Reviewed, UniProtKB/Swiss-Prot P93554 (NDK1_SACOF)

Names and origin

Protein names

Recommended name:
    Nucleoside diphosphate kinase 1
    EC=2.7.4.6
Alternative name(s):
    Nucleoside diphosphate kinase I
      Short name=NDP kinase I
      Short name=NDPK I
      Short name=NDK I
    PP18

Gene names

Name:

NDPK1

Organism

Saccharum officinarum (Sugarcane)

Taxonomic identifier

4547 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › PACCAD clade › Panicoideae › Andropogoneae › Saccharum › Saccharum officinarum complex

Protein attributes

Sequence length	149 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Also exhibits a kinase-like activity towards histone H1.
Catalytic activity	ATP + nucleoside diphosphate = ADP + nucleoside triphosphate.
Cofactor	Magnesium By similarity.
Post-translational modification	Autophosphorylated.
Sequence similarities	Belongs to the NDK family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	CTP biosynthetic process Inferred from electronic annotation. Source: InterPro GTP biosynthetic process Inferred from electronic annotation. Source: InterPro UTP biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside diphosphate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 149	149	Nucleoside diphosphate kinase 1		PRO_0000137143
Sites
	Active site	115	1	Pros-phosphohistidine intermediate By similarity
	Binding site	9	1	ATP By similarity
	Binding site	57	1	ATP By similarity
	Binding site	85	1	ATP By similarity
	Binding site	91	1	ATP By similarity
	Binding site	102	1	ATP By similarity
	Binding site	112	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P93554-1 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: F17167966E3EBC25	FASTA	149	16,601
	10 20 30 40 50 60 MESTFIMIKP DGVQRGLIGE IISRFEKKGF YLKALKLVNV ERSFAEKHYA DLSSKPFFQG 70 80 90 100 110 120 LVDYIISGPV VAMVWEGKSV VTTGRKIIGA TNPLVSEPGT IRGDFAVDIG RNVIHGSDSI 130 140 ESANKEIALW FPEGLADWQS SQHPWIYEK

References

[1]

"Characterization of a low molecular mass autophosphorylating protein in cultured sugarcane cells and its identification as a nucleoside diphosphate kinase." Moisyadi S., Dharmasiri S., Harrington H.M., Lukas T.J. Plant Physiol. 104:1401-1409(1994) [PubMed: 8016268] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Strain: cv. H50-7209.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U55019 mRNA. Translation: AAB40609.1.
3D structure databases
HSSP	HSSP built from PDB template 1NSQ based on UniProtKB P08879.
SMR	P93554. Positions 1-148.
ModBase	Search...
Organism-specific databases
Gramene	P93554.
Enzyme and pathway databases
BRENDA	2.7.4.6. 97260.
Family and domain databases
InterPro	IPR001564. Nuc_diP_kinase_core. [Graphical view]
Gene3D	G3DSA:3.30.70.141. NDK. 1 hit.
PANTHER	PTHR11349. Nuc_diP_kinase_core. 1 hit.
Pfam	PF00334. NDK. 1 hit. [Graphical view]
PRINTS	PR01243. NUCDPKINASE.
ProDom	PD001018. NDK. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00562. NDK. 1 hit. [Graphical view]
PROSITE	PS00469. NDP_KINASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NDK1_SACOF

Accession

Primary (citable) accession number: P93554

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	May 1, 1997
Last modified:	June 16, 2009
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)