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P93771 (GAOX1_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Gibberellin 20 oxidase 1

EC=1.14.11.-
Alternative name(s):
GA 20-oxidase 1
Gibberellin C-20 oxidase 1
Os20ox
Gene names
Name:20ox1
Synonyms:GA20ox-1
Ordered Locus Names:Os03g0856700, LOC_Os03g63970
ORF Names:OSJNBa0059G06.22
OrganismOryza sativa subsp. japonica (Rice) [Reference proteome]
Taxonomic identifier39947 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza

Protein attributes

Sequence length372 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Key oxidase enzyme in the biosynthesis of gibberellin that catalyzes the conversion of GA12 and GA53 to GA9 and GA20 respectively, via a three-step oxidation at C-20 of the GA skeleton. Ref.1

Cofactor

Iron.

Ascorbate.

2-oxoglutarate.

Tissue specificity

Preferentially expressed in reproductive organs. Ref.3

Induction

Negatively controlled by the level of physiologically active gibberellin. Ref.1

Sequence similarities

Belongs to the iron/ascorbate-dependent oxidoreductase family. GA20OX subfamily.

Contains 1 Fe2OG dioxygenase domain.

Sequence caution

The sequence AAP21386.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 372372Gibberellin 20 oxidase 1
PRO_0000219517

Regions

Domain209 – 309101Fe2OG dioxygenase

Sites

Active site3001 Potential
Metal binding2341Iron By similarity
Metal binding2361Iron By similarity
Metal binding2901Iron By similarity

Experimental info

Sequence conflict107 – 11610AQRRAGESCG → RSGARGRTA in AAB48239. Ref.1
Sequence conflict3521L → F Ref.1

Sequences

Sequence LengthMass (Da)Tools
P93771 [UniParc].

Last modified March 1, 2005. Version 2.
Checksum: 6BC53284017F9C27

FASTA37242,302
        10         20         30         40         50         60 
MSMVVQQEQE VVFDAAVLSG QTEIPSQFIW PAEESPGSVA VEELEVALID VGAGAERSSV 

        70         80         90        100        110        120 
VRQVGEACER HGFFLVVNHG IEAALLEEAH RCMDAFFTLP LGEKQRAQRR AGESCGYASS 

       130        140        150        160        170        180 
FTGRFASKLP WKETLSFRYS SAGDEEGEEG VGEYLVRKLG AEHGRRLGEV YSRYCHEMSR 

       190        200        210        220        230        240 
LSLELMEVLG ESLGIVGDRR HYFRRFFQRN DSIMRLNYYP ACQRPLDTLG TGPHCDPTSL 

       250        260        270        280        290        300 
TILHQDHVGG LEVWAEGRWR AIRPRPGALV VNVGDTFMAL SNARYRSCLH RAVVNSTAPR 

       310        320        330        340        350        360 
RSLAFFLCPE MDTVVRPPEE LVDDHHPRVY PDFTWRALLD FTQRHYRADM RLFQAFSDWL 

       370 
NHHRHLQPTI YS 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and characterization of a cDNA encoding gibberellin 20-oxidase from rice (Oryza sativa) seedlings."
Toyomasu T., Kawaide H., Sekimoto H., von Numers C., Phillips A.L., Hedden P., Kamiya Y.
Physiol. Plantarum 99:111-118(1997)
Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION, FUNCTION.
Strain: cv. Nipponbare.
[2]"Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species."
The rice chromosome 3 sequencing consortium
Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S. expand/collapse author list , Johri S., Reardon M., Webb K., Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T., Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R., Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J., Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S., Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M., Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M., Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L., O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R., Jin W., Lee H.R., Jiang J., Jackson S.
Genome Res. 15:1284-1291(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[3]"A mutant gibberellin-synthesis gene in rice."
Sasaki A., Ashikari M., Ueguchi-Tanaka M., Itoh H., Nishimura A., Swapan D., Ishiyama K., Saito T., Kobayashi M., Khush G.S., Kitano H., Matsuoka M.
Nature 416:701-702(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U50333 mRNA. Translation: AAB48239.1.
AC096690 Genomic DNA. Translation: AAP21386.1. Different initiation.
PIRT04337.

3D structure databases

ProteinModelPortalP93771.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

GrameneP93771.

Phylogenomic databases

eggNOGCOG3491.
HOGENOMHOG000276735.

Family and domain databases

Gene3D2.60.120.330. 1 hit.
InterProIPR026992. DIOX_N.
IPR027443. IPNS-like.
IPR002283. Isopenicillin-N_synthase.
IPR005123. Oxoglu/Fe-dep_dioxygenase.
[Graphical view]
PfamPF03171. 2OG-FeII_Oxy. 1 hit.
PF14226. DIOX_N. 1 hit.
[Graphical view]
PRINTSPR00682. IPNSYNTHASE.
PROSITEPS51471. FE2OG_OXY. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGAOX1_ORYSJ
AccessionPrimary (citable) accession number: P93771
Secondary accession number(s): Q84M83
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: March 1, 2005
Last modified: April 16, 2014
This is version 74 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations