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P93819

- MDHC1_ARATH

UniProt

P93819 - MDHC1_ARATH

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Protein
Malate dehydrogenase, cytoplasmic 1
Gene
At1g04410, F19P19.13
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei93 – 931Substrate By similarity
Binding sitei99 – 991Substrate By similarity
Binding sitei106 – 1061NAD By similarity
Binding sitei113 – 1131NAD By similarity
Binding sitei132 – 1321Substrate By similarity
Binding sitei163 – 1631Substrate By similarity
Active sitei188 – 1881Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi12 – 187NAD By similarity
Nucleotide bindingi130 – 1323NAD By similarity

GO - Molecular functioni

  1. L-malate dehydrogenase activity Source: UniProtKB-EC

GO - Biological processi

  1. cellular carbohydrate metabolic process Source: InterPro
  2. malate metabolic process Source: InterPro
  3. response to cadmium ion Source: TAIR
  4. response to salt stress Source: TAIR
  5. response to zinc ion Source: TAIR
  6. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenase, cytoplasmic 1 (EC:1.1.1.37)
Gene namesi
Ordered Locus Names:At1g04410
ORF Names:F19P19.13
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G04410.

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. apoplast Source: TAIR
  2. chloroplast Source: TAIR
  3. chloroplast stroma Source: TAIR
  4. membrane Source: TAIR
  5. nucleus Source: TAIR
  6. plasma membrane Source: TAIR
  7. plasmodesma Source: TAIR
  8. vacuolar membrane Source: TAIR
  9. vacuole Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 332332Malate dehydrogenase, cytoplasmic 1
PRO_0000113412Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki119 – 119Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication

Post-translational modificationi

Ubiquitinated.

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiP93819.
PRIDEiP93819.

2D gel databases

SWISS-2DPAGEP93819.

Expressioni

Gene expression databases

ArrayExpressiP93819.
GenevestigatoriP93819.

Interactioni

Subunit structurei

Homodimer By similarity.

Protein-protein interaction databases

BioGridi24762. 9 interactions.
IntActiP93819. 4 interactions.
STRINGi3702.AT1G04410.1-P.

Structurei

3D structure databases

ProteinModelPortaliP93819.
SMRiP93819. Positions 4-332.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0039.
HOGENOMiHOG000220953.
InParanoidiP93819.
KOiK00025.
OMAiNCLIASK.
PhylomeDBiP93819.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR011274. Malate_DH_NAD-dep_euk.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23382. PTHR23382. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01759. MalateDH-SF1. 1 hit.
TIGR01758. MDH_euk_cyt. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P93819-1 [UniParc]FASTAAdd to Basket

« Hide

MAKEPVRVLV TGAAGQIGYA LVPMIARGIM LGADQPVILH MLDIPPAAEA    50
LNGVKMELID AAFPLLKGVV ATTDAVEGCT GVNVAVMVGG FPRKEGMERK 100
DVMSKNVSIY KSQAAALEKH AAPNCKVLVV ANPANTNALI LKEFAPSIPE 150
KNISCLTRLD HNRALGQISE RLSVPVSDVK NVIIWGNHSS SQYPDVNHAK 200
VQTSSGEKPV RELVKDDAWL DGEFISTVQQ RGAAIIKARK LSSALSAASS 250
ACDHIRDWVL GTPEGTFVSM GVYSDGSYSV PSGLIYSFPV TCRNGDWSIV 300
QGLPIDEVSR KKMDLTAEEL KEEKDLAYSC LS 332
Length:332
Mass (Da):35,571
Last modified:January 1, 1998 - v2
Checksum:iC85D11E2BDFF556D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC000104 Genomic DNA. Translation: AAB70434.1.
CP002684 Genomic DNA. Translation: AEE27694.1.
AY050374 mRNA. Translation: AAK91392.1.
AY065134 mRNA. Translation: AAL38310.1.
AY081563 mRNA. Translation: AAM10125.1.
AY133655 mRNA. Translation: AAM91485.1.
AY088023 mRNA. Translation: AAM65569.1.
PIRiB86176.
RefSeqiNP_171936.1. NM_100321.2.
UniGeneiAt.11041.
At.47261.

Genome annotation databases

EnsemblPlantsiAT1G04410.1; AT1G04410.1; AT1G04410.
GeneIDi839527.
KEGGiath:AT1G04410.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC000104 Genomic DNA. Translation: AAB70434.1 .
CP002684 Genomic DNA. Translation: AEE27694.1 .
AY050374 mRNA. Translation: AAK91392.1 .
AY065134 mRNA. Translation: AAL38310.1 .
AY081563 mRNA. Translation: AAM10125.1 .
AY133655 mRNA. Translation: AAM91485.1 .
AY088023 mRNA. Translation: AAM65569.1 .
PIRi B86176.
RefSeqi NP_171936.1. NM_100321.2.
UniGenei At.11041.
At.47261.

3D structure databases

ProteinModelPortali P93819.
SMRi P93819. Positions 4-332.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 24762. 9 interactions.
IntActi P93819. 4 interactions.
STRINGi 3702.AT1G04410.1-P.

2D gel databases

SWISS-2DPAGE P93819.

Proteomic databases

PaxDbi P93819.
PRIDEi P93819.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G04410.1 ; AT1G04410.1 ; AT1G04410 .
GeneIDi 839527.
KEGGi ath:AT1G04410.

Organism-specific databases

TAIRi AT1G04410.

Phylogenomic databases

eggNOGi COG0039.
HOGENOMi HOG000220953.
InParanoidi P93819.
KOi K00025.
OMAi NCLIASK.
PhylomeDBi P93819.

Gene expression databases

ArrayExpressi P93819.
Genevestigatori P93819.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProi IPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR011274. Malate_DH_NAD-dep_euk.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
PANTHERi PTHR23382. PTHR23382. 1 hit.
Pfami PF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMi SSF56327. SSF56327. 1 hit.
TIGRFAMsi TIGR01759. MalateDH-SF1. 1 hit.
TIGR01758. MDH_euk_cyt. 1 hit.
PROSITEi PS00068. MDH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. "Tandem affinity purification and mass spectrometric analysis of ubiquitylated proteins in Arabidopsis."
    Saracco S.A., Hansson M., Scalf M., Walker J.M., Smith L.M., Vierstra R.D.
    Plant J. 59:344-358(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-119.

Entry informationi

Entry nameiMDHC1_ARATH
AccessioniPrimary (citable) accession number: P93819
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: January 1, 1998
Last modified: May 14, 2014
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi