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P93836 (HPPD_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 110. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
4-hydroxyphenylpyruvate dioxygenase

EC=1.13.11.27
Alternative name(s):
4-hydroxyphenylpyruvic acid oxidase
Short name=4HPPD
Short name=HPD
Short name=HPPDase
Gene names
Name:HPD
Synonyms:PDS1
Ordered Locus Names:At1g06570
ORF Names:F12K11.9
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonscore eudicotyledonsrosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length445 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

4-hydroxyphenylpyruvate + O2 = homogentisate + CO2.

Cofactor

Binds 1 iron ion per subunit. Ref.7

Pathway

Amino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 3/6.

Cofactor biosynthesis; prenylquinone biosynthesis.

Subunit structure

Homodimer. Ref.7 Ref.8

Subcellular location

Cytoplasm Ref.3.

Sequence similarities

Belongs to the 4HPPD family.

Sequence caution

The sequence AAF24813.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

The sequence AEE28006.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
   Biological processPhenylalanine catabolism
Tyrosine catabolism
   Cellular componentCytoplasm
   Coding sequence diversityAlternative splicing
   LigandIron
Metal-binding
   Molecular functionDioxygenase
Oxidoreductase
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processL-phenylalanine catabolic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

tyrosine catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_function4-hydroxyphenylpyruvate dioxygenase activity

Inferred from electronic annotation. Source: EC

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

itself2EBI-1251387,EBI-1251387

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: P93836-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 4454454-hydroxyphenylpyruvate dioxygenase
PRO_0000088397

Sites

Metal binding2261Iron
Metal binding3081Iron
Metal binding3941Iron

Secondary structure

..................................................................... 445
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified January 1, 1998. Version 2.
Checksum: FD0442F93556B3F5

FASTA44548,816
        10         20         30         40         50         60 
MGHQNAAVSE NQNHDDGAAS SPGFKLVGFS KFVRKNPKSD KFKVKRFHHI EFWCGDATNV 

        70         80         90        100        110        120 
ARRFSWGLGM RFSAKSDLST GNMVHASYLL TSGDLRFLFT APYSPSLSAG EIKPTTTASI 

       130        140        150        160        170        180 
PSFDHGSCRS FFSSHGLGVR AVAIEVEDAE SAFSISVANG AIPSSPPIVL NEAVTIAEVK 

       190        200        210        220        230        240 
LYGDVVLRYV SYKAEDTEKS EFLPGFERVE DASSFPLDYG IRRLDHAVGN VPELGPALTY 

       250        260        270        280        290        300 
VAGFTGFHQF AEFTADDVGT AESGLNSAVL ASNDEMVLLP INEPVHGTKR KSQIQTYLEH 

       310        320        330        340        350        360 
NEGAGLQHLA LMSEDIFRTL REMRKRSSIG GFDFMPSPPP TYYQNLKKRV GDVLSDDQIK 

       370        380        390        400        410        420 
ECEELGILVD RDDQGTLLQI FTKPLGDRPT IFIEIIQRVG CMMKDEEGKA YQSGGCGGFG 

       430        440 
KGNFSELFKS IEEYEKTLEA KQLVG 

« Hide

References

« Hide 'large scale' references
[1]"Cloning of an Arabidopsis thaliana cDNA for p-hydroxyphenylpyruvate dioxygenase."
Bartley G.E., Maxwell C.A., Wittenbach V.A., Scolnik P.A.
Plant Gene Register PGR97-065
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Wassilewskija.
[2]Norris S.R., Dellapenna D.
Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Columbia.
[3]"Characterization and subcellular compartmentation of recombinant 4-hydroxyphenylpyruvate dioxygenase from Arabidopsis in transgenic tobacco."
Garcia I., Rodgers M., Pepin R., Hsieh T.-F., Matringe M.
Plant Physiol. 119:1507-1516(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION.
Strain: cv. Columbia.
[4]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[5]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[6]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[7]"Structural basis for herbicidal inhibitor selectivity revealed by comparison of crystal structures of plant and mammalian 4-hydroxyphenylpyruvate dioxygenases."
Yang C., Pflugrath J.W., Camper D.L., Foster M.L., Pernich D.J., Walsh T.A.
Biochemistry 43:10414-10423(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 23-445 IN COMPLEX WITH IRON, COFACTOR, METAL-BINDING SITES, SUBUNIT.
[8]"The crystal structures of Zea mays and Arabidopsis 4-hydroxyphenylpyruvate dioxygenase."
Fritze I.M., Linden L., Freigang J., Auerbach G., Huber R., Steinbacher S.
Plant Physiol. 134:1388-1400(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS), SUBUNIT.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U89267 mRNA. Translation: AAB70025.1.
AF000228 mRNA. Translation: AAB58404.1.
AF047834 mRNA. Translation: AAC15697.1.
AC007592 Genomic DNA. Translation: AAF24813.1. Different initiation.
CP002684 Genomic DNA. Translation: AEE28006.1. Different initiation.
AF428446 mRNA. Translation: AAL16215.1.
AY072329 mRNA. Translation: AAL61936.1.
AY128745 mRNA. Translation: AAM91145.1.
IPIIPI00531512.
PIRB86201.
T51585.
RefSeqNP_172144.2. NM_100536.3.
UniGeneAt.48158.
At.71039.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1SP9X-ray3.00A/B1-445[»]
1SQDX-ray1.80A23-445[»]
1TFZX-ray1.80A23-445[»]
1TG5X-ray1.90A23-445[»]
ProteinModelPortalP93836.
SMRP93836. Positions 35-431.
ModBaseSearch...

Proteomic databases

PaxDbP93836.
PRIDEP93836.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID837168.
KEGGath:AT1G06570.

Organism-specific databases

TAIRAt1g06570.

Phylogenomic databases

eggNOGCOG3185.
InParanoidP93836.
KOK00457.
PhylomeDBP93836.
ProtClustDBPLN02875.

Enzyme and pathway databases

BRENDA1.13.11.27. 399.
UniPathwayUPA00139; UER00362.
UPA00975.

Gene expression databases

ArrayExpressP93836.
GenevestigatorP93836.
GermOnlineAT1G06570. Arabidopsis thaliana.

Family and domain databases

InterProIPR005956. 4OHPhenylPyrv_dOase.
IPR004360. Glyas_Fos-R_dOase_dom.
[Graphical view]
PANTHERPTHR11959. PTHR11959. 1 hit.
PfamPF00903. Glyoxalase. 1 hit.
[Graphical view]
PIRSFPIRSF009283. HPP_dOase. 1 hit.
TIGRFAMsTIGR01263. 4HPPD. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP93836.

Entry information

Entry nameHPPD_ARATH
AccessionPrimary (citable) accession number: P93836
Secondary accession number(s): F4IDP1, O04330, Q9SHK1
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: April 3, 2013
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families