Reviewed, UniProtKB/Swiss-Prot P93541 (DHE3_SOLLC)

Names and origin

Protein names

Recommended name:
    Glutamate dehydrogenase
      Short name=GDH
    EC=1.4.1.3
Alternative name(s):
    Legdh1

Gene names

Name:

GDH1

Organism

Solanum lycopersicum (Tomato) (Lycopersicon esculentum)

Taxonomic identifier

4081 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum › Lycopersicon

Protein attributes

Sequence length	412 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	L-glutamate + H(2)O + NAD(P)(+) = 2-oxoglutarate + NH(3) + NAD(P)H.
Subcellular location	Mitochondrion matrixPotential.
Tissue specificity	In roots, stems, leaves and flowers but not in fruits.
Sequence similarities	Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

Ontologies

Keywords
Cellular component	Mitochondrion
Ligand	NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	amino acid metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	binding Inferred from electronic annotation. Source: InterPro glutamate dehydrogenase [NAD(P)+] activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 412	412	Glutamate dehydrogenase		PRO_0000182748
Sites
	Active site	102	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P93541-1 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 83BC4138047A6BCA	FASTA	412	44,813
	10 20 30 40 50 60 MNALAATNRN FKLAARLLGL DSKLELSLLI PFREIKVECT IPKDDGTLAS FVGFRVQHDN 70 80 90 100 110 120 ARGPMKGGIR YHPEVDPDEV NALAQLMTWK TAVANIPYGG AKGGIGCSPS DLSISELERL 130 140 150 160 170 180 TRVFTQKIHD LIGIHTDVPA PDMGTNPQTM AWILDEYSKF HGYSPAVVTG KPVDLGGSLG 190 200 210 220 230 240 RDAATGRGAL FATEALLNEH GKSVAGQRFV IQGFGNVGSW AAKLIHEQGG KVVAVSDITG 250 260 270 280 290 300 AIKNEKGIDI ESLFKHVKET RGVKGFHDAH PIDANSILVE DCDVLIPAAL GGVINKDNHK 310 320 330 340 350 360 LKIKAKYIIE AANHPTDPEA DEILSKKGVT ILPDIYANSG GVTVSYFEWV QNIQGFMWDE 370 380 390 400 410 KKVNDELKTY MTRGFKDVKD MCKTHNCDLR MGAFTLGVNR VARATVLRGW EA

References

[1]	"Cloning and characterisation of a glutamate dehydrogenase cDNA from tomato (Lycopersicon esculentum L.)." Purnell M.P., Stewart G.R., Botella J.R. Gene 186:249-254(1997) [PubMed: 9074503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Pera.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U48695 mRNA. Translation: AAB39508.1.
PIR	JC6317.
UniGene	Les.20253
3D structure databases
HSSP	HSSP built from PDB template 1B26 based on UniProtKB P96110.
ModBase	Search...
Family and domain databases
InterPro	IPR006095. Glu/Leu/Phe/Val_DHase. IPR006096. Glu/Leu/Phe/Val_DHase_C. IPR006097. Glu/Leu/Phe/Val_DHase_dimer. IPR014362. Glu_DHase. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR11606:SF2. GLFV_DH. 1 hit.
Pfam	PF00208. ELFV_dehydrog. 1 hit. PF02812. ELFV_dehydrog_N. 1 hit. [Graphical view]
PIRSF	PIRSF000185. Glu_DH. 1 hit.
PRINTS	PR00082. GLFDHDRGNASE.
PROSITE	PS00074. GLFV_DEHYDROGENASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHE3_SOLLC

Accession

Primary (citable) accession number: P93541

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 1, 1997
Last modified:	November 25, 2008
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)