P93665 (DCS1_GOSHI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
(+)-delta-cadinene synthase
Short name=D-cadinene synthase
EC=4.2.3.13

Gene names

Name:

CDN1

Organism

Gossypium hirsutum (Upland cotton) (Gossypium mexicanum)

Taxonomic identifier

3635 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Malvales › Malvaceae › Malvoideae › Gossypium

Protein attributes

Sequence length	554 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Responsible for the cyclization of trans,trans-farnesyl diphosphate (FPP) to (+)-delta cadinene.
Catalytic activity	(2E,6E)-farnesyl diphosphate = (+)-delta-cadinene + diphosphate.
Cofactor	Binds 3 magnesium ions per subunit By similarity.
Pathway	Secondary metabolite biosynthesis; terpenoid biosynthesis.
Induction	By bacterial infection.
Domain	The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity, presumably through binding to Mg2+.
Sequence similarities	Belongs to the terpene synthase family.

Ontologies

Keywords
Ligand	Magnesium Metal-binding
Molecular function	Lyase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	terpenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	(+)-delta-cadinene synthase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 554	554	(+)-delta-cadinene synthase		PRO_0000186443
Regions
	Motif	307 – 311	5	DDXXD motif
Sites
	Metal binding	307	1	Magnesium 1 By similarity
	Metal binding	307	1	Magnesium 2 By similarity
	Metal binding	311	1	Magnesium 1 By similarity
	Metal binding	311	1	Magnesium 2 By similarity
	Metal binding	451	1	Magnesium 3 By similarity
	Metal binding	455	1	Magnesium 3 By similarity

Sequences

	Sequence		Length	Mass (Da)
	P93665 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 8BCC78AD8CA5B816	FASTA	554	64,020
	10 20 30 40 50 60 MASQVSQMPS SSPLSSNKDE MRPKADFQPS IWGDFFLNCP DKNIDAETQK RHQQLKEEVR 70 80 90 100 110 120 KMIVAPMANS TLKLAFIDSV QGLGVSYHFT KEIEDELENI YHNNNDAEND LYTTSLRFRL 130 140 150 160 170 180 LREHGFHVSC DVFNKFKDEQ GNFKSSVTSD VRGLLELYQA SYLRVHGEDI LDEAISFTSN 190 200 210 220 230 240 HLSLAVASLD HPLSEEVSHA LKQSIRRGLP RVEARHYLSV YQDIESHNKV LLEFAKIDFN 250 260 270 280 290 300 MVQLLHRKEL SEISRWWKDL DFQRKLPYAR DRVVEGYFWI SGVYFEPQYS LGRKMLTKVI 310 320 330 340 350 360 AMASIVDDTY DSYATYEELI PYTNAIERWD IKCIDELPEY MKPSYKALLD VYEEMEQLVA 370 380 390 400 410 420 EHGRQYRVEY AKNAMIRLAQ SYLVEARWTL QNYKPSFEEF KANALPTCGY AMLAITSFVG 430 440 450 460 470 480 MGDIVTPETF KWAANDPKII QASTIICRFM DDVTEHKFKH RREDDCSAIE CYMEEYGVTA 490 500 510 520 530 540 QEAYDVFNKH VESAWKDVNQ GFLKPTEMPT EVLNRSLNLA RVMDVLYREG DGYTYVGKAA 550 KGGITSLLIE PIAL

References

[1]	"cDNA sequence of a (+)-delta-cadinene synthase gene induced in Gossypium hirsutum L. by bacterial infection." Davis E.M., Chen Y.-S., Essenberg M., Pierce M.L. Plant Gene Register PGR98-040 Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Westburn M.
[2]	"Purification of (+)-delta-cadinene synthase, a sesquiterpene cyclase from bacteria-inoculated cotton foliar tissue." Davis E.M., Tsuji J., Davis G.D., Pierce M.L., Essenberg M. Phytochemistry 41:1047-1055(1996) [PubMed] [Europe PMC] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE, CHARACTERIZATION.

Web resources

Wikipedia

Delta-cadinene synthase entry

Cross-references

Sequence databases
EMBL GenBank DDBJ	U88318 mRNA. Translation: AAC12784.1.
3D structure databases
ProteinModelPortal	P93665.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00213.
Family and domain databases
Gene3D	1.10.600.10. 1 hit. 1.50.30.10. 1 hit.
InterPro	IPR001906. Terpene_synth_N. IPR005630. Terpene_synthase_metal-bd. IPR008930. Terpenoid_cyclase/PrenylTrfase. IPR008949. Terpenoid_synth. [Graphical view]
Pfam	PF01397. Terpene_synth. 1 hit. PF03936. Terpene_synth_C. 1 hit. [Graphical view]
SUPFAM	SSF48239. Terp_cyc_toroid. 1 hit. SSF48576. Terpenoid_synth. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DCS1_GOSHI

Accession

Primary (citable) accession number: P93665

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	May 1, 1997
Last modified:	May 1, 2013
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program