Gibberellin 20 oxidase 1 - Oryza sativa subsp. japonica (Rice)

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P93771 (GAOX1_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 70. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Gibberellin 20 oxidase 1
EC=1.14.11.-

Alternative name(s):
GA 20-oxidase 1
Gibberellin C-20 oxidase 1
Os20ox

Gene names

Name:	20ox1
Synonyms:	GA20ox-1
Ordered Locus Names:	Os03g0856700, LOC_Os03g63970
ORF Names:	OSJNBa0059G06.22

Organism

Oryza sativa subsp. japonica (Rice) [Reference proteome]

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza ›

Protein attributes

Sequence length	372 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Key oxidase enzyme in the biosynthesis of gibberellin that catalyzes the conversion of GA12 and GA53 to GA9 and GA20 respectively, via a three-step oxidation at C-20 of the GA skeleton. Ref.1
Cofactor	Iron. Ascorbate. 2-oxoglutarate.
Tissue specificity	Preferentially expressed in reproductive organs. Ref.3
Induction	Negatively controlled by the level of physiologically active gibberellin. Ref.1
Sequence similarities	Belongs to the iron/ascorbate-dependent oxidoreductase family. GA20OX subfamily. Contains 1 Fe2OG dioxygenase domain.
Sequence caution	The sequence AAP21386.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Ligand	Iron Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	flower development Inferred from electronic annotation. Source: EnsemblPlants/Gramene gibberellin biosynthetic process Inferred from electronic annotation. Source: EnsemblPlants/Gramene gibberellin metabolic process Inferred by curator PubMed 15075394. Source: Gramene leaf development Inferred from electronic annotation. Source: EnsemblPlants/Gramene response to gibberellin stimulus Inferred from electronic annotation. Source: EnsemblPlants/Gramene response to karrikin Inferred from electronic annotation. Source: EnsemblPlants/Gramene response to red or far red light Inferred from electronic annotation. Source: EnsemblPlants/Gramene short-day photoperiodism, flowering Inferred from electronic annotation. Source: EnsemblPlants/Gramene unidimensional cell growth Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Molecular_function	gibberellin 20-oxidase activity Inferred from electronic annotation. Source: EnsemblPlants/Gramene iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 372

372

Gibberellin 20 oxidase 1

PRO_0000219517

Regions

Domain

209 – 309

101

Fe2OG dioxygenase

Sites

Active site

300

Potential

Metal binding

234

Iron By similarity

Metal binding

236

Iron By similarity

Metal binding

290

Iron By similarity

Experimental info

Sequence conflict

107 – 116

AQRRAGESCG → RSGARGRTA in AAB48239. Ref.1

Sequence conflict

352

L → F Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

P93771 [UniParc].

Last modified March 1, 2005. Version 2.
Checksum: 6BC53284017F9C27
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FASTA

372

42,302

        10         20         30         40         50         60 
MSMVVQQEQE VVFDAAVLSG QTEIPSQFIW PAEESPGSVA VEELEVALID VGAGAERSSV 

        70         80         90        100        110        120 
VRQVGEACER HGFFLVVNHG IEAALLEEAH RCMDAFFTLP LGEKQRAQRR AGESCGYASS 

       130        140        150        160        170        180 
FTGRFASKLP WKETLSFRYS SAGDEEGEEG VGEYLVRKLG AEHGRRLGEV YSRYCHEMSR 

       190        200        210        220        230        240 
LSLELMEVLG ESLGIVGDRR HYFRRFFQRN DSIMRLNYYP ACQRPLDTLG TGPHCDPTSL 

       250        260        270        280        290        300 
TILHQDHVGG LEVWAEGRWR AIRPRPGALV VNVGDTFMAL SNARYRSCLH RAVVNSTAPR 

       310        320        330        340        350        360 
RSLAFFLCPE MDTVVRPPEE LVDDHHPRVY PDFTWRALLD FTQRHYRADM RLFQAFSDWL 

       370 
NHHRHLQPTI YS

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of a cDNA encoding gibberellin 20-oxidase from rice (Oryza sativa) seedlings." Toyomasu T., Kawaide H., Sekimoto H., von Numers C., Phillips A.L., Hedden P., Kamiya Y. Physiol. Plantarum 99:111-118(1997) Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION, FUNCTION. Strain: cv. Nipponbare.
[2]	"Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species." The rice chromosome 3 sequencing consortium Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S. Jackson S. Genome Res. 15:1284-1291(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"A mutant gibberellin-synthesis gene in rice." Sasaki A., Ashikari M., Ueguchi-Tanaka M., Itoh H., Nishimura A., Swapan D., Ishiyama K., Saito T., Kobayashi M., Khush G.S., Kitano H., Matsuoka M. Nature 416:701-702(2002) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U50333 mRNA. Translation: AAB48239.1. AC096690 Genomic DNA. Translation: AAP21386.1. Different initiation.
PIR	T04337.
3D structure databases
ProteinModelPortal	P93771.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
KEGG	dosa:Os03t0856700-01.
Organism-specific databases
Gramene	P93771.
Phylogenomic databases
eggNOG	COG3491.
HOGENOM	HOG000276735.
Gene expression databases
ArrayExpress	P93771.
Family and domain databases
InterPro	IPR026992. DIOX_N. IPR002283. Isopenicillin-N_synthase. IPR005123. Oxoglu/Fe-dep_dioxygenase. [Graphical view]
Pfam	PF03171. 2OG-FeII_Oxy. 1 hit. PF14226. DIOX_N. 1 hit. [Graphical view]
PRINTS	PR00682. IPNSYNTHASE.
PROSITE	PS51471. FE2OG_OXY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GAOX1_ORYSJ

Accession

Primary (citable) accession number: P93771
Secondary accession number(s): Q84M83

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2005
Last sequence update:	March 1, 2005
Last modified:	April 3, 2013
This is version 70 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents