P93508 (CALR_RICCO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Calreticulin
Organism	Ricinus communis (Castor bean) [Complete proteome]
Taxonomic identifier	3988 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Malpighiales › Euphorbiaceae › Acalyphoideae › Acalypheae › Ricinus

Protein attributes

Sequence length	415 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin may interact transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER By similarity.
Subcellular location	Endoplasmic reticulum lumen.
Domain	Can be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity By similarity. The interaction with glycans occurs through a binding site in the globular lectin domain By similarity. The zinc binding sites are localized to the N-domain By similarity.
Sequence similarities	Belongs to the calreticulin family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum
Domain	Repeat Signal
Ligand	Calcium Lectin Metal-binding Zinc
Molecular function	Chaperone
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	protein folding Inferred from electronic annotation. Source: InterPro
Cellular_component	endoplasmic reticulum lumen Inferred from direct assay PubMed 11870775. Source: UniProtKB
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Chain	21 – 415	395	Calreticulin		PRO_0000004195
Regions
	Repeat	192 – 203	12	1-1
	Repeat	211 – 222	12	1-2
	Repeat	228 – 239	12	1-3
	Repeat	246 – 257	12	1-4
	Repeat	261 – 271	11	2-1
	Repeat	275 – 285	11	2-2
	Repeat	289 – 299	11	2-3
	Region	192 – 257	66	4 X approximate repeats
	Region	261 – 299	39	3 X approximate repeats
	Motif	412 – 415	4	Prevents secretion from ER Potential
	Compositional bias	353 – 410	58	Asp/Glu/Lys-rich
Sites
	Binding site	110	1	Carbohydrate By similarity
	Binding site	112	1	Carbohydrate By similarity
	Binding site	129	1	Carbohydrate By similarity
	Binding site	136	1	Carbohydrate By similarity
	Binding site	319	1	Carbohydrate By similarity
Amino acid modifications
	Glycosylation	52	1	N-linked (GlcNAc...) Potential
	Glycosylation	152	1	N-linked (GlcNAc...) Potential
	Disulfide bond	106 ↔ 138		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P93508 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: DD5F452E76CC7F8C	FASTA	415	47,522
	10 20 30 40 50 60 MANPKSLSLF LLSLLAIASA EVFFEERFED GWENRWVKSD WKKDENTAGE WNYTSGKWNG 70 80 90 100 110 120 DPNDKGIQTS EDYRFYAISA EFPEFSNKDK TLVFQFSVKH EQKLDCGGGY MKLLSSSTDQ 130 140 150 160 170 180 KKFGGDTPYS IMFGPDICGY STKKVHAILN YNDTNHLIKK EVPCETDQLT HVYTLVIRPD 190 200 210 220 230 240 ATYSILIDNV EKQTGSLYTD WDLLPPKKIK DPEAKKPEDW DEKEYIPDPE DKKPEGYDDI 250 260 270 280 290 300 PKEIPDPDAK KPEDWDDEED GEWTAPTIAN PEYKGPWKPK KIKNPNYKGK WKAPMIDNPD 310 320 330 340 350 360 FKDDPEIYVY PNLKYVGIEL WQVKSGTLFD NVLICNDPEY AKQLAEETWG KNKDAEKAAF 370 380 390 400 410 EEAEKKKEEE ESKDDPADSD ADEDDDDADD TEGEDDGESK SDAAEDSAED VHDEL

References

[1]	"Cloning and characterization of the calreticulin gene from Ricinus communis L." Coughlan S.J., Hastings C., Winfrey R. Jr. Plant Mol. Biol. 34:897-911(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	U74631 Genomic DNA. Translation: AAB71420.1. U74630 mRNA. Translation: AAB71419.1.
PIR	T10172.
RefSeq	XP_002512501.1. XM_002512455.1.
3D structure databases
ProteinModelPortal	P93508.
SMR	P93508. Positions 207-307.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	8269812.
KEGG	rcu:RCOM_1434770.
Phylogenomic databases
KO	K08057.
ProtClustDB	CLSN2719690.
Family and domain databases
Gene3D	2.10.250.10. 1 hit. 2.60.120.200. 1 hit.
InterPro	IPR001580. Calret/calnex. IPR018124. Calret/calnex_CS. IPR009169. Calreticulin. IPR009033. Calreticulin/calnexin_P_dom. IPR008985. ConA-like_lec_gl_sf. IPR013320. ConA-like_subgrp. [Graphical view]
PANTHER	PTHR11073. PTHR11073. 1 hit.
Pfam	PF00262. Calreticulin. 1 hit. [Graphical view]
PIRSF	PIRSF002356. Calreticulin. 1 hit.
PRINTS	PR00626. CALRETICULIN.
SUPFAM	SSF63887. Calret_calnex_P. 1 hit. SSF49899. ConA_like_lec_gl. 1 hit.
PROSITE	PS00803. CALRETICULIN_1. 1 hit. PS00804. CALRETICULIN_2. 1 hit. PS00805. CALRETICULIN_REPEAT. 2 hits. PS00014. ER_TARGET. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CALR_RICCO

Accession

Primary (citable) accession number: P93508

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 1, 1997
Last modified:	April 3, 2013
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program