ID   MT4A_ARATH              Reviewed;          84 AA.
AC   P93746; F4ILY8; Q42258;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 2.
DT   24-JAN-2024, entry version 132.
DE   RecName: Full=Metallothionein-like protein 4A;
DE            Short=MT-4A;
DE   AltName: Full=EC protein homolog 1;
GN   Name=MT4A; OrderedLocusNames=At2g42000; ORFNames=T6D20.11;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10617197; DOI=10.1038/45471;
RA   Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA   Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA   Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA   Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA   Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA   Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA   Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT   "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL   Nature 402:761-768(1999).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 3-84.
RC   STRAIN=cv. Columbia; TISSUE=Dry seed;
RX   PubMed=8580968; DOI=10.1046/j.1365-313x.1996.09010101.x;
RA   Cooke R., Raynal M., Laudie M., Grellet F., Delseny M., Morris P.-C.,
RA   Guerrier D., Giraudat J., Quigley F., Clabault G., Li Y.-F., Mache R.,
RA   Krivitzky M., Gy I.J.-J., Kreis M., Lecharny A., Parmentier Y., Marbach J.,
RA   Fleck J., Clement B., Philipps G., Herve C., Bardet C., Tremousaygue D.,
RA   Lescure B., Lacomme C., Roby D., Jourjon M.-F., Chabrier P.,
RA   Charpenteau J.-L., Desprez T., Amselem J., Chiapello H., Hoefte H.;
RT   "Further progress towards a catalogue of all Arabidopsis genes: analysis of
RT   a set of 5000 non-redundant ESTs.";
RL   Plant J. 9:101-124(1996).
RN   [4]
RP   FUNCTION.
RX   PubMed=18287486; DOI=10.1104/pp.108.115782;
RA   Guo W.J., Meetam M., Goldsbrough P.B.;
RT   "Examining the specific contributions of individual Arabidopsis
RT   metallothioneins to copper distribution and metal tolerance.";
RL   Plant Physiol. 146:1697-1706(2008).
RN   [5]
RP   FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE,
RP   AND INDUCTION.
RX   PubMed=22014117; DOI=10.1111/j.1365-3040.2011.02450.x;
RA   Ren Y., Liu Y., Chen H., Li G., Zhang X., Zhao J.;
RT   "Type 4 metallothionein genes are involved in regulating Zn ion
RT   accumulation in late embryo and in controlling early seedling growth in
RT   Arabidopsis.";
RL   Plant Cell Environ. 35:770-789(2012).
CC   -!- FUNCTION: Metallothioneins have a high content of cysteine residues
CC       that bind various heavy metals (Probable). Functions as a metal
CC       chelator of copper (Cu) and zinc (Zn) (PubMed:18287486). Plays a role
CC       in storing and distributing Zn ion in seed (PubMed:22014117).
CC       {ECO:0000269|PubMed:18287486, ECO:0000269|PubMed:22014117,
CC       ECO:0000305}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:22014117}. Nucleus
CC       {ECO:0000269|PubMed:22014117}. Cell membrane
CC       {ECO:0000269|PubMed:22014117}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=1;
CC         Comment=A number of isoforms are produced. According to EST
CC         sequences.;
CC       Name=1;
CC         IsoId=P93746-1; Sequence=Displayed;
CC   -!- TISSUE SPECIFICITY: Expressed specifically in seeds.
CC       {ECO:0000269|PubMed:22014117}.
CC   -!- DEVELOPMENTAL STAGE: During embryo development, expressed from torpedo
CC       stage (6 days after pollination) to mature embryo.
CC       {ECO:0000269|PubMed:22014117}.
CC   -!- INDUCTION: By abscisic acid (ABA) and jasmonic acid. Down-regulated by
CC       gibberellin. {ECO:0000269|PubMed:22014117}.
CC   -!- MISCELLANEOUS: Plants silencing both MT4A and MT4B have reduced seed
CC       weight and reduced seedling growth after germination.
CC       {ECO:0000269|PubMed:22014117}.
CC   -!- SIMILARITY: Belongs to the metallothionein superfamily. Type 15 family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAB63543.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; U90439; AAB63543.1; ALT_INIT; Genomic_DNA.
DR   EMBL; CP002685; AEC10060.2; -; Genomic_DNA.
DR   EMBL; Z32602; CAA83560.1; -; mRNA.
DR   PIR; G84848; G84848.
DR   RefSeq; NP_181731.2; NM_129764.2. [P93746-1]
DR   AlphaFoldDB; P93746; -.
DR   SMR; P93746; -.
DR   STRING; 3702.P93746; -.
DR   PaxDb; 3702-AT2G42000-2; -.
DR   EnsemblPlants; AT2G42000.1; AT2G42000.1; AT2G42000. [P93746-1]
DR   GeneID; 818800; -.
DR   Gramene; AT2G42000.1; AT2G42000.1; AT2G42000. [P93746-1]
DR   KEGG; ath:AT2G42000; -.
DR   Araport; AT2G42000; -.
DR   TAIR; AT2G42000; ATMT4A.
DR   eggNOG; ENOG502S74E; Eukaryota.
DR   InParanoid; P93746; -.
DR   PhylomeDB; P93746; -.
DR   PRO; PR:P93746; -.
DR   Proteomes; UP000006548; Chromosome 2.
DR   ExpressionAtlas; P93746; baseline and differential.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   InterPro; IPR000316; Metallthion_15.
DR   PANTHER; PTHR48198; EC PROTEIN HOMOLOG; 1.
DR   PANTHER; PTHR48198:SF1; METALLOTHIONEIN-LIKE PROTEIN 4A-RELATED; 1.
DR   Pfam; PF02068; Metallothio_PEC; 1.
DR   PRINTS; PR00877; MTPLANTPEC.
DR   Genevisible; P93746; AT.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Cell membrane; Cytoplasm; Membrane; Metal-binding;
KW   Metal-thiolate cluster; Nucleus; Reference proteome; Zinc.
FT   CHAIN           1..84
FT                   /note="Metallothionein-like protein 4A"
FT                   /id="PRO_0000197423"
FT   REGION          1..26
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        68
FT                   /note="A -> D (in Ref. 3; CAA83560)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        83
FT                   /note="A -> D (in Ref. 3; CAA83560)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   84 AA;  8290 MW;  09D76F26CDF87676 CRC64;
     MADTGKGSSV AGCNDSCGCP SPCPGGNSCR CRMREASAGD QGHMVCPCGE HCGCNPCNCP
     KTQTQTSAKG CTCGEGCTCA SCAT
//