Reviewed,
UniProtKB/Swiss-Prot P93596 (CP51_WHEAT)
Last modified
November 25, 2008.
Version 59.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Cytochrome P450 51 EC=1.14.13.70 Alternative name(s): CYPLI P450-LIA1 Obtusifoliol 14-alpha demethylase | ||
| Gene names |
| ||
| Organism | Triticum aestivum (Wheat) | ||
| Taxonomic identifier | 4565 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Pooideae › Triticeae › Triticum |
Protein attributes
| Sequence length | 453 AA. |
| Sequence status | Fragment. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Function | Catalyzes the 14-alpha demethylation of obtusifoliol to 4 alpha-methyl-5 alpha-ergosta-8,14,24(28)-trien-3 beta-ol. |
| Catalytic activity | Obtusifoliol + 3 O(2) + 3 NADPH = 4-alpha-methyl-5-alpha-ergosta-8,14,24(28)-trien-3-beta-ol + formate + 3 NADP(+) + 4 H(2)O. |
| Cofactor | Heme group. |
| Pathway | Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 1/6. |
| Subcellular location | MembranePotential. Membrane; Single-pass membrane protein. |
| Sequence similarities | Belongs to the cytochrome P450 family. |
Ontologies
Keywords | |
|---|---|
| Biological process | Lipid synthesis Steroid biosynthesis Sterol biosynthesis |
| Cellular component | Membrane |
| Ligand | Heme Iron Metal-binding NADP |
| Molecular function | Methyltransferase Monooxygenase Oxidoreductase Transferase |
Gene Ontology (GO) | |
| Biological process | oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW sterol biosynthetic processInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | membrane Inferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | electron carrier activity Inferred from electronic annotation. Source: InterPro heme bindingInferred from electronic annotation. Source: InterPro iron ion bindingInferred from electronic annotation. Source: InterPro methyltransferase activityInferred from electronic annotation. Source: UniProtKB-KW sterol 14-demethylase activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | ‹1 – 453 | ›453 | Cytochrome P450 51 | PRO_0000052014 | |||||
Sites | |||||||||
| Metal binding | 395 | 1 | Iron (heme axial ligand) By similarity | ||||||
Natural variations | |||||||||
| Natural variant | 150 | 1 | Missing in clone W516. | ||||||
| Natural variant | 175 | 1 | L → Q in clone W516. | ||||||
| Natural variant | 443 | 1 | N → S in clone W516. | ||||||
| Natural variant | 450 | 1 | I → V in clone W516. | ||||||
Experimental info | |||||||||
| Non-terminal residue | 1 | 1 | |||||||
Sequences
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References
| [1] | "Cloning and functional expression in yeast of a cDNA coding for an obtusifoliol 14alpha-demethylase (CYP51) in wheat." Cabello-Hurtado F., Zimmerlin A., Rahier A., Taton M., Derose R., Nedelkina S., Batard Y., Durst F., Pallett K.E., Werck-Reichhart D. Biochem. Biophys. Res. Commun. 230:381-385(1997) [PubMed: 9016788] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Darius. Tissue: Shoot. |
Cross-references
Sequence databases | |
|---|---|
| Y09291 mRNA. Translation: CAA70475.1. Y09292 mRNA. Translation: CAA70476.1. | |
| PIR | T06475. |
3D structure databases | |
| HSSP | HSSP built from PDB template 1E9X based on UniProtKB P77901. |
| ModBase | Search... |
Organism-specific databases | |
| Gramene | P93596. |
Family and domain databases | |
| InterPro | IPR001128. Cyt_P450. IPR002403. Cyt_P450_E_grp-IV. [Graphical view] |
| Gene3D | G3DSA:1.10.630.10. Cyt_P450. 1 hit. |
| PANTHER | PTHR19383. Cyt_P450. 1 hit. |
| Pfam | PF00067. p450. 1 hit. [Graphical view] |
| PRINTS | PR00465. EP450IV. PR00385. P450. |
| PROSITE | PS00086. CYTOCHROME_P450. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | CP51_WHEAT | ||||||||
| Accession | Primary (citable) accession number: P93596 Secondary accession number(s): P93595 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | PPAP (Plant Proteome Annotation Project) | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with


