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Protein

Obtusifoliol 14-alpha demethylase

Gene

CYP51

Organism
Sorghum bicolor (Sorghum) (Sorghum vulgare)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the 14-alpha demethylation of obtusifoliol to 4 alpha-methyl-5 alpha-ergosta-8,14,24(28)-trien-3 beta-ol.

Catalytic activityi

A 14-alpha-methylsteroid + 3 O2 + 3 NADPH = a Delta(14)-steroid + formate + 3 NADP+ + 4 H2O.

Cofactori

Pathwayi: zymosterol biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes zymosterol from lanosterol.
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Obtusifoliol 14-alpha demethylase (CYP51)
  2. no protein annotated in this organism
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. no protein annotated in this organism
  6. no protein annotated in this organism
This subpathway is part of the pathway zymosterol biosynthesis, which is itself part of Steroid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes zymosterol from lanosterol, the pathway zymosterol biosynthesis and in Steroid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi434Iron (heme axial ligand)By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Monooxygenase, Oxidoreductase, Transferase
Biological processLipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism
LigandHeme, Iron, Metal-binding, NADP

Enzyme and pathway databases

UniPathwayiUPA00770; UER00754

Names & Taxonomyi

Protein namesi
Recommended name:
Obtusifoliol 14-alpha demethylase (EC:1.14.13.70)
Alternative name(s):
CYPLI
Cytochrome P450 51
Cytochrome P450-LIA1
Gene namesi
Name:CYP51
OrganismiSorghum bicolor (Sorghum) (Sorghum vulgare)
Taxonomic identifieri4558 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogonodaeAndropogoneaeSorghinaeSorghum

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei12 – 32HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2366482

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000520131 – 492Obtusifoliol 14-alpha demethylaseAdd BLAST492

Expressioni

Gene expression databases

ExpressionAtlasiP93846 baseline

Structurei

3D structure databases

ProteinModelPortaliP93846
SMRiP93846
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0684 Eukaryota
COG2124 LUCA
HOGENOMiHOG000042780

Family and domain databases

Gene3Di1.10.630.10, 1 hit
InterProiView protein in InterPro
IPR001128 Cyt_P450
IPR017972 Cyt_P450_CS
IPR002403 Cyt_P450_E_grp-IV
IPR036396 Cyt_P450_sf
PfamiView protein in Pfam
PF00067 p450, 1 hit
PRINTSiPR00465 EP450IV
PR00385 P450
SUPFAMiSSF48264 SSF48264, 1 hit
PROSITEiView protein in PROSITE
PS00086 CYTOCHROME_P450, 1 hit

Sequencei

Sequence statusi: Complete.

P93846-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDLADIPQQQ RLMAGLALVV ATVIFLKLLL SFRSGGGKKR LPPTIPGAPV
60 70 80 90 100
VGGLVKFMRG PIPMIREQYA ALGSVFTVPI ITRRITFLIG PEVSAHFFKG
110 120 130 140 150
NEAEMSQQEV YRFNVPTFGP GVVFDVDYSV RQEQFRFFTE ALRANKLRSY
160 170 180 190 200
VDQMVAEAEE YFSKWGESGT VDLKYELEHL IILTASRCLL GREVREKLFD
210 220 230 240 250
DVSALFHDLD NGIQPISVLF PYLPIPAHKR RDKARARLAE IFATIIKSRK
260 270 280 290 300
ASGQSEEDML QCFIDSKYKN GRPTTEGEVT GLLIAALFAG QHTSSITSTW
310 320 330 340 350
TGAYMLRFKQ YFAEAVEEQK DVMKRHGDKI DHDILAEMDV LYRCIKEALR
360 370 380 390 400
LHPPLIMLLR QSHSDFTVTT KEGKEYDIPK GHIVATSPSF ANRLPHIYKN
410 420 430 440 450
PDSYDPDRFG PGREEDKAAG AFSYISFGGG RHGCLGEPFA YLQIKAIWTH
460 470 480 490
LLRNFEFELV SPFPENDWNA MVVGIKGEVM VNYKRRKLVV DN
Length:492
Mass (Da):55,653
Last modified:May 1, 1997 - v1
Checksum:i0C86160DFD812ECE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U74319 mRNA Translation: AAC49659.1
PIRiT14820
UniGeneiSbi.13512

Similar proteinsi

Entry informationi

Entry nameiCP51_SORBI
AccessioniPrimary (citable) accession number: P93846
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: May 1, 1997
Last modified: May 23, 2018
This is version 103 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

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