Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P93834

- HXK2_ARATH

UniProt

P93834 - HXK2_ARATH

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Hexokinase-2

Gene

HXK2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Fructose and glucose phosphorylating enzyme. May be involved in the phosphorylation of glucose during the export from mitochondrion to cytosol. Acts as sugar sensor which may regulate sugar-dependent gene repression or activation. Mediates the effects of sugar on plant growth and development independently of its catalytic activity or the sugar metabolism. May regulate the execution of program cell death in plant cells.2 Publications

Catalytic activityi

ATP + D-hexose = ADP + D-hexose 6-phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi101 – 1066ATPSequence Analysis

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. fructokinase activity Source: TAIR
  3. glucokinase activity Source: TAIR
  4. hexokinase activity Source: TAIR

GO - Biological processi

  1. carbohydrate phosphorylation Source: GOC
  2. glucose 6-phosphate metabolic process Source: GOC
  3. glycolytic process Source: UniProtKB-KW
  4. hexokinase-dependent signaling Source: TAIR
  5. programmed cell death Source: TAIR
  6. sugar mediated signaling pathway Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:GQT-1724-MONOMER.
MetaCyc:AT2G19860-MONOMER.
ReactomeiREACT_234283. Regulation of Glucokinase by Glucokinase Regulatory Protein.
REACT_239741. Regulation of gene expression in beta cells.
REACT_253266. Glucose transport.

Names & Taxonomyi

Protein namesi
Recommended name:
Hexokinase-2 (EC:2.7.1.1)
Gene namesi
Name:HXK2
Ordered Locus Names:At2g19860
ORF Names:F6F22.11
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G19860.

Subcellular locationi

Mitochondrion outer membrane 2 Publications; Single-pass membrane protein 2 Publications

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei4 – 2421HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of mitochondrial outer membrane Source: TAIR
  2. mitochondrion Source: TAIR
  3. plastid Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion outer membrane

Pathology & Biotechi

Disruption phenotypei

Plants are overall smaller with a reduced number of flowers and siliques and display a glucose-insensitive phenotype which allows them to grow on high glucose concentration medium (>6% glucose).1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 502502Hexokinase-2PRO_0000197613Add
BLAST

Proteomic databases

PaxDbiP93834.
PRIDEiP93834.

Expressioni

Tissue specificityi

Highly expressed in siliques, at intermediate levels in roots and flowers, and at lower levels in stems, rosette and cauline leaves.1 Publication

Gene expression databases

ExpressionAtlasiP93834. baseline and differential.
GenevestigatoriP93834.

Structurei

3D structure databases

ProteinModelPortaliP93834.
SMRiP93834. Positions 55-487.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini40 – 241202Hexokinase type-1Add
BLAST
Domaini246 – 488243Hexokinase type-2Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni171 – 19727Glucose-bindingSequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the hexokinase family.Curated
Contains 1 hexokinase type-1 domain.Curated
Contains 1 hexokinase type-2 domain.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG5026.
HOGENOMiHOG000162670.
InParanoidiP93834.
KOiK00844.
OMAiDFHLPPG.
PhylomeDBiP93834.

Family and domain databases

InterProiIPR001312. Hexokinase.
IPR022673. Hexokinase_C.
IPR019807. Hexokinase_CS.
IPR022672. Hexokinase_N.
[Graphical view]
PANTHERiPTHR19443. PTHR19443. 1 hit.
PfamiPF00349. Hexokinase_1. 1 hit.
PF03727. Hexokinase_2. 1 hit.
[Graphical view]
PRINTSiPR00475. HEXOKINASE.
PROSITEiPS00378. HEXOKINASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. Align

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: P93834-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGKVAVATTV VCSVAVCAAA ALIVRRRMKS AGKWARVIEI LKAFEEDCAT
60 70 80 90 100
PIAKLRQVAD AMTVEMHAGL ASEGGSKLKM LISYVDNLPS GDETGFFYAL
110 120 130 140 150
DLGGTNFRVM RVLLGGKHDR VVKREFKEES IPPHLMTGKS HELFDFIVDV
160 170 180 190 200
LAKFVATEGE DFHLPPGRQR ELGFTFSFPV KQLSLSSGTL INWTKGFSID
210 220 230 240 250
DTVDKDVVGE LVKAMERVGL DMLVAALVND TIGTLAGGRY TNPDVVVAVI
260 270 280 290 300
LGTGTNAAYV ERAHAIPKWH GLLPKSGEMV INMEWGNFRS SHLPLTEYDH
310 320 330 340 350
SLDVDSLNPG EQILEKIISG MYLGEILRRV LLKMAEEAAF FGDIVPPKLK
360 370 380 390 400
IPFIIRTPNM SAMHSDTSPD LKVVGSKLKD ILEVQTSSLK MRKVVISLCN
410 420 430 440 450
IIASRGARLS AAGIYGILKK IGRDATKDGE AQKSVIAMDG GLFEHYTQFS
460 470 480 490 500
ESMKSSLKEL LGDEVSESVE VILSNDGSGV GAALLAASHS QYLELEDDSE

TS
Length:502
Mass (Da):54,490
Last modified:May 1, 1997 - v1
Checksum:iFEAF0B860D7531C9
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti242 – 2421N → S in AAO24584. (PubMed:14593172)Curated
Sequence conflicti242 – 2421N → S in BAE99655. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28215 mRNA. Translation: AAB49911.1.
AC005169 Genomic DNA. Translation: AAC62130.1.
CP002685 Genomic DNA. Translation: AEC06934.1.
BT003152 mRNA. Translation: AAO24584.1.
AK227668 mRNA. Translation: BAE99655.1.
PIRiA84582.
RefSeqiNP_179576.1. NM_127544.2. [P93834-1]
UniGeneiAt.12899.

Genome annotation databases

EnsemblPlantsiAT2G19860.1; AT2G19860.1; AT2G19860. [P93834-1]
GeneIDi816505.
KEGGiath:AT2G19860.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28215 mRNA. Translation: AAB49911.1 .
AC005169 Genomic DNA. Translation: AAC62130.1 .
CP002685 Genomic DNA. Translation: AEC06934.1 .
BT003152 mRNA. Translation: AAO24584.1 .
AK227668 mRNA. Translation: BAE99655.1 .
PIRi A84582.
RefSeqi NP_179576.1. NM_127544.2. [P93834-1 ]
UniGenei At.12899.

3D structure databases

ProteinModelPortali P93834.
SMRi P93834. Positions 55-487.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PaxDbi P93834.
PRIDEi P93834.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G19860.1 ; AT2G19860.1 ; AT2G19860 . [P93834-1 ]
GeneIDi 816505.
KEGGi ath:AT2G19860.

Organism-specific databases

GeneFarmi 4269. 430.
TAIRi AT2G19860.

Phylogenomic databases

eggNOGi COG5026.
HOGENOMi HOG000162670.
InParanoidi P93834.
KOi K00844.
OMAi DFHLPPG.
PhylomeDBi P93834.

Enzyme and pathway databases

BioCyci ARA:GQT-1724-MONOMER.
MetaCyc:AT2G19860-MONOMER.
Reactomei REACT_234283. Regulation of Glucokinase by Glucokinase Regulatory Protein.
REACT_239741. Regulation of gene expression in beta cells.
REACT_253266. Glucose transport.

Gene expression databases

ExpressionAtlasi P93834. baseline and differential.
Genevestigatori P93834.

Family and domain databases

InterProi IPR001312. Hexokinase.
IPR022673. Hexokinase_C.
IPR019807. Hexokinase_CS.
IPR022672. Hexokinase_N.
[Graphical view ]
PANTHERi PTHR19443. PTHR19443. 1 hit.
Pfami PF00349. Hexokinase_1. 1 hit.
PF03727. Hexokinase_2. 1 hit.
[Graphical view ]
PRINTSi PR00475. HEXOKINASE.
PROSITEi PS00378. HEXOKINASES. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Hexokinase as a sugar sensor in higher plants."
    Jang J.-C., Leon P., Zhou L., Sheen J.
    Plant Cell 9:5-19(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
    Strain: cv. Landsberg erecta.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "Enzymes of glycolysis are functionally associated with the mitochondrion in Arabidopsis cells."
    Giege P., Heazlewood J.L., Roessner-Tunali U., Millar A.H., Fernie A.R., Leaver C.J., Sweetlove L.J.
    Plant Cell 15:2140-2151(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  7. "Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins."
    Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H.
    Plant Cell 16:241-256(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION.
    Strain: cv. Landsberg erecta.
  8. "Mitochondria-associated hexokinases play a role in the control of programmed cell death in Nicotiana benthamiana."
    Kim M., Lim J.-H., Ahn C.S., Park K., Kim G.T., Kim W.T., Pai H.-S.
    Plant Cell 18:2341-2355(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiHXK2_ARATH
AccessioniPrimary (citable) accession number: P93834
Secondary accession number(s): Q0WT93, Q84WJ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 1997
Last modified: November 26, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3