P93647 (LONP2_MAIZE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lon protease homolog 2, peroxisomal
EC=3.4.21.-

Gene names

Name:

LON1

Organism

Zea mays (Maize)

Taxonomic identifier

4577 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › PACMAD clade › Panicoideae › Andropogoneae › Zea

Protein attributes

Sequence length	885 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	ATP-dependent serine protease that mediates the selective degradation of misfolded and unassembled polypeptides in the peroxisomal matrix. Necessary for type 2 peroxisome targeting signal (PTS2)-containing protein processing and facilitates peroxisome matrix protein import By similarity. HAMAP-Rule MF_03121
Subcellular location	Peroxisome matrix By similarity.
Sequence similarities	Belongs to the peptidase S16 family. Contains 1 Lon domain.

Ontologies

Keywords
Cellular component	Peroxisome
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Protease Serine protease
Gene Ontology (GO)
Biological_process	growth Inferred from electronic annotation. Source: EnsemblPlants/Gramene lateral root development Inferred from electronic annotation. Source: EnsemblPlants/Gramene misfolded or incompletely synthesized protein catabolic process Inferred from electronic annotation. Source: InterPro protein import into peroxisome matrix, docking Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Cellular_component	peroxisomal matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent peptidase activity Inferred from electronic annotation. Source: InterPro serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 885	885	Lon protease homolog 2, peroxisomal HAMAP-Rule MF_03121		PRO_0000026737
Regions
	Domain	12 – 254	243	Lon
	Nucleotide binding	409 – 416	8	ATP Potential
	Motif	883 – 885	3	Microbody targeting signal Potential
Sites
	Active site	781	1	By similarity
	Active site	824	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P93647 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 331A321B56C75407	FASTA	885	97,733
	10 20 30 40 50 60 MSDSPVELPS RLAVLPFRNK VLLPGAIVRI RCTNPSSVKL VEQELWQKEE KGLIGVLPVR 70 80 90 100 110 120 DSEATAVGSL LSPGVGSDSG EGGSKVGGSA VESSKQDTKN GKEPIHWHSK GVAARALHLS 130 140 150 160 170 180 RGVEKPSGRV TYIVVLEGLC RFSVQELSAR GPYHVARVSR LDMTKTELEQ AEQDPDLIAL 190 200 210 220 230 240 SRQFKATAME LISVLEQKQK TVGRTKVLLD TVPVYRLADI FVASFEISFE EQLSMLDSVH 250 260 270 280 290 300 LKVRLSKATE LVDRHLQSIL VAEKITQKVE GQLSKSQKEF LLRQQMRAIK EELGDNDDDE 310 320 330 340 350 360 DDVAALERKM QNAGMPANIW KHAQREMRRL RKMQPQQPGY SSSRAYLELL ADLPWQKVSE 370 380 390 400 410 420 ERELDLRVAK ESLDQDHYGL TKVKQRIIEY LAVRKLKPDA RGPVLCFVGP PGVGKTSLAS 430 440 450 460 470 480 SIAKALNRKF IRISLGGVKD EADIRGHRRT YIGSMPGRLI DGLKRVSVSN PVMLLDEIDK 490 500 510 520 530 540 TGSDVRGDPA SALLEVLDPE QNKAFNDHYL NVPFDLSKVI FVATANRMQP IPPPLLDRME 550 560 570 580 590 600 IIELPGYTPE EKLKIAMKHL IPRVLEQHGL STTNLQIPEA MVKLVIERYT REAGVRNLER 610 620 630 640 650 660 NLAALARAAA VKVAEQVKTL RLGKEIQPIT TTLLDSRLAD GGEVEMEVIP MEHDISNTYE 670 680 690 700 710 720 NPSPMIVDEA MLEKVLGPPR FDDREAADRV ASPGVSVGLV WTSVGGEVQF VEATAMVGKG 730 740 750 760 770 780 DLHLTGQLGD VIKESAQLAL TWVRARAADL NLSPTSDINL LESRDIHIHF PAGAVPKDGP 790 800 810 820 830 840 SAGVTLVTAL VSLFSNRKVR ADTAMTGEMT LRGLVLPVGG VKDKVLAAHR YGIKRVILPE 850 860 870 880 RNLKDLSEVP LPILSDMEIL LVKRIEEVLD HAFEGRCPLR SRSKL

References

[1]	"Maize contains a Lon protease gene that can partially complement a yeast pim1-deletion mutant." Barakat S., Pearce D.A., Sherman F., Rapp W.D. Plant Mol. Biol. 37:141-154(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. B73.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U85494 mRNA. Translation: AAC50011.1.
PIR	T04321.
RefSeq	NP_001105903.1. NM_001112433.1.
UniGene	Zm.396.
3D structure databases
ProteinModelPortal	P93647.
SMR	P93647. Positions 693-876.
ModBase	Search...
Protein family/group databases
MEROPS	S16.003.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	732820.
KEGG	zma:732820.
Organism-specific databases
Gramene	P93647.
Phylogenomic databases
HOGENOM	HOG000261408.
KO	K01338.
OMA	IVEYFAT.
Family and domain databases
Gene3D	3.30.230.10. 1 hit.
HAMAP	MF_03121. lonp2_euk.
InterPro	IPR003593. AAA+_ATPase. IPR003959. ATPase_AAA_core. IPR027065. Lon_Prtase. IPR008269. Pept_S16_C. IPR004815. Pept_S16_lon. IPR003111. Pept_S16_N. IPR008268. Peptidase_S16_AS. IPR015947. PUA-like_domain. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
PANTHER	PTHR10046. PTHR10046. 1 hit.
Pfam	PF00004. AAA. 1 hit. PF02190. LON. 1 hit. PF05362. Lon_C. 1 hit. [Graphical view]
PIRSF	PIRSF001174. Lon_proteas. 1 hit.
SMART	SM00382. AAA. 1 hit. SM00464. LON. 1 hit. [Graphical view]
SUPFAM	SSF88697. PUA-like. 1 hit. SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00763. lon. 1 hit.
PROSITE	PS01046. LON_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LONP2_MAIZE

Accession

Primary (citable) accession number: P93647

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	May 1, 1997
Last modified:	May 1, 2013
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program