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P93594 (AMYB_WHEAT) Reviewed, UniProtKB/Swiss-Prot

Last modified October 19, 2011. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Beta-amylase
EC=3.2.1.2
Alternative name(s):
1,4-alpha-D-glucan maltohydrolase
Gene names
Name:BMY1
Synonyms:AMY1
OrganismTriticum aestivum (Wheat)
Taxonomic identifier4565 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Pooideae › Triticeae › Triticum

Protein attributes

Sequence length503 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

Hydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides so as to remove successive maltose units from the non-reducing ends of the chains.

Sequence similarities

Belongs to the glycosyl hydrolase 14 family.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
Polysaccharide degradation
   Molecular functionGlycosidase
Hydrolase
Gene Ontology (GO)
   Biological processpolysaccharide catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionbeta-amylase activity

Inferred from electronic annotation. Source: EC

cation binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 503503Beta-amylase
PRO_0000153941

Regions

Region379 – 3802Substrate binding By similarity

Sites

Active site1841Proton donor By similarity
Active site3781Proton acceptor By similarity
Binding site511Substrate By similarity
Binding site911Substrate By similarity
Binding site991Substrate By similarity
Binding site2931Substrate By similarity
Binding site2981Substrate By similarity
Binding site3401Substrate By similarity
Binding site4181Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
P93594 [UniParc].

Last modified May 1, 1997. Version 1.
Checksum: 71E9E1533A6C0F73

FASTA50356,611
        10         20         30         40         50         60 
MAGNMLANYV QVYVMLPLDV VSVDNKFEKG DEIRAQLKKL TEAGVDGVMI DVWWGLVEGK 

        70         80         90        100        110        120 
GPKAYDWSAY KQVFDLVHEA GLKLQAIMSF HQCGGNVGDV VNIPIPQWVR DVGATDPDIF 

       130        140        150        160        170        180 
YTNRGGTRNI EYLTLGVDDQ PLFHGRTAVQ MYADYMASFR ENMKKFLDAG TIVDIEVGLG 

       190        200        210        220        230        240 
PAGEMRYPSY PQSQGWVFPG IGEFICYDKY LEADFKAAAA KAGHPEWELP DDAGEYNDTP 

       250        260        270        280        290        300 
EKTQFFKDNG TYLTEKGKFF LSWYSNKLIK HGDKILDEAN KVFLGCRVQL AIKISGIHWW 

       310        320        330        340        350        360 
YRVPNHAAEL TAGYYNLDDR DGYRTIARML TRHHASMNFT CAEMRDSEQS EEAKSAPEEL 

       370        380        390        400        410        420 
VQQVLSAGWR EGLHVACENA LGRYDATAYN TILRNARPKG INKNGPPEHK LFGFTYLRLS 

       430        440        450        460        470        480 
NELLEGQNYA TFQTFVEKMH ANLGHDPSVD PVAPLERSKP EMPIEMILKA AQPKLEPFPF 

       490        500 
DKNTDLPVKD HTDVGDEVLV APV

« Hide

References

[1]"Nucleotide sequence of a cDNA from wheat leaves encoding ubiquitous beta-amylase."
Wagner G.B., Haeger K.-P., Ziegler P.
Plant Gene Register PGR96-123
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Star.
Tissue: Leaf.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X98504 mRNA. Translation: CAA67128.1.
UniGeneTa.3.

3D structure databases

ProteinModelPortalP93594.
SMRP93594. Positions 5-488.
ModBaseSearch...

Protein family/group databases

Allergome9594. Tri a bA.
CAZyGH14. Glycoside Hydrolase Family 14.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

GrameneP93594.

Family and domain databases

InterProIPR001554. Glyco_hydro_14.
IPR018238. Glyco_hydro_14_CS.
IPR001371. Glyco_hydro_14B_pln.
IPR013781. Glyco_hydro_subgr_catalytic.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
Gene3DG3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PfamPF01373. Glyco_hydro_14. 1 hit.
[Graphical view]
PRINTSPR00750. BETAAMYLASE.
PR00842. GLHYDLASE14B.
SUPFAMSSF51445. Glyco_hydro_cat. 1 hit.
PROSITEPS00506. BETA_AMYLASE_1. 1 hit.
PS00679. BETA_AMYLASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameAMYB_WHEAT
AccessionPrimary (citable) accession number: P93594
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: May 1, 1997
Last modified: October 19, 2011
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

SIMILARITY comments

Index of protein domains and families

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