Reviewed, UniProtKB/Swiss-Prot P93846 (CP51_SORBI)

Names and origin

Protein names

Recommended name:
    Obtusifoliol 14-alpha demethylase
    EC=1.14.13.70
Alternative name(s):
    Cytochrome P450 51
    CYPLI
    Cytochrome P450-LIA1

Gene names

Name:

CYP51

Organism

Sorghum bicolor (Sorghum) (Sorghum vulgare)

Taxonomic identifier

4558 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › PACCAD clade › Panicoideae › Andropogoneae › Sorghum

Protein attributes

Sequence length	492 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the 14-alpha demethylation of obtusifoliol to 4 alpha-methyl-5 alpha-ergosta-8,14,24(28)-trien-3 beta-ol.
Catalytic activity	Obtusifoliol + 3 O2 + 3 NADPH = 4-alpha-methyl-5-alpha-ergosta-8,14,24(28)-trien-3-beta-ol + formate + 3 NADP+ + 4 H2O.
Cofactor	Heme group.
Pathway	Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 1/6.
Subcellular location	Membrane Potential. Membrane; Single-pass membrane protein.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Biological process	Lipid synthesis Steroid biosynthesis Sterol biosynthesis
Cellular component	Membrane
Domain	Transmembrane
Ligand	Heme Iron Metal-binding NADP
Molecular function	Methyltransferase Monooxygenase Oxidoreductase Transferase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW sterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW sterol 14-demethylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 492	492	Obtusifoliol 14-alpha demethylase		PRO_0000052013
Regions
	Transmembrane	12 – 32	21	Potential
Sites
	Metal binding	434	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P93846-1 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 0C86160DFD812ECE	FASTA	492	55,653
	10 20 30 40 50 60 MDLADIPQQQ RLMAGLALVV ATVIFLKLLL SFRSGGGKKR LPPTIPGAPV VGGLVKFMRG 70 80 90 100 110 120 PIPMIREQYA ALGSVFTVPI ITRRITFLIG PEVSAHFFKG NEAEMSQQEV YRFNVPTFGP 130 140 150 160 170 180 GVVFDVDYSV RQEQFRFFTE ALRANKLRSY VDQMVAEAEE YFSKWGESGT VDLKYELEHL 190 200 210 220 230 240 IILTASRCLL GREVREKLFD DVSALFHDLD NGIQPISVLF PYLPIPAHKR RDKARARLAE 250 260 270 280 290 300 IFATIIKSRK ASGQSEEDML QCFIDSKYKN GRPTTEGEVT GLLIAALFAG QHTSSITSTW 310 320 330 340 350 360 TGAYMLRFKQ YFAEAVEEQK DVMKRHGDKI DHDILAEMDV LYRCIKEALR LHPPLIMLLR 370 380 390 400 410 420 QSHSDFTVTT KEGKEYDIPK GHIVATSPSF ANRLPHIYKN PDSYDPDRFG PGREEDKAAG 430 440 450 460 470 480 AFSYISFGGG RHGCLGEPFA YLQIKAIWTH LLRNFEFELV SPFPENDWNA MVVGIKGEVM 490 VNYKRRKLVV DN

References

[1]

"Cloning and expression in Escherichia coli of the obtusifoliol 14 alpha-demethylase of Sorghum bicolor (L.) Moench, a cytochrome P450 orthologous to the sterol 14 alpha-demethylases (CYP51) from fungi and mammals." Bak S., Kahn R.A., Olsen C.E., Halkier B.A. Plant J. 11:191-201(1997) [PubMed: 9076987] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Strain: cv. SS1000. Tissue: Etiolated seedling.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U74319 mRNA. Translation: AAC49659.1.
PIR	T14820.
3D structure databases
SMR	P93846. Positions 39-487.
ModBase	Search...
Organism-specific databases
Gramene	P93846.
Enzyme and pathway databases
BRENDA	1.14.13.70. 1396.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002403. Cyt_P450_E_grp-IV. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00465. EP450IV. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CP51_SORBI

Accession

Primary (citable) accession number: P93846

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	May 1, 1997
Last modified:	January 19, 2010
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)