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P93846

- CP51_SORBI

UniProt

P93846 - CP51_SORBI

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Protein
Obtusifoliol 14-alpha demethylase
Gene
CYP51
Organism
Sorghum bicolor (Sorghum) (Sorghum vulgare)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the 14-alpha demethylation of obtusifoliol to 4 alpha-methyl-5 alpha-ergosta-8,14,24(28)-trien-3 beta-ol.

Catalytic activityi

A 14-alpha-methylsteroid + 3 O2 + 3 NADPH = a Delta(14)-steroid + formate + 3 NADP+ + 4 H2O.

Cofactori

Heme group.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi434 – 4341Iron (heme axial ligand) By similarity

GO - Molecular functioni

  1. heme binding Source: InterPro
  2. iron ion binding Source: InterPro
  3. methyltransferase activity Source: UniProtKB-KW
  4. sterol 14-demethylase activity Source: UniProtKB-EC

GO - Biological processi

  1. sterol biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Monooxygenase, Oxidoreductase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

Keywords - Ligandi

Heme, Iron, Metal-binding, NADP

Enzyme and pathway databases

UniPathwayiUPA00770; UER00754.

Names & Taxonomyi

Protein namesi
Recommended name:
Obtusifoliol 14-alpha demethylase (EC:1.14.13.70)
Alternative name(s):
CYPLI
Cytochrome P450 51
Cytochrome P450-LIA1
Gene namesi
Name:CYP51
OrganismiSorghum bicolor (Sorghum) (Sorghum vulgare)
Taxonomic identifieri4558 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogoneaeSorghum

Organism-specific databases

GrameneiP93846.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei12 – 3221Helical; Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. Golgi apparatus Source: EnsemblPlants/Gramene
  2. endoplasmic reticulum Source: EnsemblPlants/Gramene
  3. integral component of membrane Source: UniProtKB-KW
  4. plasma membrane Source: EnsemblPlants/Gramene
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 492492Obtusifoliol 14-alpha demethylase
PRO_0000052013Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi4558.Sb08g002250.1.

Structurei

3D structure databases

ProteinModelPortaliP93846.

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG2124.
HOGENOMiHOG000042780.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002403. Cyt_P450_E_grp-IV.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00465. EP450IV.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P93846-1 [UniParc]FASTAAdd to Basket

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MDLADIPQQQ RLMAGLALVV ATVIFLKLLL SFRSGGGKKR LPPTIPGAPV    50
VGGLVKFMRG PIPMIREQYA ALGSVFTVPI ITRRITFLIG PEVSAHFFKG 100
NEAEMSQQEV YRFNVPTFGP GVVFDVDYSV RQEQFRFFTE ALRANKLRSY 150
VDQMVAEAEE YFSKWGESGT VDLKYELEHL IILTASRCLL GREVREKLFD 200
DVSALFHDLD NGIQPISVLF PYLPIPAHKR RDKARARLAE IFATIIKSRK 250
ASGQSEEDML QCFIDSKYKN GRPTTEGEVT GLLIAALFAG QHTSSITSTW 300
TGAYMLRFKQ YFAEAVEEQK DVMKRHGDKI DHDILAEMDV LYRCIKEALR 350
LHPPLIMLLR QSHSDFTVTT KEGKEYDIPK GHIVATSPSF ANRLPHIYKN 400
PDSYDPDRFG PGREEDKAAG AFSYISFGGG RHGCLGEPFA YLQIKAIWTH 450
LLRNFEFELV SPFPENDWNA MVVGIKGEVM VNYKRRKLVV DN 492
Length:492
Mass (Da):55,653
Last modified:May 1, 1997 - v1
Checksum:i0C86160DFD812ECE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U74319 mRNA. Translation: AAC49659.1.
PIRiT14820.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U74319 mRNA. Translation: AAC49659.1 .
PIRi T14820.

3D structure databases

ProteinModelPortali P93846.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 4558.Sb08g002250.1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Organism-specific databases

Gramenei P93846.

Phylogenomic databases

eggNOGi COG2124.
HOGENOMi HOG000042780.

Enzyme and pathway databases

UniPathwayi UPA00770 ; UER00754 .

Family and domain databases

Gene3Di 1.10.630.10. 1 hit.
InterProi IPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002403. Cyt_P450_E_grp-IV.
[Graphical view ]
Pfami PF00067. p450. 1 hit.
[Graphical view ]
PRINTSi PR00465. EP450IV.
PR00385. P450.
SUPFAMi SSF48264. SSF48264. 1 hit.
PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and expression in Escherichia coli of the obtusifoliol 14 alpha-demethylase of Sorghum bicolor (L.) Moench, a cytochrome P450 orthologous to the sterol 14 alpha-demethylases (CYP51) from fungi and mammals."
    Bak S., Kahn R.A., Olsen C.E., Halkier B.A.
    Plant J. 11:191-201(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Strain: cv. SS1000.
    Tissue: Etiolated seedling.

Entry informationi

Entry nameiCP51_SORBI
AccessioniPrimary (citable) accession number: P93846
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: May 1, 1997
Last modified: July 9, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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