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Protein

NADH-ubiquinone oxidoreductase chain 4

Gene

ND4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity).By similarity

Catalytic activityi

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

NAD, Ubiquinone

Enzyme and pathway databases

BioCyciARA:ATMG00580-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-ubiquinone oxidoreductase chain 4 (EC:1.6.5.3)
Alternative name(s):
NADH dehydrogenase subunit 4
Gene namesi
Name:ND4
Synonyms:NAD4
Ordered Locus Names:AtMg00580
Encoded oniMitochondrion
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Mitochondrion

Organism-specific databases

TAIRiATMG00580.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei9 – 2921HelicalSequence analysisAdd
BLAST
Transmembranei39 – 5921HelicalSequence analysisAdd
BLAST
Transmembranei89 – 10921HelicalSequence analysisAdd
BLAST
Transmembranei118 – 13821HelicalSequence analysisAdd
BLAST
Transmembranei173 – 19321HelicalSequence analysisAdd
BLAST
Transmembranei214 – 23421HelicalSequence analysisAdd
BLAST
Transmembranei245 – 26521HelicalSequence analysisAdd
BLAST
Transmembranei272 – 29221HelicalSequence analysisAdd
BLAST
Transmembranei335 – 35521HelicalSequence analysisAdd
BLAST
Transmembranei367 – 38721HelicalSequence analysisAdd
BLAST
Transmembranei413 – 43321HelicalSequence analysisAdd
BLAST
Transmembranei457 – 47721HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 495495NADH-ubiquinone oxidoreductase chain 4PRO_0000117889Add
BLAST

Proteomic databases

PRIDEiP93313.

Interactioni

Subunit structurei

Complex I is composed of at least 49 different subunits.

Protein-protein interaction databases

IntActiP93313. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliP93313.
SMRiP93313. Positions 12-490.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I subunit 4 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000100683.
InParanoidiP93313.
KOiK03881.

Family and domain databases

InterProiIPR010227. NADH_Q_OxRdtase_chainM/4.
IPR003918. NADH_UbQ_OxRdtase.
IPR001750. ND/Mrp_mem.
[Graphical view]
PfamiPF00361. Proton_antipo_M. 1 hit.
[Graphical view]
PRINTSiPR01437. NUOXDRDTASE4.
TIGRFAMsiTIGR01972. NDH_I_M. 1 hit.

Sequencei

Sequence statusi: Complete.

P93313-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLEHFCECYF NLSGLILCPV LGSIILLFIP NSRIRLIRLI GLCASLITFL
60 70 80 90 100
YSLVLWIQFD SSTAKFQFVE SLRWLPYENI NFYLGIDGIS LFFVILTTFL
110 120 130 140 150
IPICILVGWS GMRSYGKEYI IAFLICEFLM IAVFCMLDLL LFYVFPESVL
160 170 180 190 200
IPMFIIIGVW GSRQRKIKAA YQFFLYTLLG SLFMLLAILL ILFQTGTTDL
210 220 230 240 250
QILLTTEFSE RRQIFLWIAF FASFAVKVPM VPVHIWLPEA HVEAPTAGSV
260 270 280 290 300
ILAGILLKFG TYGFLRFSIP MFPEATLCFT PFIYTLSAIA IIYTSLTTLR
310 320 330 340 350
QIDLKKIIAY SSVAHMNLVT IGMFSPNIQG IGGSILLMLS HGLVSSALFL
360 370 380 390 400
CVGVLYDRHK TRLVRYYGGL VSTMPNFSTI FFSFTLANMS LPGTSSFIGE
410 420 430 440 450
FLILVGAFQR NSLVATLAAL GMILGAAYSL WLYNRVVSGN LKPDFLHKFS
460 470 480 490
DLNGREVFIF IPFLVGLVWM GVYPKVFLDC MHTSVSNLVQ HGKFH
Length:495
Mass (Da):55,830
Last modified:March 1, 2004 - v2
Checksum:i246135C86467F445
GO

RNA editingi

Edited at positions 10, 25, 36, 53, 55, 56, 66, 106, 121, 126, 135, 150, 203, 220, 256, 262, 279, 299, 337, 345, 377, 391, 452, 458, 469, 473 and 478.1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y08501 Genomic DNA. Translation: CAA69742.3. Sequence problems.
AC007729 Genomic DNA. No translation available.
RefSeqiNP_085518.1. NC_001284.2.

Genome annotation databases

GeneIDi814596.
KEGGiath:ArthMp051.

Keywords - Coding sequence diversityi

RNA editing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y08501 Genomic DNA. Translation: CAA69742.3. Sequence problems.
AC007729 Genomic DNA. No translation available.
RefSeqiNP_085518.1. NC_001284.2.

3D structure databases

ProteinModelPortaliP93313.
SMRiP93313. Positions 12-490.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP93313. 1 interaction.

Proteomic databases

PRIDEiP93313.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi814596.
KEGGiath:ArthMp051.

Organism-specific databases

TAIRiATMG00580.

Phylogenomic databases

HOGENOMiHOG000100683.
InParanoidiP93313.
KOiK03881.

Enzyme and pathway databases

BioCyciARA:ATMG00580-MONOMER.

Miscellaneous databases

PROiP93313.

Family and domain databases

InterProiIPR010227. NADH_Q_OxRdtase_chainM/4.
IPR003918. NADH_UbQ_OxRdtase.
IPR001750. ND/Mrp_mem.
[Graphical view]
PfamiPF00361. Proton_antipo_M. 1 hit.
[Graphical view]
PRINTSiPR01437. NUOXDRDTASE4.
TIGRFAMsiTIGR01972. NDH_I_M. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNU4M_ARATH
AccessioniPrimary (citable) accession number: P93313
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: March 1, 2004
Last modified: November 11, 2015
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

A stretch of 270 kb of the mitochondrial genome is duplicated within the centromere of chromosome 2 resulting in the duplication of the gene. The expression of the duplicated gene (At2g07717) is not demonstrated. It is also probably not RNA edited and therefore differs in all the positions known to be edited.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.