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Reviewed, UniProtKB/Swiss-Prot P93438 (METK2_ORYSJ)

Last modified September 22, 2009. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    S-adenosylmethionine synthetase 2
      Short name=AdoMet synthetase 2
    EC=2.5.1.6
Alternative name(s):
    Methionine adenosyltransferase 2
      Short name=MAT 2
Gene names
Name: SAM2
Synonyms: SAMS2
Ordered Locus Names: Os01g0323600, LOC_Os01g22010
ORF Names: OSJNBa0011P19.8
OrganismOryza sativa subsp. japonica (Rice)
Taxonomic identifier39947 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza

Protein attributes

Sequence length394 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at transcript level.

General annotation (Comments)

Function

Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme By similarity.

Catalytic activity

ATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine.

Cofactor

Binds 2 divalent ions per subunit. Magnesium or cobalt By similarity.

Binds 1 potassium ion per subunit By similarity.

Pathway

Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1.

Subunit structure

Homotetramer By similarity.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the AdoMet synthetase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 394394S-adenosylmethionine synthetase 2
PRO_0000174471

Regions

Nucleotide binding121 – 1266ATP Potential
Nucleotide binding269 – 2768ATP Potential

Sites

Metal binding191Magnesium By similarity
Metal binding451Potassium By similarity
Metal binding2731Potassium By similarity
Metal binding2811Magnesium By similarity
Binding site1491ATP Potential

Sequences

Sequence LengthMass (Da)Tools
P93438-1 [UniParc].

Last modified May 1, 1997. Version 1.
Checksum: 081E9F3E65694EB7

FASTA39442,901
        10         20         30         40         50         60 
MAAETFLFTS ESVNEGHPDK LCDQVSDAVL DACLAQDPDS KVACETCTKT NMVMVFGEIT 

        70         80         90        100        110        120 
TKATVDYEKI VRDTCRGIGF VSDDVGLDAD RCKVLVNIEQ QSPDIAQGVH GHFTKRPEEI 

       130        140        150        160        170        180 
GAGDQGHMFG YATDETPELM PLSHVLATKL GARLTEVRKN GTCAWLRPDG KTQVTVEYLN 

       190        200        210        220        230        240 
DAGAMVPVRV HTVLISTQHD ETVTNDEIAA DLKEHVIKPV IPDKYLDEKT IFHLNPSGRF 

       250        260        270        280        290        300 
VIGGPHGDAG LTGRKIIIDT YGGWGAHGGG AFSGKDPTKV DRSGAYIARQ AAKSIVASGL 

       310        320        330        340        350        360 
ARRCIVQVSY AIGVPEPLSV FVDSYGTGKI PDKEILKIVK ENFDFRPGMM TINLDLKRGG 

       370        380        390 
NRFIKTAAYG HFGREDPDFT WEVVKPLKYE KASS 

« Hide

Cross-references

Sequence databases

U82833 mRNA. Translation: AAC05590.1.
AP003211 Genomic DNA. Translation: BAC65881.1.

3D structure databases

HSSPHSSP built from PDB template 1QM4 based on UniProtKB P13444.
ModBaseSearch...

2-D gel databases

ANU-2DPAGEP93438.

Genome annotation databases

NMPDRfig|39947.1.peg.305.

Organism-specific databases

GrameneP93438.

Enzyme and pathway databases

BioCycMetaCyc:MON-13932.

Family and domain databases

InterProIPR002133. S-AdoMet_synthetase.
[Graphical view]
PANTHERPTHR11964. S-AdoMet_synt. 1 hit.
PfamPF02773. S-AdoMet_synt_C. 1 hit.
PF02772. S-AdoMet_synt_M. 1 hit.
PF00438. S-AdoMet_synt_N. 1 hit.
[Graphical view]
PIRSFPIRSF000497. MAT. 1 hit.
TIGRFAMsTIGR01034. metK. 1 hit.
PROSITEPS00376. ADOMET_SYNTHETASE_1. 1 hit.
PS00377. ADOMET_SYNTHETASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMETK2_ORYSJ
AccessionPrimary (citable) accession number: P93438
Secondary accession number(s): Q7F660
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: May 1, 1997
Last modified: September 22, 2009
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

Relevant documents

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents