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Protein

Hexokinase-2

Gene

HXK2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Fructose and glucose phosphorylating enzyme. May be involved in the phosphorylation of glucose during the export from mitochondrion to cytosol. Acts as sugar sensor which may regulate sugar-dependent gene repression or activation. Mediates the effects of sugar on plant growth and development independently of its catalytic activity or the sugar metabolism. May regulate the execution of program cell death in plant cells.2 Publications

Catalytic activityi

ATP + D-hexose = ADP + D-hexose 6-phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi101 – 1066ATPSequence analysis

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • fructokinase activity Source: TAIR
  • glucokinase activity Source: TAIR
  • glucose binding Source: InterPro
  • hexokinase activity Source: TAIR
  • mannokinase activity Source: GO_Central

GO - Biological processi

  • cellular glucose homeostasis Source: GO_Central
  • glycolytic process Source: GO_Central
  • hexokinase-dependent signaling Source: TAIR
  • programmed cell death Source: TAIR
  • sugar mediated signaling pathway Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:GQT-1724-MONOMER.
MetaCyc:AT2G19860-MONOMER.
ReactomeiR-ATH-170822. Regulation of Glucokinase by Glucokinase Regulatory Protein.
R-ATH-70153. Glucose transport.
R-ATH-70171. Glycolysis.

Names & Taxonomyi

Protein namesi
Recommended name:
Hexokinase-2 (EC:2.7.1.1)
Gene namesi
Name:HXK2
Ordered Locus Names:At2g19860
ORF Names:F6F22.11
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G19860.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei4 – 2421HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • cytosol Source: GO_Central
  • integral component of mitochondrial outer membrane Source: TAIR
  • mitochondrion Source: TAIR
  • plastid Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion outer membrane

Pathology & Biotechi

Disruption phenotypei

Plants are overall smaller with a reduced number of flowers and siliques and display a glucose-insensitive phenotype which allows them to grow on high glucose concentration medium (>6% glucose).1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 502502Hexokinase-2PRO_0000197613Add
BLAST

Proteomic databases

PaxDbiP93834.
PRIDEiP93834.

Expressioni

Tissue specificityi

Highly expressed in siliques, at intermediate levels in roots and flowers, and at lower levels in stems, rosette and cauline leaves.1 Publication

Gene expression databases

ExpressionAtlasiP93834. baseline and differential.
GenevisibleiP93834. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G19860.1.

Structurei

3D structure databases

ProteinModelPortaliP93834.
SMRiP93834. Positions 32-493.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini35 – 487453HexokinasePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni90 – 228139Hexokinase small subdomainPROSITE-ProRule annotationAdd
BLAST
Regioni171 – 19727Glucose-bindingSequence analysisAdd
BLAST
Regioni229 – 476248Hexokinase large subdomainPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the hexokinase family.PROSITE-ProRule annotationCurated
Contains 1 hexokinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1369. Eukaryota.
COG5026. LUCA.
HOGENOMiHOG000162670.
InParanoidiP93834.
KOiK00844.
OMAiTGKSHEL.
OrthoDBiEOG093608SG.
PhylomeDBiP93834.

Family and domain databases

InterProiIPR001312. Hexokinase.
IPR019807. Hexokinase_BS.
IPR022673. Hexokinase_C.
IPR022672. Hexokinase_N.
[Graphical view]
PANTHERiPTHR19443. PTHR19443. 1 hit.
PfamiPF00349. Hexokinase_1. 1 hit.
PF03727. Hexokinase_2. 1 hit.
[Graphical view]
PROSITEiPS00378. HEXOKINASE_1. 1 hit.
PS51748. HEXOKINASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: P93834-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGKVAVATTV VCSVAVCAAA ALIVRRRMKS AGKWARVIEI LKAFEEDCAT
60 70 80 90 100
PIAKLRQVAD AMTVEMHAGL ASEGGSKLKM LISYVDNLPS GDETGFFYAL
110 120 130 140 150
DLGGTNFRVM RVLLGGKHDR VVKREFKEES IPPHLMTGKS HELFDFIVDV
160 170 180 190 200
LAKFVATEGE DFHLPPGRQR ELGFTFSFPV KQLSLSSGTL INWTKGFSID
210 220 230 240 250
DTVDKDVVGE LVKAMERVGL DMLVAALVND TIGTLAGGRY TNPDVVVAVI
260 270 280 290 300
LGTGTNAAYV ERAHAIPKWH GLLPKSGEMV INMEWGNFRS SHLPLTEYDH
310 320 330 340 350
SLDVDSLNPG EQILEKIISG MYLGEILRRV LLKMAEEAAF FGDIVPPKLK
360 370 380 390 400
IPFIIRTPNM SAMHSDTSPD LKVVGSKLKD ILEVQTSSLK MRKVVISLCN
410 420 430 440 450
IIASRGARLS AAGIYGILKK IGRDATKDGE AQKSVIAMDG GLFEHYTQFS
460 470 480 490 500
ESMKSSLKEL LGDEVSESVE VILSNDGSGV GAALLAASHS QYLELEDDSE

TS
Length:502
Mass (Da):54,490
Last modified:May 1, 1997 - v1
Checksum:iFEAF0B860D7531C9
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti242 – 2421N → S in AAO24584 (PubMed:14593172).Curated
Sequence conflicti242 – 2421N → S in BAE99655 (Ref. 5) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28215 mRNA. Translation: AAB49911.1.
AC005169 Genomic DNA. Translation: AAC62130.1.
CP002685 Genomic DNA. Translation: AEC06934.1.
BT003152 mRNA. Translation: AAO24584.1.
AK227668 mRNA. Translation: BAE99655.1.
PIRiA84582.
RefSeqiNP_179576.1. NM_127544.2. [P93834-1]
UniGeneiAt.12899.

Genome annotation databases

EnsemblPlantsiAT2G19860.1; AT2G19860.1; AT2G19860. [P93834-1]
GeneIDi816505.
KEGGiath:AT2G19860.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28215 mRNA. Translation: AAB49911.1.
AC005169 Genomic DNA. Translation: AAC62130.1.
CP002685 Genomic DNA. Translation: AEC06934.1.
BT003152 mRNA. Translation: AAO24584.1.
AK227668 mRNA. Translation: BAE99655.1.
PIRiA84582.
RefSeqiNP_179576.1. NM_127544.2. [P93834-1]
UniGeneiAt.12899.

3D structure databases

ProteinModelPortaliP93834.
SMRiP93834. Positions 32-493.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT2G19860.1.

Proteomic databases

PaxDbiP93834.
PRIDEiP93834.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G19860.1; AT2G19860.1; AT2G19860. [P93834-1]
GeneIDi816505.
KEGGiath:AT2G19860.

Organism-specific databases

TAIRiAT2G19860.

Phylogenomic databases

eggNOGiKOG1369. Eukaryota.
COG5026. LUCA.
HOGENOMiHOG000162670.
InParanoidiP93834.
KOiK00844.
OMAiTGKSHEL.
OrthoDBiEOG093608SG.
PhylomeDBiP93834.

Enzyme and pathway databases

BioCyciARA:GQT-1724-MONOMER.
MetaCyc:AT2G19860-MONOMER.
ReactomeiR-ATH-170822. Regulation of Glucokinase by Glucokinase Regulatory Protein.
R-ATH-70153. Glucose transport.
R-ATH-70171. Glycolysis.

Miscellaneous databases

PROiP93834.

Gene expression databases

ExpressionAtlasiP93834. baseline and differential.
GenevisibleiP93834. AT.

Family and domain databases

InterProiIPR001312. Hexokinase.
IPR019807. Hexokinase_BS.
IPR022673. Hexokinase_C.
IPR022672. Hexokinase_N.
[Graphical view]
PANTHERiPTHR19443. PTHR19443. 1 hit.
PfamiPF00349. Hexokinase_1. 1 hit.
PF03727. Hexokinase_2. 1 hit.
[Graphical view]
PROSITEiPS00378. HEXOKINASE_1. 1 hit.
PS51748. HEXOKINASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHXK2_ARATH
AccessioniPrimary (citable) accession number: P93834
Secondary accession number(s): Q0WT93, Q84WJ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 1997
Last modified: September 7, 2016
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.