P93400 (PLDA1_TOBAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phospholipase D alpha 1
Short name=PLD alpha 1
EC=3.1.4.4

Alternative name(s):
Choline phosphatase 1
Phosphatidylcholine-hydrolyzing phospholipase D 1

Gene names

Name:

PLD1

Organism

Nicotiana tabacum (Common tobacco)

Taxonomic identifier

4097 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana

Protein attributes

Sequence length	808 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Hydrolyzes glycerol-phospholipids at the terminal phosphodiesteric bond. Plays an important role in various cellular processes.
Catalytic activity	A phosphatidylcholine + H2O = choline + a phosphatidate.
Cofactor	Calcium By similarity.
Domain	C2 domain is a calcium-binding fold, and the binding promotes the protein association with membranes. A lower affinity toward calcium can be anticipated for PLD alpha due to the absence of two potential calcium ligands.
Sequence similarities	Belongs to the phospholipase D family. C2-PLD subfamily. Contains 1 C2 domain. Contains 2 PLD phosphodiesterase domains.

Ontologies

Keywords
Biological process	Lipid degradation
Domain	Repeat
Ligand	Calcium
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW phosphatidylcholine metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	membrane Inferred from electronic annotation. Source: InterPro
Molecular function	NAPE-specific phospholipase D activity Inferred from electronic annotation. Source: EC calcium ion binding Inferred from electronic annotation. Source: InterPro phospholipase D activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 808	808	Phospholipase D alpha 1		PRO_0000218823
Regions
	Domain	1 – 109	109	C2
	Domain	326 – 364	39	PLD phosphodiesterase 1
	Domain	654 – 681	28	PLD phosphodiesterase 2
Sites
	Active site	331	1	Potential
	Active site	333	1	Potential
	Active site	338	1	Potential
	Active site	659	1	Potential
	Active site	661	1	Potential
	Active site	666	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	P93400 [UniParc]. Last modified January 1, 1998. Version 2. Checksum: 2937FCA5D5C60B6A	FASTA	808	91,967
	10 20 30 40 50 60 MAQILLHGTL HVTIYEVDNL QKEGGGHFFS KIKEHVEETI GFGKGTPAIY ATVDLEKARV 70 80 90 100 110 120 GRTRKIKNEP NNPRWYESFH IYCAHMASNV IFTVKDDNPI GATLIGRAYV PVEELLEGEE 130 140 150 160 170 180 IDKWVEILDR EMNPIAEGSK IHVKLQFFDV SRDPNWERGI RSSKYPGVPY TFFAQRTGCR 190 200 210 220 230 240 VSLYQDAHVP DNFIPKIPLS GGKYYEPHRC WEDIFDAIIN AKHLIYITGW SVYTEITLVR 250 260 270 280 290 300 DSRRQKPGGD ITLGELLKKK ASEGVKVLML VWDDRTSVGL LKKDGLMATH DQETEQFFQG 310 320 330 340 350 360 TEVNCVLCPR NPDDGGSIVQ SLQIGTMFTH HQKIVVVDSE LPSGESEKRR ILSFVGGIDL 370 380 390 400 410 420 CDGRYDTPFH SLFRTLDTAH HDDFHQPNFP DGAITKGGPR EPWHDIHSRL EGPIAWDVLF 430 440 450 460 470 480 NFEQRWRKQG GKDVLVNFRE LDDIIIPPSP VMHLDDSETW NVQLFRSIDE GAAFGFPETP 490 500 510 520 530 540 EDAAKAGLVS GXDNIIDRSI QDAYIHAIRR AKNFIYIENQ YFLGSSYDWQ SDDIKVEDIG 550 560 570 580 590 600 ALHVIPKELA LKIVSKIEAG ERFTVYVVVP MWPEGIPESA SVQAILDWQR RTMEMMYKHI 610 620 630 640 650 660 VQALNAKGIE EDPRNYLTFF CIGNREVKKS GAYEPSETPE PDSDYIRAQE ARRFMIYVHS 670 680 690 700 710 720 KMMIVDDEYI IVGSANINQR SMDGARDSEI AMGAYQPHHL ATREPARGQI HGFRMALWYE 730 740 750 760 770 780 HLGMLDETFL HPESEECVSK VNRMADKYWD LYSSESLERD LPGHLLRYPI GVASEGDVTE 790 800 LPGAEHFPDT KARVLGTKSD YLPPILTT

References

[1]	Lein W., Saalbach G. Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. SR1. Tissue: Leaf.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z84822 mRNA. Translation: CAB06620.1.
PIR	T04092.
3D structure databases
ProteinModelPortal	P93400.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR000008. C2_Ca-dep. IPR008973. C2_Ca/lipid-bd_dom_CaLB. IPR015679. PLipase_D. IPR001736. PLipase_D/transphosphatidylase. IPR024632. PLipase_D_C. IPR011402. PLipase_D_pln. [Graphical view]
PANTHER	PTHR18896. Phospholipase_D. 1 hit.
Pfam	PF00168. C2. 1 hit. PF12357. PLD_C. 1 hit. PF00614. PLDc. 2 hits. [Graphical view]
PIRSF	PIRSF036470. PLD_plant. 1 hit.
SMART	SM00239. C2. 1 hit. SM00155. PLDc. 2 hits. [Graphical view]
SUPFAM	SSF49562. C2_CaLB. 1 hit.
PROSITE	PS50004. C2. False negative. PS50035. PLD. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PLDA1_TOBAC

Accession

Primary (citable) accession number: P93400
Secondary accession number(s): P93399

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	January 1, 1998
Last modified:	October 19, 2011
This is version 62 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program