ID   NO16_MEDTR              Reviewed;         180 AA.
AC   P93328;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1997, sequence version 1.
DT   27-MAR-2024, entry version 85.
DE   RecName: Full=Early nodulin-16 {ECO:0000303|PubMed:9526510};
DE            Short=N-16 {ECO:0000303|PubMed:9526510};
DE   Flags: Precursor;
GN   Name=ENOD16 {ECO:0000303|PubMed:9526510};
OS   Medicago truncatula (Barrel medic) (Medicago tribuloides).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; Hologalegina; IRL clade; Trifolieae; Medicago.
OX   NCBI_TaxID=3880;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=cv. Jemalong;
RX   PubMed=9526510; DOI=10.1023/a:1005916821224;
RA   Greene E.A., Erard M., Dedieu A., Barker D.G.;
RT   "MtENOD16 and 20 are members of a family of phytocyanin-related early
RT   nodulins.";
RL   Plant Mol. Biol. 36:775-783(1998).
RN   [2]
RP   IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION, DEVELOPMENTAL
RP   STAGE, AND TISSUE SPECIFICITY.
RC   STRAIN=cv. Jemalong A17;
RX   PubMed=14760646; DOI=10.1002/elps.200305711;
RA   Catalano C.M., Lane W.S., Sherrier D.J.;
RT   "Biochemical characterization of symbiosome membrane proteins from Medicago
RT   truncatula root nodules.";
RL   Electrophoresis 25:519-531(2004).
CC   -!- FUNCTION: May act as a carbohydrate transporter. {ECO:0000305}.
CC   -!- SUBCELLULAR LOCATION: Symbiosome {ECO:0000269|PubMed:14760646}. Cell
CC       membrane {ECO:0000255}; Lipid-anchor, GPI-anchor {ECO:0000255}.
CC   -!- TISSUE SPECIFICITY: Expressed in developing nodules upon symbiosis with
CC       Sinorhizobium meliloti. {ECO:0000303|PubMed:14760646}.
CC   -!- DEVELOPMENTAL STAGE: Expressed during the early stages of nodule
CC       development in cells with growing infection threads upon symbiosis with
CC       Sinorhizobium meliloti. {ECO:0000303|PubMed:14760646}.
CC   -!- SIMILARITY: Belongs to the early nodulin-like (ENODL) family.
CC       {ECO:0000305}.
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DR   EMBL; X99466; CAA67829.1; -; Genomic_DNA.
DR   AlphaFoldDB; P93328; -.
DR   SMR; P93328; -.
DR   PaxDb; 3880-AES92507; -.
DR   EnsemblPlants; AES92507; AES92507; MTR_4g130780.
DR   Gramene; AES92507; AES92507; MTR_4g130780.
DR   eggNOG; ENOG502SECN; Eukaryota.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0098552; C:side of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0043659; C:symbiosome; IEA:UniProtKB-SubCell.
DR   GO; GO:0009055; F:electron transfer activity; IEA:InterPro.
DR   GO; GO:0009877; P:nodulation; IEA:UniProtKB-KW.
DR   CDD; cd04216; Phytocyanin; 1.
DR   Gene3D; 2.60.40.420; Cupredoxins - blue copper proteins; 1.
DR   InterPro; IPR008972; Cupredoxin.
DR   InterPro; IPR039391; Phytocyanin-like.
DR   InterPro; IPR003245; Phytocyanin_dom.
DR   PANTHER; PTHR33021; BLUE COPPER PROTEIN; 1.
DR   PANTHER; PTHR33021:SF519; EARLY NODULIN-55-2; 1.
DR   Pfam; PF02298; Cu_bind_like; 1.
DR   SUPFAM; SSF49503; Cupredoxins; 1.
DR   PROSITE; PS51485; PHYTOCYANIN; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Disulfide bond; Glycoprotein; GPI-anchor; Lipoprotein;
KW   Membrane; Nodulation; Signal.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   CHAIN           23..154
FT                   /note="Early nodulin-16"
FT                   /id="PRO_0000002876"
FT   PROPEP          155..180
FT                   /note="Removed in mature form"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000457727"
FT   DOMAIN          25..129
FT                   /note="Phytocyanin"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00818"
FT   LIPID           154
FT                   /note="GPI-anchor amidated serine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        67
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   CARBOHYD        152
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   DISULFID        83..117
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00818"
SQ   SEQUENCE   180 AA;  20026 MW;  F97452B75007CB6E CRC64;
     MASSSPILLM IIFSMWLLIS HSESTDYLIG DSHNSWKVPL PSRRAFARWA SAHEFTVGDT
     ILFEYDNETE SVHEVNEHDY IMCHTNGEHV EHHDGNTKVV LDKIGVYHFI SGTKRHCKMG
     LKLAVVVQNK HDLVLPPLIT MPMPPSPSPS PNSSGNKGGA AGLGFIMWLG VSLVMMMFLI
//