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Protein

Proline iminopeptidase

Gene

PIP

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Specifically catalyzes the removal of N-terminal proline residues from peptides.By similarity

Catalytic activityi

Release of N-terminal proline from a peptide.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei172 – 1721NucleophileBy similarity
Active sitei329 – 3291By similarity
Active sitei357 – 3571Proton donorBy similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Protease

Enzyme and pathway databases

BioCyciARA:AT2G14260-MONOMER.
ARA:GQT-1502-MONOMER.

Protein family/group databases

ESTHERiarath-pip. Proline_iminopeptidase.
MEROPSiS33.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Proline iminopeptidase (EC:3.4.11.5)
Short name:
PIP
Alternative name(s):
Prolyl aminopeptidase
Short name:
PAP
Gene namesi
Name:PIP
Ordered Locus Names:At2g14260
ORF Names:T1O16.15
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G14260.

Subcellular locationi

GO - Cellular componenti

  • chloroplast Source: TAIR
  • cytosol Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 380380Proline iminopeptidasePRO_0000080853Add
BLAST

Proteomic databases

PaxDbiP93732.
PRIDEiP93732.

Expressioni

Gene expression databases

ExpressionAtlasiP93732. baseline and differential.
GenevisibleiP93732. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G14260.1.

Structurei

3D structure databases

ProteinModelPortaliP93732.
SMRiP93732. Positions 64-373.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S33 family.Curated

Phylogenomic databases

eggNOGiENOG410IG32. Eukaryota.
ENOG410XPKQ. LUCA.
HOGENOMiHOG000171480.
InParanoidiP93732.
KOiK01259.
OMAiVSEMFPD.
PhylomeDBiP93732.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR002410. Peptidase_S33.
IPR005944. Pro_iminopeptidase.
[Graphical view]
PfamiPF00561. Abhydrolase_1. 1 hit.
[Graphical view]
PRINTSiPR00111. ABHYDROLASE.
PR00793. PROAMNOPTASE.
SUPFAMiSSF53474. SSF53474. 1 hit.
TIGRFAMsiTIGR01249. pro_imino_pep_1. 1 hit.

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: P93732-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNLILASFSL APCFCVRFFP SNHNNLNLLF PGQRKIQVSC GGKSEVLKSD
60 70 80 90 100
TMEPHEAETF VNKRTLYAPI EPYSSGNLKV SDVHTLYWEQ SGKPDGHPVV
110 120 130 140 150
FLHGGPGGGT APSNRRFFDP EFYRIVLFDQ RGAGKSTPHA CLEENTTWDL
160 170 180 190 200
VNDIEKLREH LKIPEWLVFG GSWGSTLALA YSQSHPDKVT GLVLRGIFLL
210 220 230 240 250
RKKEIDWFYE GGAAAIYPDA WEEFRDLIPE NERGSSLVDA YHKRLNSDDL
260 270 280 290 300
EIQYAAARAW TKWEMMTAYL RPNLENVQKA EDDKFSLAFA RIENHYFVNK
310 320 330 340 350
GFFPSDSHLL DNVDKIRHIK TTIVQGRYDV CCPMMSAWDL HKAWPEAELK
360 370 380
IVYDAGHSAN EPGISAELVV ANEKMKALMG
Length:380
Mass (Da):43,059
Last modified:May 29, 2007 - v3
Checksum:iEC7DA93283639A6D
GO

Sequence cautioni

The sequence AAC49560.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti28 – 281L → P (Ref. 4) Curated
Sequence conflicti344 – 3441W → C in AAC49560 (Ref. 4) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC006304 Genomic DNA. Translation: AAD20113.1.
CP002685 Genomic DNA. Translation: AEC06293.1.
AY059916 mRNA. Translation: AAL24398.1.
AY114690 mRNA. Translation: AAM48009.1.
U72711 mRNA. Translation: AAC49560.1. Different initiation.
PIRiE84515.
RefSeqiNP_179037.2. NM_126994.3. [P93732-1]
NP_973454.1. NM_201725.2.
UniGeneiAt.22017.

Genome annotation databases

EnsemblPlantsiAT2G14260.1; AT2G14260.1; AT2G14260. [P93732-1]
GeneIDi815913.
KEGGiath:AT2G14260.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC006304 Genomic DNA. Translation: AAD20113.1.
CP002685 Genomic DNA. Translation: AEC06293.1.
AY059916 mRNA. Translation: AAL24398.1.
AY114690 mRNA. Translation: AAM48009.1.
U72711 mRNA. Translation: AAC49560.1. Different initiation.
PIRiE84515.
RefSeqiNP_179037.2. NM_126994.3. [P93732-1]
NP_973454.1. NM_201725.2.
UniGeneiAt.22017.

3D structure databases

ProteinModelPortaliP93732.
SMRiP93732. Positions 64-373.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT2G14260.1.

Protein family/group databases

ESTHERiarath-pip. Proline_iminopeptidase.
MEROPSiS33.001.

Proteomic databases

PaxDbiP93732.
PRIDEiP93732.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G14260.1; AT2G14260.1; AT2G14260. [P93732-1]
GeneIDi815913.
KEGGiath:AT2G14260.

Organism-specific databases

TAIRiAT2G14260.

Phylogenomic databases

eggNOGiENOG410IG32. Eukaryota.
ENOG410XPKQ. LUCA.
HOGENOMiHOG000171480.
InParanoidiP93732.
KOiK01259.
OMAiVSEMFPD.
PhylomeDBiP93732.

Enzyme and pathway databases

BioCyciARA:AT2G14260-MONOMER.
ARA:GQT-1502-MONOMER.

Miscellaneous databases

PROiP93732.

Gene expression databases

ExpressionAtlasiP93732. baseline and differential.
GenevisibleiP93732. AT.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR002410. Peptidase_S33.
IPR005944. Pro_iminopeptidase.
[Graphical view]
PfamiPF00561. Abhydrolase_1. 1 hit.
[Graphical view]
PRINTSiPR00111. ABHYDROLASE.
PR00793. PROAMNOPTASE.
SUPFAMiSSF53474. SSF53474. 1 hit.
TIGRFAMsiTIGR01249. pro_imino_pep_1. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. Tamura T., Tamura N., Lottspeich F., Baumeister W.
    Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 18-380.
    Strain: cv. Columbia.

Entry informationi

Entry nameiPIP_ARATH
AccessioniPrimary (citable) accession number: P93732
Secondary accession number(s): Q93Y05, Q9SKJ3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: May 29, 2007
Last modified: November 11, 2015
This is version 122 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.