P93438 (METK2_ORYSJ) Reviewed, UniProtKB/Swiss-Prot
Last modified
September 21, 2011.
Version 77.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: S-adenosylmethionine synthase 2 Short name=AdoMet synthase 2 EC=2.5.1.6 Alternative name(s): Methionine adenosyltransferase 2 Short name=MAT 2 | ||||||||
| Gene names |
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| Organism | Oryza sativa subsp. japonica (Rice) | ||||||||
| Taxonomic identifier | 39947 [NCBI] | ||||||||
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza |
Protein attributes
| Sequence length | 394 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at transcript level |
General annotation (Comments)
| Function | Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme By similarity. |
| Catalytic activity | ATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine. |
| Cofactor | Binds 2 divalent ions per subunit. Magnesium or cobalt By similarity. Binds 1 potassium ion per subunit By similarity. |
| Pathway | |
| Subunit structure | Homotetramer By similarity. |
| Subcellular location | Cytoplasm By similarity. |
| Sequence similarities | Belongs to the AdoMet synthase family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | One-carbon metabolism |
| Cellular component | Cytoplasm |
| Ligand | ATP-binding Cobalt Magnesium Metal-binding Nucleotide-binding Potassium |
| Molecular function | Transferase |
| Gene Ontology (GO) | |
| Biological process | S-adenosylmethionine biosynthetic process Inferred from electronic annotation. Source: InterPro one-carbon metabolic processInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion bindingInferred from electronic annotation. Source: UniProtKB-KW methionine adenosyltransferase activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 394 | 394 | S-adenosylmethionine synthase 2 | PRO_0000174471 | |||||
Regions | |||||||||
| Nucleotide binding | 121 – 126 | 6 | ATP Potential | ||||||
| Nucleotide binding | 269 – 276 | 8 | ATP Potential | ||||||
Sites | |||||||||
| Metal binding | 19 | 1 | Magnesium By similarity | ||||||
| Metal binding | 45 | 1 | Potassium By similarity | ||||||
| Metal binding | 273 | 1 | Potassium By similarity | ||||||
| Metal binding | 281 | 1 | Magnesium By similarity | ||||||
| Binding site | 149 | 1 | ATP Potential | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Structure and expression of two cDNAs encoding S-adenosyl-L-methionine synthetase of rice (Oryza sativa L.)." Lee J.-H., Chae H.S., Lee J.-H., Hwang B., Hahn K.W., Kang B.G., Kim W.T. Biochim. Biophys. Acta 1354:13-18(1997) [PubMed: 9375784] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [2] | "The genome sequence and structure of rice chromosome 1." Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M. Gojobori T.Nature 420:312-316(2002) [PubMed: 12447438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | U82833 mRNA. Translation: AAC05590.1. AP003211 Genomic DNA. Translation: BAC65881.1. |
3D structure databases | |
| ProteinModelPortal | P93438. |
| SMR | P93438. Positions 5-389. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | P93438. |
2D gel databases | |
| ANU-2DPAGE | P93438. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblPlants | LOC_Os01g22010.1; LOC_Os01g22010.1; LOC_Os01g22010. LOC_Os01g22010.2; LOC_Os01g22010.2; LOC_Os01g22010. LOC_Os01g22010.3; LOC_Os01g22010.3; LOC_Os01g22010. LOC_Os01g22010.4; LOC_Os01g22010.4; LOC_Os01g22010. |
| NMPDR | fig|39947.1.peg.305. |
Organism-specific databases | |
| Gramene | P93438. |
Phylogenomic databases | |
| GeneTree | EPGT00050000019410. |
| OMA | NRFIKTA. |
Enzyme and pathway databases | |
| BioCyc | MetaCyc:MONOMER-13932. |
Family and domain databases | |
| InterPro | IPR022631. ADOMET_SYNTHASE_CS. IPR022630. S-AdoMet_synt_C. IPR022629. S-AdoMet_synt_central. IPR022628. S-AdoMet_synt_N. IPR002133. S-AdoMet_synthetase. IPR022636. S-AdoMet_synthetase_sfam. [Graphical view] |
| PANTHER | PTHR11964. S-AdoMet_synt. 1 hit. |
| Pfam | PF02773. S-AdoMet_synt_C. 1 hit. PF02772. S-AdoMet_synt_M. 1 hit. PF00438. S-AdoMet_synt_N. 1 hit. [Graphical view] |
| PIRSF | PIRSF000497. MAT. 1 hit. |
| SUPFAM | SSF55973. S-AdoMet_synt. 3 hits. |
| TIGRFAMs | TIGR01034. MetK. 1 hit. |
| PROSITE | PS00376. ADOMET_SYNTHASE_1. 1 hit. PS00377. ADOMET_SYNTHASE_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | METK2_ORYSJ | ||||||||
| Accession | Primary (citable) accession number: P93438 Secondary accession number(s): Q7F660 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Plant Protein Annotation Program | ||||||||
Relevant documents
| Oryza sativa (rice) Index of Oryza sativa entries and their corresponding gene designations |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with