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P93834

- HXK2_ARATH

UniProt

P93834 - HXK2_ARATH

Protein

Hexokinase-2

Gene

HXK2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 1 (01 May 1997)
      Previous versions | rss
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    Functioni

    Fructose and glucose phosphorylating enzyme. May be involved in the phosphorylation of glucose during the export from mitochondrion to cytosol. Acts as sugar sensor which may regulate sugar-dependent gene repression or activation. Mediates the effects of sugar on plant growth and development independently of its catalytic activity or the sugar metabolism. May regulate the execution of program cell death in plant cells.2 Publications

    Catalytic activityi

    ATP + D-hexose = ADP + D-hexose 6-phosphate.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi101 – 1066ATPSequence Analysis

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. fructokinase activity Source: TAIR
    3. glucokinase activity Source: TAIR
    4. hexokinase activity Source: TAIR

    GO - Biological processi

    1. carbohydrate phosphorylation Source: GOC
    2. glucose 6-phosphate metabolic process Source: GOC
    3. glycolytic process Source: UniProtKB-KW
    4. hexokinase-dependent signaling Source: TAIR
    5. programmed cell death Source: TAIR
    6. sugar mediated signaling pathway Source: TAIR

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:GQT-1724-MONOMER.
    MetaCyc:AT2G19860-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hexokinase-2 (EC:2.7.1.1)
    Gene namesi
    Name:HXK2
    Ordered Locus Names:At2g19860
    ORF Names:F6F22.11
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G19860.

    Subcellular locationi

    Mitochondrion outer membrane 2 Publications; Single-pass membrane protein 2 Publications

    GO - Cellular componenti

    1. integral component of mitochondrial outer membrane Source: TAIR
    2. mitochondrion Source: TAIR
    3. plastid Source: TAIR

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion outer membrane

    Pathology & Biotechi

    Disruption phenotypei

    Plants are overall smaller with a reduced number of flowers and siliques and display a glucose-insensitive phenotype which allows them to grow on high glucose concentration medium (>6% glucose).1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 502502Hexokinase-2PRO_0000197613Add
    BLAST

    Proteomic databases

    PaxDbiP93834.
    PRIDEiP93834.

    Expressioni

    Tissue specificityi

    Highly expressed in siliques, at intermediate levels in roots and flowers, and at lower levels in stems, rosette and cauline leaves.1 Publication

    Gene expression databases

    ArrayExpressiP93834.
    GenevestigatoriP93834.

    Structurei

    3D structure databases

    ProteinModelPortaliP93834.
    SMRiP93834. Positions 55-487.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei4 – 2421HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini40 – 241202Hexokinase type-1Add
    BLAST
    Domaini246 – 488243Hexokinase type-2Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni171 – 19727Glucose-bindingSequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the hexokinase family.Curated
    Contains 1 hexokinase type-1 domain.Curated
    Contains 1 hexokinase type-2 domain.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG5026.
    HOGENOMiHOG000162670.
    InParanoidiP93834.
    KOiK00844.
    OMAiDFHLPPG.
    PhylomeDBiP93834.

    Family and domain databases

    InterProiIPR001312. Hexokinase.
    IPR022673. Hexokinase_C.
    IPR019807. Hexokinase_CS.
    IPR022672. Hexokinase_N.
    [Graphical view]
    PANTHERiPTHR19443. PTHR19443. 1 hit.
    PfamiPF00349. Hexokinase_1. 1 hit.
    PF03727. Hexokinase_2. 1 hit.
    [Graphical view]
    PRINTSiPR00475. HEXOKINASE.
    PROSITEiPS00378. HEXOKINASES. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    This entry describes 1 isoform i produced by alternative splicing. Align

    Note: A number of isoforms are produced. According to EST sequences.

    Isoform 1 (identifier: P93834-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

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    MGKVAVATTV VCSVAVCAAA ALIVRRRMKS AGKWARVIEI LKAFEEDCAT    50
    PIAKLRQVAD AMTVEMHAGL ASEGGSKLKM LISYVDNLPS GDETGFFYAL 100
    DLGGTNFRVM RVLLGGKHDR VVKREFKEES IPPHLMTGKS HELFDFIVDV 150
    LAKFVATEGE DFHLPPGRQR ELGFTFSFPV KQLSLSSGTL INWTKGFSID 200
    DTVDKDVVGE LVKAMERVGL DMLVAALVND TIGTLAGGRY TNPDVVVAVI 250
    LGTGTNAAYV ERAHAIPKWH GLLPKSGEMV INMEWGNFRS SHLPLTEYDH 300
    SLDVDSLNPG EQILEKIISG MYLGEILRRV LLKMAEEAAF FGDIVPPKLK 350
    IPFIIRTPNM SAMHSDTSPD LKVVGSKLKD ILEVQTSSLK MRKVVISLCN 400
    IIASRGARLS AAGIYGILKK IGRDATKDGE AQKSVIAMDG GLFEHYTQFS 450
    ESMKSSLKEL LGDEVSESVE VILSNDGSGV GAALLAASHS QYLELEDDSE 500
    TS 502
    Length:502
    Mass (Da):54,490
    Last modified:May 1, 1997 - v1
    Checksum:iFEAF0B860D7531C9
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti242 – 2421N → S in AAO24584. (PubMed:14593172)Curated
    Sequence conflicti242 – 2421N → S in BAE99655. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U28215 mRNA. Translation: AAB49911.1.
    AC005169 Genomic DNA. Translation: AAC62130.1.
    CP002685 Genomic DNA. Translation: AEC06934.1.
    BT003152 mRNA. Translation: AAO24584.1.
    AK227668 mRNA. Translation: BAE99655.1.
    PIRiA84582.
    RefSeqiNP_179576.1. NM_127544.2. [P93834-1]
    UniGeneiAt.12899.

    Genome annotation databases

    EnsemblPlantsiAT2G19860.1; AT2G19860.1; AT2G19860. [P93834-1]
    GeneIDi816505.
    KEGGiath:AT2G19860.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U28215 mRNA. Translation: AAB49911.1 .
    AC005169 Genomic DNA. Translation: AAC62130.1 .
    CP002685 Genomic DNA. Translation: AEC06934.1 .
    BT003152 mRNA. Translation: AAO24584.1 .
    AK227668 mRNA. Translation: BAE99655.1 .
    PIRi A84582.
    RefSeqi NP_179576.1. NM_127544.2. [P93834-1 ]
    UniGenei At.12899.

    3D structure databases

    ProteinModelPortali P93834.
    SMRi P93834. Positions 55-487.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PaxDbi P93834.
    PRIDEi P93834.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G19860.1 ; AT2G19860.1 ; AT2G19860 . [P93834-1 ]
    GeneIDi 816505.
    KEGGi ath:AT2G19860.

    Organism-specific databases

    GeneFarmi 4269. 430.
    TAIRi AT2G19860.

    Phylogenomic databases

    eggNOGi COG5026.
    HOGENOMi HOG000162670.
    InParanoidi P93834.
    KOi K00844.
    OMAi DFHLPPG.
    PhylomeDBi P93834.

    Enzyme and pathway databases

    BioCyci ARA:GQT-1724-MONOMER.
    MetaCyc:AT2G19860-MONOMER.

    Gene expression databases

    ArrayExpressi P93834.
    Genevestigatori P93834.

    Family and domain databases

    InterProi IPR001312. Hexokinase.
    IPR022673. Hexokinase_C.
    IPR019807. Hexokinase_CS.
    IPR022672. Hexokinase_N.
    [Graphical view ]
    PANTHERi PTHR19443. PTHR19443. 1 hit.
    Pfami PF00349. Hexokinase_1. 1 hit.
    PF03727. Hexokinase_2. 1 hit.
    [Graphical view ]
    PRINTSi PR00475. HEXOKINASE.
    PROSITEi PS00378. HEXOKINASES. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Hexokinase as a sugar sensor in higher plants."
      Jang J.-C., Leon P., Zhou L., Sheen J.
      Plant Cell 9:5-19(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
      Strain: cv. Landsberg erecta.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
      Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
      , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
      Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    6. "Enzymes of glycolysis are functionally associated with the mitochondrion in Arabidopsis cells."
      Giege P., Heazlewood J.L., Roessner-Tunali U., Millar A.H., Fernie A.R., Leaver C.J., Sweetlove L.J.
      Plant Cell 15:2140-2151(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    7. "Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins."
      Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H.
      Plant Cell 16:241-256(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION.
      Strain: cv. Landsberg erecta.
    8. "Mitochondria-associated hexokinases play a role in the control of programmed cell death in Nicotiana benthamiana."
      Kim M., Lim J.-H., Ahn C.S., Park K., Kim G.T., Kim W.T., Pai H.-S.
      Plant Cell 18:2341-2355(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiHXK2_ARATH
    AccessioniPrimary (citable) accession number: P93834
    Secondary accession number(s): Q0WT93, Q84WJ5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: May 1, 1997
    Last modified: October 1, 2014
    This is version 116 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3