Reviewed,
UniProtKB/Swiss-Prot P93832 (LEU32_ARATH)
Last modified
November 25, 2008.
Version 65.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: 3-isopropylmalate dehydrogenase 2, chloroplastic Short name=3-IPM-DH 2 Short name=IMDH 2 EC=1.1.1.85 Alternative name(s): Beta-IPM dehydrogenase 2 | ||||||||
| Gene names |
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| Organism | Arabidopsis thaliana (Mouse-ear cress) [Complete proteome] | ||||||||
| Taxonomic identifier | 3702 [NCBI] | ||||||||
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis |
Protein attributes
| Sequence length | 405 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Function | Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate. |
| Catalytic activity | (2R,3S)-3-isopropylmalate + NAD(+) = 4-methyl-2-oxopentanoate + CO(2) + NADH. |
| Cofactor | Binds 1 magnesium or manganese ion per subunit By similarity. |
| Pathway | Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 3/4. |
| Subunit structure | Homodimer By similarity. |
| Subcellular location | Plastid › chloroplastBy similarity. |
| Sequence similarities | Belongs to the isocitrate and isopropylmalate dehydrogenases family. |
Ontologies
Keywords | |
|---|---|
| Biological process | Amino-acid biosynthesis Branched-chain amino acid biosynthesis Leucine biosynthesis |
| Cellular component | Chloroplast Plastid |
| Domain | Transit peptide |
| Ligand | Magnesium Manganese Metal-binding NAD |
| Molecular function | Oxidoreductase |
| Technical term | Complete proteome |
Gene Ontology (GO) | |
| Biological process | leucine biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reductionInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | chloroplast Inferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | 3-isopropylmalate dehydrogenase activity Inferred from electronic annotation. Source: InterPro magnesium ion bindingInferred from electronic annotation. Source: UniProtKB-KW manganese ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Transit peptide | 1 – 33 | 33 | Chloroplast Potential | ||||||
| Chain | 34 – 405 | 372 | 3-isopropylmalate dehydrogenase 2, chloroplastic | PRO_0000014454 | |||||
Regions | |||||||||
| Nucleotide binding | 116 – 129 | 14 | NAD By similarity | ||||||
| Nucleotide binding | 322 – 334 | 13 | NAD By similarity | ||||||
Sites | |||||||||
| Metal binding | 264 | 1 | Magnesium or manganese By similarity | ||||||
| Metal binding | 288 | 1 | Magnesium or manganese By similarity | ||||||
| Metal binding | 292 | 1 | Magnesium or manganese By similarity | ||||||
| Binding site | 136 | 1 | Substrate By similarity | ||||||
| Binding site | 146 | 1 | Substrate By similarity | ||||||
| Binding site | 174 | 1 | Substrate By similarity | ||||||
| Binding site | 264 | 1 | Substrate By similarity | ||||||
| Site | 181 | 1 | Important for catalysis By similarity | ||||||
| Site | 232 | 1 | Important for catalysis By similarity | ||||||
Experimental info | |||||||||
| Sequence conflict | 177 | 1 | T → K in AAL32519 and AAM47946. Ref.3 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | Salchert K.D. Submitted (DEC-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia. |
| [2] | "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. Davis R.W.Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia. |
| [3] | "Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R.Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia. |
Cross-references
Sequence databases | |
|---|---|
| Y10216 mRNA. Translation: CAA71268.1. AC018849 Genomic DNA. Translation: AAF27137.1. AY062441 mRNA. Translation: AAL32519.1. AY114627 mRNA. Translation: AAM47946.1. | |
| PIR | F96837. |
| RefSeq | NP_178171.1. |
| UniGene | At.5371 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1A05 based on UniProtKB Q56268. |
| ModBase | Search... |
Genome annotation databases | |
| GeneID | 844395. |
| GenomeReviews | Gene locus AT1G80560 in contig CT485782_GR. |
| KEGG | ath:AT1G80560. |
| NMPDR | fig|3702.1.peg.7645. |
Organism-specific databases | |
| TAIR | At1g80560. |
Gene expression databases | |
| ArrayExpress | P93832. |
| GermOnline | AT1G80560. Arabidopsis thaliana. |
Family and domain databases | |
| InterPro | IPR004429. 3-isopropylmalate_DHase. IPR001804. IsoCit_IM_DHase. [Graphical view] |
| Gene3D | G3DSA:3.40.718.10. IDH_IMDH. 1 hit. |
| PANTHER | PTHR11835. IDH_IMDH_dimeric. 1 hit. PTHR11835:SF13. IPMDH. 1 hit. |
| Pfam | PF00180. Iso_dh. 1 hit. [Graphical view] |
| TIGRFAMs | TIGR00169. leuB. 1 hit. |
| PROSITE | PS00470. IDH_IMDH. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | LEU32_ARATH | ||||||||
| Accession | Primary (citable) accession number: P93832 Secondary accession number(s): Q540Z4, Q8W4P6 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | PPAP (Plant Proteome Annotation Project) | ||||||||
Relevant documents
| Arabidopsis thaliana Arabidopsis thaliana: entries and gene names |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with


