P93596 (CP51_WHEAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Obtusifoliol 14-alpha demethylase
EC=1.14.13.70

Alternative name(s):
CYPLI
Cytochrome P450 51
Cytochrome P450-LIA1

Gene names

Name:

CYP51

Organism

Triticum aestivum (Wheat)

Taxonomic identifier

4565 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Pooideae › Triticeae › Triticum

Protein attributes

Sequence length	453 AA.
Sequence status	Fragment.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the 14-alpha demethylation of obtusifoliol to 4 alpha-methyl-5 alpha-ergosta-8,14,24(28)-trien-3 beta-ol.
Catalytic activity	Obtusifoliol + 3 O2 + 3 NADPH = 4-alpha-methyl-5-alpha-ergosta-8,14,24(28)-trien-3-beta-ol + formate + 3 NADP+ + 4 H2O.
Cofactor	Heme group.
Pathway	Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 1/6.
Subcellular location	Membrane Potential. Membrane; Single-pass membrane protein.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Biological process	Lipid synthesis Steroid biosynthesis Sterol biosynthesis
Cellular component	Membrane
Ligand	Heme Iron Metal-binding NADP
Molecular function	Methyltransferase Monooxygenase Oxidoreductase Transferase
Gene Ontology (GO)
Biological process	sterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW sterol 14-demethylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 453	›453	Obtusifoliol 14-alpha demethylase		PRO_0000052014
Sites
	Metal binding	395	1	Iron (heme axial ligand) By similarity
Natural variations
	Natural variant	150	1	Missing in clone W516.
	Natural variant	175	1	L → Q in clone W516.
	Natural variant	443	1	N → S in clone W516.
	Natural variant	450	1	I → V in clone W516.
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	P93596 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 12A3D00952E3907D	FASTA	453	51,644
	10 20 30 40 50 60 RPPPTIPGAP VVGGLLRFLR GPIPLIRAEY ARLGPVFTVP ILTRRITFLI GPDVSAHFFK 70 80 90 100 110 120 SNESDMSQQE VYRFNVPTFG PGVVFDVDYQ VRQEQFRFFT EALRANKLRS YVDQMVAEAE 130 140 150 160 170 180 EYFSKWGESG TVDLKYELEH LIILTASRCL LGREVREKLF DDVSALFHDL DNGMLPISVI 190 200 210 220 230 240 FPYLPIPAHR RRDQARTRLA EIFATIIKSR KASGQSEEDM LQCFIDSKYK NGRQTTESEV 250 260 270 280 290 300 TGLLIAALFA GQHTSSITST WTGAYLLKFQ QYFAEAVEEQ KEVMKRHGDK IDHDILAEMD 310 320 330 340 350 360 VLYRCIKEAL RLHPPLIMLL RQSHSDFSVT TREGKEFDIP KGHIVATSPA FANRLPHIFK 370 380 390 400 410 420 NPDSYDPDRF AAGREEDKVA GAFSYISFGG GRHGCLGEPF AYLQIKAIWT HLLRNFEFEL 430 440 450 VSPFPENDWN AMVVGIKGEV MVNYKRRKLI VDN

References

[1]

"Cloning and functional expression in yeast of a cDNA coding for an obtusifoliol 14alpha-demethylase (CYP51) in wheat." Cabello-Hurtado F., Zimmerlin A., Rahier A., Taton M., Derose R., Nedelkina S., Batard Y., Durst F., Pallett K.E., Werck-Reichhart D. Biochem. Biophys. Res. Commun. 230:381-385(1997) [PubMed: 9016788] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Darius. Tissue: Shoot.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y09291 mRNA. Translation: CAA70475.1. Y09292 mRNA. Translation: CAA70476.1.
PIR	T06475.
3D structure databases
ProteinModelPortal	P93596.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
Gramene	P93596.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002403. Cyt_P450_E_grp-IV. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00465. EP450IV. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CP51_WHEAT

Accession

Primary (citable) accession number: P93596
Secondary accession number(s): P93595

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	May 1, 1997
Last modified:	September 21, 2011
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program