Reviewed, UniProtKB/Swiss-Prot P93761 (INV1_CAPAN)

Names and origin

Protein names	Recommended name:     Acid beta-fructofuranosidase AIV-18     EC=3.2.1.26 Alternative name(s):     Acid sucrose hydrolase     Acid invertase
Organism	Capsicum annuum (Bell pepper)
Taxonomic identifier	4072 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Capsiceae › Capsicum

Protein attributes

Sequence length	640 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides.
Subcellular location	Membrane; Single-pass membrane protein Potential.
Sequence similarities	Belongs to the glycosyl hydrolase 32 family.

Ontologies

Keywords
Cellular component	Membrane
Domain	Transmembrane
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	beta-fructofuranosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 640	640	Acid beta-fructofuranosidase AIV-18		PRO_0000169866
Regions
	Transmembrane	34 – 54	21	Potential
Sites
	Active site	126	1	By similarity
Amino acid modifications
	Glycosylation	152	1	N-linked (GlcNAc...) Potential
	Glycosylation	219	1	N-linked (GlcNAc...) Potential
	Glycosylation	491	1	N-linked (GlcNAc...) Potential
	Glycosylation	612	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P93761-1 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: D3C628B7A7E6870B	FASTA	640	70,621
	10 20 30 40 50 60 MAIHPSSYDP ETSTTHYTFL PGQPDSGHRK SIKVVSVILL SSFFLLYLAA FVILNNQPPN 70 80 90 100 110 120 LQNKSPSASE TLTPATPSRG VSQGVSEKTF KDVSGTSQVS YTWSNAMLNW QRTAYHFQPQ 130 140 150 160 170 180 KNWMNDPNGP LYHKGWYHLF YQYNPDSAIW GNITWGHAVS TDLIHWLYLP FAMVPDQWYD 190 200 210 220 230 240 INGVWTGSAT ILPDGLIMML YTGDTDDYVQ VQNLAYPANL SDPLLLDWVK YQGNPVLVPP 250 260 270 280 290 300 PGIGVKDFRD PTTAWTGPQN GQWLLTIGSK VGKTGIALVY ETSNFKLLDG VLHAVPGTGM 310 320 330 340 350 360 WECVDFYPVS TLDANGLDTS YNGPGIKHVL KASLDDNKQD HYVIGTYDPV KNKFSPDNPD 370 380 390 400 410 420 LDCGIGLRLD YGRYYASKTF YDPKKQRRVL WGWIGETDSE SADLQKGWAS VQSIPRTVLF 430 440 450 460 470 480 DKKTGTHLLQ WPVAEIESLR SGDPKVKEVN LQPGSIELLH VDSAAQFDIE ASFEVDRVTL 490 500 510 520 530 540 EGIIEADVGY NCSTSGGAAS RGILGPFGVV VIADQTLSEL TPVYFYISRG ADGRAEAHFC 550 560 570 580 590 600 ADQTRSSEAP GVAKQVYGSS VPVLDGEKHR MRLLVDHSIV ESFAQGGRTV ITSRIYPTKA 610 620 630 640 VNGAARLFVF NNATGAIVTA SLKIWSLESA DIRSFPLQKL

References

[1]	"Isolation and characterization of acid invertase cDNA clone in Hot pepper (Capsicum annuum L.) fruits." Choi D., Lee K.-W., Kim S. J. Plant Biol. 40:298-303(1997) Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Fruit.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U87849 mRNA. Translation: AAB48484.1.
PIR	T09534.
3D structure databases
SMR	P93761. Positions 112-632.
ModBase	Search...
Protein family/group databases
CAZy	GH32. Glycoside Hydrolase Family 32.
Enzyme and pathway databases
BRENDA	3.2.1.26. 1261.
Family and domain databases
InterPro	IPR008985. ConA-like_lec_gl. IPR001362. Glyco_hydro_32. IPR018053. Glyco_hydro_32_AS. IPR013189. Glyco_hydro_32_C. IPR013148. Glyco_hydro_32_N. [Graphical view]
Pfam	PF08244. Glyco_hydro_32C. 1 hit. PF00251. Glyco_hydro_32N. 1 hit. [Graphical view]
SMART	SM00640. Glyco_32. 1 hit. [Graphical view]
PROSITE	PS00609. GLYCOSYL_HYDROL_F32. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

INV1_CAPAN

Accession

Primary (citable) accession number: P93761

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	May 1, 1997
Last modified:	January 19, 2010
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)