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P93846

- CP51_SORBI

UniProt

P93846 - CP51_SORBI

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Protein

Obtusifoliol 14-alpha demethylase

Gene

CYP51

Organism
Sorghum bicolor (Sorghum) (Sorghum vulgare)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the 14-alpha demethylation of obtusifoliol to 4 alpha-methyl-5 alpha-ergosta-8,14,24(28)-trien-3 beta-ol.

Catalytic activityi

A 14-alpha-methylsteroid + 3 O2 + 3 NADPH = a Delta(14)-steroid + formate + 3 NADP+ + 4 H2O.

Cofactori

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi434 – 4341Iron (heme axial ligand)By similarity

GO - Molecular functioni

  1. heme binding Source: InterPro
  2. iron ion binding Source: InterPro
  3. methyltransferase activity Source: UniProtKB-KW
  4. sterol 14-demethylase activity Source: UniProtKB-EC

GO - Biological processi

  1. sterol biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Monooxygenase, Oxidoreductase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

Keywords - Ligandi

Heme, Iron, Metal-binding, NADP

Enzyme and pathway databases

UniPathwayiUPA00770; UER00754.

Names & Taxonomyi

Protein namesi
Recommended name:
Obtusifoliol 14-alpha demethylase (EC:1.14.13.70)
Alternative name(s):
CYPLI
Cytochrome P450 51
Cytochrome P450-LIA1
Gene namesi
Name:CYP51
OrganismiSorghum bicolor (Sorghum) (Sorghum vulgare)
Taxonomic identifieri4558 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogoneaeSorghum

Organism-specific databases

GrameneiP93846.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei12 – 3221HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 492492Obtusifoliol 14-alpha demethylasePRO_0000052013Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi4558.Sb08g002250.1.

Structurei

3D structure databases

ProteinModelPortaliP93846.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG2124.
HOGENOMiHOG000042780.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002403. Cyt_P450_E_grp-IV.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00465. EP450IV.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P93846-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDLADIPQQQ RLMAGLALVV ATVIFLKLLL SFRSGGGKKR LPPTIPGAPV
60 70 80 90 100
VGGLVKFMRG PIPMIREQYA ALGSVFTVPI ITRRITFLIG PEVSAHFFKG
110 120 130 140 150
NEAEMSQQEV YRFNVPTFGP GVVFDVDYSV RQEQFRFFTE ALRANKLRSY
160 170 180 190 200
VDQMVAEAEE YFSKWGESGT VDLKYELEHL IILTASRCLL GREVREKLFD
210 220 230 240 250
DVSALFHDLD NGIQPISVLF PYLPIPAHKR RDKARARLAE IFATIIKSRK
260 270 280 290 300
ASGQSEEDML QCFIDSKYKN GRPTTEGEVT GLLIAALFAG QHTSSITSTW
310 320 330 340 350
TGAYMLRFKQ YFAEAVEEQK DVMKRHGDKI DHDILAEMDV LYRCIKEALR
360 370 380 390 400
LHPPLIMLLR QSHSDFTVTT KEGKEYDIPK GHIVATSPSF ANRLPHIYKN
410 420 430 440 450
PDSYDPDRFG PGREEDKAAG AFSYISFGGG RHGCLGEPFA YLQIKAIWTH
460 470 480 490
LLRNFEFELV SPFPENDWNA MVVGIKGEVM VNYKRRKLVV DN
Length:492
Mass (Da):55,653
Last modified:May 1, 1997 - v1
Checksum:i0C86160DFD812ECE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U74319 mRNA. Translation: AAC49659.1.
PIRiT14820.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U74319 mRNA. Translation: AAC49659.1 .
PIRi T14820.

3D structure databases

ProteinModelPortali P93846.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 4558.Sb08g002250.1.

Chemistry

ChEMBLi CHEMBL2366482.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Organism-specific databases

Gramenei P93846.

Phylogenomic databases

eggNOGi COG2124.
HOGENOMi HOG000042780.

Enzyme and pathway databases

UniPathwayi UPA00770 ; UER00754 .

Family and domain databases

Gene3Di 1.10.630.10. 1 hit.
InterProi IPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002403. Cyt_P450_E_grp-IV.
[Graphical view ]
Pfami PF00067. p450. 1 hit.
[Graphical view ]
PRINTSi PR00465. EP450IV.
PR00385. P450.
SUPFAMi SSF48264. SSF48264. 1 hit.
PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and expression in Escherichia coli of the obtusifoliol 14 alpha-demethylase of Sorghum bicolor (L.) Moench, a cytochrome P450 orthologous to the sterol 14 alpha-demethylases (CYP51) from fungi and mammals."
    Bak S., Kahn R.A., Olsen C.E., Halkier B.A.
    Plant J. 11:191-201(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Strain: cv. SS1000.
    Tissue: Etiolated seedling.

Entry informationi

Entry nameiCP51_SORBI
AccessioniPrimary (citable) accession number: P93846
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: May 1, 1997
Last modified: November 26, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3