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Protein

Calcium-dependent protein kinase 14

Gene

CPK14

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

May play a role in signal transduction pathways that involve calcium as a second messenger.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by calcium. Autophosphorylation may play an important role in the regulation of the kinase activity (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei83 – 831ATPPROSITE-ProRule annotation
Active sitei178 – 1781Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi60 – 689ATPPROSITE-ProRule annotation
Calcium bindingi368 – 379121PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi404 – 415122PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi440 – 451123PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi476 – 487124PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Calcium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT2G41860-MONOMER.
ARA:GQT-1912-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-dependent protein kinase 14 (EC:2.7.11.1)
Gene namesi
Name:CPK14
Ordered Locus Names:At2g41860
ORF Names:T11A7.4, T6D20.24
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G41860.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: TAIR
  • nucleus Source: GO_Central
  • plasma membrane Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedSequence analysis
Chaini2 – 530529Calcium-dependent protein kinase 14PRO_0000363336Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycineSequence analysis

Keywords - PTMi

Lipoprotein, Myristate

Proteomic databases

PaxDbiP93759.
PRIDEiP93759.

Expressioni

Gene expression databases

ExpressionAtlasiP93759. baseline and differential.
GenevisibleiP93759. AT.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

STRINGi3702.AT2G41860.1.

Structurei

3D structure databases

ProteinModelPortaliP93759.
SMRiP93759. Positions 52-493.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini54 – 312259Protein kinasePROSITE-ProRule annotationAdd
BLAST
Domaini355 – 39036EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini391 – 42636EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini427 – 46236EF-hand 3PROSITE-ProRule annotationAdd
BLAST
Domaini463 – 49836EF-hand 4PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni318 – 34831Autoinhibitory domainBy similarityAdd
BLAST

Domaini

There is 3 contiguous domains conserved in the CDPK subfamily: a kinase domain, an autoinhibitory (junction) domain and a calmodulin-like domain. The autoinhibitory domain (318-348) inactivates kinase activity under calcium-free conditions (By similarity).By similarity

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.PROSITE-ProRule annotation
Contains 4 EF-hand domains.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0032. Eukaryota.
ENOG410XRMJ. LUCA.
HOGENOMiHOG000233030.
InParanoidiP93759.
KOiK13412.
OMAiETYEDDK.
PhylomeDBiP93759.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF13499. EF-hand_7. 2 hits.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 4 hits.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00018. EF_HAND_1. 3 hits.
PS50222. EF_HAND_2. 4 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: P93759-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGNCCGTAGS LIQDKQKKGF KLPNPFSNEY GNHHDGLKLI VLKEPTGHEI
60 70 80 90 100
KQKYKLGREL GRGEFGVTYL CTEIETGEIF ACKSILKKKL KTSIDIEDVK
110 120 130 140 150
REVEIMRQMP EHPNIVTLKE TYEDDKAVHL VMELCEGGEL FDRIVARGHY
160 170 180 190 200
TERAAASVIK TIIEVVQMCH KHGVMHRDLK PENFLFANKK ETASLKAIDF
210 220 230 240 250
GLSVFFKPGE RFNEIVGSPY YMAPEVLRRS YGQEIDIWSA GVILYILLCG
260 270 280 290 300
VPPFWAETEH GVAKAILKSV IDFKRDPWPK VSDNAKDLIK KMLHPDPRRR
310 320 330 340 350
LTAQQVLDHP WIQNGKNASN VSLGETVRAR LKQFSVMNKL KKRALRVIAE
360 370 380 390 400
HLSVEETSCI KERFQVMDTS NRGKITITEL GIGLQKLGIV VPQDDIQILM
410 420 430 440 450
DAGDVDKDGY LDVNEFVAIS VHIRKLGNDE HLKKAFTFFD KNKSGYIEIE
460 470 480 490 500
ELRDALADDV DTTSEEVVEA IILDVDTNKD GKISYDEFAT MMKTGTDWRK
510 520 530
ASRQYSRDLF KCLSLKLMQD GSLQSNGDTK
Length:530
Mass (Da):60,054
Last modified:May 1, 1997 - v1
Checksum:iB5E4E565E2018DAB
GO

Sequence cautioni

The sequence ABK28533.1 differs from that shown. Reason: Erroneous termination at position 531. Translated as stop.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC002339 Genomic DNA. Translation: AAM14824.1.
U90439 Genomic DNA. Translation: AAB63555.1.
CP002685 Genomic DNA. Translation: AEC10041.1.
DQ446617 mRNA. Translation: ABE65901.1.
DQ653054 mRNA. Translation: ABK28533.1. Sequence problems.
PIRiA84847.
RefSeqiNP_181717.3. NM_129750.3. [P93759-1]
UniGeneiAt.50130.

Genome annotation databases

EnsemblPlantsiAT2G41860.1; AT2G41860.1; AT2G41860. [P93759-1]
GeneIDi818786.
KEGGiath:AT2G41860.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC002339 Genomic DNA. Translation: AAM14824.1.
U90439 Genomic DNA. Translation: AAB63555.1.
CP002685 Genomic DNA. Translation: AEC10041.1.
DQ446617 mRNA. Translation: ABE65901.1.
DQ653054 mRNA. Translation: ABK28533.1. Sequence problems.
PIRiA84847.
RefSeqiNP_181717.3. NM_129750.3. [P93759-1]
UniGeneiAt.50130.

3D structure databases

ProteinModelPortaliP93759.
SMRiP93759. Positions 52-493.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT2G41860.1.

Proteomic databases

PaxDbiP93759.
PRIDEiP93759.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G41860.1; AT2G41860.1; AT2G41860. [P93759-1]
GeneIDi818786.
KEGGiath:AT2G41860.

Organism-specific databases

TAIRiAT2G41860.

Phylogenomic databases

eggNOGiKOG0032. Eukaryota.
ENOG410XRMJ. LUCA.
HOGENOMiHOG000233030.
InParanoidiP93759.
KOiK13412.
OMAiETYEDDK.
PhylomeDBiP93759.

Enzyme and pathway databases

BioCyciARA:AT2G41860-MONOMER.
ARA:GQT-1912-MONOMER.

Miscellaneous databases

PROiP93759.

Gene expression databases

ExpressionAtlasiP93759. baseline and differential.
GenevisibleiP93759. AT.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF13499. EF-hand_7. 2 hits.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 4 hits.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00018. EF_HAND_1. 3 hits.
PS50222. EF_HAND_2. 4 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Simultaneous high-throughput recombinational cloning of open reading frames in closed and open configurations."
    Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P.
    Plant Biotechnol. J. 4:317-324(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "The CDPK superfamily of protein kinases."
    Harmon A.C., Gribskov M., Gubrium E., Harper J.F.
    New Phytol. 151:175-183(2001)
    Cited for: GENE FAMILY, NOMENCLATURE.
  5. "Calcium signaling through protein kinases. The Arabidopsis calcium-dependent protein kinase gene family."
    Cheng S.-H., Willmann M.R., Chen H.-C., Sheen J.
    Plant Physiol. 129:469-485(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  6. Cited for: GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiCDPKE_ARATH
AccessioniPrimary (citable) accession number: P93759
Secondary accession number(s): A0MET2, Q3EBI3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: May 1, 1997
Last modified: June 8, 2016
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.