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Protein

Valine--tRNA ligase, mitochondrial 1

Gene

TWN2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Required for embryo development and seed viability.1 Publication

Catalytic activityi

ATP + L-valine + tRNA(Val) = AMP + diphosphate + L-valyl-tRNA(Val).Curated

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei698 – 6981ATPBy similarity

GO - Molecular functioni

GO - Biological processi

  • embryo development ending in seed dormancy Source: TAIR
  • valyl-tRNA aminoacylation Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Developmental protein, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT1G14610-MONOMER.
BRENDAi6.1.1.9. 399.

Names & Taxonomyi

Protein namesi
Recommended name:
Valine--tRNA ligase, mitochondrial 1Curated (EC:6.1.1.9Curated)
Alternative name(s):
AtSYV11 Publication
Protein TWIN 2Curated
Valyl-tRNA synthetaseCurated
Short name:
ValRSCurated
Gene namesi
Name:TWN21 Publication
Ordered Locus Names:At1g14610
ORF Names:T5E21.11
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G14610.

Subcellular locationi

GO - Cellular componenti

  • chloroplast Source: TAIR
  • cytosol Source: TAIR
  • mitochondrion Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4646MitochondrionSequence analysisAdd
BLAST
Chaini47 – 11081062Valine--tRNA ligase, mitochondrial 1Sequence analysisPRO_0000106258Add
BLAST

Proteomic databases

PaxDbiP93736.
PRIDEiP93736.

Expressioni

Gene expression databases

GenevisibleiP93736. AT.

Interactioni

Protein-protein interaction databases

BioGridi23263. 1 interaction.
STRINGi3702.AT1G14610.1.

Structurei

3D structure databases

ProteinModelPortaliP93736.
SMRiP93736. Positions 136-1087.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili1032 – 106433Sequence analysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi177 – 18711"HIGH" regionCuratedAdd
BLAST
Motifi695 – 6995"KMSKS" regionCurated

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi50 – 10859Lys-richPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Coiled coil, Transit peptide

Phylogenomic databases

eggNOGiKOG0432. Eukaryota.
COG0525. LUCA.
HOGENOMiHOG000020094.
InParanoidiP93736.
KOiK01873.
OMAiFGWPNQS.
PhylomeDBiP93736.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02004. Val_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR002303. Valyl-tRNA_ligase.
[Graphical view]
PANTHERiPTHR11946:SF5. PTHR11946:SF5. 3 hits.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00986. TRNASYNTHVAL.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00422. valS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P93736-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLLFLRRAK PLFVSCCSAT HSRSSFLSPT LTNQLVRSFH GSRTMSESEK
60 70 80 90 100
KILTEEELER KKKKEEKAKE KELKKQKALE KERLAELKAK QAKDGTNVPK
110 120 130 140 150
KSAKKSSKRD ASEENPEDFV DPETPLGERK RLSSQMAKQY SPATVEKSWY
160 170 180 190 200
AWWEKSDLFK ADAKSSKPPF VIVLPPPNVT GALHIGHALT SAIEDTIIRW
210 220 230 240 250
KRMSGYNALW VPGVDHAGIA TQVVVEKKIM RDRGMTRHDV GREEFVKEVW
260 270 280 290 300
KWKNQYGGTI LTQLRRLGAS LDWSRECFTM DEQRSKAVTE AFVRLYKEGL
310 320 330 340 350
IYRDIRLVNW DCILRTAISD VEVEYIDIKE KTLLKVPGYE KPVEFGLLTS
360 370 380 390 400
FAYPLEGGLG EVIVATTRVE TMLGDTAIAI HPDDARYKHL HGKFAVHPFN
410 420 430 440 450
GRKLPIICDG ILVDPNFGTG CVKITPAHDP NDCEVGKRHK LEFINIFTDD
460 470 480 490 500
GKINTNGGSD FAGMPRFAAR EAVVEALQKQ GLYRGAKNNE MRLGLCSRTN
510 520 530 540 550
DVIEPMIKPQ WYVNCSMIGK EALDVAITDE NKKLEFVPKQ YTAEWRRWLE
560 570 580 590 600
NIRDWCISRQ LWWGHRIPAW YATLEEDQLK EVGAYSDHWV VARTEDDARE
610 620 630 640 650
EAAQKFLGKK FELTRDPDVL DTWFSSGLFP LSVLGWPDVT DDFKAFYPTS
660 670 680 690 700
VLETGHDILF FWVARMVMMG MKLGGEVPFS KVYFHPMIRD AHGRKMSKSL
710 720 730 740 750
GNVIDPLEVI NGVTLEGLHK RLEEGNLDPK EVIVAKEGQV KDFPNGIPEC
760 770 780 790 800
GTDALRFALV SYTAQSDKIN LDILRVVGYR QWCNKLWNAV RFAMMKLGDG
810 820 830 840 850
YTPPQTLSPE TMPFSCQWIL SVLNKAISKT VVSLDAFEFS DAANTIYAWW
860 870 880 890 900
QYQFCDVYIE AIKPYFAGDN PTFASERAHA QHALWISLET GLRLLHPFMP
910 920 930 940 950
FVTEELWQRL PAPKDTERKA SIMICDYPSA IENWSNEKVE SEMDTVLATV
960 970 980 990 1000
KCMRALRAGL LEKQKNERLP AFALCENNVT SEIVKSHELE IRTLANLSSL
1010 1020 1030 1040 1050
EVVSKGQHAA PPGSSVETVN ENLKVYLEVD GAINTEAEQE KIRNKIGELQ
1060 1070 1080 1090 1100
KQKEKLQKMM SVSTYEEKVP ANIKEDNANK LAKILQEFDF FEKESARLAA

ETSNSGNQ
Length:1,108
Mass (Da):125,926
Last modified:June 20, 2002 - v2
Checksum:i590254624020F494
GO

Sequence cautioni

The sequence AAB49704.1 differs from that shown. Reason: Frameshift at positions 357 and 361. Curated
The sequence AAF63175.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti357 – 3615GGLGE → ELGR (PubMed:9207094).Curated
Sequence conflicti716 – 7161E → G in AAB49704 (PubMed:9207094).Curated
Sequence conflicti799 – 7991D → S in AAB49704 (PubMed:9207094).Curated
Sequence conflicti830 – 8301T → A in AAB49704 (PubMed:9207094).Curated
Sequence conflicti1028 – 10281E → K in AAB49704 (PubMed:9207094).Curated
Sequence conflicti1063 – 10631S → F in AAB49704 (PubMed:9207094).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U89986 mRNA. Translation: AAB49704.1. Frameshift.
BT010735 mRNA. Translation: AAR23705.1.
AC010657 Genomic DNA. Translation: AAF63175.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE29190.1.
AY062693 mRNA. Translation: AAL32771.1.
U93308 Genomic DNA. Translation: AAB51589.1.
PIRiF86280.
RefSeqiNP_172913.1. NM_101328.3.
UniGeneiAt.16600.

Genome annotation databases

EnsemblPlantsiAT1G14610.1; AT1G14610.1; AT1G14610.
GeneIDi838023.
GrameneiAT1G14610.1; AT1G14610.1; AT1G14610.
KEGGiath:AT1G14610.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U89986 mRNA. Translation: AAB49704.1. Frameshift.
BT010735 mRNA. Translation: AAR23705.1.
AC010657 Genomic DNA. Translation: AAF63175.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE29190.1.
AY062693 mRNA. Translation: AAL32771.1.
U93308 Genomic DNA. Translation: AAB51589.1.
PIRiF86280.
RefSeqiNP_172913.1. NM_101328.3.
UniGeneiAt.16600.

3D structure databases

ProteinModelPortaliP93736.
SMRiP93736. Positions 136-1087.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi23263. 1 interaction.
STRINGi3702.AT1G14610.1.

Proteomic databases

PaxDbiP93736.
PRIDEiP93736.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G14610.1; AT1G14610.1; AT1G14610.
GeneIDi838023.
GrameneiAT1G14610.1; AT1G14610.1; AT1G14610.
KEGGiath:AT1G14610.

Organism-specific databases

TAIRiAT1G14610.

Phylogenomic databases

eggNOGiKOG0432. Eukaryota.
COG0525. LUCA.
HOGENOMiHOG000020094.
InParanoidiP93736.
KOiK01873.
OMAiFGWPNQS.
PhylomeDBiP93736.

Enzyme and pathway databases

BioCyciARA:AT1G14610-MONOMER.
BRENDAi6.1.1.9. 399.

Miscellaneous databases

PROiP93736.

Gene expression databases

GenevisibleiP93736. AT.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02004. Val_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR002303. Valyl-tRNA_ligase.
[Graphical view]
PANTHERiPTHR11946:SF5. PTHR11946:SF5. 3 hits.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00986. TRNASYNTHVAL.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00422. valS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Suspensor-derived polyembryony caused by altered expression of valyl-tRNA synthetase in the twn2 mutant of Arabidopsis."
    Zhang J.Z., Somerville C.R.
    Proc. Natl. Acad. Sci. U.S.A. 94:7349-7355(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION.
    Strain: cv. Columbia.
  2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 3-1108.
    Strain: cv. Columbia.
  5. "Arabidopsis cDNA clones."
    Cheuk R.F., Chen H., Kim C.J., Shinn P., Carninci P., Hayashizaki Y., Ishida J., Kamiya A., Kawai J., Narusaka M., Sakurai T., Satou M., Seki M., Shinozaki K., Ecker J.R.
    Submitted (NOV-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 45-1108.
    Strain: cv. Columbia.
  6. "Characterization of two bifunctional Arabdopsis thaliana genes coding for mitochondrial and cytosolic forms of valyl-tRNA synthetase and threonyl-tRNA synthetase by alternative use of two in-frame AUGs."
    Souciet G., Menand B., Ovesna J., Cosset A., Dietrich A., Wintz H.
    Eur. J. Biochem. 266:848-854(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiSYVM1_ARATH
AccessioniPrimary (citable) accession number: P93736
Secondary accession number(s): Q6NPT2, Q8W4A8, Q9MA22
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: June 20, 2002
Last modified: February 17, 2016
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.