Reviewed, UniProtKB/Swiss-Prot P93771 (GAOX1_ORYSJ)

Names and origin

Protein names

Recommended name:
    Gibberellin 20 oxidase 1
    EC=1.14.11.-
Alternative name(s):
    Gibberellin C-20 oxidase 1
    GA 20-oxidase 1
    Os20ox

Gene names

Name:	20ox1
Synonyms:	GA20ox-1
Ordered Locus Names:	Os03g0856700, LOC_Os03g63970
ORF Names:	OSJNBa0059G06.22

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	372 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Key oxidase enzyme in the biosynthesis of gibberellin that catalyzes the conversion of GA12 and GA53 to GA9 and GA20 respectively, via a three-step oxidation at C-20 of the GA skeleton. Ref.1
Cofactor	Iron. Ascorbate. 2-oxoglutarate.
Tissue specificity	Preferentially expressed in reproductive organs. Ref.3
Induction	Negatively controlled by the level of physiologically active gibberellin. Ref.1
Sequence similarities	Belongs to the iron/ascorbate-dependent oxidoreductase family. GA20OX subfamily. Contains 1 Fe2OG dioxygenase domain.

Ontologies

Keywords
Ligand	Iron Metal-binding
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	gibberellin metabolic process Inferred by curator. Source: Gramene oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW oxidoreductase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 372	372	Gibberellin 20 oxidase 1		PRO_0000219517
Regions
	Domain	209 – 309	101	Fe2OG dioxygenase
Sites
	Active site	300	1	Potential
	Metal binding	234	1	Iron By similarity
	Metal binding	236	1	Iron By similarity
	Metal binding	290	1	Iron By similarity
Experimental info
	Sequence conflict	107 – 116	10	AQRRAGESCG → RSGARGRTA in AAB48239. Ref.1
	Sequence conflict	352	1	L → F Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P93771-1 [UniParc]. Last modified March 1, 2005. Version 2. Checksum: 6BC53284017F9C27	FASTA	372	42,302
	10 20 30 40 50 60 MSMVVQQEQE VVFDAAVLSG QTEIPSQFIW PAEESPGSVA VEELEVALID VGAGAERSSV 70 80 90 100 110 120 VRQVGEACER HGFFLVVNHG IEAALLEEAH RCMDAFFTLP LGEKQRAQRR AGESCGYASS 130 140 150 160 170 180 FTGRFASKLP WKETLSFRYS SAGDEEGEEG VGEYLVRKLG AEHGRRLGEV YSRYCHEMSR 190 200 210 220 230 240 LSLELMEVLG ESLGIVGDRR HYFRRFFQRN DSIMRLNYYP ACQRPLDTLG TGPHCDPTSL 250 260 270 280 290 300 TILHQDHVGG LEVWAEGRWR AIRPRPGALV VNVGDTFMAL SNARYRSCLH RAVVNSTAPR 310 320 330 340 350 360 RSLAFFLCPE MDTVVRPPEE LVDDHHPRVY PDFTWRALLD FTQRHYRADM RLFQAFSDWL 370 NHHRHLQPTI YS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of a cDNA encoding gibberellin 20-oxidase from rice (Oryza sativa) seedlings." Toyomasu T., Kawaide H., Sekimoto H., von Numers C., Phillips A.L., Hedden P., Kamiya Y. Physiol. Plantarum 99:111-118(1997) Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION, FUNCTION. Strain: cv. Nipponbare.
[2]	"Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species." The rice chromosome 3 sequencing consortium Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S. , Johri S., Reardon M., Webb K., Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T., Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R., Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J., Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S., Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M., Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M., Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L., O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R., Jin W., Lee H.R., Jiang J., Jackson S. Genome Res. 15:1284-1291(2005) [PubMed: 16109971] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"A mutant gibberellin-synthesis gene in rice." Sasaki A., Ashikari M., Ueguchi-Tanaka M., Itoh H., Nishimura A., Swapan D., Ishiyama K., Saito T., Kobayashi M., Khush G.S., Kitano H., Matsuoka M. Nature 416:701-702(2002) [PubMed: 11961544] [Abstract] Cited for: TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U50333 mRNA. Translation: AAB48239.1. AC096690 Genomic DNA. Translation: AAP21386.1. Different initiation.
PIR	T04337.
3D structure databases
SMR	P93771. Positions 17-340.
ModBase	Search...
Organism-specific databases
Gramene	P93771.
Family and domain databases
InterPro	IPR002283. Isopenicillin-N_synthase. IPR005123. Oxoglutarate/Fe-dep_oxygenase. [Graphical view]
Pfam	PF03171. 2OG-FeII_Oxy. 1 hit. [Graphical view]
PRINTS	PR00682. IPNSYNTHASE.
PROSITE	PS51471. FE2OG_OXY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GAOX1_ORYSJ

Accession

Primary (citable) accession number: P93771
Secondary accession number(s): Q84M83

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2005
Last sequence update:	March 1, 2005
Last modified:	January 19, 2010
This is version 51 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)