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P93771

- GAOX1_ORYSJ

UniProt

P93771 - GAOX1_ORYSJ

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Protein

Gibberellin 20 oxidase 1

Gene
20ox1, GA20ox-1, Os03g0856700, LOC_Os03g63970, OSJNBa0059G06.22
Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Key oxidase enzyme in the biosynthesis of gibberellin that catalyzes the conversion of GA12 and GA53 to GA9 and GA20 respectively, via a three-step oxidation at C-20 of the GA skeleton.1 Publication

Cofactori

Iron.
Ascorbate.
2-oxoglutarate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi234 – 2341Iron By similarity
Metal bindingi236 – 2361Iron By similarity
Metal bindingi290 – 2901Iron By similarity
Active sitei300 – 3001 Reviewed prediction

GO - Molecular functioni

  1. iron ion binding Source: InterPro
  2. oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors Source: InterPro

GO - Biological processi

  1. gibberellin metabolic process Source: Gramene
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Gibberellin 20 oxidase 1 (EC:1.14.11.-)
Alternative name(s):
GA 20-oxidase 1
Gibberellin C-20 oxidase 1
Os20ox
Gene namesi
Name:20ox1
Synonyms:GA20ox-1
Ordered Locus Names:Os03g0856700, LOC_Os03g63970
ORF Names:OSJNBa0059G06.22
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
ProteomesiUP000000763: Chromosome 3

Organism-specific databases

GrameneiP93771.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 372372Gibberellin 20 oxidase 1PRO_0000219517Add
BLAST

Expressioni

Tissue specificityi

Preferentially expressed in reproductive organs.1 Publication

Inductioni

Negatively controlled by the level of physiologically active gibberellin.1 Publication

Structurei

3D structure databases

ProteinModelPortaliP93771.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini209 – 309101Fe2OG dioxygenaseAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG3491.
HOGENOMiHOG000276735.

Family and domain databases

Gene3Di2.60.120.330. 1 hit.
InterProiIPR026992. DIOX_N.
IPR027443. IPNS-like.
IPR002283. Isopenicillin-N_synthase.
IPR005123. Oxoglu/Fe-dep_dioxygenase.
[Graphical view]
PfamiPF03171. 2OG-FeII_Oxy. 1 hit.
PF14226. DIOX_N. 1 hit.
[Graphical view]
PRINTSiPR00682. IPNSYNTHASE.
PROSITEiPS51471. FE2OG_OXY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P93771-1 [UniParc]FASTAAdd to Basket

« Hide

MSMVVQQEQE VVFDAAVLSG QTEIPSQFIW PAEESPGSVA VEELEVALID    50
VGAGAERSSV VRQVGEACER HGFFLVVNHG IEAALLEEAH RCMDAFFTLP 100
LGEKQRAQRR AGESCGYASS FTGRFASKLP WKETLSFRYS SAGDEEGEEG 150
VGEYLVRKLG AEHGRRLGEV YSRYCHEMSR LSLELMEVLG ESLGIVGDRR 200
HYFRRFFQRN DSIMRLNYYP ACQRPLDTLG TGPHCDPTSL TILHQDHVGG 250
LEVWAEGRWR AIRPRPGALV VNVGDTFMAL SNARYRSCLH RAVVNSTAPR 300
RSLAFFLCPE MDTVVRPPEE LVDDHHPRVY PDFTWRALLD FTQRHYRADM 350
RLFQAFSDWL NHHRHLQPTI YS 372
Length:372
Mass (Da):42,302
Last modified:March 1, 2005 - v2
Checksum:i6BC53284017F9C27
GO

Sequence cautioni

The sequence AAP21386.1 differs from that shown. Reason: Erroneous initiation.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti107 – 11610AQRRAGESCG → RSGARGRTA in AAB48239. 1 Publication
Sequence conflicti352 – 3521L → F1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U50333 mRNA. Translation: AAB48239.1.
AC096690 Genomic DNA. Translation: AAP21386.1. Different initiation.
PIRiT04337.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U50333 mRNA. Translation: AAB48239.1 .
AC096690 Genomic DNA. Translation: AAP21386.1 . Different initiation.
PIRi T04337.

3D structure databases

ProteinModelPortali P93771.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Organism-specific databases

Gramenei P93771.

Phylogenomic databases

eggNOGi COG3491.
HOGENOMi HOG000276735.

Family and domain databases

Gene3Di 2.60.120.330. 1 hit.
InterProi IPR026992. DIOX_N.
IPR027443. IPNS-like.
IPR002283. Isopenicillin-N_synthase.
IPR005123. Oxoglu/Fe-dep_dioxygenase.
[Graphical view ]
Pfami PF03171. 2OG-FeII_Oxy. 1 hit.
PF14226. DIOX_N. 1 hit.
[Graphical view ]
PRINTSi PR00682. IPNSYNTHASE.
PROSITEi PS51471. FE2OG_OXY. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of a cDNA encoding gibberellin 20-oxidase from rice (Oryza sativa) seedlings."
    Toyomasu T., Kawaide H., Sekimoto H., von Numers C., Phillips A.L., Hedden P., Kamiya Y.
    Physiol. Plantarum 99:111-118(1997)
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION, FUNCTION.
    Strain: cv. Nipponbare.
  2. "Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species."
    The rice chromosome 3 sequencing consortium
    Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S.
    , Johri S., Reardon M., Webb K., Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T., Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R., Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J., Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S., Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M., Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M., Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L., O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R., Jin W., Lee H.R., Jiang J., Jackson S.
    Genome Res. 15:1284-1291(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  3. Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiGAOX1_ORYSJ
AccessioniPrimary (citable) accession number: P93771
Secondary accession number(s): Q84M83
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: March 1, 2005
Last modified: April 16, 2014
This is version 74 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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