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P93349 (XTH_TOBAC) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable xyloglucan endotransglucosylase/hydrolase protein

EC=2.4.1.207
Gene names
Name:XTH
Synonyms:EXGT
OrganismNicotiana tabacum (Common tobacco)
Taxonomic identifier4097 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

Protein attributes

Sequence length295 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues.

Catalytic activity

Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan.

Subcellular location

Secretedcell wall. Secretedextracellular spaceapoplast Ref.2.

Post-translational modification

Contains at least one intrachain disulfide bond essential for its enzymatic activity By similarity.

Sequence similarities

Belongs to the glycosyl hydrolase 16 family. XTH group 1 subfamily.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 Potential
Chain22 – 295274Probable xyloglucan endotransglucosylase/hydrolase protein
PRO_0000011840

Sites

Active site1061Nucleophile By similarity
Active site1101Proton donor By similarity

Amino acid modifications

Glycosylation1141N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
P93349 [UniParc].

Last modified May 1, 1997. Version 1.
Checksum: D379980DC2DA1765

FASTA29533,882
        10         20         30         40         50         60 
MGVKGLLFSI VLINLSLLGL CGYPRKPVDV PFWKNYEPSW ASHHIKYLSG GSTVDLVLDR 

        70         80         90        100        110        120 
SSGAGFQSKK SYLFGHFSMK LKLVGGDSAG VVTAFYLSSN NAEHDEIDFE FLGNRTGQPY 

       130        140        150        160        170        180 
ILQTNVFTGG KGDREQRIYL WFDPTKGYHS YSVLWNTFQI VIFVDDVPIR AFKNSKDLGV 

       190        200        210        220        230        240 
KFPFNQPMKI YSSLWDADDW ATRGGLEKTD WSNAPFTASY TSFHVDGCEA ATPQEVQVCN 

       250        260        270        280        290 
TKGMRWWDQK AFQDLDALQY RRLRWVRQKY TIYNYCTDRK RYPTLPPECT KDRDI 

« Hide

References

[1]"Endo-xyloglucan transferase (EXGT)."
Nishitani K., Okazawa K., Takeda S., Asada K., Kato I.
Submitted (JUL-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Endoxyloglucan transferase is localized both in the cell plate and in the secretory pathway destined for the apoplast in tobacco cells."
Yokoyama R., Nishitani K.
Plant Cell Physiol. 42:292-300(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D86730 mRNA. Translation: BAA13163.1.

3D structure databases

ProteinModelPortalP93349.
SMRP93349. Positions 27-295.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH16. Glycoside Hydrolase Family 16.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

BRENDA2.4.1.207. 3645.

Family and domain databases

Gene3D2.60.120.200. 1 hit.
InterProIPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR000757. Glyco_hydro_16.
IPR008263. Glycoside_hydrolase_16_AS.
IPR016455. XET.
IPR010713. XET_C.
[Graphical view]
PfamPF00722. Glyco_hydro_16. 1 hit.
PF06955. XET_C. 1 hit.
[Graphical view]
PIRSFPIRSF005604. XET. 1 hit.
SUPFAMSSF49899. SSF49899. 1 hit.
PROSITEPS01034. GLYCOSYL_HYDROL_F16. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameXTH_TOBAC
AccessionPrimary (citable) accession number: P93349
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2003
Last sequence update: May 1, 1997
Last modified: February 19, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries