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Protein

Metallothionein-like protein 4A

Gene

MT4A

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Metallothioneins have a high content of cysteine residues that bind various heavy metals (Probable). Functions as metal chelator of copper (Cu) and zinc (Zn) (PubMed:18287486). Plays a role in storing and distributing Zn ion in seed (PubMed:22014117).Curated2 Publications

GO - Molecular functioni

Complete GO annotation...

Keywords - Ligandi

Metal-binding, Metal-thiolate cluster, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Metallothionein-like protein 4A
Short name:
MT-4A
Alternative name(s):
EC protein homolog 1
Gene namesi
Name:MT4A
Ordered Locus Names:At2g42000
ORF Names:T6D20.11
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G42000.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Nucleus 1 Publication
  • Cell membrane 1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 8484Metallothionein-like protein 4APRO_0000197423Add
BLAST

Proteomic databases

PaxDbiP93746.

Expressioni

Tissue specificityi

Expressed specifically in seeds.1 Publication

Developmental stagei

During embryo development, expressed from torpedo stage (6 days after pollination) to mature embryo.1 Publication

Inductioni

By abscisic acid (ABA) and jasmonic acid. Down-regulated by gibberellin.1 Publication

Gene expression databases

ExpressionAtlasiP93746. differential.
GenevisibleiP93746. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G42000.2.

Structurei

3D structure databases

ProteinModelPortaliP93746.
SMRiP93746. Positions 11-39.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG410J62V. Eukaryota.
ENOG410Z4N5. LUCA.
HOGENOMiHOG000243560.
InParanoidiP93746.
PhylomeDBiP93746.

Family and domain databases

InterProiIPR000316. Metallthion_15.
[Graphical view]
PfamiPF02068. Metallothio_PEC. 1 hit.
[Graphical view]
PRINTSiPR00877. MTPLANTPEC.

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: P93746-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MADTGKGSSV AGCNDSCGCP SPCPGGNSCR CRMREASAGD QGHMVCPCGE
60 70 80
HCGCNPCNCP KTQTQTSAKG CTCGEGCTCA SCAT
Length:84
Mass (Da):8,290
Last modified:November 1, 1997 - v2
Checksum:i09D76F26CDF87676
GO

Sequence cautioni

The sequence AAB63543.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence AEC10060.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti68 – 681A → D in CAA83560 (PubMed:8580968).Curated
Sequence conflicti83 – 831A → D in CAA83560 (PubMed:8580968).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U90439 Genomic DNA. Translation: AAB63543.1. Different initiation.
CP002685 Genomic DNA. Translation: AEC10060.1. Different initiation.
Z32602 mRNA. Translation: CAA83560.1.
PIRiG84848.
RefSeqiNP_181731.1. NM_129764.1.
UniGeneiAt.42759.

Genome annotation databases

EnsemblPlantsiAT2G42000.1; AT2G42000.1; AT2G42000.
GeneIDi818800.
GrameneiAT2G42000.1; AT2G42000.1; AT2G42000.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U90439 Genomic DNA. Translation: AAB63543.1. Different initiation.
CP002685 Genomic DNA. Translation: AEC10060.1. Different initiation.
Z32602 mRNA. Translation: CAA83560.1.
PIRiG84848.
RefSeqiNP_181731.1. NM_129764.1.
UniGeneiAt.42759.

3D structure databases

ProteinModelPortaliP93746.
SMRiP93746. Positions 11-39.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT2G42000.2.

Proteomic databases

PaxDbiP93746.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G42000.1; AT2G42000.1; AT2G42000.
GeneIDi818800.
GrameneiAT2G42000.1; AT2G42000.1; AT2G42000.

Organism-specific databases

TAIRiAT2G42000.

Phylogenomic databases

eggNOGiENOG410J62V. Eukaryota.
ENOG410Z4N5. LUCA.
HOGENOMiHOG000243560.
InParanoidiP93746.
PhylomeDBiP93746.

Miscellaneous databases

PROiP93746.

Gene expression databases

ExpressionAtlasiP93746. differential.
GenevisibleiP93746. AT.

Family and domain databases

InterProiIPR000316. Metallthion_15.
[Graphical view]
PfamiPF02068. Metallothio_PEC. 1 hit.
[Graphical view]
PRINTSiPR00877. MTPLANTPEC.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 3-84.
    Strain: cv. Columbia.
    Tissue: Dry seed.
  4. "Examining the specific contributions of individual Arabidopsis metallothioneins to copper distribution and metal tolerance."
    Guo W.J., Meetam M., Goldsbrough P.B.
    Plant Physiol. 146:1697-1706(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "Type 4 metallothionein genes are involved in regulating Zn ion accumulation in late embryo and in controlling early seedling growth in Arabidopsis."
    Ren Y., Liu Y., Chen H., Li G., Zhang X., Zhao J.
    Plant Cell Environ. 35:770-789(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION.

Entry informationi

Entry nameiMT4A_ARATH
AccessioniPrimary (citable) accession number: P93746
Secondary accession number(s): F4ILY8, Q42258
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: February 17, 2016
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Plants silencing both MT4A and MT4B have reduced seed weight and reduced seedling growth after germination.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Metallothioneins
    Classification of metallothioneins and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.