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P93665 (DCS1_GOSHI) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
(+)-delta-cadinene synthase

Short name=D-cadinene synthase
EC=4.2.3.13
Gene names
Name:CDN1
OrganismGossypium hirsutum (Upland cotton) (Gossypium mexicanum)
Taxonomic identifier3635 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsMalvalesMalvaceaeMalvoideaeGossypium

Protein attributes

Sequence length554 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Responsible for the cyclization of trans,trans-farnesyl diphosphate (FPP) to (+)-delta cadinene.

Catalytic activity

(2E,6E)-farnesyl diphosphate = (+)-delta-cadinene + diphosphate.

Cofactor

Binds 3 magnesium ions per subunit By similarity.

Pathway

Secondary metabolite biosynthesis; terpenoid biosynthesis.

Induction

By bacterial infection.

Domain

The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity, presumably through binding to Mg2+.

Sequence similarities

Belongs to the terpene synthase family.

Ontologies

Keywords
   LigandMagnesium
Metal-binding
   Molecular functionLyase
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological_processterpenoid biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_function(+)-delta-cadinene synthase activity

Inferred from electronic annotation. Source: UniProtKB-EC

magnesium ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 554554(+)-delta-cadinene synthase
PRO_0000186443

Regions

Motif307 – 3115DDXXD motif

Sites

Metal binding3071Magnesium 1 By similarity
Metal binding3071Magnesium 2 By similarity
Metal binding3111Magnesium 1 By similarity
Metal binding3111Magnesium 2 By similarity
Metal binding4511Magnesium 3 By similarity
Metal binding4551Magnesium 3 By similarity

Sequences

Sequence LengthMass (Da)Tools
P93665 [UniParc].

Last modified May 1, 1997. Version 1.
Checksum: 8BCC78AD8CA5B816

FASTA55464,020
        10         20         30         40         50         60 
MASQVSQMPS SSPLSSNKDE MRPKADFQPS IWGDFFLNCP DKNIDAETQK RHQQLKEEVR 

        70         80         90        100        110        120 
KMIVAPMANS TLKLAFIDSV QGLGVSYHFT KEIEDELENI YHNNNDAEND LYTTSLRFRL 

       130        140        150        160        170        180 
LREHGFHVSC DVFNKFKDEQ GNFKSSVTSD VRGLLELYQA SYLRVHGEDI LDEAISFTSN 

       190        200        210        220        230        240 
HLSLAVASLD HPLSEEVSHA LKQSIRRGLP RVEARHYLSV YQDIESHNKV LLEFAKIDFN 

       250        260        270        280        290        300 
MVQLLHRKEL SEISRWWKDL DFQRKLPYAR DRVVEGYFWI SGVYFEPQYS LGRKMLTKVI 

       310        320        330        340        350        360 
AMASIVDDTY DSYATYEELI PYTNAIERWD IKCIDELPEY MKPSYKALLD VYEEMEQLVA 

       370        380        390        400        410        420 
EHGRQYRVEY AKNAMIRLAQ SYLVEARWTL QNYKPSFEEF KANALPTCGY AMLAITSFVG 

       430        440        450        460        470        480 
MGDIVTPETF KWAANDPKII QASTIICRFM DDVTEHKFKH RREDDCSAIE CYMEEYGVTA 

       490        500        510        520        530        540 
QEAYDVFNKH VESAWKDVNQ GFLKPTEMPT EVLNRSLNLA RVMDVLYREG DGYTYVGKAA 

       550 
KGGITSLLIE PIAL 

« Hide

References

[1]"cDNA sequence of a (+)-delta-cadinene synthase gene induced in Gossypium hirsutum L. by bacterial infection."
Davis E.M., Chen Y.-S., Essenberg M., Pierce M.L.
Plant Gene Register PGR98-040
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Westburn M.
[2]"Purification of (+)-delta-cadinene synthase, a sesquiterpene cyclase from bacteria-inoculated cotton foliar tissue."
Davis E.M., Tsuji J., Davis G.D., Pierce M.L., Essenberg M.
Phytochemistry 41:1047-1055(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: PARTIAL PROTEIN SEQUENCE, CHARACTERIZATION.

Web resources

Wikipedia

Delta-cadinene synthase entry

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U88318 mRNA. Translation: AAC12784.1.

3D structure databases

ProteinModelPortalP93665.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00213.

Family and domain databases

Gene3D1.10.600.10. 1 hit.
1.50.30.10. 1 hit.
InterProIPR001906. Terpene_synth_N.
IPR005630. Terpene_synthase_metal-bd.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
IPR008949. Terpenoid_synth.
[Graphical view]
PfamPF01397. Terpene_synth. 1 hit.
PF03936. Terpene_synth_C. 1 hit.
[Graphical view]
SUPFAMSSF48239. SSF48239. 1 hit.
SSF48576. SSF48576. 1 hit.
ProtoNetSearch...

Entry information

Entry nameDCS1_GOSHI
AccessionPrimary (citable) accession number: P93665
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: May 1, 1997
Last modified: February 19, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways