Reviewed, UniProtKB/Swiss-Prot P93732 (PIP_ARATH)

Names and origin

Protein names

Recommended name:
    Proline iminopeptidase
      Short name=PIP
    EC=3.4.11.5
Alternative name(s):
    Prolyl aminopeptidase
      Short name=PAP

Gene names

Name:	PIP
Ordered Locus Names:	At2g14260
ORF Names:	T1O16.15

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	380 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Specifically catalyzes the removal of N-terminal proline residues from peptides By similarity.
Catalytic activity	Release of N-terminal proline from a peptide.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the peptidase S33 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	chloroplast Inferred from direct assay. Source: TAIR
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: P93732-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 380	380	Proline iminopeptidase		PRO_0000080853
Sites
	Active site	172	1	Nucleophile By similarity
	Active site	329	1	By similarity
	Active site	357	1	Proton donor By similarity
Experimental info
	Sequence conflict	28	1	L → P Ref.3
	Sequence conflict	344	1	W → C in AAC49560. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 29, 2007. Version 3. Checksum: EC7DA93283639A6D	FASTA	380	43,059
	10 20 30 40 50 60 MNLILASFSL APCFCVRFFP SNHNNLNLLF PGQRKIQVSC GGKSEVLKSD TMEPHEAETF 70 80 90 100 110 120 VNKRTLYAPI EPYSSGNLKV SDVHTLYWEQ SGKPDGHPVV FLHGGPGGGT APSNRRFFDP 130 140 150 160 170 180 EFYRIVLFDQ RGAGKSTPHA CLEENTTWDL VNDIEKLREH LKIPEWLVFG GSWGSTLALA 190 200 210 220 230 240 YSQSHPDKVT GLVLRGIFLL RKKEIDWFYE GGAAAIYPDA WEEFRDLIPE NERGSSLVDA 250 260 270 280 290 300 YHKRLNSDDL EIQYAAARAW TKWEMMTAYL RPNLENVQKA EDDKFSLAFA RIENHYFVNK 310 320 330 340 350 360 GFFPSDSHLL DNVDKIRHIK TTIVQGRYDV CCPMMSAWDL HKAWPEAELK IVYDAGHSAN 370 380 EPGISAELVV ANEKMKALMG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[3]	Tamura T., Tamura N., Lottspeich F., Baumeister W. Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 18-380. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC006304 Genomic DNA. Translation: AAD20113.1. AY059916 mRNA. Translation: AAL24398.1. AY114690 mRNA. Translation: AAM48009.1. U72711 mRNA. Translation: AAC49560.1. Different initiation.
IPI	IPI00518206.
PIR	E84515.
RefSeq	NP_179037.2. NP_973454.1.
UniGene	At.22017 Rra.3486
3D structure databases
SMR	P93732. Positions 64-375.
ModBase	Search...
Protein-protein interaction databases
STRING	P93732.
Protein family/group databases
MEROPS	S33.001.
Proteomic databases
PRIDE	P93732.
Genome annotation databases
GeneID	815913.
GenomeReviews	Gene locus AT2G14260 in contig CT485783_GR.
KEGG	ath:AT2G14260.
Organism-specific databases
TAIR	At2g14260.
Phylogenomic databases
eggNOG	euNOG04774.
HOGENOM	HBG677789.
InParanoid	P93732.
OMA	ANTTWHL.
Enzyme and pathway databases
BRENDA	3.4.11.5. 302.
Gene expression databases
Genevestigator	P93732.
Family and domain databases
InterPro	IPR000073. AB_hydrolase_1. IPR005944. Pept_S33. IPR002410. Peptidase_S33. [Graphical view]
Pfam	PF00561. Abhydrolase_1. 1 hit. [Graphical view]
PRINTS	PR00793. PROAMNOPTASE.
TIGRFAMs	TIGR01249. pro_imino_pep_1. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PIP_ARATH

Accession

Primary (citable) accession number: P93732
Secondary accession number(s): Q93Y05, Q9SKJ3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	May 29, 2007
Last modified:	February 9, 2010
This is version 82 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)