAEMSQQEV YRFNVPTFGP 

       130        140        150        160        170        180 
GVVFDVDYSV RQEQFRFFTE ALRANKLRSY VDQMVAEAEE YFSKWGESGT VDLKYELEHL 

       190        200        210        220        230        240 
IILTASRCLL GREVREKLFD DVSALFHDLD NGIQPISVLF PYLPIPAHKR RDKARARLAE 

       250        260        270        280        290        300 
IFATIIKSRK ASGQSEEDML QCFIDSKYKN GRPTTEGEVT GLLIAALFAG QHTSSITSTW 

       310        320        330        340        350        360 
TGAYMLRFKQ YFAEAVEEQK DVMKRHGDKI DHDILAEMDV LYRCIKEALR LHPPLIMLLR 

       370        380        390        400        410        420 
QSHSDFTVTT KEGKEYDIPK GHIVATSPSF ANRLPHIYKN PDSYDPDRFG PGREEDKAAG 

       430        440        450        460        470        480 
AFSYISFGGG RHGCLGEPFA YLQIKAIWTH LLRNFEFELV SPFPENDWNA MVVGIKGEVM 

       490 
VNYKRRKLVV DN 

« Hide

References

[1]"Cloning and expression in Escherichia coli of the obtusifoliol 14 alpha-demethylase of Sorghum bicolor (L.) Moench, a cytochrome P450 orthologous to the sterol 14 alpha-demethylases (CYP51) from fungi and mammals."
Bak S., Kahn R.A., Olsen C.E., Halkier B.A.
Plant J. 11:191-201(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
Strain: cv. SS1000.
Tissue: Etiolated seedling.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U74319 mRNA. Translation: AAC49659.1.
PIRT14820.

3D structure databases

ProteinModelPortalP93846.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING4558.Sb08g002250.1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

GrameneP93846.

Phylogenomic databases

eggNOGCOG2124.
HOGENOMHOG000042780.

Enzyme and pathway databases

UniPathwayUPA00770; UER00754.

Family and domain databases

Gene3D1.10.630.10. 1 hit.
InterProIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002403. Cyt_P450_E_grp-IV.
[Graphical view]
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00465. EP450IV.
PR00385. P450.
SUPFAMSSF48264. SSF48264. 1 hit.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCP51_SORBI
AccessionPrimary (citable) accession number: P93846
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: May 1, 1997
Last modified: July 9, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways