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Protein

Syntaxin-22

Gene

SYP22

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May provide the t-SNARE function in the vacuolar assembly. Promotes the formation of vacuolar membrane 'bulbs'. Required for inflorescence stem gravitropism.2 Publications

GO - Molecular functioni

GO - Biological processi

  • amyloplast organization Source: TAIR
  • intracellular protein transport Source: InterPro
  • negative gravitropism Source: TAIR
  • stomatal movement Source: TAIR
  • vesicle docking Source: GO_Central
  • vesicle fusion Source: GO_Central
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Syntaxin-22
Short name:
AtSYP22
Short name:
AtVAM3
Alternative name(s):
Protein SHOOT GRAVITROPISM 3
Gene namesi
Name:SYP22
Synonyms:SGR3, VAM3
Ordered Locus Names:At5g46860
ORF Names:MSD23.9
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G46860.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 246245CytoplasmicSequence analysisAdd
BLAST
Transmembranei247 – 26721Helical; Anchor for type IV membrane proteinSequence analysisAdd
BLAST
Topological domaini268 – 2681VesicularSequence analysis

GO - Cellular componenti

  • integral component of membrane Source: GO_Central
  • late endosome membrane Source: UniProtKB-SubCell
  • plant-type vacuole Source: TAIR
  • plant-type vacuole membrane Source: TAIR
  • SNARE complex Source: GO_Central
  • vacuole Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Vacuole

Pathology & Biotechi

Disruption phenotypei

The sgr3-1 mutant has a reduced gravitropic response in inflorescence stem but a normal gravitropic response in hypocotyls. Reduced formation of vacuolar membrane 'bulbs'.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCombined sources
Chaini2 – 268267Syntaxin-22PRO_0000210254Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineCombined sources

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP93654.
PRIDEiP93654.

PTM databases

iPTMnetiP93654.

Expressioni

Tissue specificityi

Expressed in roots, leaves, stems, flower and green siliques.

Gene expression databases

GenevisibleiP93654. AT.

Interactioni

Subunit structurei

Interacts with VTI11 and SYP51 to form a t-SNARE complex, but not with VPS45.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
VTI11Q9SEL63EBI-2352632,EBI-1162795

GO - Molecular functioni

Protein-protein interaction databases

BioGridi19978. 58 interactions.
IntActiP93654. 54 interactions.
STRINGi3702.AT5G46860.1.

Structurei

3D structure databases

ProteinModelPortaliP93654.
SMRiP93654. Positions 181-244.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini175 – 23763t-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the syntaxin family.Curated
Contains 1 t-SNARE coiled-coil homology domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0811. Eukaryota.
COG5325. LUCA.
HOGENOMiHOG000188453.
InParanoidiP93654.
KOiK08488.
OMAiKTRAHIT.
PhylomeDBiP93654.

Family and domain databases

InterProiIPR006012. Syntaxin/epimorphin_CS.
IPR006011. Syntaxin_N.
IPR010989. t-SNARE.
IPR000727. T_SNARE_dom.
[Graphical view]
PfamiPF05739. SNARE. 1 hit.
[Graphical view]
SMARTiSM00503. SynN. 1 hit.
SM00397. t_SNARE. 1 hit.
[Graphical view]
SUPFAMiSSF47661. SSF47661. 1 hit.
PROSITEiPS00914. SYNTAXIN. 1 hit.
PS50192. T_SNARE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P93654-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFQDLESGR GRSTRKFNGG RQDSTQAVAS GIFQINTGVS TFQRLVNTLG
60 70 80 90 100
TPKDTPELRE KLHKTRLHIG QLVKDTSAKL KEASETDHQS GVNPSKKIAD
110 120 130 140 150
AKLARDFQAV LKEFQKAQQT AAERETTYTP FVPQSALPSS YTAGEVDKVP
160 170 180 190 200
EQRAQLQESK RQELVLLDNE IAFNEAVIEE REQGIQEIHQ QIGEVNEIFK
210 220 230 240 250
DLAVLVNDQG VMIDDIGTHI DNSRAATSQG KSQLVQAAKT QKSNSSLTCL
260
LLVIFGIVLL IVIIVLAA
Length:268
Mass (Da):29,481
Last modified:May 1, 1997 - v1
Checksum:iF908E43A42470BEE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U88045 mRNA. Translation: AAC49823.1.
AB022221 Genomic DNA. Translation: BAA97220.1.
CP002688 Genomic DNA. Translation: AED95437.1.
AF385695 mRNA. Translation: AAK60288.1.
AY072015 mRNA. Translation: AAL57708.1.
AY133666 mRNA. Translation: AAM91496.1.
AY085121 mRNA. Translation: AAM61675.1.
RefSeqiNP_568671.1. NM_124057.3.
UniGeneiAt.22922.
At.50510.

Genome annotation databases

EnsemblPlantsiAT5G46860.1; AT5G46860.1; AT5G46860.
GeneIDi834730.
GrameneiAT5G46860.1; AT5G46860.1; AT5G46860.
KEGGiath:AT5G46860.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U88045 mRNA. Translation: AAC49823.1.
AB022221 Genomic DNA. Translation: BAA97220.1.
CP002688 Genomic DNA. Translation: AED95437.1.
AF385695 mRNA. Translation: AAK60288.1.
AY072015 mRNA. Translation: AAL57708.1.
AY133666 mRNA. Translation: AAM91496.1.
AY085121 mRNA. Translation: AAM61675.1.
RefSeqiNP_568671.1. NM_124057.3.
UniGeneiAt.22922.
At.50510.

3D structure databases

ProteinModelPortaliP93654.
SMRiP93654. Positions 181-244.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi19978. 58 interactions.
IntActiP93654. 54 interactions.
STRINGi3702.AT5G46860.1.

PTM databases

iPTMnetiP93654.

Proteomic databases

PaxDbiP93654.
PRIDEiP93654.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G46860.1; AT5G46860.1; AT5G46860.
GeneIDi834730.
GrameneiAT5G46860.1; AT5G46860.1; AT5G46860.
KEGGiath:AT5G46860.

Organism-specific databases

TAIRiAT5G46860.

Phylogenomic databases

eggNOGiKOG0811. Eukaryota.
COG5325. LUCA.
HOGENOMiHOG000188453.
InParanoidiP93654.
KOiK08488.
OMAiKTRAHIT.
PhylomeDBiP93654.

Miscellaneous databases

PROiP93654.

Gene expression databases

GenevisibleiP93654. AT.

Family and domain databases

InterProiIPR006012. Syntaxin/epimorphin_CS.
IPR006011. Syntaxin_N.
IPR010989. t-SNARE.
IPR000727. T_SNARE_dom.
[Graphical view]
PfamiPF05739. SNARE. 1 hit.
[Graphical view]
SMARTiSM00503. SynN. 1 hit.
SM00397. t_SNARE. 1 hit.
[Graphical view]
SUPFAMiSSF47661. SSF47661. 1 hit.
PROSITEiPS00914. SYNTAXIN. 1 hit.
PS50192. T_SNARE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The AtVAM3 encodes a syntaxin-related molecule implicated in the vacuolar assembly in Arabidopsis thaliana."
    Sato M.H., Nakamura N., Ohsumi Y., Kouchi H., Kondo M., Hara-Nishimura I., Nishimura M., Wada Y.
    J. Biol. Chem. 272:24530-24535(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
  2. "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones."
    Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S.
    DNA Res. 7:31-63(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  6. "SGR1, SGR2, SGR3: novel genetic loci involved in shoot gravitropism in Arabidopsis thaliana."
    Fukaki H., Fujisawa H., Tasaka M.
    Plant Physiol. 110:945-955(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    Strain: cv. Columbia.
  7. "The t-SNARE AtVAM3p resides on the prevacuolar compartment in Arabidopsis root cells."
    Sanderfoot A.A., Kovaleva V., Zheng H., Raikhel N.V.
    Plant Physiol. 121:929-938(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  8. Cited for: LACK OF INTERACTION WITH VPS45.
  9. "Interactions between syntaxins identify at least five SNARE complexes within the Golgi/prevacuolar system of the Arabidopsis cell."
    Sanderfoot A.A., Kovaleva V., Bassham D.C., Raikhel N.V.
    Mol. Biol. Cell 12:3733-3743(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH VTI11 AND SYP51.
  10. "The occurrence of 'bulbs', a complex configuration of the vacuolar membrane, is affected by mutations of vacuolar SNARE and phospholipase in Arabidopsis."
    Saito C., Uemura T., Awai C., Tominaga M., Ebine K., Ito J., Ueda T., Abe H., Morita M.T., Tasaka M., Nakano A.
    Plant J. 68:64-73(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    Strain: cv. Columbia.
  11. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
    Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
    Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSYP22_ARATH
AccessioniPrimary (citable) accession number: P93654
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 12, 2003
Last sequence update: May 1, 1997
Last modified: February 17, 2016
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.