P93836 (HPPD_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-hydroxyphenylpyruvate dioxygenase
EC=1.13.11.27

Alternative name(s):
4-hydroxyphenylpyruvic acid oxidase
Short name=4HPPD
Short name=HPD
Short name=HPPDase

Gene names

Name:	HPD
Synonyms:	PDS1
Ordered Locus Names:	At1g06570
ORF Names:	F12K11.9

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	445 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	4-hydroxyphenylpyruvate + O2 = homogentisate + CO2.
Cofactor	Binds 1 iron ion per subunit. Ref.7
Pathway	Amino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 3/6. Cofactor biosynthesis; prenylquinone biosynthesis.
Subunit structure	Homodimer. Ref.7 Ref.8
Subcellular location	Cytoplasm Ref.3.
Sequence similarities	Belongs to the 4HPPD family.
Sequence caution	The sequence AAF24813.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. The sequence AEE28006.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Biological process	Phenylalanine catabolism Tyrosine catabolism
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	L-phenylalanine catabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway tyrosine catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	4-hydroxyphenylpyruvate dioxygenase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		2	EBI-1251387,EBI-1251387

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: P93836-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 445

445

4-hydroxyphenylpyruvate dioxygenase

PRO_0000088397

Sites

Metal binding

226

1

Iron

Metal binding

308

1

Iron

Metal binding

394

1

Iron

Secondary structure

1

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445

Helix Strand Turn

Details...

Beta strand

44 – 53

10

Helix

57 – 68

12

Beta strand

71 – 77

7

Helix

78 – 80

3

Beta strand

83 – 92

10

Beta strand

95 – 102

8

Helix

105 – 107

3

Turn

108 – 110

3

Helix

113 – 115

3

Helix

125 – 135

11

Beta strand

137 – 147

11

Helix

149 – 158

10

Beta strand

163 – 170

8

Turn

171 – 173

3

Beta strand

174 – 182

9

Beta strand

185 – 192

8

Turn

211 – 213

3

Beta strand

219 – 230

12

Helix

234 – 245

12

Beta strand

248 – 253

6

Beta strand

265 – 271

7

Beta strand

277 – 284

8

Beta strand

287 – 290

4

Helix

293 – 301

9

Beta strand

305 – 314

10

Helix

316 – 326

11

Helix

327 – 329

3

Helix

340 – 350

11

Turn

351 – 353

3

Helix

356 – 365

10

Beta strand

368 – 371

4

Beta strand

373 – 382

10

Beta strand

385 – 389

5

Beta strand

392 – 400

9

Beta strand

406 – 408

3

Turn

414 – 417

4

Helix

423 – 430

8

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 1, 1998. Version 2. Checksum: FD0442F93556B3F5	FASTA	445	48,816
	10 20 30 40 50 60 MGHQNAAVSE NQNHDDGAAS SPGFKLVGFS KFVRKNPKSD KFKVKRFHHI EFWCGDATNV 70 80 90 100 110 120 ARRFSWGLGM RFSAKSDLST GNMVHASYLL TSGDLRFLFT APYSPSLSAG EIKPTTTASI 130 140 150 160 170 180 PSFDHGSCRS FFSSHGLGVR AVAIEVEDAE SAFSISVANG AIPSSPPIVL NEAVTIAEVK 190 200 210 220 230 240 LYGDVVLRYV SYKAEDTEKS EFLPGFERVE DASSFPLDYG IRRLDHAVGN VPELGPALTY 250 260 270 280 290 300 VAGFTGFHQF AEFTADDVGT AESGLNSAVL ASNDEMVLLP INEPVHGTKR KSQIQTYLEH 310 320 330 340 350 360 NEGAGLQHLA LMSEDIFRTL REMRKRSSIG GFDFMPSPPP TYYQNLKKRV GDVLSDDQIK 370 380 390 400 410 420 ECEELGILVD RDDQGTLLQI FTKPLGDRPT IFIEIIQRVG CMMKDEEGKA YQSGGCGGFG 430 440 KGNFSELFKS IEEYEKTLEA KQLVG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of an Arabidopsis thaliana cDNA for p-hydroxyphenylpyruvate dioxygenase." Bartley G.E., Maxwell C.A., Wittenbach V.A., Scolnik P.A. Plant Gene Register PGR97-065 Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Wassilewskija.
[2]	Norris S.R., Dellapenna D. Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[3]	"Characterization and subcellular compartmentation of recombinant 4-hydroxyphenylpyruvate dioxygenase from Arabidopsis in transgenic tobacco." Garcia I., Rodgers M., Pepin R., Hsieh T.-F., Matringe M. Plant Physiol. 119:1507-1516(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION. Strain: cv. Columbia.
[4]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[5]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[6]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[7]	"Structural basis for herbicidal inhibitor selectivity revealed by comparison of crystal structures of plant and mammalian 4-hydroxyphenylpyruvate dioxygenases." Yang C., Pflugrath J.W., Camper D.L., Foster M.L., Pernich D.J., Walsh T.A. Biochemistry 43:10414-10423(2004) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 23-445 IN COMPLEX WITH IRON, COFACTOR, METAL-BINDING SITES, SUBUNIT.
[8]	"The crystal structures of Zea mays and Arabidopsis 4-hydroxyphenylpyruvate dioxygenase." Fritze I.M., Linden L., Freigang J., Auerbach G., Huber R., Steinbacher S. Plant Physiol. 134:1388-1400(2004) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS), SUBUNIT.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U89267 mRNA. Translation: AAB70025.1.
AF000228 mRNA. Translation: AAB58404.1.
AF047834 mRNA. Translation: AAC15697.1.
AC007592 Genomic DNA. Translation: AAF24813.1. Different initiation.
CP002684 Genomic DNA. Translation: AEE28006.1. Different initiation.
AF428446 mRNA. Translation: AAL16215.1.
AY072329 mRNA. Translation: AAL61936.1.
AY128745 mRNA. Translation: AAM91145.1.

IPI

IPI00531512.

PIR

B86201.
T51585.

RefSeq

NP_172144.2. NM_100536.3.

UniGene

At.48158.
At.71039.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1SP9	X-ray	3.00	A/B	1-445	[»]
1SQD	X-ray	1.80	A	23-445	[»]
1TFZ	X-ray	1.80	A	23-445	[»]
1TG5	X-ray	1.90	A	23-445	[»]

ProteinModelPortal

P93836.

SMR

P93836. Positions 35-431.

ModBase

Search...

Proteomic databases

PaxDb

P93836.

PRIDE

P93836.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

837168.

KEGG

ath:AT1G06570.

Organism-specific databases

TAIR

At1g06570.

Phylogenomic databases

eggNOG

COG3185.

InParanoid

P93836.

KO

K00457.

PhylomeDB

P93836.

ProtClustDB

PLN02875.

Enzyme and pathway databases

BRENDA

1.13.11.27. 399.

UniPathway

UPA00139; UER00362.
UPA00975.

Gene expression databases

ArrayExpress

P93836.

Genevestigator

P93836.

GermOnline

AT1G06570. Arabidopsis thaliana.

Family and domain databases

InterPro

IPR005956. 4OHPhenylPyrv_dOase.
IPR004360. Glyas_Fos-R_dOase_dom.
[Graphical view]

PANTHER

PTHR11959. PTHR11959. 1 hit.

Pfam

PF00903. Glyoxalase. 1 hit.
[Graphical view]

PIRSF

PIRSF009283. HPP_dOase. 1 hit.

TIGRFAMs

TIGR01263. 4HPPD. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

P93836.

Entry information

Entry name

HPPD_ARATH

Accession

Primary (citable) accession number: P93836
Secondary accession number(s): F4IDP1, O04330, Q9SHK1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 1, 1998
Last modified:	April 3, 2013
This is version 110 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program