Reviewed,
UniProtKB/Swiss-Prot P93771 (GAOX1_ORYSJ)
Last modified
January 19, 2010.
Version 51.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Gibberellin 20 oxidase 1 EC=1.14.11.- Alternative name(s): Gibberellin C-20 oxidase 1 GA 20-oxidase 1 Os20ox | ||||||||
| Gene names |
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| Organism | Oryza sativa subsp. japonica (Rice) | ||||||||
| Taxonomic identifier | 39947 [NCBI] | ||||||||
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza |
Protein attributes
| Sequence length | 372 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Function | Key oxidase enzyme in the biosynthesis of gibberellin that catalyzes the conversion of GA12 and GA53 to GA9 and GA20 respectively, via a three-step oxidation at C-20 of the GA skeleton. Ref.1 |
| Cofactor | Iron. Ascorbate. 2-oxoglutarate. |
| Tissue specificity | Preferentially expressed in reproductive organs. Ref.3 |
| Induction | Negatively controlled by the level of physiologically active gibberellin. Ref.1 |
| Sequence similarities | Belongs to the iron/ascorbate-dependent oxidoreductase family. GA20OX subfamily. Contains 1 Fe2OG dioxygenase domain. |
Ontologies
| Keywords | |
|---|---|
| Ligand | Iron Metal-binding |
| Molecular function | Oxidoreductase |
| Gene Ontology (GO) | |
| Biological process | gibberellin metabolic process Inferred by curator. Source: Gramene oxidation reductionInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW oxidoreductase activityInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 372 | 372 | Gibberellin 20 oxidase 1 | PRO_0000219517 | |||||
Regions | |||||||||
| Domain | 209 – 309 | 101 | Fe2OG dioxygenase | ||||||
Sites | |||||||||
| Active site | 300 | 1 | Potential | ||||||
| Metal binding | 234 | 1 | Iron By similarity | ||||||
| Metal binding | 236 | 1 | Iron By similarity | ||||||
| Metal binding | 290 | 1 | Iron By similarity | ||||||
Experimental info | |||||||||
| Sequence conflict | 107 – 116 | 10 | AQRRAGESCG → RSGARGRTA in AAB48239. Ref.1 | ||||||
| Sequence conflict | 352 | 1 | L → F Ref.1 | ||||||
Sequences
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References
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | U50333 mRNA. Translation: AAB48239.1. AC096690 Genomic DNA. Translation: AAP21386.1. Different initiation. |
| PIR | T04337. |
3D structure databases | |
| SMR | P93771. Positions 17-340. |
| ModBase | Search... |
Organism-specific databases | |
| Gramene | P93771. |
Family and domain databases | |
| InterPro | IPR002283. Isopenicillin-N_synthase. IPR005123. Oxoglutarate/Fe-dep_oxygenase. [Graphical view] |
| Pfam | PF03171. 2OG-FeII_Oxy. 1 hit. [Graphical view] |
| PRINTS | PR00682. IPNSYNTHASE. |
| PROSITE | PS51471. FE2OG_OXY. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | GAOX1_ORYSJ | ||||||||
| Accession | Primary (citable) accession number: P93771 Secondary accession number(s): Q84M83 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | PPAP (Plant Proteome Annotation Project) | ||||||||
Relevant documents
| Oryza sativa (rice) Index of Oryza sativa entries and their corresponding gene designations |
| SIMILARITY comments Index of protein domains and families |

Clusters with


