Skip Header

 
Contribute Send feedback
Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot P93844 (PLDA2_ORYSJ)

Last modified June 16, 2009. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Hide | Top

Names and origin

Protein namesRecommended name:
    Phospholipase D alpha 2
      Short name=PLD alpha 2
    EC=3.1.4.4
Alternative name(s):
    Choline phosphatase 2
    Phosphatidylcholine-hydrolyzing phospholipase D 2
Gene names
Name: PLD2
Ordered Locus Names: Os06g0604400, LOC_Os06g40190
ORF Names: P0481H08.5
OrganismOryza sativa subsp. japonica (Rice)
Taxonomic identifier39947 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Hide | Top

Protein attributes

Sequence length817 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at transcript level.

Hide | Top

General annotation (Comments)

Function

Hydrolyzes glycerol-phospholipids at the terminal phosphodiesteric bond. Plays an important role in various cellular processes.

Catalytic activity

A phosphatidylcholine + H2O = choline + a phosphatidate.

Cofactor

Calcium By similarity.

Domain

C2 domain is a calcium-binding fold, and the binding promotes the protein association with membranes. A lower affinity toward calcium can be anticipated for PLD alpha due to the absence of two potential calcium ligands.

Sequence similarities

Belongs to the phospholipase D family. C2-PLD subfamily.

Contains 1 C2 domain.

Contains 2 PLD phosphodiesterase domains.

Hide | Top

Ontologies

Keywords
   Biological processLipid degradation
   DomainRepeat
   LigandCalcium
   Molecular functionHydrolase
Gene Ontology (GO)
   Biological processlipid catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

phosphatidylcholine metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular componentmembrane

Inferred from electronic annotation. Source: InterPro

   Molecular functionNAPE-specific phospholipase D activity

Inferred from electronic annotation. Source: EC

calcium ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

phospholipase D activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Hide | Top

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 817817Phospholipase D alpha 2
PRO_0000218821

Regions

Domain1 – 114114C2
Domain333 – 37240PLD phosphodiesterase 1
Domain663 – 69028PLD phosphodiesterase 2

Sites

Active site3381 Potential
Active site3401 Potential
Active site3451 Potential
Active site6681 Potential
Active site6701 Potential
Active site6751 Potential

Experimental info

Sequence conflict1111G → D in BAA19466. Ref.1
Sequence conflict1221S → C in BAA19466. Ref.1
Sequence conflict3651G → R in BAA19466. Ref.1
Sequence conflict3871A → G in BAA19466. Ref.1
Sequence conflict3911D → Y in BAA19466. Ref.1
Sequence conflict3951P → A in BAA19466. Ref.1
Sequence conflict4801G → WA in BAA19466. Ref.1
Sequence conflict5201A → R in BAA19466. Ref.1
Sequence conflict5501A → P in BAA19466. Ref.1
Sequence conflict6901S → P in BAA19466. Ref.1
Sequence conflict694 – 6952GR → EG in BAA19466. Ref.1
Sequence conflict7291Y → F Ref.3

Hide | Top

Sequences

Sequence LengthMass (Da)Tools
P93844-1 [UniParc].

Last modified June 13, 2006. Version 2.
Checksum: 948AE6A40B32E12F

FASTA81791,319
        10         20         30         40         50         60 
MAHLLLHGTL EATILEADHL SNPTRATGAA PGIFRKFVEG FEDSLGLGKG ATRLYATIDL 

        70         80         90        100        110        120 
GRARVGRTRV VDDEPVNPRW YEVFHIYCAH FAADVVFSVK AAQPIGATLI GRAYLPVREL 

       130        140        150        160        170        180 
LSGEAIERRL DILDAGRRRI SHGPTIHVRL QFRDVAGDRH GWGRGVSGAR YPGVPYTFFS 

       190        200        210        220        230        240 
QRPGCRVTLY QDAHVPDAFA PRIPLAGGGY YRQGRCWEDV FDAISNAKHL IYLTGWSVYT 

       250        260        270        280        290        300 
EITLIRDGTR QRPGGDATLG ELLKRKASEG VRVLLLVWDD RTSVESLGMK WGFMSTHDAE 

       310        320        330        340        350        360 
TADYFRGTDV RCVLCPRNPD AGRSAIMGAQ IAYMITHHQK TVIVDHDMPV PRGGGSRRIV 

       370        380        390        400        410        420 
SFVGGLDLCD GRYDTQFHSL FRTLDTAHHS DFHQPNLDGA AVTKGGPREP WHDIHSKIEG 

       430        440        450        460        470        480 
PAAWDVLYNF EQRWRKQGGD KDLLLDLKAM ADLIIPPSPV MFPDDGEAWS VQLFRSIDGG 

       490        500        510        520        530        540 
ACFGFPSTPE AAARSGLVSG KNNTIDRSIQ DAYIHAIRRA KNFIYIENQY FLGSSFAWKA 

       550        560        570        580        590        600 
DGIRPEDIEA LHLIPREISL KIVNKIEAGE RFAVYVVLPM WPEGPPASGS VQAILDWQRR 

       610        620        630        640        650        660 
TMEMMYYDIA VALEAKRINA DPRDYLTFFC LGNREVKLNG EYEPAGRPLD GTDYAKAQKA 

       670        680        690        700        710        720 
RRFMIYVHSK MMIVDDEYII VGSANINQRS MDGGRDSEIA MGAFQPCHLN TKGLVARGQI 

       730        740        750        760        770        780 
HGFRMSLWYE HLGMLHDNFL NPESLECVQR VNKMADKYWD LYASDELNDD LPGHLLTYPV 

       790        800        810 
RVTKEGTVTE LPGAKFFPDT QAPVIGTKGN LPPFLTT

« Hide

Hide | Top

References

« Hide 'large scale' references
[1]"Characterization of two Distinctive genomic clones for phospholipase D from rice."
Morioka S., Ueki J., Komari T.
Plant Gene Register PGR97-076
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: cv. Koshihikari.
Tissue: Leaf.
[2]"Oryza sativa nipponbare chromosome 6 genomic DNA sequence."
The international rice genome sequencing project (IRGSP) consortium
Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[3]"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
The rice full-length cDNA consortium
Science 301:376-379(2003) [PubMed: 12869764] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Nipponbare.

Hide | Top

Cross-references

Sequence databases

AB001919 Genomic DNA. Translation: BAA19466.1.
AP003629 Genomic DNA. Translation: BAD35531.1.
AK072121 mRNA. No translation available.
PIRT03401.
RefSeqNP_001058017.1.
UniGeneOs.9878

3D structure databases

ModBaseSearch...

Genome annotation databases

GeneID4341469.
KEGGosa:4341469.

Organism-specific databases

GrameneP93844.

Family and domain databases

InterProIPR000008. C2_Ca-dep.
IPR015679. Phospholipase_D.
IPR011402. PLD_pln.
IPR001736. PLipase_D/transphosphatidylase.
[Graphical view]
PANTHERPTHR18896. Phospholipase_D. 1 hit.
PfamPF00168. C2. 1 hit.
PF00614. PLDc. 2 hits.
[Graphical view]
PIRSFPIRSF036470. PLD_plant. 1 hit.
SMARTSM00239. C2. 1 hit.
SM00155. PLDc. 2 hits.
[Graphical view]
PROSITEPS50004. C2. False negative.
PS50035. PLD. 2 hits.
[Graphical view]
ProtoNetSearch...

Hide | Top

Entry information

Entry namePLDA2_ORYSJ
AccessionPrimary (citable) accession number: P93844
Secondary accession number(s): Q69X20
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: June 13, 2006
Last modified: June 16, 2009
This is version 63 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

Hide | Top

Relevant documents

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents